Insights into constructing a stable and efficient microbial consortium system

The concept of labor division and multi-module cooperation of microbial consortia offers it promising potentials in various areas,such as the utilization of complex substrates,synthesis of natural compounds with long metabolic pathways and remediation of environmental pollutants within a hostile environment.Consequently,synthetic microbial consortia represent a new frontier for synthetic biology because they can solve more complex problems than monocultures.However,current research on microbial consortia often involves the simple mixing of multiphase systems,where strains are co-cultured sequentially or individually cultured and then mixed-cultured.The instability and low efficiency of microbial consortia systems hindered their practical application.To construct a stable and efficient microbial consortium,it is essential to consider the different growth and metabolic characteristics of strains,the competition for various nutrients as well as the complex carbon,energy and signaling dynamics within the system.In this review,we provide a progressive strategy for constructing a stable and efficient microbial consortium system across three stages:compromised stage(work together),microenvironment-oriented stage(work better),and metabolite delivery-enhanced stage(work best).The detailed methods and points for attention of each stage are summarized,with a highlight on the technical bottleneck and application limitations.Through the integration of interdisciplinary strategies,such as materials science and mathematical models,the goal of building a stable and efficient microbial consortium is constantly advanced.

Constructing Electron Microscope Labs: Challenges and Solutions

The construction of advanced laboratories for precision instruments, such as electron microscopes, involves unique challenges that are influenced by the specific environmental conditions required for optimal functionality. These include mitigating interference from magnetic fields and vibrations, which are critical for maintaining the precision and accuracy of the instruments used. This study aims to offer enhanced project management strategies and detailed construction solutions that address the environmental and technical needs specific to electron microscopy labs, thereby facilitating effective lab operations and extending the lifecycle of high-end precision instruments. Case studies of existing laboratory constructions, onsite investigations, and comprehensive reviews of the technical and environmental requirements provide the basis for a best practice for constructing sophisticated electron microscopy labs. The approach integrates both pre-construction planning and post-construction adjustments to create optimal operational environments. The findings suggest that successful lab constructions are those that incorporate thorough onsite assessments, strategic location choices, and the use of advanced construction materials and techniques specifically designed to counteract environmental challenges like magnetic and vibration interferences. Actionable guidelines for both planning and executing the construction of electron microscope labs highlighted in this tutorial are intended as an important resource to troubleshoot or upgrade existing lab facilities and to consult in preparation of future lab construction projects.

Rapid Constructing a Genetic Linkage Map by AFLP Technique and Mapping a New Gene tms5

In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was Egged and mapped onto chromosome 2 during the development of the linkage map.

Analysis on the Necessity and Feasibility of Implementing the Strategy of "Protecting Farmland in Flatland Areas and Constructing Mountainous Cities" in Yunnan Province

Yunnan is one of the most typical mountalnous provinces in western Chi-na. For the geographical features of mountalnous areas accounting for most parts and fIatIand areas accounting for smal parts, the contradiction between farmIand protection and construction guarantee is very prominent. In the first half of 2011, Yunnan Province estabIished creativeIy the strategy of ＆quot;protecting farmIand in fIat-Iand areas and constructing mountalnous cities＆quot; （hereinafter referred to as the strat-egy of ＆quot;constructing mountalnous cities＆quot;）, which was almed at protecting effectiveIy the Iimited and high quality arabIe Iand resources in fIatIand area and providing new construction area for new type of urbanization and industrialization, so as to achieve win-win situation between arabIe Iand protection and urbanization deveIopment. Im-pIementing this strategy in Yunnan Province is feasibIe, malnIy in the Iarge deveIop-ment potentials in the gentIe gradient between 8° to 25°. The gentIe sIope area be-tween 8° to 25° occupies 51% of the total Iand area in Yunnan Province, which is almost 140 times of the area of urban construction Iand in Yunnan in 2009. The surrounding areas of fIatIand or maln town in most counties （cities, regions） dis-tribute with different sizes of Iow hiI s and gentIe sIopes, thus mountalnous construc-tion Iand reserves are abundant, which benefits for impeI ing the strategy of ＆quot;con-structing mountalnous cities＆quot; in the Iight of Iocal conditions.

