Effects of dietary fiber content and different fiber-rich ingredients on endogenous loss of fat and fatty acids in growing pigs

Background: Determination of the endogenous loss of fat(ELF) is used to adjust for the estimation of true total tract digestibility(TTTD) of fat in diets and ingredients.Any factor which affected ELF may further affect the digestibility of fat,including sources and concentrations of fat and fiber in the diet.There are some reports of determining the ELF using regression methods based on different levels of fat intake,while reports on effects of dietary fiber content and different fiber-rich ingredients in pig diets on ELF are very limited.Therefore,the objective of this study was to determine the effects of dietary fiber content and different fiber-rich ingredients on endogenous losses of fat and fatty acids at the end of ileum and throughout the entire intestinal tract in growing pigs.Methods: In Exp.1,the effect of fiber content on endogenous loss of fat was determined using six growing pigs(Duroc × Landrace × Yorkshire;27.6 ± 2.4 kg),fitted with a T-cannula at the end of ileum.The experimental design was a 6 × 6 complete Latin square design with six periods of feeding and six diets.The six experimental fat-free diets were formulated to include graded levels of neutral detergent fiber(NDF)(0,40,80,120,160 and 200 g/kg) and soybean hull(SH) was the only fiber source,providing 0,75,150,225,300 and 375 g/kg,respectively.Chromic oxide was included at4 g/kg in all diets as an indigestible marker.In Exp.2,six crossbred growing barrows(27.6 ± 1.6 kg) were used and the experimental design was the same as for Exp.1.The six fat-free diets were formulated to include six common fiber-rich ingredients and the concentration of NDF was 100 g/kg.The six fiber-rich ingredients were defatted rice bran(DRB),sugar beet pulp(SBP),rice hull(RH),corn germ meal(CGM),SH and wheat bran(WB) and they were fed at represented250,270,145,250,170 and 280 g/kg in the diet,respectively.Results: In Exp.1,the endogenous loss of fatty acids profile did not change as dietary NDF increased in growing pigs.The endogenous losses of fat,C16:0,C18:0,C18:1,C18:2,total unsaturated fatty acids(UFA) and total saturated fatty acids(SFA) in growing pigs at the end of ileum and throughout the entire intestinal tract increased linearly as NDF content of diets increased.The endogenous losses of fat,as well as C16:0 and C18:0 throughout the entire intestinal tract also increased quadratically as NDF content of diets increased.The ELF increased from 0.71 to 3.14 g/kg of dry matter intake(DMI) and 0.56 to 8.21 g/kg DMI at the end of ileum and throughout the entire intestinal tract in growing pigs,respectively.The ELF occurred in the hindgut except for the growing pigs fed 0 and 4% NDF in their diets.The endogenous losses of C16:0 and UFA occurred primarily in the upper regions of the gut and the greatest endogenous losses of C18:0 occurred in the hindgut.The endogenous losses of fat,individual SFA and total SFA throughout the entire intestinal tract were much greater than that at the end of ileum.However,the endogenous losses of individual UFA and total UFA were less throughout the intestinal tract than at the end of ileum.In Exp.2,the endogenous losses of fat at the end of ileum were greater in growing pigs fed CGM or WB diets.The endogenous loss of fatty acids profile changed to a slight degree at the end of ileum that the endogenous loss of UFA(particularly C18:1 and C18:2) in growing pigs fed CGM or WB diets were greater(P < 0.01) than that for the other four diets.The greatest(P < 0.01) endogenous loss of SFA(particularly C18:0) was in growing pigs fed the RH diet.The endogenous losses of fat,C16:0,C18:0 and SFA over the entire intestinal tract were much greater in growing pigs fed CGM or WB diets,whereas the lowest values were in growing pigs fed DRB diet.The ELF at the end of ileum in growing pigs fed CGM or WB diets were 3.50 or 4.17 g/kg DMI,respectively,and the ELF over the entire intestinal tract was 7.23 or 7.38 g/kg DMI.The contribution in percentage of ELF in the upper gut was greater than that in the hindgut of growing pigs fed DRB and RH diets,while the ELF in the upper gut and hindgut were equal in growing pigs fed SBP,CGM and WB diets.On the whole,the endogenous losses of C18:1 and C18:2 throughout the entire intestinal tract in growing pigs fed the six fiber-rich ingredients diets were less than losses at the end of ileum,whereas the endogenous loss of fat,C16:0,C18:0 and SFA were greater throughout the intestinal tract than at the end of ileum.Conclusion: The profile of loss in endogenous fatty acids did not change as dietary NDF increased in growing pigs and the endogenous losses of fatty acids(C16:0,C18:0,C18:1 and C18:2) fat,UFA and SFA increased linearly as NDF content increased in the diets of pigs.The endogenous losses of fat or fatty acids at the end of ileum were greater in growing pigs fed RH,CGM or WB diets.The endogenous losses of fat,fatty acids(C16:0 and C18:0) and SFA were greater over the entire intestinal tract in pigs fed CGM or WB diet,while these values were the lowest in growing pigs fed the DRB diet.The contribution in percentage losses of fat in the upper gut were greater than in the hindgut of growing pigs fed DRB and RH diets,while the contribution of losses of fat in the upper gut and hindgut were equal in growing pigs fed SBP,CGM and WB diets.In addition,the endogenous loss of individual or total UFA was less over the entire intestinal tract of growing pigs fed fiber diets than that at the end of ileum,and the greatest endogenous losses of fat,individual or total SFA were over the entire intestinal tract.Therefore,differences in fiber content and the nature of fiber-rich ingredients in diets of pigs have different effects to the endogenous losses of fat or fatty acids.Considering the requirement of fat or fatty acids of pigs,careful attention must be paid that the endogenous losses of fat and fatty acids when fiber ingredients are used in diets of pigs.

