Bone tissue with strong adaptability is often in a complex dynamical microenvironment in vivo,which is associated with the pathogenesis and treatment of orthopedic diseases.Therefore,it is of great significance to inv...Bone tissue with strong adaptability is often in a complex dynamical microenvironment in vivo,which is associated with the pathogenesis and treatment of orthopedic diseases.Therefore,it is of great significance to investigate the effects of corresponding compound stimulation on cell behaviors.Herein,a fluid shear stress(FSS)plus ultrasound stimulation platform suitable for cell studies based on a microfluidic chip was constructed and bone marrow mesenchymal stem cell(BMSC)was chosen as a model cell.The proliferation and osteogenesis of BMSCs under the compound stimulation of FSS plus ultrasound in growth medium without any soluble induction factors were firstly investigated.Single FSS stimulation and static culture conditions were also examined.Results illustrated that suitable single FSS stimulation(about 0.06 dyn/cm^(2))could significantly enhance cell proliferation and osteogenesis simultaneously when compared to the static control,while greater FSS mitigated or even restricted these enhancing effects.Interestingly,ultrasound stimulation combined with this suitable FSS stimulation further accelerated cell proliferation as the intensity of ultrasound increasing.As for the osteogenesis under compound stimulation,it was relatively restricted under lower ultrasound intensity(about 0.075W/cm^(2)),while promoted when the intensity became higher(about 1.75W/cm^(2)).This study suggests that both the cell proliferation and osteogenesis are very responsive to the magnitudes of FSS and ultrasound stimulations and can be both significantly enhanced by proper combination strategies.Moreover,these findings will provide valuable references for the construction of effective cell bioreactors and also the treatment of orthopedic diseases.展开更多
基金supported by the National Key Research and Development Program of China(2018YFC1105800,2018YFC1105801)the Program of Shanghai Academic/Technology Research Leader(20XD1400100)+4 种基金the Natural Science Foundation of Shanghai(20ZR1402400)the National Natural Science Foundation of China(51703033,52173031,51903045)the Basic Research Project of the Science and Technology Commission of Shanghai Municipality(21JC1400100)the International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality(19520744500)the Fundamental Research Funds for the Central Universities(2232020D-04,2232019A3-06,2232019D3-02).
文摘Bone tissue with strong adaptability is often in a complex dynamical microenvironment in vivo,which is associated with the pathogenesis and treatment of orthopedic diseases.Therefore,it is of great significance to investigate the effects of corresponding compound stimulation on cell behaviors.Herein,a fluid shear stress(FSS)plus ultrasound stimulation platform suitable for cell studies based on a microfluidic chip was constructed and bone marrow mesenchymal stem cell(BMSC)was chosen as a model cell.The proliferation and osteogenesis of BMSCs under the compound stimulation of FSS plus ultrasound in growth medium without any soluble induction factors were firstly investigated.Single FSS stimulation and static culture conditions were also examined.Results illustrated that suitable single FSS stimulation(about 0.06 dyn/cm^(2))could significantly enhance cell proliferation and osteogenesis simultaneously when compared to the static control,while greater FSS mitigated or even restricted these enhancing effects.Interestingly,ultrasound stimulation combined with this suitable FSS stimulation further accelerated cell proliferation as the intensity of ultrasound increasing.As for the osteogenesis under compound stimulation,it was relatively restricted under lower ultrasound intensity(about 0.075W/cm^(2)),while promoted when the intensity became higher(about 1.75W/cm^(2)).This study suggests that both the cell proliferation and osteogenesis are very responsive to the magnitudes of FSS and ultrasound stimulations and can be both significantly enhanced by proper combination strategies.Moreover,these findings will provide valuable references for the construction of effective cell bioreactors and also the treatment of orthopedic diseases.
