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司美格鲁肽对2型糖尿病合并多囊卵巢综合征患者血清超敏C反应蛋白及IV型胶原蛋白的影响 被引量:1
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作者 张文龙 魏东 郑莉 《川北医学院学报》 CAS 2023年第12期1639-1642,共4页
目的:探讨司美格鲁肽对2型糖尿病(T2DM)合并多囊卵巢综合征(PCOS)患者血清超敏C反应蛋白(hs-CRP)及IV型胶原蛋白(Col IV)的影响。方法:选取30例初诊T2DM合并PCOS患者为研究对象,给予司美格鲁肽治疗16周。分析比较患者治疗前后血糖、血... 目的:探讨司美格鲁肽对2型糖尿病(T2DM)合并多囊卵巢综合征(PCOS)患者血清超敏C反应蛋白(hs-CRP)及IV型胶原蛋白(Col IV)的影响。方法:选取30例初诊T2DM合并PCOS患者为研究对象,给予司美格鲁肽治疗16周。分析比较患者治疗前后血糖、血脂、体重、性激素相关指标及hs-CRP和Col IV的变化,并探究司美格鲁肽治疗后hs-CRP与各指标的相关性。结果:治疗16周后,受试者空腹血糖(FBG)、餐后2 h血糖(2hBG)、糖化血红蛋白(HbA1C)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、腰围(WC)、体质量指数(BMI)、甘油三酯(TG)、胆固醇(TC)、黄体生成素(LH)、LH/FSH、总睾酮(TT)、hs-CRP、Col IV均较治疗前降低,卵泡刺激素(FSH)较治疗前升高,差异均有统计学意义(P<0.05)。司美格鲁肽治疗后hs-CRP与BMI、WBC、HbA1c、FBG、2hBG、FINS、TG、Col IV正相关(P<0.05)。结论:司美格鲁肽既能改善T2DM合并PCOS患者的血糖、血脂、体重、胰岛素抵抗、性激素,也能改善机体的炎症状态及卵巢纤维化,其可能是T2DM合并PCOS患者的潜在治疗靶点。 展开更多
关键词 司美格鲁肽 2型糖尿病 多囊卵巢综合征 超敏C反应蛋白 iv型胶原蛋白
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Pre-hepatectomy type Ⅳ collagen 7S predicts post-hepatectomy liver failure and recovery 被引量:4
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作者 Masatsugu Ishii Osamu Itano +8 位作者 Masahiro Shinoda Minoru Kitago Yuta Abe Taizo Hibi Hiroshi Yagi Ayano Takeuchi Hanako Tsujikawa Tokiya Abe Yuko Kitagawa 《World Journal of Gastroenterology》 SCIE CAS 2020年第7期725-739,共15页
BACKGROUND Liver resection is an effective treatment for benign and malignant liver tumors.However,a method for preoperative evaluation of hepatic reserve has not yet been established.Previously reported assessments o... BACKGROUND Liver resection is an effective treatment for benign and malignant liver tumors.However,a method for preoperative evaluation of hepatic reserve has not yet been established.Previously reported assessments of preoperative hepatic reserve focused only on liver failure in the early postoperative period and did not consider the long-term recovery of hepatic reserve.When determining eligibility for hepatectomy,the underlying pathophysiology needs to be considered to determine if the functional hepatic reserve can withstand both surgery and any postoperative therapy.AIM To identify pre-hepatectomy factors associated with both early postoperative liver failure and long-term postoperative liver function recovery.METHODS This study was a retrospective cohort study.We retrospectively investigated 215 patients who underwent hepatectomy at our hospital between May 2013 and December 2016.Early post-hepatectomy liver failure(PHLF)was defined using the International Study Group of Liver Surgery’s definition of PHLF.Long-term postoperative recovery of liver function was defined as the time taken for serum total bilirubin and albumin levels to return to levels of<2 mg/dL and>2.8 g/dL,respectively,and the time taken for Child-Pugh score to return to Child-Pugh class A.RESULTS Preoperative type IV collagen 7S was identified as a significant independent factor associated with both PHLF and postoperative long-term recovery of liver function.Further analysis revealed that the time taken for the recovery of Child-Pugh scores and serum total bilirubin and albumin levels was significantly shorter in patients with type IV collagen 7S≤6 ng/mL than in those with type IV collagen 7S>6 ng/mL.In additional analyses,similar results were observed in patients without chronic viral hepatitis associated with fibrosis.CONCLUSION Preoperative type IV collagen 7S is a preoperative predictor of PHLF and longterm postoperative liver function recovery.It can also be used in patients without chronic hepatitis virus. 展开更多
