期刊文献+
共找到600篇文章
< 1 2 30 >
每页显示 20 50 100
臭椿炭疽病菌盘长孢刺盘孢(Colletotrichum gloeosporioides Penz.)生物学特性研究 被引量:3
1
作者 王教敏 付卫东 +3 位作者 吴云 张国良 杨明丽 Richard Reardon 《山东农业大学学报(自然科学版)》 CSCD 北大核心 2009年第1期27-31,共5页
本研究测定了不同培养基、碳源、氮源、温度、pH条件下臭椿盘长孢刺盘孢(Colletotrichum gloeosporioides Penz.)菌株SQD-107的生长、产孢和孢子萌发情况。该菌株在PSA培养基上生长最快、产孢最多;在供测碳源中病原菌对蔗糖、葡萄糖利... 本研究测定了不同培养基、碳源、氮源、温度、pH条件下臭椿盘长孢刺盘孢(Colletotrichum gloeosporioides Penz.)菌株SQD-107的生长、产孢和孢子萌发情况。该菌株在PSA培养基上生长最快、产孢最多;在供测碳源中病原菌对蔗糖、葡萄糖利用较好,供测氮源中对硝酸铵、氯化铵利用较好。菌丝生长的适宜温度范围为10~35℃,最适25℃;产生分生孢子的适宜温度范围为10~30℃,最适25~30℃;孢子萌发的适宜温度范围为5~35℃,最适25℃。在pH3~10的范围内该菌均能生长和产孢,菌丝生长最适pH7~8;产生分生孢子最适pH为7,孢子萌发最适pH6~8。孢子的致死温度为60℃,持续时间10min。 展开更多
关键词 臭椿盘长孢刺盘孢 培养条件 菌丝生长 产孢 孢子萌发
下载PDF
芒果炭疽病[Colletotrichum gloeosporioides(Penz)]生物学及其综合防治研究 被引量:23
2
作者 周又生 沈发荣 赵焕萍 《西南农业大学学报(自然科学版)》 CSCD 1996年第3期206-209,共4页
在云南景谷芒果种植区,芒果炭疽病对芒果树嫩叶、嫩梢、花穗和果实为害率分别为85.7%,63.4%,77.1%和73.3%。风雨和昆虫媒介是传播的主要方式。发生为害最适温度为22℃~25℃,相对湿度为85%~100%。... 在云南景谷芒果种植区,芒果炭疽病对芒果树嫩叶、嫩梢、花穗和果实为害率分别为85.7%,63.4%,77.1%和73.3%。风雨和昆虫媒介是传播的主要方式。发生为害最适温度为22℃~25℃,相对湿度为85%~100%。该病在景谷种植区全年均可发生为害,主要为害在发生期。发生前期为害,是花穗干瘪和幼果掉落的原因之一。采取综合防治措施,特别在发生前期适时防治,可控制发生为害。 展开更多
关键词 芒果 炭疽病 发生规律 综合防治
下载PDF
Resistance risk and molecular mechanism associated with resistance to picoxystrobin in Colletotrichum truncatum and Colletotrichum gloeosporioides 被引量:1
3
作者 SHI Niu-niu LIAN Jin-pan +2 位作者 QIU De-zhu CHEN Fu-ru DU Yi-xin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第12期3681-3693,共13页
Anthracnose,caused by Colletotrichum truncatum and C.gloeosporioides,is amongst the most serious diseases of soybean in China.Picoxystrobin,a quinone outside inhibitor fungicide,is commonly used for the control of ant... Anthracnose,caused by Colletotrichum truncatum and C.gloeosporioides,is amongst the most serious diseases of soybean in China.Picoxystrobin,a quinone outside inhibitor fungicide,is commonly used for the control of anthracnose.Its resistance risk and mechanism in C.truncatum and C.gloeosporioides are unclear.In this study,the sensitivities of 128 C.truncatum and 121 C.gloeosporioides isolates to picoxystrobin were investigated,and unimodal distributions were observed with average EC_(50)values of 0.7740 and 1.1561μg mL^(-1),respectively.Eleven picoxystrobin-resistant mutants of C.truncatum and six mutants of C.gloeosporioides were acquired,with EC_(50)values varying from 5.40-152.96 and 13.53-28.30μg mL^(-1),respectively.Compared to the parental isolates,mutants showed similar or higher relative fitness in conidial production and germination,and pathogenicity.Collectively,the resistance risk of C.truncatum and C.gloeosporioides to picoxystrobin is moderate to high.There was positive cross-resistance between picoxystrobin and pyraclostrobin,but not between picoxystrobin and fluazinam,difenoconazole,or propiconazole.The G143S mutation in Cyt b protein was detected in seven high-resistant mutants of C.truncatum(RF>100),and G137R occurred in four moderate-resistant mutants(RF<_(50)).Contrastingly,there were no point mutations in Cyt b of any C.gloeosporioides mutants.Molecular docking confirmed that two mutations conferred different resistance levels to picoxystrobin.Under greenhouse trials,picoxystrobin did not control mutants with the G143S mutation,those bearing G137R or no point mutation were somewhat controlled,but at a lower level compared to wild-type isolates.These results showed that integrated management strategies should be implemented to preserve fungicide effectiveness. 展开更多