黄芪注射液中初生成分的鉴定及氨基酸类成分的含量测定

目的 鉴定黄芪注射液中的初生成分,并以氨基酸类成分含量为指标,评价不同来源黄芪注射液的质量差异。方法 采用液相二级质谱(LC-MS/MS)法,以0.1%甲酸乙腈-0.1%甲酸水为流动相,采用梯度洗脱检测黄芪注射液中初生成分。采用LC-MS/MS法,以10%甲醇水(含0.1%甲酸)和50%甲醇水(含0.1%甲酸)为流动相,采用梯度洗脱测定黄芪注射液中各氨基酸的含量,并采用主成分分析评价不同厂家黄芪注射液中氨基酸的含量差别。结果 不同厂家黄芪注射液中均检测到255个初生成分,主要为氨基酸类、有机酸类、糖类和含N类化合物。4个厂家黄芪注射液中氨基酸含量得分分别为-0.23,-1.77,-0.35,2.36。结论 不同厂家黄芪注射液中初生成分的种类一样,但含量不同;不同厂家黄芪注射液中氨基酸的含量差异较大。

高效液相色谱法测定氟苯尼考原料药

本研究建立了氟苯尼考药的液相色谱测定方法。通过研究色谱柱分离、定性波长、流动相选择等条件确定检测方法。并通过检出限、定量限、回收率和重复性实验评价方法。结果表明,选择TC-C18柱作为分离柱,最佳波长为223 nm,最佳流动相为25%乙腈水溶液,氟苯尼考线性达到0.99975,检出限为0.05μg/mL,定量限为0.15μg/mL,添加量为0.5~3μg时回收率达到88.7%~100%,重复性RSD达到0.64%。此方法高效准确,能为氟苯尼考药主含量测定提供参考,也能为其他兽药氟苯尼考的测定提供思路。

舒心解郁颗粒制备工艺及关键成分含量测定研究

目的:对舒心解郁颗粒的制备工艺进行研究,采用高效液相色谱法同时测定舒心解郁颗粒中7种成分的方法,以期为舒心解郁颗粒质量评价与临床应用提供科学依据。方法:采用正交试验优选处方中药材的煎煮工艺,以浸出率为指标,优选提取工艺。对辅料、药辅比、润湿剂及干燥温度进行考察,优选成型工艺。同时采用HPLC法测定制剂中洋川芎内酯Ⅰ、阿魏酸、斯皮诺素、芍药苷、3,5-O-二咖啡酰基奎宁酸、绿原酸、没食子酸的含量。结果:确定最优提取工艺:加入12倍量水,每次40 min,提取3次。成型工艺:用糊精作辅料,药辅比1∶1.8,80%乙醇作为润湿剂,65℃干燥。采用优选的制备工艺生产6批舒心解郁颗粒,建立成品中洋川芎内酯Ⅰ、阿魏酸、斯皮诺素、芍药苷、3,5-O-二咖啡酰基奎宁酸、绿原酸、没食子酸含量测定方法,测定舒心解郁颗粒成分含量。结论:该研究建立的舒心解郁颗粒制备工艺稳定合理、成型率高,为大批量生产舒心解郁颗粒提供了切实可行的方法,且本研究建立的含量测定方法,具有操作简便,重复性和稳定性好等特点,可为舒心解郁颗粒及相似的中药复方制剂的质量控制研究提供借鉴。

