Integrated data mining and network pharmacology to discover a novel traditional Chinese medicine prescription against diabetic retinopathy and reveal its mechanism

Background:Diabetic retinopathy(DR)is currently the leading cause of blindness in elderly individuals with diabetes.Traditional Chinese medicine(TCM)prescriptions have shown remarkable effectiveness for treating DR.This study aimed to screen a novel TCM prescription against DR from patents and elucidate its medication rule and molecular mechanism using data mining,network pharmacology,molecular docking and molecular dynamics(MD)simulation.Method:TCM prescriptions for treating DR was collected from patents and a novel TCM prescription was identified using data mining.Subsequently,the mechanism of the novel TCM prescription against DR was explored by constructing a network of core TCMs-core active ingredients-core targets-core pathways.Finally,molecular docking and MD simulation were employed to validate the findings from network pharmacology.Result:The TCMs of the collected prescriptions primarily possessed bitter and cold properties with heat-clearing and supplementing effects,attributed to the liver,lung and kidney channels.Notably,a novel TCM prescription for treating DR was identified,composed of Lycii Fructus,Chrysanthemi Flos,Astragali Radix and Angelicae Sinensis Radix.Twenty core active ingredients and ten core targets of the novel TCM prescription for treating DR were screened.Moreover,the novel TCM prescription played a crucial role for treating DR by inhibiting inflammatory response,oxidative stress,retinal pigment epithelium cell apoptosis and retinal neovascularization through various pathways,such as the AGE-RAGE signaling pathway in diabetic complications and the MAPK signaling pathway.Finally,molecular docking and MD simulation demonstrated that almost all core active ingredients exhibited satisfactory binding energies to core targets.Conclusions:This study identified a novel TCM prescription and unveiled its multi-component,multi-target and multi-pathway characteristics for treating DR.These findings provide a scientific basis and novel insights into the development of drugs for DR prevention and treatment.

Application of Mathematical Models in the Research of Chinese Herbal Compound Prescriptions

With the interdisciplinary development of multiple disciplines,mathematical models play a role as a bridge in the research process of Chinese herbal compounds,which can make full use of the interrelationships of knowledge,making the research and use of Chinese herbal compounds from complex and cumbersome to simple.This paper reviewed the research and application of mathematical models in the production and preparation,pharmacodynamics,pharmacokinetics and other aspects of Chinese herbal compound prescriptions,providing a theoretical basis for the development of the modern Chinese medicine industry.

Research Progress in the Application of Network Pharmacology in Traditional Chinese Medicine and Compound Prescriptions

Network pharmacology is a new discipline based on the theory of systems biology,mainly for network analysis of biological systems.It selects specific signal nodes for multi-target drug molecular design.This article summarizes the application of network pharmacology in traditional Chinese medicine and compound prescriptions in recent years from the research progress of network pharmacology,the current research status of single herbs,and the research and application of compound prescriptions.

Research on the Progress of Treating Gastroesophageal Reflux Disease with Modified Classic Traditional Chinese Medicine Prescription

Gastroesophageal reflux disease (GERD) is a high-incidence digestive system disease. Western medicine mainly uses drugs such as proton pump inhibitors to inhibit gastric acid secretion, but some patients are accompanied by symptoms such as non-acid reflux and gas reflux, which cannot effectively treat the disease. It is necessary to actively explore other treatment schemes. Traditional Chinese medicine (TCM) has a long history of research on gastroesophageal reflux disease, which emphasizes the treatment based on syndrome differentiation as a whole. Through the treatment of various and multi-component TCM prescriptions, the patient’s body condition can be adjusted, and the treatment effect on gastroesophageal reflux disease is reliable, which has obvious therapeutic advantages. To further clarify the treatment of gastroesophageal reflux disease, this study reviewed and analyzed the research progress of the treatment of liver disease with modified prescriptions, and the report is as follows.