关键词 colletotrichum truncatum colletotrichum gloeosporioides picoxystrobin point mutation Cyt b molecular docking
下载PDF
芒果炭疽病菌,Colletotrichum gloeosporioides的生物学特性 被引量:29
4
作者 黄思良 霍秀娟 韦刚 《西南农业学报》 CSCD 北大核心 1999年第2期83-89,共7页
芒果炭疽病菌Coletotrichumgloeosporioides的营养生长、孢子形成、孢子萌发研究表明,营养生长和孢子形成的温度范围均为7~37℃,适温范围分别为20~31℃,25~31℃。可以生长的pH值范围很... 芒果炭疽病菌Coletotrichumgloeosporioides的营养生长、孢子形成、孢子萌发研究表明,营养生长和孢子形成的温度范围均为7~37℃,适温范围分别为20~31℃,25~31℃。可以生长的pH值范围很广,以pH5.0~8.0生长良好;产孢的适宜pH为3.5~4.5。红光,兰光抑制营养生长,但光照有利于产孢,以萤光的促进作用最强。分生孢子在10~40℃均可萌发,适温范围为25~31℃。附着胞形成的温度范围为15~35℃,最适为30℃。在相对湿度100%+水膜的条件下分生孢子萌发良好。营养有利于分生孢子萌发和附着胞形成。分生孢子萌发的pH为2~11,最适pH为3~7,附着胞形成的最适pH值为3~6。 展开更多
关键词 芒果 炭疽病菌 生物学特性
下载PDF
蓝光诱导的胶孢炭疽菌(Colletotrichum gloeosporioides)类胡萝卜素积累 被引量:6
5
作者 付鸣佳 王小菁 《微生物学报》 CAS CSCD 北大核心 2005年第5期795-797,共3页
胶孢炭疽菌(Colletotrichumgloeosporioides)为一种丝状真菌,蓝光照射可诱导类胡萝卜素的积累。光镜下观察表明,蓝光可诱导胶孢炭疽菌菌丝积累色素颗粒,而黑暗和红光处理却无此现象。类胡萝卜素的积累受蓝光光照强度的影响。28℃且蓝光... 胶孢炭疽菌(Colletotrichumgloeosporioides)为一种丝状真菌,蓝光照射可诱导类胡萝卜素的积累。光镜下观察表明,蓝光可诱导胶孢炭疽菌菌丝积累色素颗粒,而黑暗和红光处理却无此现象。类胡萝卜素的积累受蓝光光照强度的影响。28℃且蓝光为6.5μmol.m-2.s-1时,类胡萝卜素积累量可随光照时间延长呈增长趋势,在第5天达到最高峰为71.8μg/g FW,随后含量下降。此外,胶孢炭疽菌在黑暗中预培养的时间也影响蓝光的诱导反应。 展开更多
关键词 胶孢炭疽菌 蓝光 类胡萝卜素
下载PDF
Isolation and Identification of Colletotrichum gloeosporioides in Pears and Its Biological Characteristics 被引量:1
6
作者 刘邮洲 陈志谊 +3 位作者 钱国良 聂亚峰 乔俊卿 刘凤权 《Agricultural Science & Technology》 CAS 2014年第2期191-195,共5页
[Objective] This study aimed to explore the biological characteristics of Col etotrichum gloeosporioides in pears. [Method] Twenty-five C. gloeosporioides strains were isolated and identified from the diseased samples... [Objective] This study aimed to explore the biological characteristics of Col etotrichum gloeosporioides in pears. [Method] Twenty-five C. gloeosporioides strains were isolated and identified from the diseased samples. Their pathogenicity was identified by inoculating the surface of punctured pears with fungal discs. The effects of different temperatures, pH values, carbon sources and nitrogen sources on the growth of C. gloeosporioides mycelia were explored by incubating fungal discs on the center of plates. [Result] Among the twenty-five C. gloeosporioides strains, three had strong pathogenicity, and eighteen had intermediate pathogenicity, and four strains had weak pathogenicity. Those highly-pathogenic strains had darker colonies, with dense mycelia, whereas those lowly-pathogenic ones had white colonies, with sparse mycelia. Those with fast-growing colonies showed strong pathogenicity, while those with slowly-growing colonies displayed weak pathogenicity. There was no relationship between conidia yield and pathogenicity. The optimum temperature for the growth of C. gloeosporioides mycelia was 25-30 ℃, and the optimum pH was 5.0-7.0. C. gloeosporioides could make use of various carbon sources (monosaccharide and disaccharide), inorganic and organic nitrogen sources, and the optimal carbon source and nitrogen source were sucrose and beef extract, respectively. [Conclusion] Our study benefits further understanding of C. gloeospori-oides and helps to control pear anthracnose more effectively. 展开更多