关键词 HEPATECTOMY Liver failure Type collagen 7S Liver fibrosis Postoperative complications Long-term postoperative liver function recovery
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Urinary type IV collagen excretion predicts an increased urinary albumin-to-creatinine ratio in normoalbuminuric patients with diabetes 被引量:1
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作者 Susumu Ogawa Masato Matsushima +4 位作者 Masashi Okamura Miho Senda Takuya Sakamoto Kazuhiro Nako Sadayoshi Ito 《Journal of Diabetes Mellitus》 2012年第4期413-426,共14页
Aims: We evaluated whether urinary excretion of type IV collagen (U-COL) may predict an increase in the urinary albumin-to-creatinine ratio (ACR) and what factors regulate U-COL in 145 normoalbuminuric patients with t... Aims: We evaluated whether urinary excretion of type IV collagen (U-COL) may predict an increase in the urinary albumin-to-creatinine ratio (ACR) and what factors regulate U-COL in 145 normoalbuminuric patients with type 2 diabetes. Methods: We measured HbA1c, systolic blood pressure (SBP), urinary 8-hydroxydeoxyguanosine (8-OHdG) and monocyte chemoattractant protein (MCP)-1 at start of this study (Baseline), ACR and U-COL in addition to these measurements at one year later (Evaluation-1), and ACR and SBP after two years of the Evaluation-1 (Evaluation-2). The relationships were investigated between the increase of ACR and the U-COL. The effect of angiotensin receptor blockers (ARB) treatment on the correlations between U-COL and ACR at Evaluation-2 on one hand, and between U-COL and percent change of ACR on the other, was also analyzed. Furthermore, we investigated whether the increase in 8-OHdG and in MCP-1 in a year prior to the Evaluation-1 were risk factors of the rise in U-COL levels. Results: Both U-COL and SBP at Evaluation-1, but not ARB treatment, were independent risk factors for an increased ACR after 2 years. ARB treatment significantly suppressed the increase in ACR after 2 years in patients with higher U-COL excretion. The percentage changes in 8-OHdG (%8-OHdG) and MCP-1 (%MCP-1) in one year prior to Evaluation-1 measurements are independent risk factors for U-COL. HbA1c and SBP values one year prior to Evaluation-1 are independent risk factors not only for %8-OHdG but also, for baseline U-COL. The %8-OHdG is an independent risk factor for %MCP-1. Conclusions: U-COL may predict an increase in the ACR. The U-COL seems to be increased with oxidative stress and inflammation induced by past hyperglycemia. 展开更多
关键词 Type iv collagen Diabetic NEPHROPATHY Oxidative Stress MONOCYTE CHEMOATTRACTANT Protein-1
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IV型胶原蛋白相关听力损失的研究进展
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作者 杨金源 袁永一 《中华耳科学杂志》 CSCD 北大核心 2023年第5期706-710,共5页
IV型胶原蛋白是基底膜等组织的重要组成成分,广泛存在于肾脏、耳蜗等人体器官中。相比引起如肾脏等器官的疾病而言,国内外研究对IV型胶原蛋白相关听力损失的关注相对较少。本文旨在总结已报道的部分IV型胶原蛋白亚型与听力损失相关疾病... IV型胶原蛋白是基底膜等组织的重要组成成分,广泛存在于肾脏、耳蜗等人体器官中。相比引起如肾脏等器官的疾病而言,国内外研究对IV型胶原蛋白相关听力损失的关注相对较少。本文旨在总结已报道的部分IV型胶原蛋白亚型与听力损失相关疾病的研究进展,为后续相关研究提供研究方向参考。 展开更多
关键词 iv型胶原蛋白 ALPORT综合征 听力损失
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Combination of type Ⅳ collagen 7S, albumin concentrations, and platelet count predicts prognosis of non-alcoholic fatty liver disease
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作者 Miwa Kawanaka Ken Nishino +6 位作者 Katsunori Ishii Tomohiro Tanikawa Noriyo Urata Mitsuhiko Suehiro Takako Sasai Ken Haruma Hirofumi Kawamoto 《World Journal of Hepatology》 2021年第5期571-583,共13页