China Association of Chinese Medicine Paper notice for seminar on clinical application of TCM classic prescriptions and herbs in the treatment of emotional diseases

Hosted by ChinaAssociation of Chinese Medicine,and organized by World Journal of Integrated traditional and western Medicine,seminar on clinical application of TCM classic prescriptions and herbs in the treatment of emotional diseaseis planned to be held in Yichang,Hubei province from June 22nd to 24th.

Experimental Study on Effect of Compound Biejia Ruangan Prescription (复方鳖甲软肝方) on High Resolution Computerized Tomographic Images in Bleomycin Induced Pulmonary Fibrosis Rats

Objective: To study the therapeutic effect of Com pound Biejia Ruangan prescription (CBRP) on rat model with pulmonary fibrosis in duced by bleomycin. Methods: Fifty four male Sprague Dawley rats were rando mly divided into 6 groups (9 rats in each group). From the first day to the 28th day of the experiment, except to those in the sham model control group that were treated with normal saline, the same amount of bleomycin injection as the n ormal saline given to the control group was given through endotracheal instillat ion to all the rats in all the other groups. From the 29th day of the modeling, CBRP solution of different dosages was respectively injected into the rats in th e high, moderate and low CBRP dose group, while equal volume of normal saline w as given to those in the sham model control group and the model control group , and an equal volume of prednisone solution was given to rats in the prednisone group. On the 80th day, the high resolution computerized tomographic (HRCT) images were observed on an equal footing, and HRCT pathology was correlativel y studied. Results: Different HRCT pathological changes were shown in th e rats with pulmonary fibrosis, such as lung consolidation, thickening of interl obular septum and interlobular mesenchyma as well as lobular deformation, nodule shadow, abnormal brochiovascular tract, thickened pleura with irregular junctio n and polished glass like dense shadows. Honeycomb lung was observed in some cases. Pathological sections showed fibrotic proliferation of lung tissues and noticeable pulmonary interstitial fibrosis. CBRP could improve HRCT images of rats with pulmonary fibrosis, and lower fibrotic p roliferation of the lung tissue.Conclusion: CBRP plays its therapeutic role possibly through its effect on the structure of the lung in rats with pulmonary fibrosis.

Potential Preventive and Therapeutic Effects of Traditional Chinese Medicine Compound Prescription and Traditional Chinese Medicine Products on COVID-19

This paper mainly analyzes the traditional Chinese medicine compound prescription and traditional Chinese medicine products with remarkable epidemic prevention effect since the outbreak of COVID-19,and discusses their potential prevention and treatment effects,so as to provide a basis for the further development and application of traditional Chinese medicine compound prescription and traditional Chinese medicine products against COVID-19.

Analysis on the Pharmacists Intervention Results of the Problems from 2000 Prescriptions of Chinese Herbal Pieces

OBJECTIVE: To interfere with the improper prescriptions of Chinese herbal pieces issued by the outpatient doctors so as to reduce the doctor's prescription error rate and ensure the safety of medication for patients. METHODS:Based on Prescription Administrative Policy, Management Standard of Chinese Herbal Pieces in Hospital, and Beijing Chinese Herbal Pieces Dispensing Regulation, 2000 prescriptions were collected and analyzed according to the different intervention, and solutions were put forward for a variety of reasons. RESULTS: In 2000 prescriptions, there were 932 prescriptions containing toxic drug overdose without double signature, 577 prescriptions of dose error, 282 prescriptions of ingredients error, 85 prescriptions with wrong route of administration, 73 prescriptions of herb incompatibility without double signature, and 51 prescriptions with repeat herbs, accounting for 46.5%, 28.85%, 14.1%, 4.25%, 3.65% and2.55% of the total number of prescriptions respectively. After the intervention of pharmacists, 2000 prescriptions were all successfully intervened to protect the safety of medication. CONCLUSIONS: TCM pharmacist should double check prescriptions with errors issued by doctors, so as to reduce the prescription error rate, and ensure the safety of medication and rehabilitation of patients, and then improve values of pharmacists.