Study on Urban Construction Land Suitability Evaluation in Southwestern Mountainous Areas of Yunnan Province Based on the Strategy of "Protecting Farmland in Flatland Areas and Constructing Mountainous Cities" : A Case in Lianghe County

According to the particularity of towns＇ construction in the southwestern mountainous areas of Yunnan Province, this study has built constructive land suit- ability evaluation index system which is different to flat area. Then this study deter- mined single evaluation factors index and comprehensive suitability index using ＂ex- treme conditions method＂ and ＂suitability index method＂, and analyzed and assessed the constructive land suitability on each evaluation unit polygons and its suitability level by using GIS software. Taking Lianghe County of Dehong Dai-Jingpo Au- tonomous Prefecture as an example to evaluate land suitability in Lianghe County＇s main towns around the gentle slope, the results show, in the evaluation area, the area of land suitable for construction and unsuitable for construction respectively ac- count 73.58% and 26.42%. And first-class, second and third-class of land suitable for construction should be composited at the ratio of about 11：49：40. The suitability evaluation index system and method developed in this paper is applicable for evalu- ating the construction land suitability in mountainous areas of southwest Yunnan Province, and will provide theoretical and technical support for land policy measures which strengthen the protection of farmland and promote the scientific development of urbanization in Yunnan Province and even similar mountainous areas of the whole China.

Recent advances of metal vacancies in energy and environmental catalysis:Synthesis,characterization,and roles

With the ongoing depletion of fossil fuels,energy and environmental issues have become increasingly critical,necessitating the search for effective solutions.Catalysis,being one of the hallmarks of modern industry,offers a promising avenue for researchers.However,the question of how to significantly enhance the performance of catalysts has gradually drawn the attention of scholars.Defect engineering,a commonly employed and effective approach to improve catalyst activity,has become a significant research focus in the catalysis field in recent years.Nonmetal vacancies have received extensive attention due to their simple form.Consequently,exploration of metal vacancies has remained stagnant for a considerable period,resulting in a scarcity of comprehensive reviews on this topic.Therefore,based on the latest research findings,this paper summarizes and consolidates the construction strategies for metal vacancies,characterization techniques,and their roles in typical energy and environmental catalytic reactions.Additionally,it outlines potential challenges in the future,aiming to provide valuable references for researchers interested in investigating metal vacancies.

Research on the Construction of Intellectual Property System and Optimization of Business Environment in China

With the rapid development of globalization and information technology,intellectual property has been one of the key drivers of economic growth,and the construction of intellectual property system has become an important criterion for measuring the quality of business environment.This article is intended to explore the current status of intellectual property system construction in China,the challenges,and its relationship with the business environment,to propose the corresponding countermeasures and suggestions.The study finds that the legal system of intellectual property in China is gradually improving,and judicial and administrative protection are continuously strengthened.However,the challenges still remain such as frequent infringements,rights hard to protect and insufficient international cooperation.These issues not only affect the legitimate rights and interests of innovation entities,but also for the market fairness and the level of the business environment.Therefore,this article proposes that strengthening the perfection of the intellectual property legal system,enhancing intellectual property services and support capabilities,strengthening international cooperation and exchanges,and accelerating the cultivation of composite talents.It aims to provide theoretical references for the construction of intellectual property system and the optimization of the business environment,promote the high-quality development of economy and enhance the global competitiveness of the country.

The Scale of Constructing Mountainous Cities in Yunnan Province based on “Benchmark” Farmlands

In 2011, Yunnan provincial committee and government proposed the strat- egy of constructing mountainous cities in order to protect farmlands and guarantee crop safety, which makes full use of hills and gentle-slopes and transforms land use pattern. However, social and economic development performs unevenly in Yun- nan, supplemented by varied geographical conditions, so that constructing mountain- ous cities should proceed as per local conditions. The research proposed the con- trol of constructing mountainous cities provided crop safety under the principle of farmland protection and measured the quantities of demanded construction lands ac- cording to science of population prediction and ekistics, based on which the control scales of constructing mountainous cities were determined by multiplying basic farm- land coefficient and the area of newly increased construction lands. The research is of significance for guiding the construction of mountainous cities and avoiding the occupation of construction lands on farmlands.

Risk Management Techniques in Construction Projects:Development and Assessment of an Innovative

Professional and trade skills are required for handling the construction related projects;Construction industries of the present day however lack useful information concerning different practices,patterns and trends involved in risk management.Considering this,the present study focuses on the aforementioned variables of risk management by quantitative analysis specifically in the domain of construction industry.This study has used IBM’s SPSS(Statistical Package for Social Sciences)version 25.0 to analyze the results.This study is an initiative to assess the impact of risk management in the construction sector of Jordan.It will assist the construction sector for exploring the limitations with respect to integrate effective risk management.A sense of competition will be developed through a comparison of risk factors of construction projects among the project stakeholders such as contractors should enhance their risk management practices.

