Objective:To investigate a hippocampal anti-hypertensive mechanism induced by twirling reinforcingreducing manipulation(TRRM)using proteomics in rats.Methods:Forty-two male spontaneously hypertensive rats were randoml...Objective:To investigate a hippocampal anti-hypertensive mechanism induced by twirling reinforcingreducing manipulation(TRRM)using proteomics in rats.Methods:Forty-two male spontaneously hypertensive rats were randomly divided into 3 groups,and 14 Wistar-Kyoto rats were served as control group.In the twirling reinforcing(TRF)group,needles were directly inserted into the Taichong(LR 3)point,then thumbs were moved heavily forward and lightly backward for 3 min,while needles remained inserted for 17 min.In the twirling reduction(TRD)group,the same treatment was applied as in the TRF group except that the thumb moved lightly forward and heavily backward.In the model and control groups,only the corresponding grasping and fixation were applied.All interventions were conducted for 14 days.The blood pressure(BP)of all rats was measured one day before intervention and every other day after.Then hematoxylin-eosin(H&E)staining,label-free and parallel reaction monitoring proteomic techniques were used to assess hippocampal samples from each group.Results:Systolic BPs showed a significant decrease in the TRF and TRD groups compared with the model group(P<.01).In the model group,H&E staining showed obvious pathological changes in the hippocampus,while in the TRF and TRD groups,the hippocampal morphology was only slightly altered.Labelfree proteomic analysis revealed 1163 differential protein expressions between groups.Gene Ontology enrichment analysis confirmed that the differentially expressed proteins were enriched in different biological processes,cellular components,and molecular functions.Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that TRRM proteins were expressed in the serotonergic synapse pathway,renin-angiotensin system,the mitogen-activated protein kinase signaling pathway,and the peroxisome pathway,which were all also related to BP regulation.Conclusion:TRRM can significantly lower the BP of SHRs.The mode of action may be through the activation of various protein pathways in the hippocampus that are related to BP regulation.展开更多
Contralateral needling method is a common and effective one of acupuncture analgesicmethods.It is used to treat a local pain in the limbs and trunk with a short course of disease,pain and swelling will disappear or be...Contralateral needling method is a common and effective one of acupuncture analgesicmethods.It is used to treat a local pain in the limbs and trunk with a short course of disease,pain and swelling will disappear or be relieved immediately for one or two treatments.Really,there isan effect of relieving pain as soon as the needle is inserted.When the local contralateral needlingmethod is used,the key to improving the effectiveness lies in determining the pain position and its correspondingpoint correctly,the more correct the pain position and its corresponding point are determined,the better the effectiveness will be.In this group,80 cases soft tissue injury were treatedwith local contralateral needling method,the cure rate was 82％,and the total effective rate was100％.展开更多
Objective：To observe the effect of complex reinforcing [Shao Shan Huo （Mountain-burning Fire）] and reducing [Tou Tian Liang （Heaven-penetrating Cooling）] manipulations on motion and balance of the lower limbs in ...Objective：To observe the effect of complex reinforcing [Shao Shan Huo （Mountain-burning Fire）] and reducing [Tou Tian Liang （Heaven-penetrating Cooling）] manipulations on motion and balance of the lower limbs in patients with subacute combined degeneration of the spinal cord （SCD）. Methods：A total of 100 SCD cases were randomly allocated into an observation group and a control group by their visit sequence, 50 cases in each group. On the basis of Western medical treatment, cases in the observation group were also treated with acupuncture therapy plus complex reinforcing-reducing manipulation; whereas cases in the control group were only treated with the same Western medical treatment as those in the observation group. Before and 2 months after treatment, kinematic parameters including muscle force, muscle tone and range of motion of the hip and knee joints were measured. In addition, balancing parameters including the path length of center of pressure （COP）, peripheral area, COP path length per unit area and rectangular area were also measured before and after treatment. Results：After treatment, there were statistical intra-group differences in COP path length, peripheral area, COP path length per unit area, left-right offset, rectangular area, coefficient of stability and coefficient of weight distribution （P〈0.01）. There were statistical inter-group differences in muscle force, muscle tone and range of motion of hip and knee joints （P〈0.01）. The total effective rate was 83.3% in the observation group, versus 60.0% in the control group, showing a statistical difference （P〈0.05）. Conclusion：Complex reinforcing-reducing manipulations can improve the lower limb motion and balance in SCD patients.展开更多