Analysis on the Medication Law of Drug Pair Acorus Tatarinowii-Polygala Tenuifolia Based on the Dictionary of Traditional Chinese Medicine Prescriptions

Objective:Using data mining method to dig and sort out the prescriptions with Acorus tatarinowiiPolygala tenuifolia drug pair,and summarize the medication characteristics and compatibility of their prescriptions after preliminary screening.Methods:By searching the Dictionary of Traditional Chinese Medicine Prescriptions,a standardized database of prescriptions was established,and the properties,tastes and meridian tropism of prescriptions were classified,and the indications of prescriptions and core combinations of traditional Chinese medicines were analyzed.Results:178 prescriptions were collected,of which 210 were related.Most of the drugs were warm in nature and sweet in taste,and mainly return to the heart and kidney meridians.Under the same confidence and different support,the core drug combination for treating brain diseases was Acorus tatarinowiiPolygala tenuifolia-Ginseng,and the core drug combination for treating asthenia was Acorus tatarinowii-Poria cocos-Rehmannia glutinosa-Polygala tenuifolia.Conclusion:The compatibility characteristics of the prescriptions containing Acorus Tatarinowii and Polygala tenuifolia in the Dictionary of Traditional Chinese Medicine Prescriptions are remarkable,which provides reference for scientific guidance of clinical rational drug use and basic research of prescriptions containing Acorus tatarinowii and Polygala tenuifolia.

基于数据挖掘的痛经用药规律研究

目的:基于数据挖掘技术分析《中医方剂大辞典》中治疗痛经方剂的用药规律。方法:根据纳入与排除标准筛选出《中医方剂大辞典》中治疗痛经的方剂,对其进行方药名称规范,运用Excel、IBM SPSS Modeler 18.0、IBM SPSS Statistics 26.0等进行频数统计、关联规则分析、聚类分析及因子分析。结果:共纳入151首治疗痛经的方剂,涉及中药191味;其中出现频次≥10的中药为当归、川芎、延胡索、白芍、香附、甘草等共36味,药效以补虚药、活血化瘀药、理气药为主,药性偏温、寒、平,药味则偏辛、苦、甘,多入肝、脾、心经。选取出现频次≥25的13味高频药物进行关联规则分析,得到“当归-川芎“”当归-香附-川芎“”当归-熟地黄-白芍-川芎”等常用药对21组,并通过提升关联规则的支持度得到“当归-川芎-白芍”核心组合。进行高频药物聚类分析得出4类药物组合,C1:白芍、熟地黄、川芎、当归;C2:牡丹皮、茯苓;C3:香附、甘草;C4:桃仁、红花,因子分析得到5个公因子。结论《:中医方剂大辞典》中治疗痛经以补血活血、化瘀散结、疏肝理气为主。

Network meta-analysis of the compensatory effect of different Chinese herbal compounds combined with Entecavir in the treatment of hepatitis B cirrhosis