BACKGROUND Non-alcoholic fatty liver disease(NAFLD)is the most common cause of chronic liver disease and affects approximately 25%of the general global adult population.The prognosis of NAFLD patients with advanced li... BACKGROUND Non-alcoholic fatty liver disease(NAFLD)is the most common cause of chronic liver disease and affects approximately 25%of the general global adult population.The prognosis of NAFLD patients with advanced liver fibrosis is known to be poor.It is difficult to assess disease progression in all patients with NAFLD;thus,it is necessary to identify patients who will show poor prognosis.AIM To investigate the efficacy of non-invasive biomarkers for predicting disease progression in patients with NAFLD.METHODS We investigated biomarkers associated with mortality in patients with NAFLD who visited the Kawasaki Medical School General Medical Center from 1996 to 2018 and underwent liver biopsy and had been followed-up for>1 year.Cumulative overall mortality and liver-related events during follow-up were calculated using the Kaplan-Meier analysis and compared using log-rank testing.We calculated the odds ratio and performed receiver operating characteristic curve analysis with logistic regression analysis to determine the optimal cut-off value with the highest prognostic ability.RESULTS We enrolled 489 patients who were followed-up for a period of 1-22.2 years.In total,13 patients died(2.7%of total patients enrolled);7 patients died due to liverrelated causes.Poor prognosis was associated with liver fibrosis on histological examination but not with inflammation or steatosis.Blood biomarkers associated with mortality were platelet counts,albumin levels,and type IV collagen 7S levels.The optimal cutoff index for predicting total mortality was a platelet count of 15×10^(4)/μL,albumin level of 3.5 g/dL,and type IV collagen 7S level of 5 mg/dL.In particular,only one-factor patients with NAFLD presenting with platelet counts≤15×10^(4)/μL,albumin levels≤3.5 g/dL,or type IV collagen 7S≥5 mg/dL showed 5-year,10-year,and 15-year survival rates of 99.7%,98.3%,and 94%,respectively.However,patients with two factors had lower 5-year and 10-year survival rates of 98%and 43%,respectively.Similarly,patients with all three factors showed the lowest 5-year and 10-year survival rates of 53%and 26%,respectively.CONCLUSION A combination of the three non-invasive biomarkers is a useful predictor of NAFLD prognosis and can help identify patients with NAFLD who are at a high risk of all-cause mortality. 展开更多
关键词 Non-alcoholic fatty liver disease Non-alcoholic steatohepatitis Platelet count ALBUMIN Type iv collagen 7S All-cause mortality
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Production and Characterization of Recombinant Rat Non-Collagen Domain of α3 Chain of Type IV Collagen α3 (IV) NC1 Antigen
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作者 Afsana Munni 《CellBio》 2016年第3期27-48,共22页
The glomerulonephritis disease is characterized by inflammation of glomeruli or small blood vessels in the kidney that causes kidney diseases. The reason of glomerulonephritis disease is to deposit the anti-GBM auto a... The glomerulonephritis disease is characterized by inflammation of glomeruli or small blood vessels in the kidney that causes kidney diseases. The reason of glomerulonephritis disease is to deposit the anti-GBM auto antibody in the glomerular basement membrane. The type IV collagen is the main component of glomerular basement membrane that has α3 chain of type (IV) collagen