Comblike poly(methyl methacrylate) was synthesized by atom transfer radical polymerization of methyl methacrylate with poly(ethyl 2-bromoacrylate) as a macroinitiator, which was prepared by conventional free radical p...Comblike poly(methyl methacrylate) was synthesized by atom transfer radical polymerization of methyl methacrylate with poly(ethyl 2-bromoacrylate) as a macroinitiator, which was prepared by conventional free radical polymerization of ethyl 2-bromoacrylate. The obtained comblike polymers were characterized by GPC and 1H NMR.展开更多
A new kind of comblike copolymer film composed of acrylic acid-polyethylene glycol monomethyl ether acrylate copolymer（AA-PEGMA copolymer） was successfully synthesized to immobilize hemoglobin（Hb）. FTIR, UV-Vis an...A new kind of comblike copolymer film composed of acrylic acid-polyethylene glycol monomethyl ether acrylate copolymer（AA-PEGMA copolymer） was successfully synthesized to immobilize hemoglobin（Hb）. FTIR, UV-Vis and CD spectra suggest that Hb kept its original structure in the AA-PEGMA copolymer film without denaturation. A pair of well-defined, quasi-reversible cyclic voltammetric peaks at around –270 mV vs. saturated calomel electrode（SCE） for the Hb Fe（III）/Fe（II） redox couple was observed on the film-modified electrode in phos phate buffer solution（PBS, pH=7.0）. The formal potential of Hb/AA-PEGMA copolymer film-modified electrode is linearly dependent on solution pH with a slope of –46.3 mV/pH, illustrating that one-proton transfer was accompa nied with each electron transfer. Furthermore, the modified electrode displayed electrocatalytic response to the reduc tion of H2O2 with a linear range of 3.5―126 μmol/L and a detection limit of 1.17 μmol/L. In conclusion, the AA-PEGMA copolymer film was proved to be an excellent matrix for the immobilization and electrochemistry of proteins.展开更多
The effect of temperature-responsive solution behavior of PNIPAM-b-PPEOMA-b-PNIPAM on its inclusion complexation with α-cyclodextrin was studied. The triblock polymer was prepared by reversible addition-fragmentation...The effect of temperature-responsive solution behavior of PNIPAM-b-PPEOMA-b-PNIPAM on its inclusion complexation with α-cyclodextrin was studied. The triblock polymer was prepared by reversible addition-fragmentation chain transfer （RAFT） polymerization and formed inclusion complexes （ICs） after selective threading of the PEO segment of the triblock polymer through the cavities of α-CD units. For comparison, PPEOMA homopolymer was prepared and the inclusion complexation with α-CD was also studied. The ICs were prepared with a-CD when the polymer was in different conformations by changing the temperature, and the formed ICs were characterized by XRD, ^1H-NMR, TGA and DSC. The solutions of the ICs show temperature-responsive clear/turbid transition or fluidic emulsion/gel transition depending on the concentration of the α-CD added, and the stoichiometry determined by ^1H-NMR and TGA indicates that the stoichiometry of EO to α-CD of the resulted ICs increases with increasing of temperature.展开更多
文摘Comblike poly(methyl methacrylate) was synthesized by atom transfer radical polymerization of methyl methacrylate with poly(ethyl 2-bromoacrylate) as a macroinitiator, which was prepared by conventional free radical polymerization of ethyl 2-bromoacrylate. The obtained comblike polymers were characterized by GPC and 1H NMR.
基金Supported by the National Natural Science Foundation of China(No.21076065)
文摘A new kind of comblike copolymer film composed of acrylic acid-polyethylene glycol monomethyl ether acrylate copolymer（AA-PEGMA copolymer） was successfully synthesized to immobilize hemoglobin（Hb）. FTIR, UV-Vis and CD spectra suggest that Hb kept its original structure in the AA-PEGMA copolymer film without denaturation. A pair of well-defined, quasi-reversible cyclic voltammetric peaks at around –270 mV vs. saturated calomel electrode（SCE） for the Hb Fe（III）/Fe（II） redox couple was observed on the film-modified electrode in phos phate buffer solution（PBS, pH=7.0）. The formal potential of Hb/AA-PEGMA copolymer film-modified electrode is linearly dependent on solution pH with a slope of –46.3 mV/pH, illustrating that one-proton transfer was accompa nied with each electron transfer. Furthermore, the modified electrode displayed electrocatalytic response to the reduc tion of H2O2 with a linear range of 3.5―126 μmol/L and a detection limit of 1.17 μmol/L. In conclusion, the AA-PEGMA copolymer film was proved to be an excellent matrix for the immobilization and electrochemistry of proteins.
基金financially supported by the Hunan Normal University for providing start-up
文摘The effect of temperature-responsive solution behavior of PNIPAM-b-PPEOMA-b-PNIPAM on its inclusion complexation with α-cyclodextrin was studied. The triblock polymer was prepared by reversible addition-fragmentation chain transfer （RAFT） polymerization and formed inclusion complexes （ICs） after selective threading of the PEO segment of the triblock polymer through the cavities of α-CD units. For comparison, PPEOMA homopolymer was prepared and the inclusion complexation with α-CD was also studied. The ICs were prepared with a-CD when the polymer was in different conformations by changing the temperature, and the formed ICs were characterized by XRD, ^1H-NMR, TGA and DSC. The solutions of the ICs show temperature-responsive clear/turbid transition or fluidic emulsion/gel transition depending on the concentration of the α-CD added, and the stoichiometry determined by ^1H-NMR and TGA indicates that the stoichiometry of EO to α-CD of the resulted ICs increases with increasing of temperature.