关键词 colletotrichum gloeosporioides PATHOGENICITY Biological characteristics
下载PDF
柑桔叶面炭疽病Colletotrichum gloeosporioides附着胞的研究 被引量:1
7
作者 王忠肃 华世珍 欧阳秩 《西南农业大学学报(自然科学版)》 CSCD 1991年第1期103-105,共3页
在自然条件及人工接种外观无症柑桔叶片表明,柑桔叶片表面存在大量炭疽病菌的附着胞。人工接种幼苗的叶片上虽有大量附着胞存在,但经23个月后仍未见显症发病现象。接种植株上的新叶未见病菌附着胞。叶片组织解剖未发现病菌菌丝,这说明... 在自然条件及人工接种外观无症柑桔叶片表明,柑桔叶片表面存在大量炭疽病菌的附着胞。人工接种幼苗的叶片上虽有大量附着胞存在,但经23个月后仍未见显症发病现象。接种植株上的新叶未见病菌附着胞。叶片组织解剖未发现病菌菌丝,这说明柑桔炭疽病潜伏侵染结构是普遍存在于柑桔叶面的附着胞。 展开更多
关键词 柑桔 叶面 炭疽病 附着孢
下载PDF
辣椒炭疽病菌Colletotrichum gloeosporioides对啶氧菌酯的敏感基线及抗性突变体生物学性状 被引量:12
8
作者 任璐 周建波 +3 位作者 刘慧平 曹俊宇 殷辉 赵晓军 《植物保护》 CAS CSCD 北大核心 2017年第6期29-37,共9页
为评估辣椒炭疽病菌Colletotrichum gloeosporioides对啶氧菌酯的抗性风险,建立了辣椒炭疽病菌对啶氧菌酯的敏感基线,室内筛选获得了辣椒炭疽病菌抗啶氧菌酯突变体,并对突变体生物学性状进行了研究。在山西省3个未使用过啶氧菌酯及QoI... 为评估辣椒炭疽病菌Colletotrichum gloeosporioides对啶氧菌酯的抗性风险,建立了辣椒炭疽病菌对啶氧菌酯的敏感基线,室内筛选获得了辣椒炭疽病菌抗啶氧菌酯突变体,并对突变体生物学性状进行了研究。在山西省3个未使用过啶氧菌酯及QoI类药剂的地区采集并分离到45株辣椒炭疽病菌。采用菌丝生长速率法测定其对啶氧菌酯的敏感性,结果显示,EC_(50)值介于1.404~16.650μg/mL,平均EC_(50)值为(6.783±3.499)μg/mL。啶氧菌酯与水杨肟酸(SHAM)共同处理时(SHAM的处理浓度为100μg/mL),EC_(50+S)为0.022~0.275μg/mL,平均(0.109±0.058)μg/mL,EC_(50+S)呈连续性单峰曲线,且敏感性频率分布呈近似正态分布,EC_(50+S)平均值可作为辣椒炭疽病菌对啶氧菌酯的敏感基线。且水杨羟肟酸旁路氧化贡献值F(F=EC_(50)/EC_(50+S))最低为6.039,最高为301.441,平均78.026。室内诱导获得8株抗性突变体,其中低抗突变体6株,中抗突变体2株。中抗突变体无性繁殖10代,其抗性可稳定遗传。突变体产孢量和菌丝生长速率与敏感菌株相比均无显著差异,但无药条件下,突变体致病力较敏感菌株有所降低。抗感菌株均以淀粉作为碳源、硝酸钾作为氮源时利用率最高;最适pH均为5和6;菌丝的最适生长温度均为25℃。表明辣椒炭疽病菌抗啶氧菌酯突变体具有较高的适合度,对啶氧菌酯具有较高抗性风险,这一研究结论为指导生产用药,延缓抗药性发展提供理论依据。 展开更多
关键词 辣椒炭疽病菌 啶氧菌酯 敏感基线 抗性风险 生物学性状
下载PDF
柑桔炭疽病菌Colletotrichum gloeosporioides抗药力试验 被引量:6
9
作者 华世珍 王忠肃 欧阳秩 《西南农业大学学报(自然科学版)》 CSCD 1991年第1期96-98,共3页
常用杀菌剂对柑桔炭疽病菌分生孢子杀伤力高达100%,可以阻止它的萌发,但对叶面潜存的附着胞效果很差。附着胞只能在植株器官组织衰弱条件下才能萌发侵入。因此,改善栽培管理条件、增强树势、提高抗病力就能有效地控制炭疽病的发生。
关键词 柑桔 炭疽病菌 潜伏侵染 带菌部位
下载PDF
枸杞内生真菌对胶孢炭疽菌Colletotrichum gloeosporioides的拮抗作用及生防潜力 被引量:19
10
作者 胡丽杰 闫思远 +2 位作者 李嘉泓 杜娟 顾沛雯 《植物保护》 CAS CSCD 北大核心 2020年第1期125-133,共9页
为了明确枸杞内生真菌对胶孢炭疽菌的抑制作用,通过室内抑菌试验和离体试验测定了内生真菌对胶孢炭疽菌的抑制作用及对枸杞炭疽病的防治效果。结果表明,镰刀菌属Fusarium菌株NQ8GII4、NQ8GII7、NQ7GII4和篮状菌属Talaromyces菌株NQ6GII... 为了明确枸杞内生真菌对胶孢炭疽菌的抑制作用,通过室内抑菌试验和离体试验测定了内生真菌对胶孢炭疽菌的抑制作用及对枸杞炭疽病的防治效果。结果表明,镰刀菌属Fusarium菌株NQ8GII4、NQ8GII7、NQ7GII4和篮状菌属Talaromyces菌株NQ6GIII11对胶孢炭疽菌有明显的拮抗作用。平板对峙试验显示,上述4株枸杞内生真菌具有较强的营养和空间竞争能力,菌株NQ8GII4对病原菌的抑制率高达93.43%。显微观察发现,枸杞内生真菌能缠绕并穿透胶孢炭疽菌菌丝,使胶孢炭疽菌菌丝膨胀崩解。菌株NQ7GII4的培养滤液在浓度为15%时对病原菌菌丝的抑制率达到70.75%,菌株NQ8GII7产生的挥发性物质对胶孢炭疽菌抑制率高达83.44%。离体生防试验结果表明,用拮抗性内生真菌预先占位接种枸杞嫩果及叶片,能有效抑制枸杞炭疽病菌的侵入和病斑的扩展,菌株NQ8GII410%培养滤液对枸杞炭疽病的防治效果最强,防效达到了92.54%和95.57%,说明枸杞内生真菌具有很好的生防潜力和应用前景。 展开更多
关键词 枸杞内生真菌 胶孢炭疽菌 拮抗作用 生物防治
下载PDF
Carbendazim sensitivity in populations of Colletotrichum gloeosporioides complex infecting strawberry and yams in Hubei Province of China 被引量:11
11
作者 HAN Yong-chao ZENG Xiang-guo +4 位作者 XlANG Fa-yun ZHANG Qing-hua GUO Cong CHEN Feng-ying GU Yu-chen 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第6期1391-1400,共10页