Objective： To observe the effect of complex reinforcing-reducing manipulations of acupuncture on flexion and extension angles of hip and knee in patients after surgical treatment of gluteus maximus contracture. 〈br...Objective： To observe the effect of complex reinforcing-reducing manipulations of acupuncture on flexion and extension angles of hip and knee in patients after surgical treatment of gluteus maximus contracture. 〈br〉 Methods： A total of 66 cases following surgery of gluteus maximus contracture were randomly allocated into an observation group and a control group by the random digits table, 33 in each group. In addition to basic treatment, cases in the observation group were treated with reducing manipulation [Tou T ian Liang （Heaven-penetrating Cooling）] on Zhibian （BL 54）, Huantiao （GB 30） and Juliao （GB 29） on the affected side and reinforcing manipulation [Shao Shan Huo （Mountain-burning Fire）] on Biguan （ST 31）, Futu （ST 32）, Zusanli （ST 36）, Fenglong （ST 40）, Xuehai （SP 10）, Diji （SP 8） and Yanglingquan （GB 34）. Patients in the control group only received the same basic treatment as the observation group. The treatment was done once a day, 30 d for a course and a 1-week interval between two courses. Then the flexion and extension angles of hip and knee were evaluated using the Lokomat full automatic robot gait evaluation system after 3 courses of treatment. 〈br〉 Results：There were intra-group statistical differences in hip flexion angle on foot followed （HFA-FF）, the maximum of hip flexion angle （MAX-HFA）, the maximum of hip extension angle （MAX-HEA）, knee flexion angle on foot followed （KFA-FF）, the maximum of knee flexion angle on stance phase （MAX-KFA-TP） and the maximum of knee flexion angle on swing phase （MAX-KFA-WP） in the observation group and in HFA-FF, MAX-HEA and KFA-FF in the control group （P＆lt;0.05）. There were between-group statistical differences in HFA-FF, MAX-HFA, MAX-HEA, KFA-FF and MAX-KFA-TP （P＆lt;0.05）. 〈br〉 Conclusion： The complex reinforcing-reducing manipulations of acupuncture can effectively improve the hip/knee functions following surgery of gluteus maximus contracture.展开更多
Objective： To observe the clinical effect of lower-reinforcing and upper-reducing acupuncture method for hyperplasia of mammary gland （HMG） and its influence on estradiol （E2） and progesterone （P）. Methods： A ...Objective： To observe the clinical effect of lower-reinforcing and upper-reducing acupuncture method for hyperplasia of mammary gland （HMG） and its influence on estradiol （E2） and progesterone （P）. Methods： A randomized, single-blinded and controlled trial was conducted. A total of 124 cases conforming to the inclusion criteria were randomized by random number table into a treatment group and a control group, with 62 cases in each group. Patients in both groups received acupuncture therapy at the same acupoints, while patients in the treatment group received lower-reinforcing and upper-reducing method, and patients in the control group received even reinforcing-reducing manipulation. The treatment started around 10 d before menstruation and was conducted every other day. Patients received 5 treatments in each menstruation cycle for consecutive 3 cycles. The levels of E2, P and E2/P and clinical efficacy were measured before and after treatment. Results： After treatment, the breast lump size, pain intensity and concomitant symptoms score in both groups were substantially lower than those before treatment, showing statistical significances （all P〈0.01）, and the improvement in the treatment group was higher than that in the control group, and the between-group comparisons showed statistical significances （all P〈0.01）. After treatment, the overall effective rate was 91.9% in the treatment group, higher than 72.6% in the control group, and the between-group comparison showed a statistical significance （P〈0.01）. After treatment, levels of E2, P and E2/P value showed no statistical significance when compared with those before treatment in the two groups （all P〉0.05）. Conclusion： Lower-reinforcing and upper-reducing acupuncture method can effectively alleviate clinical symptoms and signs in HMG patients, and produce a better effect than even reinforcing-reducing manipulation. The majority of HMG patients＇ E2, P level and E2/P value were not beyond the normal ranges; therefore, acupuncture showed no substantial influence on E2 and P levels and E2/P value.展开更多
基金supported by the National Natural Science Foundation of China(82074553)the Qilu Traditional Chinese Medicine Oriented Specialty Cluster,Rehabilitation Group 2.
