[Objective] The paper was to determine whether the germplasm resources of Congjiang fragrance pig had changed over the past 30 years. [Method]The growth development and reproductive performance of 100 pigs from six to...[Objective] The paper was to determine whether the germplasm resources of Congjiang fragrance pig had changed over the past 30 years. [Method]The growth development and reproductive performance of 100 pigs from six towns in central fragrance pig producing area and Congjiang fragrance pig stock seed farm were measured,and further compared with the data in Guizhou Livestock and Poultry Breeds 1986. [Result]The growth and development status was basically consistent,and the average litter size was at a downward trend: the first litter decreased by 26. 2%; the second litter decreased by 7. 0%; the third litter decreased by8. 55%. [Conclusion] The study laid the foundation for vigorous promotion of Congjiang fragrance pig in industrial development process,acceleration of breeding pace,gradual updating of farming breeds by farmers and improvement of production efficiency of fragrance pig.展开更多
Fragrance pig is a famous miniature local breed in China,which is similar to human on the aspects of physical structure,anatomy,nutrition,metabolism and blood biochemical indicators. The internal organs of Congjiang f...Fragrance pig is a famous miniature local breed in China,which is similar to human on the aspects of physical structure,anatomy,nutrition,metabolism and blood biochemical indicators. The internal organs of Congjiang fragrance pig and Huanjiang fragrance pig with different month ages were weighed. The results showed that the proportion of stomach in body weight in Congjiang fragrance pig was higher than that in Min pig,Harbin white and Landrace,and the proportion of large intestine in body weight in Congjiang fragrance pig was also higher than that in ordinary pigs; the weights of heart,liver and kidney in 8- 10 months old Congjiang fragrance pig were similar to that in Chinese adults. This provided reference data for future in-depth development and utilization of fragrance pig.展开更多
旨在探究 TAS1 R3基因通过mTOR对从江香猪自噬相关基因表达的影响。本试验选择30日龄健康雄性从江香猪3头,采集睾丸组织并培养出睾丸间质细胞。将构建成功的干扰载体和空载体转染至细胞中,利用qRT-PCR检测其对 TAS 1R3基因表达影响,运用...旨在探究 TAS1 R3基因通过mTOR对从江香猪自噬相关基因表达的影响。本试验选择30日龄健康雄性从江香猪3头,采集睾丸组织并培养出睾丸间质细胞。将构建成功的干扰载体和空载体转染至细胞中,利用qRT-PCR检测其对 TAS 1R3基因表达影响,运用qRT-PCR和Western blot检测 TAS1 R3基因干扰后对味觉受体第一家族一号受体(TAS1R1)、细胞外信号调节激酶1(ERK1 ) 、细胞外信号调节激酶2(ERK2)、雷帕霉素靶蛋白(mTOR)、 BECN1 和微管相关蛋白1轻链3β(MAP1LC3B)表达的影响。结果表明,当 TAS 1R3基因被干扰后,相较于shRNA-NC对照组, TAS 1R1、 ERK 1、 ERK 2、 mTOR 基因mRNA表达量均极显著降低( P <0.01),自噬因子 BECN1 和 MAP 1LC3B表达量极显著升高( P <0.01)。Western blot结果表明当 TAS 1R3基因被干扰后,与 shRNA-NC 对照组相比,蛋白T1R3、T1R1、p-mTOR、p-S6K1、p-ERK12均极显著降低( P <0.01),而自噬蛋白 Beclin 1 表达量极显著升高( P <0.01)。本试验通过将 TAS 1R3基因干扰载体转染至睾丸间质细胞,发现当 TAS 1R3基因被干扰后可通过影响 mTOR 基因的表达,调控其下游自噬因子的表达,提示 TAS 1R3基因与睾丸间质细胞的自噬密切相关,这为进一步研究 TAS 1R3基因通过调节自噬影响香猪生殖活动提供了借鉴。展开更多
文摘[Objective] The paper was to determine whether the germplasm resources of Congjiang fragrance pig had changed over the past 30 years. [Method]The growth development and reproductive performance of 100 pigs from six towns in central fragrance pig producing area and Congjiang fragrance pig stock seed farm were measured,and further compared with the data in Guizhou Livestock and Poultry Breeds 1986. [Result]The growth and development status was basically consistent,and the average litter size was at a downward trend: the first litter decreased by 26. 2%; the second litter decreased by 7. 0%; the third litter decreased by8. 55%. [Conclusion] The study laid the foundation for vigorous promotion of Congjiang fragrance pig in industrial development process,acceleration of breeding pace,gradual updating of farming breeds by farmers and improvement of production efficiency of fragrance pig.
文摘Fragrance pig is a famous miniature local breed in China,which is similar to human on the aspects of physical structure,anatomy,nutrition,metabolism and blood biochemical indicators. The internal organs of Congjiang fragrance pig and Huanjiang fragrance pig with different month ages were weighed. The results showed that the proportion of stomach in body weight in Congjiang fragrance pig was higher than that in Min pig,Harbin white and Landrace,and the proportion of large intestine in body weight in Congjiang fragrance pig was also higher than that in ordinary pigs; the weights of heart,liver and kidney in 8- 10 months old Congjiang fragrance pig were similar to that in Chinese adults. This provided reference data for future in-depth development and utilization of fragrance pig.
文摘旨在探究 TAS1 R3基因通过mTOR对从江香猪自噬相关基因表达的影响。本试验选择30日龄健康雄性从江香猪3头,采集睾丸组织并培养出睾丸间质细胞。将构建成功的干扰载体和空载体转染至细胞中,利用qRT-PCR检测其对 TAS 1R3基因表达影响,运用qRT-PCR和Western blot检测 TAS1 R3基因干扰后对味觉受体第一家族一号受体(TAS1R1)、细胞外信号调节激酶1(ERK1 ) 、细胞外信号调节激酶2(ERK2)、雷帕霉素靶蛋白(mTOR)、 BECN1 和微管相关蛋白1轻链3β(MAP1LC3B)表达的影响。结果表明,当 TAS 1R3基因被干扰后,相较于shRNA-NC对照组, TAS 1R1、 ERK 1、 ERK 2、 mTOR 基因mRNA表达量均极显著降低( P <0.01),自噬因子 BECN1 和 MAP 1LC3B表达量极显著升高( P <0.01)。Western blot结果表明当 TAS 1R3基因被干扰后,与 shRNA-NC 对照组相比,蛋白T1R3、T1R1、p-mTOR、p-S6K1、p-ERK12均极显著降低( P <0.01),而自噬蛋白 Beclin 1 表达量极显著升高( P <0.01)。本试验通过将 TAS 1R3基因干扰载体转染至睾丸间质细胞,发现当 TAS 1R3基因被干扰后可通过影响 mTOR 基因的表达,调控其下游自噬因子的表达,提示 TAS 1R3基因与睾丸间质细胞的自噬密切相关,这为进一步研究 TAS 1R3基因通过调节自噬影响香猪生殖活动提供了借鉴。