The ascomycete fungus Colletotrichum gloeosporioides is a devastating plant pathogen with a wide host range and worldwide distribution. Carbendazim has been widely used to control anthracnose caused by the C. gloeospo... The ascomycete fungus Colletotrichum gloeosporioides is a devastating plant pathogen with a wide host range and worldwide distribution. Carbendazim has been widely used to control anthracnose caused by the C. gloeosporioides complex in China for more than 30 years and resistance to carbendazim has been reported in China. A total of 125 Colletotrichum isolates of strawberry and yam were collected from different geographical regions in Hubei Province, China. Approximately 52.8% of Colletotrichum spp. isolates showed resistance to carbendazim. The isolates tested in this study belong to four species, and the frequencies of resistant isolates differed across Colletotrichum species. Resistant isolates were found in C. siamense and C. fructicola. In contrast, all isolates of C. gloeosporioides and C. aenigma were sensitive to carbendazim. Highly carbendazim-resistant isolates harbored the E198A mutation in the β-tubulin 2 (TUB2) gene, whereas moderately carbendazim-resistant isolates harbored the F200Y mutation in the TUB2 gene. Carbendazim-sensitive Colletotrichum isolates in this study were not genetically similar enough to form a separate cluster from resistant isolates. The result of this study emphasizes the importance of knowing which Colletotrichum sp. is present, when strategies for disease control are made. 展开更多
关键词 CARBENDAZIM resistance frequency colletotrichum gloeosporioides colletotrichum species point mutations ANTHRACNOSE
下载PDF
Biological Control Potential of <i>Colletotrichum gloeosporioides</i>for Coffee Senna (<i>Cassia occidentalis</i>)
12
作者 Clyde D. Boyette Robert E. Hoagland +1 位作者 Mark A. Weaver Kenneth Stetina 《American Journal of Plant Sciences》 2012年第4期430-436,共7页
A fungal pathogen, Colletotrichum gloeosporioides was isolated from a greenhouse-grown seedling of coffee senna (Cassia occidentalis) and evaluated as a mycoherbicide for that weed. Host range tests revealed that coff... A fungal pathogen, Colletotrichum gloeosporioides was isolated from a greenhouse-grown seedling of coffee senna (Cassia occidentalis) and evaluated as a mycoherbicide for that weed. Host range tests revealed that coffee senna, wild senna (C. marilandica), and sicklepod (C. obtusifolia) were also affected by this pathogen, but 35 other crop and weed species, representing 8 botanical families were not affected. The fungus sporulated prolifically on solid and liquid media with maximum spore germination and growth occurring at 20°C - 30°C. Optimal environmental conditions included at least 12 h of free moisture (dew) at 20°C - 30°C. Spray mixtures containing approximately 1.0 × 105 or more conidia·ml–1 gave maximum control when coffee senna seedlings were sprayed until runoff occurred. Coffee senna seedlings that were in the cotyledon to first-leaf growth stage were most susceptible to this pathogen. Weed control efficacy studies under field conditions demonstrated that control of coffee senna was directly proportional to the inoculum concentration applied. Results of these tests suggest that this fungus has potential as a mycoherbicide to control coffee senna, a serious weed in the southeastern U.S. 展开更多
关键词 BIOHERBICIDE MYCOHERBICIDE COFFEE SENNA Cassia occidentalis colletotrichum gloeosporioides
下载PDF
Effects of Boron on Spore Germination and Integrity of Colletotrictum gloeosporioides( Penz) Saec
13
作者 Ying LU Yixian XIE +1 位作者 Lei ZHANG Songgang LI 《Agricultural Biotechnology》 CAS 2014年第6期38-41,50,共5页