文摘Objective:To investigate a hippocampal anti-hypertensive mechanism induced by twirling reinforcingreducing manipulation(TRRM)using proteomics in rats.Methods:Forty-two male spontaneously hypertensive rats were randomly divided into 3 groups,and 14 Wistar-Kyoto rats were served as control group.In the twirling reinforcing(TRF)group,needles were directly inserted into the Taichong(LR 3)point,then thumbs were moved heavily forward and lightly backward for 3 min,while needles remained inserted for 17 min.In the twirling reduction(TRD)group,the same treatment was applied as in the TRF group except that the thumb moved lightly forward and heavily backward.In the model and control groups,only the corresponding grasping and fixation were applied.All interventions were conducted for 14 days.The blood pressure(BP)of all rats was measured one day before intervention and every other day after.Then hematoxylin-eosin(H&E)staining,label-free and parallel reaction monitoring proteomic techniques were used to assess hippocampal samples from each group.Results:Systolic BPs showed a significant decrease in the TRF and TRD groups compared with the model group(P<.01).In the model group,H&E staining showed obvious pathological changes in the hippocampus,while in the TRF and TRD groups,the hippocampal morphology was only slightly altered.Labelfree proteomic analysis revealed 1163 differential protein expressions between groups.Gene Ontology enrichment analysis confirmed that the differentially expressed proteins were enriched in different biological processes,cellular components,and molecular functions.Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that TRRM proteins were expressed in the serotonergic synapse pathway,renin-angiotensin system,the mitogen-activated protein kinase signaling pathway,and the peroxisome pathway,which were all also related to BP regulation.Conclusion:TRRM can significantly lower the BP of SHRs.The mode of action may be through the activation of various protein pathways in the hippocampus that are related to BP regulation.
文摘Contralateral needling method is a common and effective one of acupuncture analgesicmethods.It is used to treat a local pain in the limbs and trunk with a short course of disease,pain and swelling will disappear or be relieved immediately for one or two treatments.Really,there isan effect of relieving pain as soon as the needle is inserted.When the local contralateral needlingmethod is used,the key to improving the effectiveness lies in determining the pain position and its correspondingpoint correctly,the more correct the pain position and its corresponding point are determined,the better the effectiveness will be.In this group,80 cases soft tissue injury were treatedwith local contralateral needling method,the cure rate was 82％,and the total effective rate was100％.
基金supported by Scientific Research Project of Hebei Provincial Administration Bureau of Traditional Chinese Medicine,No.2014188Hebei Tangshan Science and Technology Project,No.121302118b~~
文摘Objective：To observe the effect of complex reinforcing [Shao Shan Huo （Mountain-burning Fire）] and reducing [Tou Tian Liang （Heaven-penetrating Cooling）] manipulations on motion and balance of the lower limbs in patients with subacute combined degeneration of the spinal cord （SCD）. Methods：A total of 100 SCD cases were randomly allocated into an observation group and a control group by their visit sequence, 50 cases in each group. On the basis of Western medical treatment, cases in the observation group were also treated with acupuncture therapy plus complex reinforcing-reducing manipulation; whereas cases in the control group were only treated with the same Western medical treatment as those in the observation group. Before and 2 months after treatment, kinematic parameters including muscle force, muscle tone and range of motion of the hip and knee joints were measured. In addition, balancing parameters including the path length of center of pressure （COP）, peripheral area, COP path length per unit area and rectangular area were also measured before and after treatment. Results：After treatment, there were statistical intra-group differences in COP path length, peripheral area, COP path length per unit area, left-right offset, rectangular area, coefficient of stability and coefficient of weight distribution （P〈0.01）. There were statistical inter-group differences in muscle force, muscle tone and range of motion of hip and knee joints （P〈0.01）. The total effective rate was 83.3% in the observation group, versus 60.0% in the control group, showing a statistical difference （P〈0.05）. Conclusion：Complex reinforcing-reducing manipulations can improve the lower limb motion and balance in SCD patients.
