Mature-green tomato fruit （Solanum lycopersicum cv. Zhenfen 202） were exposed to different UV-C irradiation at 2, 4, 8, and 16 kJ m-2 and then stored under the dark at 14°C and 95% relative humidity （RH） for ...Mature-green tomato fruit （Solanum lycopersicum cv. Zhenfen 202） were exposed to different UV-C irradiation at 2, 4, 8, and 16 kJ m-2 and then stored under the dark at 14°C and 95% relative humidity （RH） for 35 d. Of these four doses, UV-C irradiation at 4 and 8 kJ m-2 significantly increased total phenolic contents in present tomato fruit by 21.2 and 20.2%, respectively. Furthermore, UV-C irradiation at 4 or 8 kJ m-2 promoted the accumulation of total flavonoids and increased the antioxidant activity. 2 or 16 kJ m-2 UV-C irradiation also enhanced antioxidant activity, but to a lesser extent. Seven phenolic compounds, viz., gallic acid, （＋）-catechin, chlorogenic acid, cafferic acid, syringic acid, p-coumaric acid, and quercetin in tomato fruit were identified and quantified by HPLC. Gallic acid was the major phenolic compound in tomato fruit and significantly correlated with antioxidant activity. 4 or 8 kJ m-2 UV-C irradiation significantly increased the contents of gallic acid, chlorogenic acid, syringic acid, p-coumaric acid, and quercetin. The optimum dose of UV-C irradiation in terms of increased phenolic compound content and enhanced Antioxidant activity was determined to be 4 or 8 kJ m-2.展开更多
Over-expression of glutathione S-transferase(GST)can promote Cisplatin resistance in hepatocellular carcinoma(HCC)treatment.Hence,inhibiting GST is an attractive strategy to improve Cisplatin sensitivity in HCC therap...Over-expression of glutathione S-transferase(GST)can promote Cisplatin resistance in hepatocellular carcinoma(HCC)treatment.Hence,inhibiting GST is an attractive strategy to improve Cisplatin sensitivity in HCC therapy.Although several synthesized GST inhibitors have been developed,the side effects and narrow spectrum for anticancer seriously limit their clinical application.Considering the abundance of natural compounds with anticancer activity,this study developed a rapid fluorescence technique to screen“green”natural GST inhibitors with high specificity.The fluorescence assay demonstrated that schisanlactone B(hereafter abbreviated as C1)isolated from Xue tong significantly down-regulated GST levels in Cisplatin-resistant HCC cells in vitro and in vivo.Importantly,C1 can selectively kill HCC cells from normal liver cells,effectively improving the therapeutic effect of Cisplatin on HCC mice by downregulating GST expression.Considering the high GST levels in HCC patients,this compound demonstrated the high potential for sensitizing HCC therapy in clinical practice by down-regulating GST levels.展开更多
The effect of acid component including various conventional acids and tungstic compounds on glucose hydrogenation over a series of binary catalyst system containing Ru/C catalyst was investigated. The results showed t...The effect of acid component including various conventional acids and tungstic compounds on glucose hydrogenation over a series of binary catalyst system containing Ru/C catalyst was investigated. The results showed that HC1, H2SO4, H3BO3, H3PO4, and HNO3 had negligible effect, while all the tungstic compounds imposed inhibiting effects on the hydrogenation of glucose over Ru/C catalyst, and the suppressing effect followed the order of H2WO4〉HPW〉WO3〉AMT〉HSiW. This order is the same as the order of ethylene glycol （EG） yields in the one-pot conversion of glucose to EG, suggesting the important role of competition between glucose hydrogenation and retro-aldol condensation in controlling the selectivity of EG.展开更多
Cubic C3N4 compound in the C-N thin films on Si and NaCl substrates was prepared by ion beam sputtering of a pure graphite target with discharge gas of pure N2. X-ray photoelectron spectroscopy indicated that nitrogen...Cubic C3N4 compound in the C-N thin films on Si and NaCl substrates was prepared by ion beam sputtering of a pure graphite target with discharge gas of pure N2. X-ray photoelectron spectroscopy indicated that nitrogen atoms combined with sp2- and sp3- coordinated C atoms in the film, respectively. X-ray diffraction, selected area electron diffraction and high-resolution electron microscopy were used to identify the cubic C3N4 phase. The results reconfirm the ab initio calculations on metastable structure in C-N compounds展开更多