Litchi anthracnose caused by Colletotrictum gloeosporioides (Penz) Saec. is an extremely destructive and widely distributed disease, which results in poor market value. Borate, an essential plant micronutrient that ... Litchi anthracnose caused by Colletotrictum gloeosporioides (Penz) Saec. is an extremely destructive and widely distributed disease, which results in poor market value. Borate, an essential plant micronutrient that helps plant growth and has been used extensively in industry and agriculture as a safe method for control of fungi, was effective in the form of potassium tetraborate for control of C. gloeosporioides (Penz). In this study, boron strongly inhibited spore germina- tion, germ tube elongation, and mycelial spread of C. gloeosporioides (Penz) in the culture medium. Application of boron at 1% caused the appearance of abnor- mal spores (disrupted) in some cases. On the basis of propidium iodide fluorescent staining, the loss of membrane integrity in C. gloeosporioides (Penz) was ob- served after boron treatment. Furthermore, Boron led to the leakage of cellular constituents (soluble proteins and carbohydrates) from hyphae of C. gloeosporioides (Penz). These data suggest that the mechanisms may be directly related with the disruption effect of boron on cell membrane of the fungal pathogen, resulting in the breakdown of cell membrane structure and loss of cytoplasmic materials from the hyphae. 展开更多
关键词 BORON Colletotrictum gloeosporioides penz Saec Cell membrane breakdown Cellular leakage
下载PDF
Optimization and production of antifungal hydrolysis enzymes by streptomyces aureofaciens against Colletotrichum gloeosporioides of mango
14
作者 Wafaa Mohamed Haggag Enas Mostafa Mohamed Ahamed Mohamed El Azzazy 《Agricultural Sciences》 2011年第2期146-157,共12页
We isolated naturally occurring actinomycetes with an ability to produce metabolites having antifungal property against, Colletotrichum gloeosporioides, the causal agent of mango anthracnose. One promising strain was ... We isolated naturally occurring actinomycetes with an ability to produce metabolites having antifungal property against, Colletotrichum gloeosporioides, the causal agent of mango anthracnose. One promising strain was strong antifungal activity, was selected for further studies. Based on the physiological and biochemical characteristics, the bacterial strain was identical to Streptomyces aureofaciens. Culture filtrate collected from the exponential and stationary phases inhibited the growth of fungus tested, indicating that growth suppression was due to extracellular antifungal metabolites present in culture filtrate. Isolate highly produced extracellular chitinase and β-1,3-glucanase during the exponential and late exponential phases, respectively. In order to standardize the metabolite production some cultural conditions like different incubation time in hours, pH, carbon sources and concentrations and nitrogen source were determined. During fermentation, growth, pH and hydrolysis enzymes production were monitored .Treatment with bioactive components exhibited a significantly high protective activity against development of anthracnose disease on mango trees and increased fruit yield. 展开更多
关键词 ANTIFUNGAL colletotrichum gloeosporioides MANGO ANTHRACNOSE and STREPTOMYCES Aureofaciens.