Objective:To evaluate the efficacy and safety of different Chinese herbal compounds combined with Entecavir in the treatment of hepatitis B cirrhosis during the compensatory period by using mesh meta-analysis.Methods:PubMed,CNKI,Wanfang and VIP databases were searched by computer,and the retrieval time was from the establishment of each database to October 5,2022.According to inclusion and exclusion criteria,literature search was conducted independently by two researchers.RevMan5.4.1 software provided by Cochrane was used for evaluation,and Stata16.0 software was used for statistical analysis.Results:A total of 34 RCTs were included,involving 16 TCM compounds and 1543 patients.The results of network meta-analysis showed that ALT indexes of liver function were listed as Yiqi Jiedu Tongluo Method>Luoshugan Tablet>Anluo Huaxian Wan>Qishenrugan Capsule>Qingganhuaji Decoction>Ganshuang Granules>Compound Biejia Rugan Tablet>Rougan Sanjie Decoction>Shugan Jianpi Decoction>Shenqi Fuzheng Huayu Decoction>Peituhua Decoction>Shugan Jianpi Huoxu prescription>Rhubarb Zhezhan Capsule combined with Entecavir treatment respectively;The order of HA index of liver fibrosis was Heluo Shugan Tablet>Shugan Jianpi Huoxui prescription>Anluo Huaxian Wan>Compound Biejia Ruangan Tablet>Rougan Sanjie Decoction>Ganshuang Granules>Danji Huoxui Decoction>Yiqi Jiedu Tongluo Method>Rhubarb Zhezhe Capsule>Fuzheng Huayu Table>Shugan Jianpi Decoction>Rougan Huayu Decoction>Peitu Huayu Decoction>Qingganhuaji Prescription>Shenqi Fuzheng Huayu prescription combined with Entecavir respectively;In order of adverse reactions from best to worst,Shuganjianpi Decoction,Qishenrugangan Capsule,Ganshuang Granules,Peituhuazhi Decoction,compound Biejiruganpian,and He Shugan Pian combined with entecavir,respectively;The effective rate of treatment was listed as Ganshuang Granules>Compound Biejia Ruangan Tablets>uoshugan Tablets>Rougansanjie Decoction>Rhubarb Zhezhe Capsules>Yiqi Jiedu Tongluo Method>Qingganhuaji prescription>Anluo Huaxia Wan>Shugan Jianpi Decoction>Fuzheng Huayu tablets>Peituhuazhi Decoction>Shenqi Fuzheng Huayu prescription combined with Entecavir respectively.Conclusion:Entecavir combined with supplementing qi and detoxifying and dredging collages is the best method to recover ALT index of liver function during the compensation period for hepatitis B cirrhosis;Entecavir combination and Luoshugan tablet were the best treatment for HA index of hepatic fibrosis;Entecavir combined with Shuganjianpi Decoction was the best treatment for adverse reactions;The best treatment efficiency was Entecavir combined with Ganshuang granules.

补肾健脾通络方治疗老年衰弱综合征临床疗效观察

目的观察补肾健脾通络方治疗老年衰弱综合征临床疗效及安全性。方法研究纳入2021年1月—2022年6月收治的老年衰弱综合征患者共计102例,以随机数字表法将患者分为对照组与观察组,每组51例,对照组患者采取临床常规治疗,观察组患者采取常规治疗结合补肾健脾通络方治疗。观察各组数据:治疗总有效率、治疗前后患者中医证候总积分变化及Fried表型衰弱量表(frailty phenotype,FP)评分变化及白介素-6(interleukin 6,IL-6)、超敏C反应蛋白(C-ReactiveProtein,hs-CRP)水平变化、握力变化及起立-行走计时测试(Timed Up and Go test,TUG)变化、5次坐立试验(five-times-sit-to-stand test,FTSST)变化、步速变化、体质指数(Body Mass Index,BMI)变化及四肢骨骼肌质量指数(appendicular skeletal muscle mass index,ASMI)变化、简易精神状态评分(Mini-mental State Examination,MMSE)变化、匹兹堡睡眠指数(Pittsburgh sleep quality index,PSQI)评分变化、日常生活能力(Activity of Daily Living,ADL)评分变化、微型营养评估(mini nutritionaal assessment,MNA)评分变化、患者治疗满意度、不良反应。结果观察组患者治疗总有效率比对照组高[96.08%(49/51)vs84.31%(43/51)],P<0.05;治疗前,各组患者中医证候总积分、FP评分、IL-6、hs-CRP水平、握力、TUG测试结果及FTSST测试结果、步速、BMI、ASMI、MMSE评分、PSQI评分、ADL评分、MNA评分比较,P>0.05,治疗后各组患者中医证候总积分、FP评分、IL-6、hs-CRP水平、握力、TUG测试结果及FTSST测试结果、步速、BMI、ASMI、MMSE评分、PSQI评分、ADL评分、MNA评分均改善,观察组患者治疗后中医证候总积分、FP评分、IL-6、hs-CRP水平、握力、TUG测试结果及FTSST测试结果、步速、BMI、ASMI、MMSE评分、PSQI评分、ADL评分、MNA评分均优于对照组患者,P<0.05;观察组患者治疗满意度高于对照组[98.04%(50/51)vs84.31%(43/51)],P<0.05;观察组与对照组不良反应率均较低,且可自行缓解,P>0.05。结论补肾健脾通络方治疗老年衰弱综合征临床疗效显著,能缓解衰弱综合征患者的临床症状,抑制患者机体炎性反应,增加肌肉质量,改善睡眠,提高生活质量,且无不良反应,较为安全可靠,值得应用。