The title compound 1 was synthesized from 1.2:5,6-dianhydro-3,4-dianhydro-3,4-isopropylideneD-mannitol 2 through four steps in a total yield of 53%. The absolute configuration of this compound was confirmed by X-ray d...The title compound 1 was synthesized from 1.2:5,6-dianhydro-3,4-dianhydro-3,4-isopropylideneD-mannitol 2 through four steps in a total yield of 53%. The absolute configuration of this compound was confirmed by X-ray diffraction.展开更多
The aggregating properties of Cz-C-n (n = 3, 6, 10) have been investigated by means of fluorescence method in DMSO-H2O binary solvent. The measured CAC and C F values indicate that the aggregating tendency of the amp...The aggregating properties of Cz-C-n (n = 3, 6, 10) have been investigated by means of fluorescence method in DMSO-H2O binary solvent. The measured CAC and C F values indicate that the aggregating tendency of the amphiphilic compounds Cz-C-n containing crown ether increases with the length of alkyl chains, similar to that of carbazole compounds with long alkyl chains.展开更多
Ni–Ga alloy(Ni/Ga atomic ratio of 8),Ni3Ga and Ni5Ga3 intermetallic compounds(IMCs)catalysts were prepared from Ni–Mg-Al-Ga layered double hydroxides(LDHs)for the deoxygenation of methyl esters to hydrocarbons.In th...Ni–Ga alloy(Ni/Ga atomic ratio of 8),Ni3Ga and Ni5Ga3 intermetallic compounds(IMCs)catalysts were prepared from Ni–Mg-Al-Ga layered double hydroxides(LDHs)for the deoxygenation of methyl esters to hydrocarbons.In the alloy and IMCs,the presence of Ga reduced the surface Ni atom density,and the charge transfer from Ga to Ni increased the electron density of Ni.In the deoxygenation of methyl laurate,the Ni catalyst gave a complete hydrogenolysis of methyl laurate to CH4at 330°C and 3.0 MPa,while the presence of Ga promoted the HDO pathway and suppressed C–C bond hydrogenolysis and methanation.The Ni5Ga3 catalyst exhibited the best desired performance.Even at 400°C,it gave the yield of C11 and C12 hydrocarbons of ~99%,and the selectivity to CH4(SCH4) was only 2.4%.In the deoxygenation of methyl octanoate and methyl palmitate,the Ni5Ga3 catalyst also gave the yield of hydrocarbons above95%.Reactivity evaluation and methyl propionate-TPD and TPSR results indicate that the C–OCH3 bond instead of the O–CH3 one was cleaved on both Ni and bimetallic Ni–Ga catalysts.It is highlighted that methanol,derived from the C–OCH3 bond hydrogenolysis,mainly decomposed to CO and H2 on IMCs,while it was converted to methane on metallic Ni and alloy.It is of great significance that H2 could be yielded from the methyl ester itself.In short,the utilization of Ni–Ga IMCs can effectively reduce carbon loss and H2 consumption,all of which are ascribed to the geometric and electronic effects of Ga.展开更多
A systematic study of the synthesis of C.I.Acid Blue 9 leuco compound in water is reported.The kinetic analysis of experimental data for the condensation reaction between 2-formylbenzenesulfonic acid sodium and N-ethy...A systematic study of the synthesis of C.I.Acid Blue 9 leuco compound in water is reported.The kinetic analysis of experimental data for the condensation reaction between 2-formylbenzenesulfonic acid sodium and N-ethyl-N-(3'-sulfonic acid benzyl) aniline obtained at four different temperatures ranging between 85 and 100°C is discussed.It is shown that the reaction followed second-order rate kinetics.The overall rate constant(k) increased with the increase of temperature.On the basis of the value of k,activation energy(E_a) of the reaction was evaluated.Importantly,it is found that reactant concentration has great effect on the formation of C.I.Acid Blue 9 leuco compound,implying that it is not enough to improve the conversion of N-ethyl-N-(3'-sulfonic acid benzyl) aniline by only prolonging reaction time in the late period of the reaction.展开更多
为了研究短波紫外线处理对桃果实采后酚类物质代谢和贮藏品质的影响,本试验以玉露桃果实为材料,研究了3.0 k J·m-2UV-C处理对桃果实在25℃8 d贮藏期间酚类物质代谢关键酶活性和果实抗氧化能力的影响。结果表明,3.0 k J·m-2UV-...为了研究短波紫外线处理对桃果实采后酚类物质代谢和贮藏品质的影响,本试验以玉露桃果实为材料,研究了3.0 k J·m-2UV-C处理对桃果实在25℃8 d贮藏期间酚类物质代谢关键酶活性和果实抗氧化能力的影响。结果表明,3.0 k J·m-2UV-C处理能显著抑制桃果实采后可滴定酸含量的下降,抑制果实维生素C含量的损失,增强果实采后着色能力,从而保持果实的感官和食用品质。进一步的研究发现,3.0 k J·m-2UV-C处理诱导了果实PAL活性的升高,增强了果实贮藏初期苯丙烷代谢强度,增加了酚类物质的积累。另外,UV-C处理抑制了贮藏后期PPO和POD活性,减轻了由PPO和POD参与的酚类物质的酶促氧化,减少了桃果实中抗氧化组分含量的下降,维持了果实贮藏期间较高的DPPH自由基清除能力。可见适宜剂量的UV-C处理(3.0 k J·m-2)在桃果实采后贮运保鲜和抗氧化能力调控中具有潜在的应用价值。展开更多
基金Supported by the National Natural Science Foundation of China (30972036)