下载PDF
Rapid detection of the E198A mutation of carbendazim-resistant isolates in Colletotrichum gloeosporioides by loop-mediated isothermal amplification
15
作者 Hongbo Yuan Hui Hou +2 位作者 Zengqiang Zhou Hongtao Tu Li Wang 《Horticultural Plant Journal》 SCIE CSCD 2022年第3期289-296,共8页
Apple bitter rot is a serious agricultural disease caused by Colletotrichum gloeosporioides.In recent years,carbendazim-resistant C.gloeosporioides strains bearing an E198A point mutation in theβ-tubulin gene(GAG to ... Apple bitter rot is a serious agricultural disease caused by Colletotrichum gloeosporioides.In recent years,carbendazim-resistant C.gloeosporioides strains bearing an E198A point mutation in theβ-tubulin gene(GAG to GCG)have emerged,threatening global apple production.As such,rapidly detecting the presence of this E198Amutation in C.gloeosporioides isolates is essential in order to monitor the spread of this pathogen and to prevent outbreaks of disease.Herein,we developed a simple loop-mediated isothermal amplification(LAMP)approach to detecting the E198A mutation in C.gloeosporioides isolates from‘Gala’apple samples.This optimized LAMP protocol was sufficient to establish the E198A genotype of a given isolate following a 60min incubation at 63℃ by using four specific primers.The results of this reaction could be interpreted visually based on a fluorescent yellow-green color change upon the addition of the SYBR Green I dye,and were additionally confirmed via gel electrophoresis.Importantly,this LAMP assay was capable of rapidly and reliably detecting apples that were infected with carbendazim-resistant isolates harboring this E198A mutation.In conclusion,this LAMP assay in this study can rapidly,specifically,and sensitively detect cases of apple bitter rot caused by C.gloeosporioides isolates harboring the E198A mutation. 展开更多
关键词 Apple bitter rot colletotrichum gloeosporioides Carbendazim resistance Loop-mediated isothermal amplification E198A mutation
下载PDF
Study of the Action Mode of Wickerhamomyces anomalus against Colletotrichum gloeosporioides
16
作者 Luis Femando Zepeda-Giraud Dario Rafael Olicon-Hemandez +1 位作者 Cinthya Martinez-Lopez Guadalupe Guerra-Sanchez 《Journal of Agricultural Science and Technology(B)》 2016年第5期341-349,共9页
Colletotrichum gloeosporioides is the causal agent of anthracnose disease in fruits and vegetables, representing a global problem. The use of biocontrol agents has proved effective against fungal diseases in a wide va... Colletotrichum gloeosporioides is the causal agent of anthracnose disease in fruits and vegetables, representing a global problem. The use of biocontrol agents has proved effective against fungal diseases in a wide variety of products. In this work, the antifungal activity of Wickerhamomyces