基金supported by Tangshan City Science&Technology Program(No.121302118b)
文摘Objective： To observe the effect of complex reinforcing-reducing manipulations of acupuncture on flexion and extension angles of hip and knee in patients after surgical treatment of gluteus maximus contracture. 〈br〉 Methods： A total of 66 cases following surgery of gluteus maximus contracture were randomly allocated into an observation group and a control group by the random digits table, 33 in each group. In addition to basic treatment, cases in the observation group were treated with reducing manipulation [Tou T ian Liang （Heaven-penetrating Cooling）] on Zhibian （BL 54）, Huantiao （GB 30） and Juliao （GB 29） on the affected side and reinforcing manipulation [Shao Shan Huo （Mountain-burning Fire）] on Biguan （ST 31）, Futu （ST 32）, Zusanli （ST 36）, Fenglong （ST 40）, Xuehai （SP 10）, Diji （SP 8） and Yanglingquan （GB 34）. Patients in the control group only received the same basic treatment as the observation group. The treatment was done once a day, 30 d for a course and a 1-week interval between two courses. Then the flexion and extension angles of hip and knee were evaluated using the Lokomat full automatic robot gait evaluation system after 3 courses of treatment. 〈br〉 Results：There were intra-group statistical differences in hip flexion angle on foot followed （HFA-FF）, the maximum of hip flexion angle （MAX-HFA）, the maximum of hip extension angle （MAX-HEA）, knee flexion angle on foot followed （KFA-FF）, the maximum of knee flexion angle on stance phase （MAX-KFA-TP） and the maximum of knee flexion angle on swing phase （MAX-KFA-WP） in the observation group and in HFA-FF, MAX-HEA and KFA-FF in the control group （P＆lt;0.05）. There were between-group statistical differences in HFA-FF, MAX-HFA, MAX-HEA, KFA-FF and MAX-KFA-TP （P＆lt;0.05）. 〈br〉 Conclusion： The complex reinforcing-reducing manipulations of acupuncture can effectively improve the hip/knee functions following surgery of gluteus maximus contracture.
文摘Objective： To observe the clinical effect of lower-reinforcing and upper-reducing acupuncture method for hyperplasia of mammary gland （HMG） and its influence on estradiol （E2） and progesterone （P）. Methods： A randomized, single-blinded and controlled trial was conducted. A total of 124 cases conforming to the inclusion criteria were randomized by random number table into a treatment group and a control group, with 62 cases in each group. Patients in both groups received acupuncture therapy at the same acupoints, while patients in the treatment group received lower-reinforcing and upper-reducing method, and patients in the control group received even reinforcing-reducing manipulation. The treatment started around 10 d before menstruation and was conducted every other day. Patients received 5 treatments in each menstruation cycle for consecutive 3 cycles. The levels of E2, P and E2/P and clinical efficacy were measured before and after treatment. Results： After treatment, the breast lump size, pain intensity and concomitant symptoms score in both groups were substantially lower than those before treatment, showing statistical significances （all P〈0.01）, and the improvement in the treatment group was higher than that in the control group, and the between-group comparisons showed statistical significances （all P〈0.01）. After treatment, the overall effective rate was 91.9% in the treatment group, higher than 72.6% in the control group, and the between-group comparison showed a statistical significance （P〈0.01）. After treatment, levels of E2, P and E2/P value showed no statistical significance when compared with those before treatment in the two groups （all P〉0.05）. Conclusion： Lower-reinforcing and upper-reducing acupuncture method can effectively alleviate clinical symptoms and signs in HMG patients, and produce a better effect than even reinforcing-reducing manipulation. The majority of HMG patients＇ E2, P level and E2/P value were not beyond the normal ranges; therefore, acupuncture showed no substantial influence on E2 and P levels and E2/P value.