文摘Mature-green tomato fruit （Solanum lycopersicum cv. Zhenfen 202） were exposed to different UV-C irradiation at 2, 4, 8, and 16 kJ m-2 and then stored under the dark at 14°C and 95% relative humidity （RH） for 35 d. Of these four doses, UV-C irradiation at 4 and 8 kJ m-2 significantly increased total phenolic contents in present tomato fruit by 21.2 and 20.2%, respectively. Furthermore, UV-C irradiation at 4 or 8 kJ m-2 promoted the accumulation of total flavonoids and increased the antioxidant activity. 2 or 16 kJ m-2 UV-C irradiation also enhanced antioxidant activity, but to a lesser extent. Seven phenolic compounds, viz., gallic acid, （＋）-catechin, chlorogenic acid, cafferic acid, syringic acid, p-coumaric acid, and quercetin in tomato fruit were identified and quantified by HPLC. Gallic acid was the major phenolic compound in tomato fruit and significantly correlated with antioxidant activity. 4 or 8 kJ m-2 UV-C irradiation significantly increased the contents of gallic acid, chlorogenic acid, syringic acid, p-coumaric acid, and quercetin. The optimum dose of UV-C irradiation in terms of increased phenolic compound content and enhanced Antioxidant activity was determined to be 4 or 8 kJ m-2.
基金supported by the National Natural Science Foundation of China(Grant Nos.:82003931,82204766 and 81374062)the Outstanding Youth Foundation of Hunan Provincial Education Department of China(Grant No.:20B445)+3 种基金the Hunan Youth Science and Technology Innovation Talents Project,China(Grant No.:2021RC3100)the Chinese Postdoctoral Science foundation(Grant No.:2021M690974)Changjiang Scholars Program in Ministry Education,People's Republic of China(Program No.:T2019133)the Scientific Research Project of Hunan Provincial Education Department(Project No.:21B0394).
文摘Over-expression of glutathione S-transferase(GST)can promote Cisplatin resistance in hepatocellular carcinoma(HCC)treatment.Hence,inhibiting GST is an attractive strategy to improve Cisplatin sensitivity in HCC therapy.Although several synthesized GST inhibitors have been developed,the side effects and narrow spectrum for anticancer seriously limit their clinical application.Considering the abundance of natural compounds with anticancer activity,this study developed a rapid fluorescence technique to screen“green”natural GST inhibitors with high specificity.The fluorescence assay demonstrated that schisanlactone B(hereafter abbreviated as C1)isolated from Xue tong significantly down-regulated GST levels in Cisplatin-resistant HCC cells in vitro and in vivo.Importantly,C1 can selectively kill HCC cells from normal liver cells,effectively improving the therapeutic effect of Cisplatin on HCC mice by downregulating GST expression.Considering the high GST levels in HCC patients,this compound demonstrated the high potential for sensitizing HCC therapy in clinical practice by down-regulating GST levels.
基金supported by the National Natural Science Foundation of China(Grants 21176235 and 21206159)
文摘The effect of acid component including various conventional acids and tungstic compounds on glucose hydrogenation over a series of binary catalyst system containing Ru/C catalyst was investigated. The results showed that HC1, H2SO4, H3BO3, H3PO4, and HNO3 had negligible effect, while all the tungstic compounds imposed inhibiting effects on the hydrogenation of glucose over Ru/C catalyst, and the suppressing effect followed the order of H2WO4〉HPW〉WO3〉AMT〉HSiW. This order is the same as the order of ethylene glycol （EG） yields in the one-pot conversion of glucose to EG, suggesting the important role of competition between glucose hydrogenation and retro-aldol condensation in controlling the selectivity of EG.
文摘Cubic C3N4 compound in the C-N thin films on Si and NaCl substrates was prepared by ion beam sputtering of a pure graphite target with discharge gas of pure N2. X-ray photoelectron spectroscopy indicated that nitrogen atoms combined with sp2- and sp3- coordinated C atoms in the film, respectively. X-ray diffraction, selected area electron diffraction and high-resolution electron microscopy were used to identify the cubic C3N4 phase. The results reconfirm the ab initio calculations on metastable structure in C-N compounds
文摘The title compound 1 was synthesized from 1.2:5,6-dianhydro-3,4-dianhydro-3,4-isopropylideneD-mannitol 2 through four steps in a total yield of 53%. The absolute configuration of this compound was confirmed by X-ray diffraction.