食物中唾液酸含量分布研究进展

目的:唾液酸广泛存在于多种生物组织中,有50多种存在形式,其中,N-乙酰神经氨酸(Neu5Ac)和N-羟乙酰神经氨酸(Neu5Gc)是最主要的2种存在形式。唾液酸具有抗炎、抗病毒和抗肿瘤等多种生物活性,并能有效促进婴儿的认知发育、增强学习和记忆能力。目前中国食物成分表中无唾液酸含量数据,本研究旨在总结和分析各类食物中唾液酸分布及含量,并推测每日膳食中可能的唾液酸摄入量。方法:在PubMed、Embase、Web of Science、中国知网、万方数据库、维普检索自建库至2022年6月国内外公开发表的相关研究文献。纳入测定食物中唾液酸分布与含量的定性与定量研究,包括一手研究数据、综述、系统综述等非一手研究数据,语言限定为中英文、意大利语;排除与主题无关、重复、数据不完整、无法获得全文的文章。结果:共检索出文献11187篇,本文共纳入35篇文献作为本综述的主要证据,其中,奶及奶制品、动物性食物、蔬菜类、水果类、谷薯类、其他食物文献各15、9、3、2、4、4篇。研究显示,燕窝中唾液酸含量最高,可高达116.2 mg/g。其次是动物性食物、奶及奶制品。动物性食物中唾液酸含量在0~4574.298μg/g、奶及奶制品中唾液酸含量在28~1596 mg/L。谷类、蔬菜类、水果类食物中含有微量或不含唾液酸,油、薯类中不含唾液酸。总结:动物性食物、奶及奶制品含有唾液酸,大豆及坚果类、蔬菜类、水果类、谷类中含有微量或不含唾液酸。

不同产地党参体外抗氧化活性研究

目的:测定58批党参样品中多糖、黄酮、皂苷的含量及体外抗氧化活性,综合评价党参产地、活性成分含量及抗氧化活性之间的关系。方法:建立紫外-可见分光光度法、1,1-二苯基-2-三硝基苯肼及2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐法测定党参样品活性成分含量,采用聚类分析、双变量相关性分析、偏最小二乘法-回归分析及灰色关联度分析党参产地、活性成分含量及抗氧化活性之间的关系。结果:58批党参样品中总黄酮、总多糖、总皂苷的质量浓度分别为0.418~1.396、4.954~15.822、12.111~52.052 mg·mL^(-1)。聚类分析、偏最小二乘法-回归分析显示58批党参可以较好地区分为2类,第1类为重庆、湖北、四川、内蒙古及山西产党参,第2类为甘肃渭源县、临洮县、漳县及陇南文县、宕昌县产党参,且抗氧化活性受产地的影响。双变量相关性分析结果表明黄酮类物质可能是影响抗氧化活性的一个显著因素。采用灰色关联度分析得出黄酮的含量与党参抗氧化活性密切度较高,与双变量相关性分析的结果相一致。结论:58批党参均具有一定的抗氧化活性,且抗氧化活性受产地和活性成分含量的影响。

超高温瞬时灭菌对米酒品质的影响

目的:使经过超高温瞬时灭菌的米酒既符合商业上无菌要求也满足消费者对米酒质量的要求。方法:对朝鲜族米酒进行超高温瞬时灭菌,在贮藏期每周测定其理化指标、感官指标以及微生物指标以确定该灭菌法的最佳灭菌条件,并分析此灭菌方法处理的朝鲜族米酒在贮存20周后的品质和功能性成分的变化。结果:超高温瞬时灭菌的最佳参数为125℃灭菌5 s。贮存20周后,米酒中总氨基酸含量为0.70 mg/100 mL,有机酸含量为321.68 mg/100 mL。结论:超高温瞬时灭菌方法对氨基酸含量有一定负面影响,但能在保证米酒风味的同时高效灭菌。