Analysis of Claims in the Uganda Construction Industry

The increasing cost of infrastructure development risks stifling national investment plans.Many projects in Uganda have suffered because of the frequency and magnitude of claims made by parties involved especially the contractors.As a result,the construction industry is plagued with claims which quite often lead to adversarial relationships.The major grounds for claims were identified through studying the contract documents where claims had been made.Increase in scope;change orders;errors in contract documents;inclement weather;inflation;delay in possessing the site;and change in the tax policy were the most prevalent grounds for making claims.Problems identified in claiming process include delays in getting approval;information on claims not reliable;hostility from the parties involved;pricing of claims;delays in effecting payment when approved;and overzealous contract interpretation leading to misunderstanding and delays Using a survey on project managers for 64 contractors and their corresponding clients or their representatives,the major ways in which it can be reduced were identified as being thoroughness during the design phase;providing for inflation;proper planning of works;using prequalified contractors;and proper documentation of records.Clients have a big role to play in reducing claims.

Construction Monitoring and Analysis of Asymmetric Prestressed Concrete Bridge Crossing Multiple-Line Railways

Complex bridge structures designed and constructed by humans often necessitate extensive on-site execution,which carries inherent risks.Consequently,a variety of engineering practices are employed to monitor bridge construction.This paper presents a case study of a large-span prestressed concrete(PC)variable-section continuous girder bridge in China,proposing a feedback system for construction monitoring and establishing a finite element(FE)analysis model for the entire bridge.The alignment of the completed bridge adheres to the initial design expectations,with maximum displacement and pre-arch differences from the ideal state measuring 6.39 and 17.7 mm,respectively,which were less than the 20 mm limit required by the specification.Additionally,the stress monitoring showed that the maximum compressive stress was 10.44 MPa,which was 7.5%different from the finite element results,and better predicted the most unfavorable possible location.These results demonstrate that a scientifically rigorous construction monitoring and feedback system can ensure the safety of bridge construction and meet the expected construction standards.The findings presented in this paper provide valuable insights for bridge construction monitoring practices.

Predicting the Construction Quality of Projects by Using Hybrid Soft Computing Techniques

The construction phase of a project is a critical factor that significantly impacts its overall success.The construction environment is characterized by uncertainty and dynamism,involving nonlinear relationships among various factors that affect construction quality.This study utilized 987 construction inspection records from 1993 to 2022,obtained from the Taiwan residents Public Construction Management Information System(PCMIS),to determine the relationships between construction factors and quality.First,fuzzy logic was applied to calculate the weights of 499 defects,and 25 critical construction factors were selected based on these weight values.Next,a deep neural network was used to identify the relationship between the critical construction factors(input variables)and construction quality(output variable).Finally,the prediction model’s performance was evaluated to confirm the impact of these critical construction factors on project outcomes.This study employed an innovative hybrid soft computing technique,com-bining fuzzy logic and an artificial neural network,to effectively predict the relationship between critical construction factors and construction quality,achieving a model accuracy of 96.08%.Project managers can utilize the findings of this study to enhance project management practices and establish effective construction management strategies,thereby improving project construction quality.

Data-Driven Method for Predicting Remaining Useful Life of Bearings Based on Multi-Layer Perception Neural Network and Bidirectional Long Short-Term Memory Network

The remaining useful life prediction of rolling bearing is vital in safety and reliability guarantee.In engineering scenarios,only a small amount of bearing performance degradation data can be obtained through accelerated life testing.In the absence of lifetime data,the hidden long-term correlation between performance degradation data is challenging to mine effectively,which is the main factor that restricts the prediction precision and engineering application of the residual life prediction method.To address this problem,a novel method based on the multi-layer perception neural network and bidirectional long short-term memory network is proposed.Firstly,a nonlinear health indicator(HI)calculation method based on kernel principal component analysis(KPCA)and exponential weighted moving average(EWMA)is designed.Then,using the raw vibration data and HI,a multi-layer perceptron(MLP)neural network is trained to further calculate the HI of the online bearing in real time.Furthermore,The bidirectional long short-term memory model(BiLSTM)optimized by particle swarm optimization(PSO)is used to mine the time series features of HI and predict the remaining service life.Performance verification experiments and comparative experiments are carried out on the XJTU-SY bearing open dataset.The research results indicate that this method has an excellent ability to predict future HI and remaining life.

Problems of Rural Food Safety and Strategies of Constructing Supervision System

This paper expounds the practical necessity of constructing diversified rural food safety supervision system as follows: it is the necessary requirements of guaranteeing people's health and life safety; it is an important component of governmental function of social management and the logical extension of administrative responsibilities; it is the basis of maintaining order of rural society and constructing harmonious society. The main problems existing in the supervision of rural food safety are analyzed as follows: first, the legislative work of rural food safety lags behind to some extent; second, the supervision of governmental departments on rural food safety is insufficient; third, the industrial supervision mechanism of rural food security is not perfect; fourth, the role of rural social organizations in supervising food safety is limited; fifth, the farmers' awareness of food safety supervision is not strong. Based on these problems, the targeted strategies of constructing diversified rural food safety supervision system are put forward as follows: accelerate the legislation of rural food safety, and ensure that there are laws to go by; give play to the dominant role of government, and strengthen administrative supervision on rural food safety; perfect industrial convention of rural food safety, and improve industrial supervision mechanism; actively support the fostering of social organizations, and give play to the role of supervision of organizations; cultivate correct concept of rights and obligations of farmers, and form awareness of food safety supervision.