基于数据挖掘探析国家专利中药复方治疗PACG的用药规律

目的基于数据挖掘探析国家专利中药复方治疗原发性闭角型青光眼(PACG)的用药规律。方法纳入建库至2022年12月31日中国专利公布公告网站中治疗PACG的中药复方专利。采用Excel软件对中药复方建立数据库并进行数据处理,运用R语言进行频数统计、功效归类、性味归经、关联规则及聚类分析。结果(1)一般情况:共纳入专利160项,共涉及药物510味,药物累计频次2,055次。(2)性味归经:四气为寒性药最多,共27味(49.09%);五味以甘味药最多,共71味(47.97%);归经以肝经最多,共38味(25.00%)。(3)高频中药使用频次:使用频次>10次的中药(高频中药)有55味,使用频次最高为枸杞子(46次,28.75%),其次为熟地黄(42次,26.25%)、菊花(41次,25.63%)、甘草(39次,24.38%)、决明子(38次,23.75%)。(4)高频中药功效归类分析:排名前5位为清热药(14味,25.45%)、补虚药(12味,21.82%)、解表药(8味,14.55%)、平肝息风药(5味,9.09%)、利水渗湿药(4味,7.27%)。(5)关联规则分析:高频药对排名前5位分别为:菊花-枸杞子(21次,9.21%)、茯苓-枸杞子(20次,8.77%)、决明子-菊花(20次,8.77%)、泽泻-菊花(18次,7.89%)、熟地黄-菊花(18次,7.89%)。(6)聚类分析:共得到6类有效组合。第Ⅰ类功效为清热泻火,疏肝解郁;第Ⅱ类功效为祛风解表,凉血解毒;第Ⅲ类功效为活血祛瘀,通经止痛;第Ⅳ类功效为滋肾养阴,平肝潜阳;第Ⅴ类功效为滋补肝肾,化痰泻火;第Ⅵ类功效为滋补肝肾,祛风化火。结论国家专利中药复方治疗PACG有规律可循,多从肝、肾、脾入手,组方寒温并用,虚实兼顾,标本兼治,主要针对风、火、痰、虚、郁、瘀六类致病因素。

基于网络药理学的中药组方治疗骨质疏松症的用药规律研究

目的 分析国家专利中药组方治疗骨质疏松症的用药规律并进行网络药理学分析。方法 在国家知识产权局网站中检索治疗慢骨质疏松的中药组方,规范药名后进行中药频数分析、性味归经分析,组方规律分析,药物靶点频数分析,k-means降维PPI分析、GO-BP生物学功能富集分析和KEGGPathway分析。结果 共纳入112项专利中药组方,涉及156味中药,其中淫羊藿的使用频次最高。药性以温性、平性、寒常见;五味多为甘、苦、辛;归经多归属肝经、肾经、脾经。关联规则分析提示常用的药对组合为牡蛎—淫羊藿,三七—当归,黄芪—淫羊藿等。核心靶点主要是AR、ESR1、ESR2、NR1I2、SHBG、VDR、NR3C1,GABRA家族和GABRG家族等。k-mean聚类分析PPI互作网络可降维成4类,与临床分型密切相关。GO和KEGG-Pathway分析表明中药复方主要通过神经活性配体-受体相互作用,氯化物跨膜转运,类固醇激素生物合成,碳水化合物的消化和多种物质成瘾的信号通路,通过消化吸收,神经信号和递质传递,类固醇的代谢,氯离子的转运等功能发挥防治骨质疏松症的作用。结论 结果表明中药治疗原发性骨质疏松症有规律可循,为中药治疗骨质疏松提供了理论依据,为中药的临床研究和基础研究提供了新的思路。