高效液相色谱法测定化妆品中12种美白成分

建立了同时测定化妆品中的12种美白成分的高效液相色谱分析方法。采用Aglient HC-C_(18)色谱柱(4.6mm×250 mm×5μm)分离,以甲醇-0.02 mol·L^(-1)磷酸二氢钾溶液为流动相梯度洗脱,二极管阵列检测器多波长检测分析,检测波长为230、250、280 nm。结果表明,12种美白成分在1~50 mg·L^(-1)范围内呈良好的线性关系,相关系数均为0.999以上,检出限为1.0~7.0 mg·kg^(-1),定量限为3.5~22.0 mg·kg^(-1),添加量为20~200 mg·kg^(-1)时平均回收率为88.5%~105.7%,相对标准偏差为0.47%~3.60%。该方法前处理步骤简单,分析时间短,经方法学验证,可同时测定化妆品12种美白成分的含量。

白茶品质成分分析及在化妆品中的作用初探

以6种不同季节、等级的白茶为研究对象,采用水提醇沉法、醇浸提法进行提取,测定并比较生化成分表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)、总多糖、总黄酮、生命元素、氨基酸、维生素的含量,发现夏季的白牡丹多糖、EGCG含量最高,总黄酮、生命元素、维生素含量第二,整体成分含量优势明显,为白茶在化妆品中的充分利用提供数据支持。

成熟度及处理方式对胡椒活性成分及其含量的影响

以海南胡椒为材料,研究了胡椒成熟过程中活性成分胡椒碱、胡椒精油得率的动态性变化,以及采后不同处理方式对胡椒碱和胡椒精油得率及胡椒精油成分的影响。结果表明:不同成熟度的胡椒中胡椒碱与胡椒精油得率有显著差异,当成熟度为7~8时,胡椒碱和胡椒精油得率均最高。不同处理方式对胡椒碱和胡椒精油得率及胡椒精油成分均有显著影响,若以胡椒精油为目标物,以冻干处理为佳;若以胡椒碱为目标物,加工的白胡椒含量最高。不同处理方式后胡椒精油成分虽有差异,但整体组分以萜类物质为主。

余甘子鲜果不同部位中7种有效成分的含量比较

目的 建立同时测定余甘子鲜果不同部位(果肉、果皮、果核、果汁、果渣)中没食子酸、表没食子儿茶素、咖啡酸、没食子儿茶素没食子酸酯、诃黎勒酸、鞣花酸和槲皮素含量的方法,考察余甘子鲜果不同部位主要活性成分的分布情况,为新鲜余甘子的研究及开发利用提供参考。方法 采用Agilent ZORBAX SB-C_(18)(4.6 mm×250 mm,5μm)色谱柱;流动相为甲醇(B)-0.1%磷酸溶液(D);流速为1 mL·min^(-1);检测波长270 nm;柱温25℃;进样量10μL。并以AIA格式导出余甘子鲜果不同部位的色谱图,使用中药色谱指纹图谱相似度评价系统计算相似度。结果 7种活性成分在不同余甘子鲜果部位含量有明显差异,其中槲皮素为果皮特有,表没食子儿茶素未在果核中检测出;果核中整体化合物含量均低于其他部位,但鞣花酸含量显著较高;果渣中咖啡酸、没食子儿茶素没食子酸酯与诃黎勒酸含量可达其他部位的2倍,而没食子酸、表没食子儿茶素、鞣花酸的含量与果汁相差较小;果汁中表没食子儿茶素含量明显高于其他部位,与果肉比较,其他化合物含量差异较小;余甘子鲜果果肉、果皮、果核、果汁、果渣的相似度分别为0.993、0.997、0.728、0.990、0.988。结论 该方法简便、快速、可靠,可同时测定余甘子鲜果不同部位中7种成分的含量;余甘子鲜果不同部位有效成分含量存在明显差异。

高温瞬时灭菌对开心散有效成分含量及微生物学指标的影响

目的:比较不同高温瞬时灭菌条件下开心散的灭菌效果,结合灭菌后样品中有效成分含量的变化,确定最佳灭菌条件。方法:采用高温瞬时灭菌法对2~8目的远志、人参、茯苓和石菖蒲4味中药的粗颗粒进行灭菌,分别考察160、165、170℃3个温度水平,灭菌时间分别为5、7、9 s。在洁净区对灭菌粗颗粒分别进行粉碎,过六号筛后均匀混合,制得开心散。参照《中华人民共和国药典》2020年版进行微生物限度检测,并采用高效液相色谱法对开心散灭菌前后的样品进行含量检测。结果:9个灭菌条件下开心散样品的需氧菌总数均小于1×104CFU·g^(–1)、霉菌和酵母菌总数均小于1×102CFU·g^(–1)。同时,控制菌项下的大肠埃希菌、沙门菌、耐胆盐革兰阴性菌均未检出,所有开心散灭菌样品均符合口服散剂的微生物限度要求。开心散中8个有效成分(远志[口山]酮Ⅲ、3,6′-二芥子酰基蔗糖、细叶远志皂苷、人参皂苷Rg1、人参皂苷Rb1、茯苓酸、β-细辛醚和α-细辛醚)的含量受不同灭菌温度和时间影响。结论:结合需氧菌、霉菌和酵母菌、控制菌结果和有效成分含量变化情况,最终确定灭菌温度160℃、灭菌时间5 s为开心散最佳高温瞬时灭菌条件。

