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Characterization and Comparison of Ochratoxin A-Ovalbumin(OTA-OVA) Conjugation by Three Methods 被引量:1
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作者 WANG Xi-chun BAO Ming +6 位作者 WU Jin-jie LUO Ying MA Liang-you WANG Ying ZHANG Ai-hua HE Cheng-hua ZHANG Hai-bin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第5期1130-1136,共7页
In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten (ochratoxin A, OTA) was coupled to ovalbumin (OVA... In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten (ochratoxin A, OTA) was coupled to ovalbumin (OVA) by an active ester reaction. To develop a technique for detecting the conjugation, the hapten-protein conjugate (OTA-OVA) was characterized thoroughly by immunoarray technology, ultraviolet (UV) spectroscopy and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), respectively. The molecular weight of OTA-OVA was 50 350.141 Da, and the molecular weight of OVA was 44 887.506 Da, which were determined by MALDI-TOF-MS, respectively. In OTA-OVA, the molecular coupling ratio was 13:1 by MALDI-TOF-MS while the molecular coupling ratio was 10:1 by UV. In this experiment, UV and MALDI-TOF-MS were selected as the efficient methods to evaluate the cross-linking effect and calculate the molecular coupling ratio. 展开更多
关键词 ochratoxin A conjugated antigen cartier protein coupling ratio
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