Post-stroke depression is associated with reduced expression of brain-derived neurotrophic factor （BDNF）. In this study, we evaluated whether BDNF overexpression affects depression-like behavior in a rat model of po...Post-stroke depression is associated with reduced expression of brain-derived neurotrophic factor （BDNF）. In this study, we evaluated whether BDNF overexpression affects depression-like behavior in a rat model of post-stroke depression. The middle cerebral artery was occluded to produce a model of focal cerebral ischemia. These rats were then subjected to isolation-housing combined with chronic unpredictable mild stress to generate a model of post-stroke depression. A BDNF gene lentiviral vector was injected into the hippocampus. At 7 days after injection, western blot assay and real-time quantitative PCR revealed that BDNF expression in the hippo- campus was increased in depressive rats injected with BDNF lentivirus compared with depressive rats injected with control vector. Furthermore, sucrose solution consumption was higher, and horizontal and vertical movement scores were increased in the open field test in these rats as well. These findings suggest that BDNF overexpression in the hippocampus of post-stroke depressive rats alleviates depression-like behaviors.展开更多
目的探讨格拉斯哥昏迷评分(glasgow coma scale,GCS)、因斯布鲁克昏迷评分(innsbruck comascale,ICS)和急性生理和慢性健康评分Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)对昏迷患者脑功能及预后判断的评价价值。...目的探讨格拉斯哥昏迷评分(glasgow coma scale,GCS)、因斯布鲁克昏迷评分(innsbruck comascale,ICS)和急性生理和慢性健康评分Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)对昏迷患者脑功能及预后判断的评价价值。方法按3种临床评分标准对GCS3~8分、入院3 d的68例昏迷患者进行评分,并对所得评分结果进行分级、对比,分析其与患者预后的相关性。结果各临床分级标准与预后均有较好的相关性,3种临床评分标准比较,其预测预后的准确性差异无统计学意义(P<0.05)。结论GCS、ICS和APACHEⅡ3种临床评分量表均能对昏迷患者的预后提供良好的诊断价值,3种标准间预测价值差异不明显。展开更多
基金supported by a grant from the Experimental Animal Science and Technology Project of Zhejiang Province in China,No.2012C37083
文摘Post-stroke depression is associated with reduced expression of brain-derived neurotrophic factor （BDNF）. In this study, we evaluated whether BDNF overexpression affects depression-like behavior in a rat model of post-stroke depression. The middle cerebral artery was occluded to produce a model of focal cerebral ischemia. These rats were then subjected to isolation-housing combined with chronic unpredictable mild stress to generate a model of post-stroke depression. A BDNF gene lentiviral vector was injected into the hippocampus. At 7 days after injection, western blot assay and real-time quantitative PCR revealed that BDNF expression in the hippo- campus was increased in depressive rats injected with BDNF lentivirus compared with depressive rats injected with control vector. Furthermore, sucrose solution consumption was higher, and horizontal and vertical movement scores were increased in the open field test in these rats as well. These findings suggest that BDNF overexpression in the hippocampus of post-stroke depressive rats alleviates depression-like behaviors.
文摘目的探讨格拉斯哥昏迷评分(glasgow coma scale,GCS)、因斯布鲁克昏迷评分(innsbruck comascale,ICS)和急性生理和慢性健康评分Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)对昏迷患者脑功能及预后判断的评价价值。方法按3种临床评分标准对GCS3~8分、入院3 d的68例昏迷患者进行评分,并对所得评分结果进行分级、对比,分析其与患者预后的相关性。结果各临床分级标准与预后均有较好的相关性,3种临床评分标准比较,其预测预后的准确性差异无统计学意义(P<0.05)。结论GCS、ICS和APACHEⅡ3种临床评分量表均能对昏迷患者的预后提供良好的诊断价值,3种标准间预测价值差异不明显。