of non-collagenous domain which contains N-terminal 7S domain, a triple helical collagenous domain and C-terminal non-collagenous glomerular domain (NC1). The amino terminal of α3 (IV) NC1 that induces the Experimental Autoimmuno Glomerulonephritis (EAG) in rat model has been identified. The recombinant rat α3 (IV) NC1 antigen has nine amino acid spans that are consistent with antibody or T cell epitope that induces in EAG. The research is carried out on the recombinant rat α3 (IV) NC1 production, purification, quantification, and characterization. The circulation of anti-GBM antibody in glomerular basement membrane can be measured by the ELISA assay. In addition, the recombinant rat antigen is secreted in HEK293 cell supernatant that is purified by Anti-FLAG M2 monoclonal IgG antibody affinity column and characterized and quantified by SDS-PAGE gel electrophoresis and Western blotting techniques. 展开更多
关键词 Auto-Immuno Kidney Disease Glomerulonephritis Disease Glomerular Basement Membrane α3 (iv) NC1-Non-collagen Domain of α3 Chain of Type iv collagen α3 (iv) Antibody(Ab) Antigen (Ag) Anti Glomerular Basement Membrane Experimental Autoimmune Glomerulonephritis Enzyme-Linked Immunosorbent Assay (ELISA) Human Embryonic Kidney (HEK) Ig-Immunoglobulin (IgG IgA) IgAN-IgA nephropathy
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Adsorption of ^(117m)Sn(IV)-EDTMP on hydroxyapatite and collagen
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作者 YANGYu-Qing LUOShun-Zhong 《Nuclear Science and Techniques》 SCIE CAS CSCD 2002年第2期98-104,共7页
Some organic phosphines labeled with ^117mSn(IV) have been proved promising for imaging and pain palliation of bone tumor.In this paper,a preliminary investigation on the adsorption characteristics of EDTMP(ethelenedi... Some organic phosphines labeled with ^117mSn(IV) have been proved promising for imaging and pain palliation of bone tumor.In this paper,a preliminary investigation on the adsorption characteristics of EDTMP(ethelenediaminetetramethylene phosphoric acid)labeled with ^117Sn(IV) on HA( hydroxyapatitie)and collagen,and an investigation on the adsorption mechanism of ^117mSn(IV)-EDTMP on HA was presented. 展开更多
关键词 蛋白 羟基磷灰石 ^117mSn(iv)示踪 吸附
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Changes on lysosomal compartment during PMA-induced differentiation of THP-1 monocytic cells: Influence of type I and type IV collagens
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作者 Alessandra Spano Sergio Barni +1 位作者 Vittorio Bertone Luigi Sciola 《Advances in Bioscience and Biotechnology》 2013年第8期8-18,共11页
In this work, the influence of different substrate adhesion during phorbol-12-myristate-13-acetate (PMA)-induced differentiation of THP-1 monocytic cell line was studied. In particular, by morphocytochemical and cytom... In this work, the influence of different substrate adhesion during phorbol-12-myristate-13-acetate (PMA)-induced differentiation of THP-1 monocytic cell line was studied. In particular, by morphocytochemical and cytometric approaches, the influence of type I and type IV collagens in an experimental model representative of three phases (initial, intermediate and terminal) of monocyte-macrophage transition was analyzed. The cells in these three phases of differentiation were obtained by using 6, 30 e 60 nM PMA. In this experimental model, referring to adhesion to glass as control, by using the azo-dye coupling method, we have considered the analysis of Acid Phosphatase (AcP) activity as a marker of differentiated status expression, in