基于数据挖掘的国家专利复方治疗进展期胃癌用药规律

目的基于国家专利数据分析中药复方治疗进展期胃癌的临床用药规律。方法检索中国国家知识产权局专利检索及分析系统建库至2022年11月9日中药复方治疗胃癌公开专利,使用Excel2019、R4.1.2进行药物频次统计、性味归经分析、关联规则分析及聚类分析。结果共纳入专利280项,中药处方280首。高频药物包含甘草、白花蛇舌草、黄芪、茯苓、半枝莲、半夏、当归等,药性以寒、温为主,药味以苦、甘、辛味为主,有毒中药占8.97%,药物归经以肝经、肺经、胃经为主。强关联药物组合有党参+黄芪→白花蛇舌草,茯苓+甘草→白术,党参+甘草→白术,白术+茯苓→甘草等。核心组方为党参、黄芪、白术、茯苓、甘草、半枝莲、白花蛇舌草。聚类分析获得5类药物组合。结论国家专利复方治疗进展期胃癌核心处方以扶正祛邪为主要原则,扶正常用党参、白术、黄芪、茯苓、甘草等,祛邪常用半枝莲、白花蛇舌草、猕猴桃根等,针对血虚、血瘀、湿热、痰凝等不同辨证可选白芍、莪术、三七、延胡索、薏苡仁、半夏等药物。

基于大语言预训练模型的中医个性化处方推荐研究

目的针对中医个性化处方推荐问题,研究自动化处方推荐任务,为中医临床辅助决策提供参考。方法基于大语言预训练文本生成模型设计一种中医个性化处方推荐算法。将中医处方推荐任务转化为端到端(seq2seq)的文本生成任务,即将临床症状描述文本通过模型转化为处方文本,以实现处方推荐任务的需求,并利用基于大语言预训练的BART(Bidirectional and Auto-Regressive Transformers)模型的预训练参数来提升模型对通用语义信息的理解,通过对训练集处方内中药排序提升模型的处方推荐性能。结果实验证明通过大语言预训练模型以及端到端的文本生成架构可有效提升模型的生成性能,同时对处方内中药依次排序可以获取更高准确率,并且通过中药的排列获取更多值得参考的有价值信息。中医个性化处方推荐模型在处方排序后分别在前5、10、15味生成的处方分别取得了58.60、53.79和49.67的准确率。结论中医个性化处方推荐模型取得了更优的处方推荐效果,表明其可为中医临床治疗疾病进行参考,达到辅助临床决策支持的效果。

基于国家专利的中药治疗癫痫组方规律数据挖掘研究

目的基于国家专利探析中药治疗癫痫的组方规律,为临床治疗提供参考。方法搜集中国专利公布公告网建库至2023年8月21日符合纳入标准的中药处方治疗癫痫的专利数据,通过Excel2021建立处方药物数据库,采用Python、SPSS Modeler 18.0、Cytoscape3.10.1、R语言软件进行频次统计、数据建模、复杂网络分析、关联规则分析、聚类分析,探究组方规律。结果纳入符合标准的中药处方治疗癫痫专利数据376项,涉及中药827味,使用总频次为4911;用药频次前5位为天麻、石菖蒲、僵蚕、全蝎、钩藤;高频药物功效类别以平肝息风药、安神药、化痰止咳平喘药为主,药性以平、温、寒为主,药味以甘、辛、苦为主,归经以肝经、心经、肺经、脾经为主;中药核心复杂网络筛选得到核心组合天麻-石菖蒲-僵蚕。关联规则分析得到常用药物组合28条,其中支持度最高为全蝎-僵蚕。聚类分析得到4条数据。相似性分析得出专利复方治疗癫痫具有独特性。结论中药处方治疗癫痫国家专利的高频药物及常用药物组合以息风止痉、豁痰开窍为主,可为临床提供参考。

