Compound 3k治疗骨关节炎:调控氧化应激通路改善软骨细胞糖酵解的作用机制

背景:骨关节炎现已被认为是一种代谢性疾病,既往研究表明糖酵解在骨关节炎的发生发展中起重要作用。Compound 3k作为一种新型糖酵解小分子抑制剂,具有抗炎及抗肿瘤等功效,因此可靶向糖酵解,有望为骨关节炎治疗提供新的思路。目的:基于缺氧诱导因子1α/活性氧的氧化应激通路探究Compound 3k在糖酵解过度活跃所导致的骨关节炎中的作用机制。方法:取对数生长期的ATDC5成软骨细胞,用10 ng/mL白细胞介素1β作用24 h诱导骨关节炎体外细胞模型,以CCK-8法检测不同浓度(0.25,0.5,1,2.5,5,10,15μmol/L)Compound 3k的细胞毒性,选出合适浓度进行后续实验。将软骨细胞随机分为对照组、模型组、治疗组,模型组以10 ng/mL的白细胞介素1β诱导,治疗组以Compound 3k预刺激2 h后与白细胞介素1β共培养,用CCK-8法检测各组细胞增殖情况;用ELISA试剂盒检测各组细胞炎症水平;用试剂盒检测各组细胞活性氧、细胞外乳酸脱氢酶及葡萄糖含量;qRT-PCR及Western blot检测相关炎症因子白细胞介素6、肿瘤坏死因子α及糖酵解相关基因葡萄糖转运蛋白1、甘油醛3-磷酸脱氢酶、单羧酸转运蛋白1和缺氧诱导因子1α的表达水平。结果与结论:①与对照组相比,模型组细胞增殖活性下降、糖酵解水平活跃,表现为细胞外乳酸脱氢酶含量增加(P<0.001),葡萄糖含量减少(P<0.001),相关炎症因子白细胞介素6(P<0.0001)及肿瘤坏死因子α(P<0.001),糖酵解相关基因葡萄糖转运蛋白1(P<0.001)、甘油醛3-磷酸脱氢酶(P<0.001)、单羧酸转运蛋白1(P<0.001)及缺氧诱导因子1α(P<0.001)的表达水平均上调,并伴随氧化应激,活性氧过量产生。②与模型组相比,Compound 3k的治疗有效提高细胞增殖活性,抑制过度活跃的糖酵解水平的同时,抑制了骨关节炎软骨细胞炎症(P<0.001)及糖酵解相关基因的表达(P<0.001),且抑制氧化应激,缺氧诱导因子1α的表达水平下调(P<0.0001),活性氧水平下降。③上述结果证实,Compound 3k抑制了白细胞介素1β诱导的软骨细胞炎症,其机制可能与糖酵解及缺氧诱导因子1α/活性氧介导的氧化应激有关。

STRESS ANALYSIS OF THE APERTURE IN COMPOUND PIPELINE BY FEM

There is a cyclical aperture at a certain part of the fiberglass reinforced plastic compound pipeline with a stainless steel pipe as a liner, which becomes the weakness of the conduit. The field of stress and strain of the aperture was investigated using the nonliner finite element code Marc. The results of the finite element analysis showed that there were concentrations of stress and strain in the layer of stainless steel at the part of the pipeline where the aperture existed. The method to solve these problems was also discussed.

An Overview on Exogenous Compounds That Mitigate Heat Stress in Crops

With the global warming and climate change in recent years, heat stress has become one of the major abiotic factors constraining production and productivity of crops in many regions around the world. Although the plants have developed some adaptive behaviors and avoidance mechanisms against heat stress, they will be hurt under extreme high temperature. Exogenous compounds could enhance plant growth and development, antioxidant enzymes activities, chlorophyll and nutrient contents under stress conditions. Meanwhile, application of exogenous compounds sustains physiological and biochemical process and prevents plants from physiological changes under stress conditions. In this review, effects of heat stress upon crops, and the functions of hormones in plant adaptation to heat stress are simply stated. Then, we intend to appraise how various phytohomrnones that are related to plant adaptation to heat stress. The role of abscisic acid(ABA), salicylic acid(SA) and polyamines(putrescine and spermine) under heat stress conditions are mainly discussed.