铁水成分对成品氮含量影响的分析

从铁水各成分元素着手,深入探讨对成品氮含量的控制。通过对现场数据进行统计分析,得出控制成品氮含量的有效控制方向,并从脱氧、造渣等方面提出了改进措施。

平凉刺五加叶生药学研究及其有效成分含量测定

采用性状鉴别和显微鉴别法对平凉刺五加植株进行定性分析,并采用高效液相色谱法测定平凉刺五加叶中有效成分的含量。性状鉴别观察到平凉刺五加叶的形态多为椭圆状倒卵形或长圆形,基部阔楔形,边缘重锯齿,具有特殊香气。显微鉴别显示,平凉刺五加叶粉末中多含有不定式气孔、螺纹导管、草酸钙簇晶、纤维等。刺五加苷B、刺五加苷E的回归方程具有良好的线性关系,精密度、稳定性、重复性试验的RSD均小于5.00%。3种提取方法中微波超声协同萃取法有效成分含量最高,刺五加苷B、刺五加苷E含量分别为0.085%、0.358%;微波萃取法次之,含量分别为0.075%、0.328%;超声辅助提取法含量最低,分别为0.054%、0.222%。

高效液相色谱法同时测定六堡茶中6种风味物质的含量

建立高效液相色谱法同时测定六堡茶中没食子酸、表没食子儿茶素没食子酸酯、表儿茶素没食子酸酯、儿茶素没食子酸酯、表儿茶素、没食子儿茶素6种风味物质的含量。在液料比为20∶1(mL∶g)条件下,茶叶粉末置于体积分数为60%乙醇溶液中,于50℃、180W功率超声提取20min后过滤,滤液经0.22μm滤膜再次过滤后进行检测,以Thermo Scientific Acclaim 120 C_(18)色谱柱为固定相,不同体积比的0.05%(体积分数)H3PO4溶液和乙腈混合液为流动相进行梯度洗脱,并在270nm波长处采用外标法定量检测。结果表明,在20min内可实现6种物质的有效分离,且在一定浓度范围内与相应的峰面积呈良好的线性关系(r>0.998)。该方法对不同物质的加标回收率为90.9%~98.8%,结果准确度较好,可为相关产品的质量评价提供参考。

南岳云雾茶的品质评价及其方法学研究

目的:建立茶叶中茶氨酸、5种儿茶素类(+C、EC、EGC、EGCG、ECG)、总黄酮和咖啡因含量测定的HPLC法,并对南岳云雾茶的品质进行评价。方法:茶叶中有效成分的提取参照GB/T 8303-2018,分析采用安捷伦Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm),流动相为2%乙酸水(A)-2%乙酸乙腈(B),流速0.8 mL/min^(-1),柱温25℃,检测波长为203 nm、278 nm、360 nm。结果:方法学结果显示+C、EC、EGC、EGCG、ECG、咖啡因、L-茶氨酸及芦丁分别在50.00μg∙mL^(-1)~150.00μg∙mL^(-1),50.00μg∙mL^(-1)~150.00μg∙mL^(-1),50.00μg∙mL^(-1)~150.00μg∙mL^(-1),100.00μg∙mL^(-1)~250.00μg∙mL^(-1),100.00μg∙mL^(-1)~300.00μg∙mL^(-1),10.00μg∙mL^(-1)~100.00μg∙mL^(-1),100.00μg∙mL^(-1)~400.00μg∙mL^(-1)及10.00μg∙mL^(-1)~100.00μg∙mL^(-1)浓度范围内线性关系良好,加样回收率为92.49%-105.56%。在上述条件下,测得南岳云雾茶中茶氨酸、儿茶素类、总黄酮和咖啡因的含量分别为67.32 mg/g、260.42mg/g、3.712 mg/g和34.05 mg/g。结论:南岳云雾茶中茶氨酸、儿茶素类及总黄酮的含量相对较高。该方法简便、准确、快速、重现性好,可为茶叶的质量控制提供科学的依据。