relation to the acquisition of macrophagic phenotype. Endosomal/lysosomal system was further characterized by taking into account the uptake of fluorescent probe LysoTracker Red. Fluorochromization in the various experimental conditions was analyzed morphologically (fluorescence microscopy) and quantitatively (static cytometry). Data related to lysosome compartment were integrated, from a cytokinetic point of view, by flow cytometry measurements of DNA/protein content. Our results have indicated that type I and type IV collagens were able to influence, with respect to glass adhesion, various differentiation phases. Type I collagen showed the higher effects in the condition of high differentiation (60 nM PMA), causing an increase in AcP activity and lysosomal system. Type IV collagen, besides determining effects on lysosomal compartment of intermediate and terminally differentiated cells, influenced mainly proliferative activity of cells with initial differentiation level (6 nM PMA). 展开更多
关键词 THP-1 CELLS Differentiation Type I and iv collagenS Acid PHOSPHATASE LYSOSOMES
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IV型胶原纤维及其酶在胃癌浸润转移中的作用 被引量:19
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作者 薛建元 林言箴 +3 位作者 尹浩然 王瑞年 朱寿柱 朱正纲 《中国肿瘤临床》 CAS CSCD 北大核心 1995年第4期241-243,共3页
采用IV型胶原纤维单抗和IV型胶原酶多抗检测76例胃癌。发现IV型胶原纤维染色所示基底膜明显缺损,在分化较差者及浸润型胃癌中(>75%)明显高于分化较好者及膨胀型胃癌(P<0.005)。IV型胶原酶明显增加在分化较差... 采用IV型胶原纤维单抗和IV型胶原酶多抗检测76例胃癌。发现IV型胶原纤维染色所示基底膜明显缺损,在分化较差者及浸润型胃癌中(>75%)明显高于分化较好者及膨胀型胃癌(P<0.005)。IV型胶原酶明显增加在分化较差者,浸润型及有淋巴结转移者(80%左右)显著高于分化较好者,膨胀型及无淋巴结转移者(P<0.05)。说明分化程度低的胃癌产生更多IV型胶原酶,严重破坏基底膜,促进浸润和淋巴结转移。其可作为恶性程度指标。 展开更多
关键词 胃肿瘤 Ⅳ型胶原纤维 浸润 转移 病理
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High matrix metalloproteinase-9 expression induces angiogenesis and basement membrane degradation in stroke-prone spontaneously hypertensive rats after cerebral infarction 被引量:30
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作者 Huilian Hou Guanjun Zhang +3 位作者 Hongyan Wang Huilin Gong Chunbao Wang Xuebin Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第11期1154-1162,共9页
Basement membrane degradation and blood-brain barrier damage appear after cerebral infarc- tion, severely impacting neuronal and brain functioning; however, the underlying pathogenetic mechanisms remain poorly underst... Basement membrane degradation and blood-brain barrier damage appear after cerebral infarc- tion, severely impacting neuronal and brain functioning; however, the underlying pathogenetic mechanisms remain poorly understood. In this study, we induced cerebral infarction in stroke- prone spontaneously hypertensive rats by intragastric administration of high-sodium water (1.3% NaC1) for 7 consecutive weeks. Immunohistochemical and immunofluorescence assays demonstrated that, compared with the non-infarcted contralateral hemisphere, stroke-prone spontaneously hypertensive rats on normal sodium intake and Wistar-Kyoto rats, matrix metalloproteinase-9 expression, the number of blood vessels with discontinuous collagen IV expression and microvessel density were significantly higher, and the number of continuous collagen IV-positive blood vessels was lower in the infarct border zones of stroke-prone sponta- neously hypertensive rats given high-sodium water. Linear correlation analysis showed matrix metalloproteinase-9 expression was positively correlated with the number of discontinuously collagen IV-labeled blood vessels and microvessel density in cerebral infarcts of stroke-prone spontaneously hypertensive rats. These results suggest that matrix metalloproteinase-9 upregula- tion is associated with increased regional angiogenesis and degradation of collagen IV, the major component of the basal lamina, in stroke-prone spontaneously hypertensive rats with high-sodi- um water-induced focal cerebral infarction. 展开更多