STRESS ANALYSIS OF CANNED HOT FORGING SPECIMEN OF TiAl INTERMETALLIC COMPOUND

ＳＴＲＥＳＳＡＮＡＬＹＳＩＳＯＦＣＡＮＮＥＤＨＯＴＦＯＲＧＩＮＧＳＰＥＣＩＭＥＮＯＦＴｉＡｌＩＮＴＥＲＭＥＴＡＬＬＩＣＣＯＭＰＯＵＮＤ①ＲａｏＱｉｕｈｕａＤｅｐａｒｔｍｅｎｔｏｆＡｒｃｈｉｔｅｃｔｕｒｅＥｎｇｉｎｅｒｉｎｇ，ＨｅＹｅｈｕｉ，Ｈｕａｎｇ...

The Protective Effect of Compound Danshen Dripping Pills on Oxidative Stress after Retinal Ischemia/Reperfusion Injury in Rats

Objective: To investigate the effect of Compound Danshen Dripping Pills (CDDP) on oxidative stress after ischemia/reperfusion (I/R) injury in the rat retina. Methods: Adult male SD rats were randomly divided into 3 groups: sham (group A), I/R (group B), and I/R plus CDDP (group C). Retinal ischemia/reperfusion injury (RIRI) was introduced by increasing the intraocular pressure (IOP) to 110 mmHg for 60 min via cannulation into the anterior chamber. Right after the insult, CDDP was administered intragastrically (450 mg/kg/d) for 7 days. The levels of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in the retinal tissues were determined on d1 and d7 after the ischemic insult. Results: Following ischemia, the MDA levels in group B and group C were significantly higher than those in group A (p < 0.01). CDDP significantly lowered MDA levels in group C when compared with group B (p < 0.01). The activities of SOD, GSH-Px and CAT were higher in group A than in group B and group C (p < 0.01). CDDP could increase the activities of SOD, GSH-Px and CAT remarkably in group C when compared with group B (p < 0.01). Conclusion: CDDP can protect the retina from I/R injury through reducing oxidative stress, and thus may be a promising method for the treatment of ischemic retinal disorders.

天然产物调控氧化应激治疗脊髓损伤

背景:脊髓损伤是一种严重的神经系统疾病,常导致神经功能严重受损。在脊髓损伤后的病理过程中,氧化应激是一个重要环节,导致神经细胞死亡和功能丧失。近年来,天然产物因来源广泛、结构多样且生物活性丰富,逐渐在脊髓损伤后的氧化应激治疗中展现出潜在的应用价值。目的:讨论部分天然产物在脊髓损伤后氧化应激过程中的治疗作用以及相关作用机制,以期为脊髓损伤的抗氧化治疗提供新的思路和方向。方法:以“spinal cord injury,oxidative stress,anti-oxidation,natural products,natural compounds,polyphenols”“脊髓损伤,氧化应激,抗氧化,天然产物,天然化合物,多酚”为关键词,在PubMed、Web of Science、Embase、Cochrane、维普、CBM、万方和中国知网数据库检索自建库以来至2024年5月的相关文献。制定纳入和排除标准,通过阅读文献标题、摘要及全文内容进行筛选,最终纳入97篇相关文献。结果与结论:①天然产物如多酚类等可以依靠其结构中的酚羟基发挥直接清除氧化自由基的作用,减轻脊髓损伤后的氧化应激反应;②一些天然产物可以通过调控某些信号转导通路增强体内相关抗氧化酶的活性,减轻氧化应激;③部分天然产物可以通过增强自噬的方式减弱脊髓损伤后的氧化应激;④利用天然产物调控氧化应激可能成为今后临床上治疗脊髓损伤的有效工具。

Plant Senescence: The Role of Volatile Terpene Compounds (VTCs)