峨眉山天麻中主要活性成分天麻素的含量测定

通过对峨眉山天麻中主要活性成分天麻素的含量测定研究,为进一步深入研究天麻资源学与可持续利用提供科学依据。使用色谱柱为Hypersic BDS C18(200 mm×φ4.0 mm,5μm),柱温为30℃,检测波长为220 nm,流动相为体积比3∶97的乙腈-0.05%H_(3)PO_(4)溶液,流速为0.8 mL/min的反相高效液相色谱法(RP-HPLC)进行含量测定。测定出峨眉山天麻两组样品中主要活性成分天麻素的平均含量分别达到0.5323%和0.4232%,均大于0.0200%,而且理论塔板数均超过10000,远高于2020年版《中国药典》规定标准。峨眉山天麻中主要活性成分天麻素含量比较高,有很高的药用价值和资源开发利用价值。

工业污水处理厂环境应急污泥危险特性快速鉴别技术研究

本文以某工业园区污水处理厂环境应急事件污泥为研究对象,通过简单溯源分析和样品检测初筛工作,初判污泥主要有害成分为含镍有毒物质,主要危险特性为毒性,后对所采集的样品进行有针对性的镍含量检测,最终判定污泥为含镍废物,危险废物代码为900-000-26。该技术方法避免了传统固体废物危险特性鉴别方案编制工作程序,减少了样品检测指标,具有鉴别时间短、鉴别结论准、鉴别成本低等优点,为环境应急事件中固体废物危险特性鉴别提供了新思路。

Systematic analysis of components and contents in Houttuynia cordata Thunb

Objective:This study aims to provide the material basis for the molecular mechanism of Yuxingcao(Houttuynia cordata Thunb,HCT)through the method of evidence-based study to summarize the natural constituents isolated from HCT.Methods:We searched CNKI,Wanfang,VIP,Pubmed and relevant conference compilations.The keywords were'Yuxingcao or Houttuynia cordata Thunb'and'components'or'ingredients'or'constituent'or'volatile oil'or'flavonoids'or'terpene'or'content',both in Chinese and English.According to the inclusion and exclusion criteria,the reported compositions and contents have been summarized,and SPSS software was used to draw the boxplot of contents.Results:A total of 603 natural compounds in 11 categories were obtained from pooled articles.In the diverse components,the number of aliphatic compounds(n=259)and terpenoids(n=158)are more than those of flavonoids(n=26),alkaloids(n=42)and aromatics(n=42).While,in the part of volatile oils of HCT,the largest components are aliphatic compounds(mainly distributed on the ground)and terpenoids(mainly distributed in the underground).Although,methyl n-nonylketone is distributed in the whole herb plant,a large proportion is present in the underground parts.As for non-volatiles,the flavonoid content(mainly distributed on the ground)was the highest,among which quercetin and its glycosyl derivatives were the prominent.Conclusion:The results of this study provide a more comprehensive material basis for the further study of HCT.It is also helpful to explain the mechanisms of anti-oxidant,anti-inflammatory,anti-bacterial,anti-viral and anti-cancer effects from the molecular target and molecular network levels.

Content Analysis of Baicalin and Heavy Metals in Scutellaria baicalensis Georgi Cultivated in Jizhou District

This study aimed to explore an analysis method for baicalin in Scutellaria baicalensis Georgi and make a reference for the analysis of the medicinal value of S. baicalensis cultivated in Jizhou District. The content of baicalin in S. baicalensis cultivated in Jizhou District was determined using high-performance liquid chromatography( HPLC) to judge whether it met the requirements of Chinese Pharmacopoeia; and according to the requirements of the Chinese Pharmacopoeia of 2015 edition,the contents of heavy metals were determined by ICP-MS. The results showed that the contents of baicalin,Cr,Cu,As,Cd and Pb in S. baicalensis planted in Jizhou District were 14. 625 05%,11. 360,12. 830,0. 062 315,0. 025 715 and 0. 176 65 μg/g,respectively. The content of baicalin in S. baicalensis planted in Jizhou District was much higher than the standard( ≥ 9. 0%) of Chinese Pharmacopoeia of 2015 edition,and the contents of heavy metals were also below the limit( ≤20/1000 000) required by Chinese Pharmacopoeia of 2015 edition.