关键词 nerve regeneration cerebral infarction matrix metalloproteinase-9 collagen iv microvessel density ANGIOGENESIS basement membrane degradation high sodium stroke-pronespontaneously hypertensive China Medical Board Project neural regeneration
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层粘连蛋白、纤粘连蛋白、IV型胶原在人月经周期子宫内膜中的表达 被引量:4
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作者 马彩虹 陈贵安 +1 位作者 王海燕 高冬霞 《生殖医学杂志》 CAS 2004年第5期257-261,共5页
目的 研究细胞外基质蛋白层粘连蛋白 (LN)、纤粘连蛋白 (FN)和IV型胶原 (IVCL)在正常人月经周期子宫内膜中的表达。 方法 采用免疫组织化学方法检测 4 9例正常月经周期子宫内膜中LN、FN、IVCL蛋白的表达。 结果 LN主要分布于腺上... 目的 研究细胞外基质蛋白层粘连蛋白 (LN)、纤粘连蛋白 (FN)和IV型胶原 (IVCL)在正常人月经周期子宫内膜中的表达。 方法 采用免疫组织化学方法检测 4 9例正常月经周期子宫内膜中LN、FN、IVCL蛋白的表达。 结果 LN主要分布于腺上皮及腔上皮基底膜 ,分泌中期表达最强。FN主要分布于间质 ,腔上皮及腺上皮基底膜也均有表达 ,分泌中期含量丰富。IVCL在腺上皮基底膜的表达分泌中期最强 ,在间质的表达分泌早期最强 ,分泌中、晚期表达逐渐减弱。LN、FN、IVCL在血管基底膜上均有表达 ,无周期性变化。 结论 LN、FN、IVCL在月经周期子宫内膜中的表达随月经周期而变化。 展开更多
关键词 子宫内膜 蛋白层粘连蛋白 纤粘连蛋白 Ⅳ型胶原
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血管球瘤中FLi-1、h-caldesmon及collagen Ⅳ的表达及临床病理分析 被引量:3
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作者 杨勇 王瑞琳 +1 位作者 徐瑾 尹海波 《临床与实验病理学杂志》 CSCD 北大核心 2017年第12期1337-1341,共5页
目的探讨FLi-1、h-caldesmon及collagenⅣ在血管球瘤中的表达及不同组织学类型与临床病理指标的关系。方法采用免疫组化En Vision法检测35例血管球瘤中FLi-1、h-caldesmon及collagenⅣ的表达。分析35例血管球瘤不同组织学分型与临床病... 目的探讨FLi-1、h-caldesmon及collagenⅣ在血管球瘤中的表达及不同组织学类型与临床病理指标的关系。方法采用免疫组化En Vision法检测35例血管球瘤中FLi-1、h-caldesmon及collagenⅣ的表达。分析35例血管球瘤不同组织学分型与临床病理参数间的关系。结果镜下见肿瘤细胞圆形或多边形,呈片状分布在血管之间或呈环状围绕在血管周围,瘤细胞边界清晰,外形规则,有时可见瘤细胞与梭形平滑肌细胞移行过渡。免疫表型:血管球瘤可表达FLi-1、h-caldesmon及collagenⅣ,阳性率分别为58.6%、97.1%和100%。血管球瘤的不同类型与患者性别和肿瘤体积无关,与患者年龄相关(P=0.01)。35例血管球瘤中vimentin和SMA均呈(+),2例CD34呈局灶(+),1例desmin呈(+),EMA、S-100、Cg A、CD68及CD99均呈(-)。结论 h-caldesmon和collagenⅣ可作为血管球瘤诊断的标志物,FLi-1可作为一种辅助参考标志物,有助于血管球瘤的诊断。 展开更多
关键词 软组织肿瘤 血管球瘤 FLI-1 h-caldesmon collagen 免疫组织化学
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米非司酮对早孕蜕膜组织中IV型胶原及纤维粘连蛋白的影响 被引量:11
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作者 王玉贤 李翘竹 《生殖医学杂志》 CAS 2001年第1期29-33,共5页
目的 :探讨米非司酮 (Ru486 )对早孕蜕膜组织中 IV型胶原、纤维粘连蛋白(FN)的影响 ,进一步认识米非司酮的抗早孕机理。方法 :孕 <6 0 d妇女 ,随机分为对照组和药物组。药物组口服米非司酮总量为 1 5 0 mg。采用免疫组织化学 (ABC)... 目的 :探讨米非司酮 (Ru486 )对早孕蜕膜组织中 IV型胶原、纤维粘连蛋白(FN)的影响 ,进一步认识米非司酮的抗早孕机理。方法 :孕 <6 0 d妇女 ,随机分为对照组和药物组。药物组口服米非司酮总量为 1 5 0 mg。采用免疫组织化学 (ABC)法测定早孕蜕膜组织 IV型胶原 (对照组 1 5例 ,药物组 2 0例 )和纤维粘连蛋白 (对照组 2 5例 ,药物组 30例 )的分布状况。结果 :与对照组相比 ,药物组早孕蜕膜组织中 IV型胶原、FN均明显减少(P<0 .0 1 ,P<0 .0 5 )。尤其是 IV型胶原在腺体基膜 ,FN在蜕膜细胞间质中降低最为明显 (P<0 .0 1 ) ,上皮基膜、血管周围 IV型胶原含量也明显降低 (P<0 .0 5 )。结论 :米非司酮明显影响早孕蜕膜组织中这两种细胞外基质 (ECM)的含量及分布 ,可使 IV型胶原、FN的含量减少 ,而起到抗早孕作用。早孕蜕膜组织中这两种 ECM成分可能受孕激素及糖皮质激素的调控。 展开更多
关键词 米非司酮 iv型胶原 纤维粘连蛋白 蜕膜 抗早孕作用
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Distribution of pericellular matrix molecules in the temporomandibular joint and their chondroprotective effects against inflammation 被引量:6
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作者 Wern Cui Chu Shipin Zhang +5 位作者 Timothy J Sng Yu Jie Ong Wen-Li Tan Vivien Y Ang Casper B Foldager Wei Seong Toh 《International Journal of Oral Science》 SCIE CAS CSCD 2017年第1期43-52,共10页