Senescence is a natural, energy-dependent, physiological, developmental and an ecological process that is controlled by the plant’s own genetic program, allowing maximum recovery of nutrients from older organs for the survival of the plant, as such;it is classified as essential component of the growth and development of plants. In some cases, under one or many environmental stresses, senescence is triggered in plants. Despite many studies in the area, less consideration has been given to plant secondary metabolites, especially the role of VTCs on plant senescence. This review seeks to capture the biosynthesis and signal transduction of VTCs, the physiology of VTCs in plant development and how that is linked to some phytohormones to induce senescence. Much progress has been made in the elucidation of metabolic pathways leading to the biosynthesis of VTCs. In addition to the classical cytosolic mevalonic acid (MVA) pathway from acetyl-CoA, the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, originating from glyceraldehyde-3-phosphate (GAP) and pyruvate, leads to the biosynthesis of isoprenoid precursors, isopentenyl diphosphate and dimethyl allyl diphosphate. VTCs synthesis and emission are believed to be tightly regulated by photosynthetic carbon supply into MEP pathway. Thus, under abiotic stresses such as drought, high salinity, high and low temperature, and low CO2 that directly affect stomatal conductance and ultimately biochemical limitation to photosynthesis, there has been observed induction of VTC synthesis and emissions, reflecting the elicitation of MEP pathway. This reveals the possibility of important function(s) of VTCs in plant defense against stress by mobilizing resources from components of plants and therefore, senescence. Our current understanding of the relationship between environmental responses and senescence mostly comes from the study of senescence response to phytohormones such as abscisic acid, jasmonic acid, ethylene and salicylic acid, which are extensively involved in response to various abiotic and biotic stresses. These stresses affect synthesis and/or signaling pathways of phytohormones to eventually trigger expression of stress-responsive genes, which in turn appears to affect leaf senescence. Comparison of plant response to stresses in relation to patterns of VTCs and phytohormones biosynthesis indicates a considerable crosstalk between these metabolic processes and their signal to plant senescence.

Metabolomics Analysis of Tall Fescue Leaves under Drought Stress

Abstract The metabolites of tall fescue leaves under normal and drought conditions were analyzed with LC-ESI-MS （ liquid chromatography tandem mass spec- trometry）. A total of 282 metabolites were stably detected in the two groups using OPI.S-DA （orthogonal partial least squares discriminant analysis） method; 148 of which were down-regulated while 134 were up-regulated. After analyzed with MZ-minc software, 10 major metabolites （4 down, 6 up） were identified through searehing public database. Aromatic compounds, 3-p-eoumaroyll, 5-quinolaetone, aromatic free amino acids, styraxin mad methanofuran, were up-regulated ; grease metabolites, methyl 15-hydroperoxy-9Z, 12Z, 16E-octadecatrienoate, 9,12,13-Tri-HOME （10）, 3-oxo-dodecanoic acid, and 13 （S）-HpOTrE acid ester, were down-regulated; piperanine （alkaloids） was down-regulated while Methyl 7-epi-12-hydroxyjasmonate glucoside （indicant） was up-regulated. In a word, aromatic compounds and liquid metabolites may play vital roles in drought-resistant regulation of tall fescue.

The Influence of Oral Malodor on Psychological Stress

We examined the influence of oral malodor on psychological stress among patients with a chief complaint of oral malodor. A total of 101 patients (40 male and 61 female) who visited our clinic with a chief complaint of oral malodor were included in this study. The primary compounds of oral malodor—volatile sulfur compounds (VSC)—were measured using the Oral ChromaTM instrument. Salivary stress markers, cortisol, and chromogranin A were measured using an enzyme-linked immunosorbent assay (ELISA) kit. The mood was measured using the Profile of Mood States 2nd Edition (POMS2). Among the participants, 60.3% had at least one level above the threshold in the following three markers: hydrogen sulfide, methyl mercaptan, and dimethyl sulfide. The gender distribution was 35 female (57.4%) and 25 male (62.5%). Salivary cortisol levels, a biomarker of stress, did not vary depending on the presence or absence of oral malodor. Chromogranin A levels were higher in the oral malodor female group than in the no oral malodor female group. With respect to the POMS2, regardless of the presence or absence of oral malodor measured using gas chromatography, the participants had higher scores for “Anger-Hostility” and “Fatigue-Inertia”. Analysis by gender showed that “Depression-Dejection” was significantly higher in the oral malodor female group than in the no oral malodor female group. These results suggest that those who are worried about oral malodor have higher psychological stress than those who are not, regardless of the presence or absence of oral malodor measured using gas chromatography.

Hydraulic calculation of steady uniform flows in trapezoidal compound open channels

Hydraulic calculation of steady uniform flows in trapezoidal compound open channels is studied. Based on the force balance of water in each sub-section, the average velocities of the main channel, side slope, and floodplain are derived. The lateral momentum exchanges between the sub-sections are expressed by using the apparent shear stress. To verify the model, seven groups of UK Flood Channel Facility （UK-FCF） measured data with a relative water depth between the floodplain and the main channel varying from 0.057 to 0.4 are used for comparison. The result shows that the calculated velocity is larger than the measured data when the relative water depth is small, while it is less than or close to the measured value in the case of a larger relative water depth. The influence of the apparent shear stress on the calculation of velocity on the floodplain is not obvious, while it is much greater on the main channel. The three-stage model is compared with Liu’s two-stage model, showing that the former can give a better prediction for a three-stage trapezoidal compound channel. Finally, the apparent shear stress is calculated and compared with the measured data. The result shows that the chosen values of the momentum transfer coefficients are appropriate.