anomalus against C. gloeosporioides isolated from contaminated avocados was evaluated. The antagonism and volatile compound inhibition were measured on Petri dishes. In the mixed cultures, the mycelia damage was observed by scanning electron microscope (SEM). Chitinase and glucanase production by the antagonism was quantified by the reducing sugars method, and biofilm formation was evaluated with 1% crystal violet. The yeast W. anomalus could reduce the growth of C. gloeosporioides up to 65% by direct antagonism and 10% by volatile compounds. The antagonist did not allow the conidia germination and mycelia growth in any of the tested formulations. SEM showed mycelial damage caused by W. anomalus. The antagonist showed adhesion to the mycelium by a polysaccharide biofilm. The presence of mycelium stimulated the hydrolytic enzyme production with the maximal activity of 21.4 U/mg for chitinases at 24 h and 10 U/mg for glucanases at 60 h. These results showed that W. anomalus used together different mechanisms to express its antifungal activity against C. gloeosporioides. This study might be the first report for this phytopathogen isolated from avocado fruits, which could represent an opportunity to establish biocontrol of diseases for this agricultural product. 展开更多
关键词 colletotrichum gloeosporioides Wickerhamomyces anomalus BIOCONTROL AVOCADO
下载PDF
Antimicrobial and cytotoxic activities of endophytic fungus Colletotrichum gloeosporioides isolated from endemic tree Cinnamomum malabatrum
17
作者 Packiaraj R Jeyakumar S +4 位作者 Ayyappan N Adhirajan N Premkumar G Rajarathinam K Muthuramkumar S 《Studies in Fungi》 2016年第1期104-113,共10页
In a survey of endophytic fungi associated with endemic plant Cinnamomum malabatrum leaves harbored a bioactive endophytic isolate CMS 3 was identified as Colletotrichum gloeosporioides through morphological and phylo... In a survey of endophytic fungi associated with endemic plant Cinnamomum malabatrum leaves harbored a bioactive endophytic isolate CMS 3 was identified as Colletotrichum gloeosporioides through morphological and phylogenetic analysis based on ITS-rDNA.The ethyl acetate extract of fermentation broth of Colletotrichum gloeosporioides CMS 3 displayed antimicrobial activity against gram positive and gram negative bacteria as well as the fungal pathogen,Candida albicans.The ethyl acetate crude extract showed in vitro cytotoxicity against the HeLa,MCF-7 and MG63 cancer cell lines with the IC50 values of 94.2μg/ml,84.3μg/ml and 162μg/ml respectively.Gas chromatography and Mass Spectrophotometry(GC-MS)analysis of crude extract confirmed that CMS 3 was a prolific producer of secondary metabolites,in which nearly 74%of the metabolites not listed in the NIST database.Major compounds were phenol 3,5-dimethoxy acetate(11.82%),4'-isopropylidene-bis-(2-cyclohexyl)phenol,N-Didehydrohexacarboxyl-2,4,5-trimethylpiperazine and 1,2,4-Triazolium ylide.These metabolites may be responsible for its antimicrobial and cytotoxic activities. 展开更多
