Comamonas testosteroni(C. testosteroni) has been rarely observed as an infectious agent in clinical practice. Few reports described its potential pathogenicity in bloodstream and abdominal infections. Here, we report ...Comamonas testosteroni(C. testosteroni) has been rarely observed as an infectious agent in clinical practice. Few reports described its potential pathogenicity in bloodstream and abdominal infections. Here, we report our experience in the treatment of a C. testosteroni-associated peritonitis in a four-year-old girl receiving chronic peritoneal dialysis(PD). The organism was shown to be highly susceptible to appropriate antibiotic therapy. Infection responded promptly and the patient was managed conservatively without withdrawal from PD.展开更多
BACKGROUND Comamonas kerstersii(C.kerstersii)infections have considered as non-pathogenic to humans,however due to new techniques such as matrix-assisted laser desorption ionization-time of flight mass spectrometry(MA...BACKGROUND Comamonas kerstersii(C.kerstersii)infections have considered as non-pathogenic to humans,however due to new techniques such as matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF-MS),more cases have been identified.CASE SUMMARY This is the first report of a maternal patient with a C.kerstersii bacteremia following caesarean section.Due to the severity of the patient’s condition;high fever and rapidly progressing organ damage,the patient was transferred to the intensive care unit.C.kerstersii was detected by metagenomic next-generation sequencing testing.Based on the drug sensitivity test,appropriate antibiotic treatment was given and the patient recovered fully.CONCLUSION This case report confirms that the detection via MALDI-TOF-MS and metagenomic next-generation sequencing testing provides a reliable basis for the diagnosis of this rare bacterial infection.展开更多
Well-channeled porous polyethersulfone（PES） beads were synthesized and used for the immobilization of Comamonas testosteroni QYY cells for an aerobic reactor to remove quinoline, phenol, and other refractory com- po...Well-channeled porous polyethersulfone（PES） beads were synthesized and used for the immobilization of Comamonas testosteroni QYY cells for an aerobic reactor to remove quinoline, phenol, and other refractory com- pounds in accidentally-released dye wastewater supplemented with domestic wastewater. The pore size in PES beads mainly depended on the dripping time through the Water vapor cylinder. When the cylinder was 2.5 m in length, the pore size in the obtained beads was enlarged to 3 ~m, which provided an ideal surface for cells to pass through and grow inside. The reactor with the immobilized C. testosteroni QYY on PES beads resisted organic loading shock and enhanced total organic carbon（TOC） removal, which had 100% removal efficiencies of both quinoline and phenol when the volume ratio of the accidental wastewater to domestic wastewater was increased from 1：2 to 1：1, as compared with the 100% and 34.7% removal efficiencies by the reactor with immobilized C. testosteroni QYY on polyttrethane（PU） cube or the 82% and 2.4% removal efficiencies by the reactor with only the suspended C. testosteroni QYY cells, respectively. The PES beads had a specific surface area of 1843 cm2/cm3, which had immobilized （0.0244-0.003） g of C, testosteroni QYY cell dry mass/cm3, compared with the specific surface area of 564 cm2/cm3 of the PU cube with （0.0184-0.002） g of cell dry mass/cm3. The kinetic study revealed that the quinoline and phenol degradation followed zero-order reactions for all the three reactors. The PES reactor demonstrated the highest quinoline and phenol removal efficiencies. The immobilized C. testosteroni QYY on the low-cost inert PES beads demonstrated good shock resistance and was able to completely remove the toxic compounds, including phenyl carbamate, 2-nitrotoluene, and dioctyl phthalate. Therefore, the beads were ideal for large-scale accidental wastewater treatment.展开更多
Delftia acidovorans(earlier known as Comamonas acidovorans) is an aerobic,non-fermentative, Cram negative rod,classified in the Pseudomonas rRNA homology Group 111.Reports of isolation of the organism from serious inf...Delftia acidovorans(earlier known as Comamonas acidovorans) is an aerobic,non-fermentative, Cram negative rod,classified in the Pseudomonas rRNA homology Group 111.Reports of isolation of the organism from serious infections like central venoug catheter associated bacleremia. corneal ulcers,otitis media exist.The microbiologists can identify this organism based on an orange indole reaction.This reaction demonstrates the organism's ability to produce anthranilic acid from tryptophan on addition of Kovac's reagent;which gives the media its characteristic "pumpkin orange" colour.Here we report the isolation of this organism from the Endotracheal tube aspirate of a 4 year old child.With the increasing use of invasive devices,it has become important to recognize these non fermentative gram negative bacilli as emerging source of infection even in immunocompetent individuals.展开更多
文摘Comamonas testosteroni(C. testosteroni) has been rarely observed as an infectious agent in clinical practice. Few reports described its potential pathogenicity in bloodstream and abdominal infections. Here, we report our experience in the treatment of a C. testosteroni-associated peritonitis in a four-year-old girl receiving chronic peritoneal dialysis(PD). The organism was shown to be highly susceptible to appropriate antibiotic therapy. Infection responded promptly and the patient was managed conservatively without withdrawal from PD.