土壤Cd、Pb污染对苣苋草生物量、生理生化特征和Cd、Pb积累特性的影响

【目的】比较两品种苣苋草对Cd、Pb胁迫的生理生化响应的差异性,寻找对Cd、Pb胁迫抗性较强的品种。【方法】采用盆栽试验,研究不同浓度Cd、Pb单一及复合胁迫下‘R104’和‘K472’两品种苣苋草的生物量,Cd、Pb积累特性,渗透调节物质含量和抗氧化酶活性的变化。【结果】两品种苣苋草的生物量在单一Cd胁迫时下降,在低浓度Pb(2.5 g·kg^(-1))胁迫时上升,在高浓度Pb(10.0 g·kg^(-1))胁迫时下降,在Cd、Pb复合胁迫时表现为先上升后下降的趋势。Cd、Pb单一胁迫下,苣苋草的Cd、Pb含量随着相应元素胁迫浓度的增大而上升;Cd、Pb复合胁迫下,苣苋草的Cd、Pb含量随着另一元素胁迫浓度的增大呈先上升后下降的趋势。随着Cd、Pb单一及复合胁迫浓度的增大,苣苋草超氧化物歧化酶(SOD)活性呈先上升后下降的趋势,过氧化物酶(POD)活性逐渐上升,过氧化氢酶(CAT)活性呈抑制效应。Cd、Pb交互作用在苣苋草生物量和SOD活性上总体表现为复合胁迫>单一胁迫;在植物重金属含量上表现为:低浓度Cd与Pb互为促进作用,高浓度Cd与Pb互为拮抗作用。【结论】苣苋草主要通过提高POD活性和维持CAT活性来抵抗Cd、Pb胁迫;‘R104’的抗性大于‘K472’,表明‘R104’更适合在重金属Pb污染的土壤区域推广种植。

Stress distribution at diffusion-bonded interface of Fe_3Al with Cr18-Ni8 steel

Fe3Al and Crl8-Ni8 steel were bonded in vacuum and an interface was formed between Fe3Al and Crl8-Ni8 steel. Stress distribution at the diffusion-bonded interface was researched by numerical simulation and finite element method （FEM）. The results indicated that the peak stress appeared at the interface near Cr18-Ni8 steel side. This is the key factor to induce crack at this position. With the enhancement of heating temperature, the peak stress at the bonded interface increases. When the temperature is 1 100 22, the peak stress is up to 65.9 MPa, which is bigger than that at 1 000 22 by 9. 4%. In addition, the peak stress becomes bigger with the increase of the thickness of base metal from 1 mm to 8 ram. While the thickness is more than 8 ram, the peak stress varies slightly with the change of the thickness.

African eggplant(Solanum anguivi Lam.)fruit with bioactive polyphenolic compounds exerts in vitro antioxidant properties and inhibits Ca^(2+)-induced mitochondrial swelling

Objective:To evaluate the antioxidant and radical scavenging activities of Solanum anguivi fruit(SAG)and its possible effect on mitochondrial permeability transition pore as well as mitochondrial membrane potential(△Ψ_m)isolated from rat liver.Methods:Antioxidant activity of SAG was assayed by using 2,2-diphenyl-1-picrylhydrazyl(DPPH),reducing power,iron chelation and ability to inhibit lipid peroxidation in both liver and brain homogenate of rats.Also,the effect of SAG on mitochondrial membrane potential and mitochondrial swelling were determined.Identification and quantification of bioactive polyphenolics was done by HPLC-DAD.Results:SAG exhibited potent and concentration dependent free radical-scavenging activity(IC_(50)/DPPH=275.03±7.8μg/mL).Reductive and iron chelation abilities also increase with increase in SAG concentration.SAG also inhibited peroxidation of cerebral and hepatic lipids subjected to iron oxidative assault.SAG protected against Ca^(2+)(110μmol/L)-induced mitochondrial swelling and maintained the△Ψ_m.HPLC analysis revealed the presence of gallic acid[(17.54±0.04)mg/g],chlorogenic acid(21.90±0.02 mg/g),caffeic acid(16.64±0.01 mg/g).rutin[(14.71±0.03)mg/g]and quercetin[(7.39±0.05)mg/g].Conclusions:These effects could be attributed to the bioactive polyphenolic compounds present in the extract.Our results suggest that SAG extract is a potential source of natural antioxidants that may be used not only in pharmaceutical and food industry but also in the treatment of diseases associated with oxidative stress.