The objectives of this study were to (1) determine the distribution and synthesis of pericellular matrix (PCM) molecules (collagen VI, collagen IV and laminin) in rat temporomandibular joint (TMJ) and (2) in... The objectives of this study were to (1) determine the distribution and synthesis of pericellular matrix (PCM) molecules (collagen VI, collagen IV and laminin) in rat temporomandibular joint (TMJ) and (2) investigate the effects of PCM molecules on chondrocytes against inflammation in osteoarthritis. Four zones (fibrous, proliferating, mature and hypertrophic) of condylar cartilage and three bands (anterior, intermediate and posterior) of disc were analysed by immunohistochemistry for the presence of PCM molecules in rat TMJs. Isolated chondrocytes were pre-treated with PCM molecules before being subjected to interleukin (IL)-II~ treatment to stimulate inflammation. The responses of the chondrocytes were analysed using gene expression, nitric oxide release and matrix metalloproteinase (MMP)-13 production measures. Histomorphometric analyses revealed that the highest areal deposition of collagen VI (67.4%), collagen IV (45.7%) and laminin (52.4%) was in the proliferating zone of TMJ condylar cartilage. No significant difference in the distribution of PCM molecules was noted among the three bands of the TMJ disc. All three PCM molecules were expressed intracellularly by chondrocytes cultured in the monolayer. Among the PCM molecules, pre-treatment with collagen VI enhanced cellular proliferation, ameliorated IL-lp-induced MMP-3, MMP-9, MMP-13 and inducible nitric oxide synthase gene expression, and attenuated the downregulation of cartilage matrix genes, including collagen I, aggrecan and cartilage oligomeric matrix protein (COMP). Concurrently, collagen VI pretreatment inhibited nitric oxide and MMP-13 production. Our study demonstrates for the first time the distribution and role of PCM molecules, particularly collagen VI, in the protection of chondrocytes against inflammation. 展开更多
关键词 CARTILAGE CHONDROCYTES collagen iv collagen W INFLAMMATION LAMININ pericellular matrix temporomandibular joint
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整合素beta1亚单位对肝癌细胞与IV型胶原黏附与趋化行为的影响 被引量:8
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作者 傅遍红 吴泽志 +1 位作者 董澄 秦建 《生物医学工程学杂志》 EI CAS CSCD 2004年第5期741-745,共5页
采用微吸管实验技术 ,测定肝细胞癌 (Hepatocellular carcinom a,HCC)细胞与 IV型胶原裱衬表面的黏附力 ;进一步加入针对整合素 beta1亚单位 (CD2 9)的单克隆抗体 (Anti- CD2 9)处理 HCC细胞 ,观察 Anti- CD2 9对细胞与 IV型胶原裱衬表... 采用微吸管实验技术 ,测定肝细胞癌 (Hepatocellular carcinom a,HCC)细胞与 IV型胶原裱衬表面的黏附力 ;进一步加入针对整合素 beta1亚单位 (CD2 9)的单克隆抗体 (Anti- CD2 9)处理 HCC细胞 ,观察 Anti- CD2 9对细胞与 IV型胶原裱衬表面的黏附力的影响。采用双微吸管实验法进行 HCC细胞趋化实验 ,在两侧微管内加入相同浓度 (6 0 0μg/ ml )的 IV型胶原 ,并引导微管尖端与同一细胞紧密接触 ,动态观察细胞两侧伪足形成过程 ;在一侧微吸管内加入 2 0μg/ ml Anti- CD2 9,考察 beta1整合素亚单位阻断对 HCC细胞伪足形成的影响。利用流式细胞仪对 HCC细胞表面整合素 beta1亚单位的表达进行分析。结果表明 ,HCC细胞与 5μg/ ml IV型胶原裱衬表面之间的黏附力为 932± 134(× 10 - 1 0 N ,n=6 0 ) ,加入 5μg/ ml Anti- CD2 9黏附力减小到 4 4 9± 119(× 10 - 1 0 N,n=6 0 ) ;加入 10 μg/ ml Anti- CD2 9时黏附力减小到 2 2 0± 78(× 10 - 1 0 N,n=5 5 )。双微吸管趋化实验表明 :两侧微吸管加入相同浓度 IV型胶原 ,细胞向两侧微吸管均有伪足形成 ;在此基础上 ,微吸管加入 Anti- CD2 9的一侧 ,HCC细胞伪足生长曲线呈现明显的抑制 ,而未加入 Anti- CD2 9的微吸管一侧与加入的对侧相比 ,该侧细胞的伪足明显增? 展开更多
关键词 iv型胶原 HCC CD29 细胞 整合素 亚单位 黏附力 微管 生长曲线 表达率
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140例Graves’病患者血清HA、LN、PCⅢ、IV.C含量的临床分析 被引量:5
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作者 程刚 罗加 +1 位作者 邓红 吴红燕 《重庆医科大学学报》 CAS CSCD 2004年第3期381-382,388,共3页