Fusing Fixed and Hint Landmarks on Crowd Paths for Automatically Constructing Wi-Fi Fingerprint Database

In typical Wi-Fi based indoor positioning systems employing fingerprint model,plentiful fingerprints need to be trained by trained experts or technician,which extends labor costs and restricts their promotion.In this paper,a novel approach based on crowd paths to solve this problem is presented,which collects and constructs automatically fingerprints database for anonymous buildings through common crowd customers.However,the accuracy degradation problem may be introduced as crowd customers are not professional trained and equipped.Therefore,we define two concepts:fixed landmark and hint landmark,to rectify the fingerprint database in the practical system,in which common corridor crossing points serve as fixed landmark and cross point among different crowd paths serve as hint landmark.Machinelearning techniques are utilized for short range approximation around fixed landmarks and fuzzy logic decision technology is applied for searching hint landmarks in crowd traces space.Besides,the particle filter algorithm is also introduced to smooth the sample points in crowd paths.We implemented the approach on off-the-shelf smartphones and evaluate the performance.Experimental results indicate that the approach can availably construct WiFi fingerprint database without reduce the localization accuracy.

Research on prevention and cure against heat-harm in constructing mines

With the mine exploitation depth getting deeper and deeper, the mine heat-harm turns into another natural disaster after the five disasters, which are the roof, the gas, the fire, the dust and the flood disaster. Compared with other researches on prevention and cure against the heat-harm, the research on prevention and cure against the heat-harm in constructing mine are less. Referring to the experience of prevention and cure against the heat-harm home and ＇abroad, analyzing the characteristic of cooling in the constructing mine, and based on the ground former freezing equipments and the modified mine temporary cooling equipments, the paper proposed four technical projects. By comparing with the four technical projects, the second project is preponderant both in technology and economy and can solve the heat-harm of the constructing mine, which is proved by the forecast and calculation. The project can be widely applied in de-temperature tasks of high temperature and heat-harm mines during the mine constructing period and in the exploiting preparing phase.

Constructing Non-binary Asymmetric Quantum Codes via Graphs

The theory of quantum error correcting codes is a primary tool for fighting decoherence and other quantum noise in quantum communication and quantum computation. Recently, the theory of quantum error correcting codes has developed rapidly and been extended to protect quantum information over asymmetric quantum channels, in which phase-shift and qubit-flip errors occur with different probabilities. In this paper, we generalize the construction of symmetric quantum codes via graphs (or matrices) to the asymmetric case, converting the construction of asymmetric quantum codes to finding matrices with some special properties. We also propose some asymmetric quantum Maximal Distance Separable (MDS) codes as examples constructed in this way.

Rational Thoughts on Reconstructing Rural Resident's Living Style

The construction of rural residents＇ living style is the fundamental significance of building the new socialist countryside. With the deep development of the urban and rural, rural residents＇ living style has changed profoundly on the labour, consumption, entertainment, marriage and family, etc., and the differences of living style between the urban and rural are becoming smaller and smaller. Therefore, this paper suggested to promote the rural residents to build the scientific, cultural and healthy modern life style, and put forward the rational development thoughts.

A Study of the European Union’s Path for Constructing Digital Governance Rules and the Logical Implications of the Path

The European Union(EU)seeks to build a Europe fit for the digital age.For this purpose,the EU has accelerated the process of catching up with digital technology and issued a number of legal and regulatory documents to establish a digital governance rule regime with EU characteristics.This paper analyzes the EU’s path towards the construction of digital governance rules.This path is composed of three aspects,i.e.,strict digital supervision,differentiation of the free market,and multi-stakeholder governance.This three-step path has intrinsic logical implications.That is,strict digital supervision is applied to defend the EU’s unified market and values;differentiation of the free market takes the principle of adequate protection as the core to make the EU an exporter of rules;multi-stakeholder governance brings multiple stakeholders together in the governance to deal with the ethical anomie that arises during the use of big data.By setting up a series of digital governance rules,the EU seeks to achieve rule dominance to gain regulatory power for global digital governance.However,due to multiple constraints,there is a long way to go,and there are obstacles in the way.

Constructing Teaching Model for Training English Guides of Stone Inscription Relics

A teaching model based on constructivism is proposed in this paper. The model contains five teaching steps, e.g. interpretation teaching, questioning- dialogue, knowledge and skills teaching, discussion- collaboration and field training. Practice proves that it can effectively improve the training efficiency of the training of English guides of stone inscription relics and enhance their interpretation quality and English skills.