Modeling of Temperature and Residual Stress Fields Resulting from Impacting Process of a Molten Ni Particle onto a Flat Substrate

Several effective numerical techniques,based on a finite element analysis,have been developed and computed independently.Results are presented describing the impacting process,and the subsequent temperature and residual stress fields of a molten nickel particle impacting onto a flat substrate.Problems of this type,especially the prediction of the thermal residual stresses,are of major practical interest in thermal spray operations as a pioneering approach.

Protective Effect of Phenolic-Rich Extracts from Different Parts of <i>Opuntia joconostle</i>Fruit against Carbon Tetrachloride-Induced Oxidative Stress in Mice

Opuntia joconostle fruit is a rich source of biocompounds such as polyphenols including gallic, vanilic, 4-hidroxybenzoic, cafeic, and syringic acids, catechin, epicatechin, rutin, and vanillin, besides betalains. The objective of this research was to evaluate the effect of supplementation polyphenols-rich extracts from different parts of Opuntia joconostle against carbon tetrachloride-induced oxidative stress in a mouse model. The animals were treated orally with polyphenols-rich extracts at 50, 100 and 200 mg/kg BW for 30 consecutive days. On day 30th the mice received carbon tetrachloride (CCl4) as hepatoxic agent. Biochemical evaluations were carried out 24 h after induction of the oxidative stress. Data showed that methanolic extracts from different parts of Opuntia joconostle exerting protective effect against the CCl4-induced oxidative stress in mice. Histology examination revealed that the damage decreased in groups treated with polyphenols-rich extracts compared to the group that did not receive any treatment. Opuntia joconostle fruit contains many phenolic compounds, flavonoids and betalains. The protective effect of extracts may be related to the phenolic composition and also by a counteraction with other compounds, such as betalains and flavonoids that increase their antioxidant effect.

Effect of the Compound Layer of Plasma Nitriding on Thermal Fatigue Behavior of 4Cr5MoSiV1 Die Steel

With the Uddeholm self restricted method, the effect of compound layer of plasma nitriding on thermal fatigue behavior of 4Cr5MoSiV1 steel was studied by the way of adding Ar during plasma nitriding to remove the compound layer. The results show that the compound layer of plasma nitriding can delay the nucleation of heat cracks and hold back the propagation of heat cracks from surface to substrate because of its high hardness and strength. On the other land, the heat checking expands faster with the compound layer on the surface than that without it. After 3000 cycles of thermal fatigue test, both heat cracks with the compound layer are wider than the another without compound layer and the number of heat cracks of the former is more from the view of cross section.

复方中药对热应激条件下肉牛生理指标、生长性能及血清生化指标的影响

试验旨在探讨复方中药对热应激条件下肉牛生理指标、生长性能及血清生化指标的影响。选取体况良好、体重相近的育肥期夏南牛24头,随机分为4组,每组6个重复,每个重复1头牛。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加0.5%、1.0%和1.5%的复方中药。试验期30 d。结果显示:整个试验期间牛舍内环境温湿指数(THI)大于72,牛处于热应激状态。在试验第20、30 d,1.0%组和1.5%组牛的呼吸频率显著低于对照组(P<0.05)。与对照组相比,1.0%组牛的干物质采食量和平均日增重显著升高(P<0.05),0.5%和1.0%组的料重比显著降低(P<0.05);1.0%组的总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)含量、总抗氧化能力(T-AOC)以及谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性显著升高(P<0.05),尿素氮(BUN)、葡萄糖(GLU)和丙二醛(MDA)的含量显著降低(P<0.05);1.0%和1.5%组的免疫球蛋白G (IgG)和白细胞介素-6 (IL-6)含量显著升高(P<0.05)。研究表明,饲粮中添加复方中药可以改善肉牛的热应激反应,添加量以1.0%效果较好。