文摘BACKGROUND Comamonas kerstersii(C.kerstersii)infections have considered as non-pathogenic to humans,however due to new techniques such as matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF-MS),more cases have been identified.CASE SUMMARY This is the first report of a maternal patient with a C.kerstersii bacteremia following caesarean section.Due to the severity of the patient’s condition;high fever and rapidly progressing organ damage,the patient was transferred to the intensive care unit.C.kerstersii was detected by metagenomic next-generation sequencing testing.Based on the drug sensitivity test,appropriate antibiotic treatment was given and the patient recovered fully.CONCLUSION This case report confirms that the detection via MALDI-TOF-MS and metagenomic next-generation sequencing testing provides a reliable basis for the diagnosis of this rare bacterial infection.
基金Supported by the Major Science and Technology Program for Water Pollution Control and Treatment of China（No. 2014ZX07201-011-004）, the National Natural Science Foundation of China（Nos.51578118, 51378098, 51238001, 51408110, 51508078, 51478095, 51478096）, the Natural Science Foundation of Jilin Province, China（Nos.20150101072JC, 20140520151JH, 20160520023JH）, the Jilm Province Education Department Science and Technology Research Project, China（No.2014B043） and the Program for New Century Excellent Talents in University of the Ministry of Education of China（No.NCET-13-0723）.
文摘Well-channeled porous polyethersulfone（PES） beads were synthesized and used for the immobilization of Comamonas testosteroni QYY cells for an aerobic reactor to remove quinoline, phenol, and other refractory com- pounds in accidentally-released dye wastewater supplemented with domestic wastewater. The pore size in PES beads mainly depended on the dripping time through the Water vapor cylinder. When the cylinder was 2.5 m in length, the pore size in the obtained beads was enlarged to 3 ~m, which provided an ideal surface for cells to pass through and grow inside. The reactor with the immobilized C. testosteroni QYY on PES beads resisted organic loading shock and enhanced total organic carbon（TOC） removal, which had 100% removal efficiencies of both quinoline and phenol when the volume ratio of the accidental wastewater to domestic wastewater was increased from 1：2 to 1：1, as compared with the 100% and 34.7% removal efficiencies by the reactor with immobilized C. testosteroni QYY on polyttrethane（PU） cube or the 82% and 2.4% removal efficiencies by the reactor with only the suspended C. testosteroni QYY cells, respectively. The PES beads had a specific surface area of 1843 cm2/cm3, which had immobilized （0.0244-0.003） g of C, testosteroni QYY cell dry mass/cm3, compared with the specific surface area of 564 cm2/cm3 of the PU cube with （0.0184-0.002） g of cell dry mass/cm3. The kinetic study revealed that the quinoline and phenol degradation followed zero-order reactions for all the three reactors. The PES reactor demonstrated the highest quinoline and phenol removal efficiencies. The immobilized C. testosteroni QYY on the low-cost inert PES beads demonstrated good shock resistance and was able to completely remove the toxic compounds, including phenyl carbamate, 2-nitrotoluene, and dioctyl phthalate. Therefore, the beads were ideal for large-scale accidental wastewater treatment.
文摘Delftia acidovorans(earlier known as Comamonas acidovorans) is an aerobic,non-fermentative, Cram negative rod,classified in the Pseudomonas rRNA homology Group 111.Reports of isolation of the organism from serious infections like central venoug catheter associated bacleremia. corneal ulcers,otitis media exist.The microbiologists can identify this organism based on an orange indole reaction.This reaction demonstrates the organism's ability to produce anthranilic acid from tryptophan on addition of Kovac's reagent;which gives the media its characteristic "pumpkin orange" colour.Here we report the isolation of this organism from the Endotracheal tube aspirate of a 4 year old child.With the increasing use of invasive devices,it has become important to recognize these non fermentative gram negative bacilli as emerging source of infection even in immunocompetent individuals.
