EFFECT OF NITROGEN AND PHOSPHORUS ON THE GROWTH OF A RED TIDE DINOFLAGELLATE SCRIPPSIELLA TROCHOIDEA (STEIN) LOEBLICH III

Scrippsiella trochoidea (Stein) Loeblich III was grown in a nitrogen or phosphorus limited batch culture system in laboratory. Growth rates and cellular Chl a were measured as functions of nitrate and phosphate concentrations. Growth rates were hyperbolic with both nitrate and phosphate concentration and fit the Monod equation. The minimum cell quota of nitrogen and phosphorus and then the optimum N:P ratio of S. trochoidea were estimated in this study. Measurement of phosphate concentration in Jiaozhou Bay suggest that phosphorus is the limiting factor of S. trochoidea growth.

Yield performance and optimal nitrogen and phosphorus application rates in wheat and faba bean intercropping

Yield performance in cereal and legume intercropping is related to nutrient management,however,the yield response of companion crops to nitrogen(N)input is inconclusive and only limited efforts have focused on rationed phosphorous(P)fertilization.In this study,two multi-year field experiments were implemented from 2014-2019 under identical conditions.Two factors in a randomized complete block design were adopted in both experiments.In field experiment 1,the two factors included three planting patterns(mono-cropped wheat(MW),mono-cropped faba bean(MF),and wheat and faba bean intercropping(W//F))and four N application rates(N0,0 kg N ha^(-1);N1,90 and 45 kg N ha^(-1) for wheat and faba beans,respectively;N2,180 and 90 kg N ha^(-1) for wheat and faba beans,respectively;and N3,270 and 135 kg N ha^(-1) for wheat and faba beans,respectively).In field experiment 2,the two factors included three P application rates(P0,0 kg P_(2)O_(5) ha^(-1);P1,45 kg P_(2)O_(5) ha^(-1);and P2,90 kg P_(2)O_(5) ha^(-1))and the same three planting patterns(MW,MF,and W//F).The yield performances of inter-and mono-cropped wheat and faba beans under different N and P application rates were analyzed and the optimal N and P rates for intercropped wheat(IW)and MW were estimated.The results revealed that intercropping favored wheat yield and was adverse to faba bean yield.Wheat yield increased by 18-26%,but faba bean yield decreased by 5-21% in W//F compared to MW and MF,respectively.The stimulated IW yield drove the yield advantage in W//F with an average land equivalent ratio(LER)of 1.12.N and P fertilization benefited IW yield,but reduced intercropped faba bean(IF)yield.Nevertheless,the partial LER of wheat(pLER_(wheat))decreased with increasing N application rates,and the partial LER of faba bean(pLER_(faba bean))decreased with increasing P application rates.Thus,LER decreased as N input increased and tended to decline as P rates increased.IW maintained a similar yield as MW,even under reduced 40-50% N fertilizer and 30-40% P fertilizer conditions.The estimated optimum N application rates for IW and MW were 150 and 168 kg ha^(-1),respectively,and 63 and 62 kg ha^(-1) for P_(2)O_(5),respectively.In conclusion,W//F exhibited yield advantages due to stimulated IW yield,but the intercropping yield benefit decreased as N and P inputs increased.Thus,it was concluded that modulated N and P rates could maximize the economic and ecological functions of intercropping.Based on the results,rates of 150 kg N ha^(-1) and 60 kg P_(2)O_(5) ha^(-1) are recommended for IW production in southwestern China and places with similar conditions.

Influence of the Macronutrients N, P and K on the Agarophyte <i>Alsidium triquetrum</i>(S. G. Gmelin) Trevisan, during Experimental Culture

The knowledge of the nutritional requirements and their relation to the physiology of marine algae growth is key to incorporate new species into aquaculture, whose dynamics tend to be largely unknown. The use of Alsidium triquetrum in the pharmacological industry depends on its availability in the natural environment, occasionally scarce. As macroalgae cultivation gains momentum worldwide, it is important to know how the effects of nutrients are modulated in the thallus during cultivation. The linking of the relative growth rates (RGR) of A. triquetrum and their relation with the macronutrients N (nitrogen), P (phosphorus) and K (potassium) at the tissue level under culture conditions constitutes the main contribution of this article. P levels tend to decrease as the plant completes its development. Both the concentration of N and P are higher in the stipe for the month of July, N (25.31 ± 0.26) vs P (0.846 ± 0.02) period when the highest vegetative development is reached. The N and P modulate the patterns of the species’ development over the annual cycle, unlike K, which is not considered a limiting factor. When the temperature and lighting are not favorable for growth, the plant simply accumulates these compounds. As environmental conditions change, these stored compounds are actively used in their growth. The specimens with an initial weight of 50 g present the best accumulated biomass (RGR) throughout the annual cycle.