应力波作用下红砂岩复合动态断裂特征研究

为探究应力波作用下红砂岩的复合动态断裂特性,采用分离式霍普金森压杆和数字图像技术对带预制裂缝的半圆盘三点弯曲试样开展冲击加载实验,分析加载率、波长对红砂岩动态拉伸、断裂特征的影响。结果表明:①红砂岩试样加载率与动态抗拉强度、断裂韧度及破坏率均呈一次函数关系;断裂能随加载率增大而增长415.27%。②随着波长增加,动态抗拉强度增长742.14%,其中断裂能增长54.49%,但能量吸收率呈下降趋势;裂纹扩展平均速度增长4.09%,且首尾裂纹应变增长时间出现滞后现象;破坏率增长效应得到强化。③冲击速度8 m/s时试件主裂纹首尾监测点位的应变平均增加84.31%。

天然产物调控氧化应激治疗骨质疏松症

背景:越来越多的研究表明氧化应激在治疗骨质疏松症方面发挥着重要作用,氧化应激会导致氧化活性物质的累积,进而损伤骨相关细胞,最终引起骨吸收和骨生成的失衡,导致骨量减少以及骨微结构的破坏。近年来研究发现,一些天然产物可以调节氧化应激治疗骨质疏松症,其来源广泛、不良反应小等特点在治疗骨质疏松症方面具有明显的优势,疗效可观。目的:讨论天然产物调控氧化应激治疗骨质疏松症的机制,并根据最新的相关研究进展展开综述,以期为今后更多天然产物治疗骨质疏松症的研究提供参考和思路,并为临床上应用天然化合物治疗骨质疏松症提供数据支持。方法:以“oxidative stress,free radical,antioxidant,phytotherapy,plant extracts,medicinal plants,herbal medicine,osteoporosis,bone density,bone loss”“氧化应激,自由基,抗氧化,天然产物,植物提取物,植物药,中药,骨质疏松症,骨密度,骨丢失”为关键词在PubMed、Web of Science、Embase、Cochrane、维普、CBM、万方和中国知网数据库检索2010年1月至2023年2月的相关文献。制定纳入和排除标准,通过阅读文献标题、摘要及全文内容进行筛选,最终纳入64篇相关文献。结果与结论:①一些天然产物具有抗氧化作用,并可以通过改善氧化应激调控成骨分化、调控成骨细胞骨基质矿化、调控破骨细胞介导的骨吸收、调控骨相关细胞的增殖、分化、活性和凋亡,从而影响骨代谢过程;②这些具有抗氧化作用的天然产物通过改善骨重塑平衡起到治疗骨质疏松症的作用;③多种天然产物联合使用改善骨质疏松症的研究还有待探索;④利用天然产物调控氧化应激可能成为今后临床上治疗骨质疏松症的有力武器。

盐碱复合胁迫对湖南稷子种子萌发的影响

【目的】研究盐碱复合胁迫对湖南稷子种子萌发和幼苗生长的影响。【方法】模拟盐碱地自然状态下盐分组成特点,以2种中性盐(NaCl、Na_(2)SO_(4))和2种碱性盐(NaHCO_(3)、Na_(2)CO_(3))分别按1∶1∶0∶0(A)、1∶2∶1∶0(B)、1∶9∶9∶1(C)、1∶1∶1∶1(D)、9∶1∶1∶9(E)的比例混合,以湖南稷子为材料,去离子水为对照(CK),研究盐碱混合胁迫对其萌发指标及幼苗生长的影响。【结果】湖南稷子种子的萌发率随盐分浓度的升高而明显下降(P<0.05);但是,当盐浓度为150 mmol/L时,湖南稷子种子在第5d的发芽率较对照(97.3%)分别降低了8.5%,16.4%,23.6%,随着盐的浓度增加到250 mmol/L,湖南稷子的萌发率明显降低(P<0.01);湖南稷子的发芽势、发芽指数、活力指数、根长及苗长等指标变化趋势均与发芽率的相吻合。湖南稷子种子在中性和碱性复合胁迫下的萌发受到了较大的抑制,而在250 mmol/L的中性盐复配下,湖南稷子的所有发芽指数都为0。随着盐分的升高,叶片中Na^(+)、脯氨酸、MDA的含量逐渐上升,K^(+)的含量则明显降低。【结论】随着盐胁迫浓度增加,对湖南稷子种子萌发的抑制作用越明显。本研究结果可为盐碱地湖南稷子的种植提供理论基础。