关键词 Antimicrobial potential colletotrichum gloeosporioides Cytotoxic activity Endemic tree Endophytic fungus GC-MS
下载PDF
葡萄胶孢炭疽病菌复合种(Colletotrichum gloeosporioides species complexes)对嘧菌酯的抗药性研究
18
作者 杨敬辉 许媛 +3 位作者 肖婷 褚姝频 刘吉祥 姚克兵 《园艺学报》 CAS CSCD 北大核心 2024年第8期1906-1912,共7页
研究了江苏丘陵地区葡萄胶孢炭疽病菌复合种(Colletotrichum gloeosporioides species complexes,CGSC)对嘧菌酯的敏感性及抗性分子机理。利用孢子萌发法和区分剂量法确定3个种(C.aenigma、C.viniferum和C.fructicola)51个单孢分离株对... 研究了江苏丘陵地区葡萄胶孢炭疽病菌复合种(Colletotrichum gloeosporioides species complexes,CGSC)对嘧菌酯的敏感性及抗性分子机理。利用孢子萌发法和区分剂量法确定3个种(C.aenigma、C.viniferum和C.fructicola)51个单孢分离株对嘧菌酯的抑制中浓度(EC_(50))和敏感性表型,采用田间人工接种防治方法评价其抗药性,通过嘧菌酯作用标靶基因(CYTB)序列分析确定抗性的分子机制。27个C.aenigma和16个C.viniferum中对嘧菌酯敏感的菌株分别有20和14个,EC_(50)分别为0.0388(0.0150~0.0770)和0.0614(0.0213~0.0906)mg·L^(-1),而抗性菌株的EC_(50)均大于100 mg·L^(-1);C.fructicola菌株均为抗性菌株,其EC_(50)大于100 mg·L^(-1)。田间接种防治试验证明嘧菌酯2倍推荐剂量(250 mg·L^(-1))对抗性菌株C.fructicola MS206失去防效(防治效果7.11%)。所有抗性菌株的CYTB基因上都只含有G143A点突变;CYTB基因G143位氨基酸两侧内元呈现多样性。江苏丘陵地区葡萄炭疽病种群中的不同种对嘧菌酯的敏感性不同,抗药种群的流行是造成田间防效下降的主因子,所有抗性菌株的抗性分子机制均为CYTB基因G143A点突变,未发现其他点突变类型。 展开更多
关键词 葡萄 炭疽病 胶孢炭疽病菌复合种 嘧菌酯 抗药性 分子机制
原文传递
7种杀菌剂对无花果炭疽病的室内毒力及田间防效
19
作者 张继 唐文忠 +3 位作者 唐爱华 郑元涛 胡耿彪 尧金燕 《安徽农业科学》 CAS 2024年第15期152-156,共5页
[目的]筛选出防治无花果炭疽病的有效药剂。[方法]选用7种杀菌剂进行室内毒力测定和田间药效试验。[结果]35%苯醚甲环唑·吡唑醚菌酯悬浮剂、30%吡唑醚菌酯·戊唑醇悬浮剂、30%吡唑醚菌酯·氟硅唑乳油、40%苯醚甲环唑悬浮剂... [目的]筛选出防治无花果炭疽病的有效药剂。[方法]选用7种杀菌剂进行室内毒力测定和田间药效试验。[结果]35%苯醚甲环唑·吡唑醚菌酯悬浮剂、30%吡唑醚菌酯·戊唑醇悬浮剂、30%吡唑醚菌酯·氟硅唑乳油、40%苯醚甲环唑悬浮剂、25%吡唑醚菌酯悬浮剂、430 g/L戊唑醇悬浮剂、40%氟硅唑乳油对无花果炭疽病的4 d-EC 50分别为0.1940、0.0788、0.0978、0.4450、0.2180、0.1650、0.1460 mg/L;7种药剂对无花果树叶片炭疽病的防治结果:30%吡唑醚菌酯·戊唑醇悬浮剂在2000倍液下防效最佳,为92.76%±2.96%,30%吡唑醚菌酯·氟硅唑乳油在2000倍液下防效次之,为89.54%±2.41%,两者间无显著差异。其他药剂在2000倍液下防效均超过80%,30%吡唑醚菌酯·戊唑醇悬浮剂在3000倍液下防效也超过80%,其他处理的防效较差。7种药剂对无花果果实炭疽病的防治结果:30%吡唑醚菌酯·戊唑醇悬浮剂在2000倍液下防效最好,达86.22%±3.87%,30%吡唑醚菌酯·氟硅唑乳油在2000倍液下防效为83.69%±1.59%,430 g/L戊唑醇悬浮剂在2000倍液下防效为81.68%±2.01%,其他处理的防效均低于80%。[结论]30%吡唑醚菌酯·戊唑醇悬浮剂和30%吡唑醚菌酯·氟硅唑乳油在2000倍液下对无花果炭疽病具有较好的防效,可以推广到广西地区无花果种植区的炭疽病防治。 展开更多
关键词 无花果 炭疽病 杀菌剂 毒力 防效
下载PDF
苹果与胶孢炭疽菌互作研究进展
20
作者 冀志蕊 王美玉 +4 位作者 张树武 杜宜南 丛佳林 徐秉良 周宗山 《果树学报》 CAS CSCD 北大核心 2024年第6期1199-1212,共14页
胶孢炭疽菌(Colletotrichum gloeosporioides)能够引发苹果苦腐病和苹果炭疽叶枯病,危害叶片和果实,影响果品产量和品质,给苹果产业造成严重的经济损失。对苹果与病原物互作分子机制最新研究进展进行综述,包括苹果上炭疽病的病原菌组成... 胶孢炭疽菌(Colletotrichum gloeosporioides)能够引发苹果苦腐病和苹果炭疽叶枯病,危害叶片和果实,影响果品产量和品质,给苹果产业造成严重的经济损失。对苹果与病原物互作分子机制最新研究进展进行综述,包括苹果上炭疽病的病原菌组成和分类、侵染循环及其引发的果树病害种类,病原菌的致病结构和降解酶类、致病相关基因的挖掘与分析、效应蛋白的筛选与功能分析等致病相关分子机制,苹果被侵染后生理生化变化、激素信号、抗病基因挖掘、miRNA参与的免疫调控机制等抗病相关的研究内容,以期为解析病原菌致病机制及与寄主互作机制,进而为挖掘潜力候选基因,以及病害综合防控和抗病分子育种奠定理论基础。 展开更多
关键词 苹果 胶孢炭疽菌 侵染机制 抗病机制
下载PDF
上一页 1 2 30 下一页 到第
使用帮助 返回顶部