基金We thank the National Natural Science Foundation of China. The foundation of the Ministry of Science and Technology of China (grant No. G2000078104 and No. G2000077502) for financial supports.
文摘The aggregating properties of Cz-C-n (n = 3, 6, 10) have been investigated by means of fluorescence method in DMSO-H2O binary solvent. The measured CAC and C F values indicate that the aggregating tendency of the amphiphilic compounds Cz-C-n containing crown ether increases with the length of alkyl chains, similar to that of carbazole compounds with long alkyl chains.
基金support from the National Natural Science Foundation of China(Nos.21576193 and 21176177)。
文摘Ni–Ga alloy(Ni/Ga atomic ratio of 8),Ni3Ga and Ni5Ga3 intermetallic compounds(IMCs)catalysts were prepared from Ni–Mg-Al-Ga layered double hydroxides(LDHs)for the deoxygenation of methyl esters to hydrocarbons.In the alloy and IMCs,the presence of Ga reduced the surface Ni atom density,and the charge transfer from Ga to Ni increased the electron density of Ni.In the deoxygenation of methyl laurate,the Ni catalyst gave a complete hydrogenolysis of methyl laurate to CH4at 330°C and 3.0 MPa,while the presence of Ga promoted the HDO pathway and suppressed C–C bond hydrogenolysis and methanation.The Ni5Ga3 catalyst exhibited the best desired performance.Even at 400°C,it gave the yield of C11 and C12 hydrocarbons of ~99%,and the selectivity to CH4(SCH4) was only 2.4%.In the deoxygenation of methyl octanoate and methyl palmitate,the Ni5Ga3 catalyst also gave the yield of hydrocarbons above95%.Reactivity evaluation and methyl propionate-TPD and TPSR results indicate that the C–OCH3 bond instead of the O–CH3 one was cleaved on both Ni and bimetallic Ni–Ga catalysts.It is highlighted that methanol,derived from the C–OCH3 bond hydrogenolysis,mainly decomposed to CO and H2 on IMCs,while it was converted to methane on metallic Ni and alloy.It is of great significance that H2 could be yielded from the methyl ester itself.In short,the utilization of Ni–Ga IMCs can effectively reduce carbon loss and H2 consumption,all of which are ascribed to the geometric and electronic effects of Ga.
基金Supported by the National Natural Science Foundation of China(U1608223,21576044,21421005,21536002)the Dalian University of Technology Innovation Team(DUT2016TB12)
文摘A systematic study of the synthesis of C.I.Acid Blue 9 leuco compound in water is reported.The kinetic analysis of experimental data for the condensation reaction between 2-formylbenzenesulfonic acid sodium and N-ethyl-N-(3'-sulfonic acid benzyl) aniline obtained at four different temperatures ranging between 85 and 100°C is discussed.It is shown that the reaction followed second-order rate kinetics.The overall rate constant(k) increased with the increase of temperature.On the basis of the value of k,activation energy(E_a) of the reaction was evaluated.Importantly,it is found that reactant concentration has great effect on the formation of C.I.Acid Blue 9 leuco compound,implying that it is not enough to improve the conversion of N-ethyl-N-(3'-sulfonic acid benzyl) aniline by only prolonging reaction time in the late period of the reaction.
文摘为了研究短波紫外线处理对桃果实采后酚类物质代谢和贮藏品质的影响,本试验以玉露桃果实为材料,研究了3.0 k J·m-2UV-C处理对桃果实在25℃8 d贮藏期间酚类物质代谢关键酶活性和果实抗氧化能力的影响。结果表明,3.0 k J·m-2UV-C处理能显著抑制桃果实采后可滴定酸含量的下降,抑制果实维生素C含量的损失,增强果实采后着色能力,从而保持果实的感官和食用品质。进一步的研究发现,3.0 k J·m-2UV-C处理诱导了果实PAL活性的升高,增强了果实贮藏初期苯丙烷代谢强度,增加了酚类物质的积累。另外,UV-C处理抑制了贮藏后期PPO和POD活性,减轻了由PPO和POD参与的酚类物质的酶促氧化,减少了桃果实中抗氧化组分含量的下降,维持了果实贮藏期间较高的DPPH自由基清除能力。可见适宜剂量的UV-C处理(3.0 k J·m-2)在桃果实采后贮运保鲜和抗氧化能力调控中具有潜在的应用价值。