目的 :应用血清中的HA、LN、PCⅢ、IV .C含量 ,来判断甲状腺功能亢进对肝脏功能的影响。方法 :采用放射免疫法测定 14 0例Graves’病患者和 33例健康体检者的血清HA、LN、PCⅢ、IV .C含量。Graves’病患者分为初诊未用ATD组 ( 5 9例 )... 目的 :应用血清中的HA、LN、PCⅢ、IV .C含量 ,来判断甲状腺功能亢进对肝脏功能的影响。方法 :采用放射免疫法测定 14 0例Graves’病患者和 33例健康体检者的血清HA、LN、PCⅢ、IV .C含量。Graves’病患者分为初诊未用ATD组 ( 5 9例 )和曾用ATD组 ( 81例 )。结果 :血清HA含量未用ATD组 (均数±标准差 119.4 3± 10 7.0 8μg/L)明显高于对照组 ( 78.30±5 8.4 4 μg/L) ,P <0 .0 5 ,而曾用ATD组 ( 10 8.4 9± 6 6 .94 μg/L)与对照组之间无显著性差异 ;血清LN、PCⅢ、IV .C含量在所有的病人均显著高于对照组 ,P <0 .0 0 1,未用ATD组分别为 ( 2 0 3.18± 98.5 5 )、( 2 0 9.0 6± 10 8.6 2 )、( 117.79± 6 3.39) μg/L ,曾用ATD组分别为 ( 190 .0 3± 79.37)、( 172 .36± 10 4 .4 5 )、( 119.0 2± 4 7.38) μg/L ,对照组分别为 ( 130 .88± 15 .12 )、( 74 .5 9± 2 1.87)、( 6 4 .5 6± 2 7.6 4 ) μg/l。 结论 :Graves’病患者无论是否接受过ATD治疗 ,血清LN、PCⅢ、IV .C增高 ;HA在初诊病人升高 ,而接受过ATD治疗的病人正常 ,也许与ATD的免疫调节有关。 展开更多
关键词 Graves’病 透明质酸 层粘蛋白 Ⅲ型前胶原肽 iv型胶原
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癌基因蛋白P^(21)、P^(185)、EGFR及LN、IV型胶原在骨肉瘤中的表达 被引量:2
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作者 宝建中 郑建明 +2 位作者 周霖 倪灿荣 张春才 《肿瘤防治研究》 CAS CSCD 北大核心 1999年第4期265-267,共3页
目的探讨癌基因蛋白p21、p185、EGFR及LN、IV型胶原在人骨肉瘤组织中表达的意义。方法应用免疫组化ABC法,检测40例常规石蜡包埋的骨肉瘤组织中p21、p185和EGFR的表达,同时还检测了LN、IV型胶原在... 目的探讨癌基因蛋白p21、p185、EGFR及LN、IV型胶原在人骨肉瘤组织中表达的意义。方法应用免疫组化ABC法,检测40例常规石蜡包埋的骨肉瘤组织中p21、p185和EGFR的表达,同时还检测了LN、IV型胶原在骨肉瘤组织中的含量。结果p21、p185和EGFR在骨肉瘤组织中的阳性表达率分别为263%(10/38),658%(25/38),500(19/38),同时观察到LN和IV型胶原在骨肉瘤组织中往往是缺如的。结论骨肉瘤组织中存在着多种癌基因的异常,它们与骨肉瘤的发生发展密切相关,同时细胞外基质的破坏在骨肉瘤的侵袭与转移中起着重要的作用。 展开更多
关键词 骨肉瘤 ABC法 癌基因蛋白 LN iv型胶原 骨肿瘤
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带蒂大网膜包肾术对肾小球硬化大鼠肾皮质IV型胶原基因表达的影响 被引量:3
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作者 董兴刚 安增梅 +3 位作者 杨海春 周江华 殷祥雷 顾建新 《中国血液净化》 2004年第4期205-206,共2页
目的 探讨带蒂大网膜包肾术 (POT)对肾小球硬化大鼠肾皮质IV型胶原基因表达的影响。方法采用单侧肾切除加二次注射阿霉素制备肾小球硬化大鼠模型 ,观察带蒂大网膜包肾术对肾小球硬化大鼠肾皮质IV型胶原基因表达的影响。结果 带蒂大网... 目的 探讨带蒂大网膜包肾术 (POT)对肾小球硬化大鼠肾皮质IV型胶原基因表达的影响。方法采用单侧肾切除加二次注射阿霉素制备肾小球硬化大鼠模型 ,观察带蒂大网膜包肾术对肾小球硬化大鼠肾皮质IV型胶原基因表达的影响。结果 带蒂大网膜包肾术使肾小球硬化大鼠肾皮质IV型胶原基因表达明显上调 ,肾小球硬化减轻。结论 带蒂大网膜包肾术下调肾小球硬化大鼠肾皮质IV型胶原基因表达 。 展开更多
关键词 带蒂大网膜包肾术 肾小球硬化 大鼠 POT 肾皮质 iv型胶原 基因表达 肾组织
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血清透明质酸、层粘连蛋白及IV型胶原在肝纤维化及肝癌中的变化分析 被引量:2
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作者 邓芝云 张方信 +2 位作者 于兰 高福兴 吕同德 《西北国防医学杂志》 CAS 2001年第1期60-62,共3页
目的 :研究血清透明质酸 (HA)、层粘连蛋白 (LN)及IV型胶原 (IV -C)水平与肝纤维化及肝癌转移的关系。方法 :应用放射免疫法测定 6 5例慢性病毒性肝炎、10 8例肝炎后肝硬化及 5 1例原发性肝癌患者血清HA、LN及IV -C水平。结果 :肝癌、... 目的 :研究血清透明质酸 (HA)、层粘连蛋白 (LN)及IV型胶原 (IV -C)水平与肝纤维化及肝癌转移的关系。方法 :应用放射免疫法测定 6 5例慢性病毒性肝炎、10 8例肝炎后肝硬化及 5 1例原发性肝癌患者血清HA、LN及IV -C水平。结果 :肝癌、肝硬化及慢性肝炎患者的血清三项指标均明显高于对照者 ,肝硬化患者的上述指标还明显高于慢性肝炎者 ,其敏感性 (84.9% )及准确性 (79.8% )以HA最高 ,而特异性 (96 .9% )则以三项指标联检最佳。此外 ,伴肝外转移的血清LN及IV -C含量还显著高于无肝外转移者。结论 :血清三项指标基本与慢性肝炎及肝硬化的发展过程相一致 ,可作为反映肝纤维化程度及监测肝癌浸润转移的临床指标。 展开更多
关键词 肝纤维化 肝癌 透明质酸 Ⅳ型胶原 层粘连蛋白
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水蛭提取液对体外培养RF/6A的细胞液中MMP-2、IV型胶原含量的影响 被引量:2
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作者 郑燕林 刘聪慧 沙菽 《眼科新进展》 CAS 北大核心 2014年第1期13-16,共4页
目的初探水蛭提取液对新生血管生长的作用机制。方法采用酶联免疫法、放射免疫法检测不同时间点水蛭提取液对细胞培养上清液中MMP-2、IV型胶原表达的影响。结果 12 h各组间比较,空白组、水蛭组MMP-2的OD值分别为0.137±0.006、0.136... 目的初探水蛭提取液对新生血管生长的作用机制。方法采用酶联免疫法、放射免疫法检测不同时间点水蛭提取液对细胞培养上清液中MMP-2、IV型胶原表达的影响。结果 12 h各组间比较,空白组、水蛭组MMP-2的OD值分别为0.137±0.006、0.136±0.007,表达低于凝血酶组0.165±0.018,组间差异均有统计学意义(均为P<0.05)。空白组12 h的MMP-2含量要低于2 h、6 h,差异均有统计学意义(均为P<0.05);水蛭组2 h时MMP-2的分泌高于6 h、12 h,差异均有统计学意义(均为P<0.05)。2 h时空白组IV型胶原的含量(20.67±1.02)ng·mL-1高于凝血酶组(15.58±3.40)ng·mL-1、合药组(15.75±6.23)ng·mL-1,差异均有统计学意义(均为P<0.05);6 h时水蛭组IV型胶原的含量明显高于其他3组,差异均有统计学意义(均为P<0.05);12 h时观察,空白组IV型胶原的含量(21.33±2.75)ng·mL-1,明显高于合药组(16.49±1.59)ng·mL-1,差异有统计学意义(P<0.05),水蛭组IV型胶原的含量明显高于凝血酶组及合药组,差异有统计学意义(P<0.05)。空白组2 h、12 h IV型胶原的含量均较6 h高,差异均有统计学意义(均为P<0.05);凝血酶组IV型胶原的合成呈增加趋势,12 h与2 h相比,差异有统计学意义(P<0.05);水蛭组IV型胶原的合成明显增加,6 h、12 h IV型胶原的含量显著高于2 h,差异有统计学意义(P<0.05)。结论64 g·L-1水蛭提取液可以促进IV型胶原合成,抑制MMP-2的分泌,对新生血管的形成有抑制作用。 展开更多
关键词 水蛭提取液 基质金属蛋白酶-2 iv型胶原
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