应用关联规则和因子分析寻找中药治疗良性前列腺增生的用药规律

目的基于数据挖掘技术分析中医复方治疗中老年良性前列腺增生(BPH)的用药规律。方法检索PubMed、中国知网、维普、万方、中国生物医学文献、Embase等数据库2012年1月至2022年12月中药复方治疗BPH的临床研究文献,总结中药的使用频率、类别、功效、四气、五味及经络,运用SPSS Modeler 18.0关联规则和因子分析发掘深藏的中药搭配。结果按标准挑选153篇文献,纳入173味中药,频次较高的有黄芪、茯苓、桃仁、肉桂、泽泻、山茱萸等,以平、温药性为主,主要是甘、苦、辛药味,归肝经为主药,功效以利尿通淋为主,其次为利水消肿、润肠通便等。通过聚类分析形成了6个聚类组;关联分析中,二联支持度最高的是黄柏和知母、三棱和莪术的药对组合;三联关联中山药、附子、牡丹皮等7种组合具有最佳置信度。因子分析中提炼到8个公因子。结论中药治疗BPH具有较好的临床效果,聚类分析和因子分析可用于指导临床更加安全合理用药。

补虚化瘀组方治疗原发性胆汁性胆管炎的效果

目的探究补虚化瘀组方对原发性胆汁性胆管炎(primary biliary cholangitis,PBC)中医临床症状及免疫功能的影响。方法选取80例PBC患者作为研究对象,用单双球法随机分为对照组和治疗组,每组40例。对照组给予基础治疗,治疗组在基础治疗的基础上予以补虚化瘀组方治疗。比较治疗前和治疗6个月后,2组患者中医症候积分(腹胀、胁肋疼痛、乏力、皮肤瘙痒及大便不调)、肝功能指标[天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、谷氨酰转肽酶(glutamyl transpeptidase,GGT)、碱性磷酸酶(alkaline phosphatase,ALP)及总胆红素(total bilirubin,TB)]、免疫功能[免疫细胞(CD4^(+)、CD8^(+))、免疫球蛋白A(immunoglobulin A,IgA)、免疫球蛋白G(immunoglobulin G,IgG)及免疫球蛋白M(immunoglobulin M,IgM)],记录2组患者不良反应(头晕、恶心呕吐、腹泻及皮疹)的发生情况。结果治疗6个月后,2组患者的中医症候积分、肝功能指标及免疫功能均降低,且治疗组低于对照组(均P<0.05);6个月内,治疗组熊去氧胆酸(ursodeoxycholic acid,UDCA)应答率高于对照组(P<0.05),2组患者总不良反应发生率比较差异无统计学意义(P>0.05)。结论补虚化瘀组方可以改善PBC患者中医临床症状及肝功能,增强患者免疫力,减少不良反应的发生。

基于《中医方剂大辞典》的雷丸组方用药规律分析

目的 基于数据挖掘方法探究《中医方剂大辞典》中雷丸的组方用药规律。方法 以《中医方剂大辞典》为信息源,梳理含雷丸的方剂,构建雷丸方药数据库;采用文献分析和数据挖掘方法,探讨雷丸的增效减毒及其方剂主治病症类型、药物配伍情况;利用关联规则分析探寻其在常见病中的配伍特点;使用皮尔逊相关性分析发掘药-证的相关性,总结其用药规律。结果 含有雷丸方剂的剂型主要为丸剂和散剂,高频用药为槟榔、大黄、木香等,与雷丸配伍的主要为驱虫药、泻下药及理气药等,且味多为苦、辛,归胃经、脾经。其中“槟榔-雷丸”出现的药对配伍频数最高,其次是“大黄-雷丸”“木香-雷丸”;雷丸方剂的主治病症为虫积的频数最高,其次为疳证、积滞等。皮尔逊相关性分析结果表明,治疗虫积配伍槟榔、木香、使君子、芜荑、鹤虱等;治疗疳证配伍胡黄连、黄连、青皮等;治疗积滞配伍神曲、陈皮、牵牛子、三棱等。结论 雷丸与槟榔等配伍可加强杀虫消积的功效;与胡黄连、大黄等配伍可增加泻下攻积的作用,以上可能会引起轻微的胃肠道不良反应;与陈皮、神曲等配伍可保护胃黏膜,减轻胃肠不良反应。但雷丸毒性物质基础研究等未见报道,仍需深入研究,为雷丸临床应用提供更确切的基础。