Discussion on the Operation of Urban Domestic Sewage Treatment Facilities: A Case Study of Guangdong Province

Urban sewage treatment facility is an important ways to control polluted domestic water. The operation of urban sewage treatment facilities is good or not, and whether the effectiveness of pollutant emission reduction can be exerted play a significant role in reducing the pollution of living sources. Nowadays the operation of urban sewage treatment facilities is mainly evaluated by the load rate. However, due to the failure to fully implement the reformation of the rainwater and polluted water in Guangdong Province, the domestic sewage is mixed with rainwater during the rainy season and is included in the treatment of urban sewage treatment facilities. Therefore, it is not objective to evaluate the operation of sewage treatment facilities using only the load rate of sewage treatment. According to the situation of Guangdong Province, the load rates of COD, ammonia nitrogen and total phosphorus in domestic sewage and load rate of sewage treatment were included in the evaluation, and 20 urban sewage treatment facilities were selected as research objects. The operation situations and emission reduction benefits of urban sewage treatment facilities in different regions of Guangdong Province were roughly evaluated.

基于文献分析的氮肥用量对小麦玉米轮作体系硝态氮淋溶的影响

氮淋溶是氮素损失的主要途径之一,土壤硝态氮通过淋溶进入水体会造成人体中毒和水体富营养化,对人体健康和生态环境产生严重危害。为探明我国冬小麦-夏玉米轮作体系化肥施氮量、作物种类和监测方法等因素对硝态氮淋溶量的影响,利用中国知网期刊全文数据库(CNKI)和Web of Science核心合集英文数据库(WoS),收集1980—2020年冬小麦-夏玉米轮作体系硝态氮淋溶领域的相关文献,采用回归方程和T检验等统计学方法分析不同施氮水平、作物种类和监测方法对冬小麦-夏玉米轮作体系硝态氮淋溶的影响。结果表明,随着化肥施氮量的增加,小麦季和玉米季硝态氮淋溶量均呈指数增长趋势。小麦季和玉米季平均施氮量差异不显著,但小麦季硝态氮淋溶量和淋溶率显著低于玉米季(P<0.01)。在施氮量接近的条件下,采用渗漏计法和溶液提取器法测得的硝态氮淋溶量和淋溶率没有显著差异,两种方法均可作为监测硝态氮淋溶的可靠方法在田间应用。回归方程对施氮量和硝态氮淋溶量的拟合在施氮量低于300 kg∙hm^(−2)时较好,随着施氮量的增加拟合精确度降低。玉米季硝态氮淋溶风险大于小麦季,冬小麦-夏玉米轮作体系中玉米季的硝态氮淋溶现象需要引起重视。

大薸与满江红对网箱养殖长吻鮠氮磷排放的影响

为缓解网箱养鱼对承载水体造成的污染,以漂浮植物大藻(Pistia stratiotes L.)和满江红[Azolla imbircata(Roxb.)Nakai]为研究对象,设置满江红、大藻及混养三组生态网箱以及传统网箱,探究种植水生植物对网箱养殖长吻(?)氮磷排放的影响。结果表明:三组生态网箱(满江红、大藻、混养)和传统网箱氮的回收率分别为38.43%、36.20%、36.14%和34.18%,磷的回收率分别为30.09%、29.01%、28.18%和27.00%,三组生态网箱的氮磷回收率显著高于传统网箱,而大藻生态网箱的氮磷回收率与混养生态网箱无显著性差异,满江红生态网箱的氮回收率与大藻及混养生态网箱均有显著性差异,但三组生态网箱间磷的回收率之间无显著性差异。四组网箱之间的氮磷利用率无显著性差异。通过比较,满江红对氮磷移除效果优于大藻和混养,当网箱面积与满江红种植面积为1:24.64左右时,养殖长吻(?)网箱的氮和磷为零排放。

Effect of the C:N:P ratio on the denitrifying dephosphatation in a sequencing batch biofilm reactor(SBBR)

A series of investigations were conducted using sequencing batch biofilm reactor（SBBR） to explore the influence of C：N：P ratio on biological dephosphatation including the denitrifying dephosphatation and the denitrification process.Biomass in the reactor occurred mainly in the form of a biofilm attached to completely submerged disks.Acetic acid was used as the source of organic carbon.C：N：P ratios have had a significant effect on the profiles of phosphate release and phosphate uptake and nitrogen removal.The highest rates of phosphate release and phosphate uptake were recorded at the C：N：P ratio of 140：70：7.The C：N ratio of 2.5：1 ensured complete denitrification.The highest rate of denitrification was achieved at the C：N：P ratio of 140：35：7.The increase of nitrogen load caused an increase in phosphates removal until a ratio C：N：P of 140：140：7.Bacteria of the biofilm exposed to alternate conditions of mixing and aeration exhibited enhanced intracellular accumulation of polyphosphates.Also,the structure of the biofilm encouraged anaerobic-aerobic as well as anoxic-anaerobic and absolutely anaerobic conditions in a SBBR.These heterogeneous conditions in the presence of nitrates may be a significant factor determining the promotion of denitrifying polyphosphate accumulating organism（DNPAO） development.