Noni juice is not hepatotoxic

Noni juice （Morinda citrifolia） has been approved for use as a safe food within the European Union, following a review of safety. Since approval, three cases of acute hepatitis in Austrian noni juice consumers have been published, where a causal link is suggested between the liver dysfunction and ingestion of anthraquinones from the plant. Measurements of liver function in a human clinical safety study of TAHITIAN NONI Juice, as well as subacute and subchronic animal toxicity tests revealed no evidence of adverse liver effects at doses many times higher than those reported in the case studies. Additionally, M. citrifolia anthraquinones occur in the fruit in quantities too small to be of any toxicological significance. Further, these do not have chemical structures capable of being reduced to reactive anthrone radicals, which were implicated in previous cases of herbal hepototoxicity. The available data reveals no evidence of liver toxicity.

Consumer Perceptions of Noni Juice Health Benefits during a 90-Day In-Home Use Test

Noni juice, made from the fruit of Morinda citrifolia, is a health-promoting food from the Pacific Islands with an extensive history of traditional use. Human clinical trials have revealed that noni juice has antioxidant, immunomodulatory and anti-inflammatory properties. Previously, regular noni juice consumers in Europe and traditional healers in Fiji were surveyed to identify potential noni juice health benefits. But to better understand the perception of its health benefits among those with no previous experience with noni juice, an in-home study was conducted in the United States. Three hundred and nineteen adults with untreated chronic health conditions were provided with a 90-day supply of a blinded (unlabeled) commercial noni juice product (Tahitian Noni® Juice), instructed to consume 4 fluid ounces (1/2 cup) each day, and responded to a questionnaire given on days 30, 60 and 90 to evaluate product appeal and perceived effectiveness. A total of 299 people (94%) felt that their health improved in some way after drinking TNJ for 90 days. More than 80% of the subjects reported feeling better or healthier in general. Most participants also concurred with more than two dozen additional positive health perception statements, especially on days 60 and 90. More than 70% of consumers also indicated that noni juice helped them feel better than other wellness supplements they had tried in the past, including other so-called “super fruit” products. These findings seem to corroborate the results of clinical trials and previous consumer surveys regarding the potential for noni juice to provide discernable health benefits when used consistently over time. Such findings may help guide additional placebo-controlled clinical trials in which specific effects may be evaluated more rigorously.

The Influence of Noni Fruit Juice on Immune System Function

Morinda citrifolia (noni) fruit juice has the potential to influence immune system function. This review discusses results from several human and animal studies that provide insight into the potential mechanisms of action by which noni juice exerts its immunomodulatory effects. Increased natural killer cell activity is a likely a major contributor to the improved health outcomes and increased survival times described in case reports and as observed in LLC and S180 tumor bearing mice. Increased interferon-gamma (IFN-γ) production is also an important mechanism of action through which noni improves immune function. IFN-γ promotes natural killer cell activity and phagocytosis, activities both seen in human and bovine studies as well as in rodents. Noni promotes regulatory cytokine expression, such as IL-2 which stimulates CD4+ T cell differentiation. Noni juice appears to influence this process via kinase 1/2 (ERK1/2), protein kinase B (Akt) and nuclear factor-kappa-beta signaling. As oxidative status is known to influence immune function, this review also discusses the notable antioxidant properties of noni juice that have been demonstrated in human trials.

Morinda citrifolia(Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway

Objective:To study the mechanism of the anti-tumor effect of Morinda citrifolia(noni).Methods:The influences of noni juice on cell proliferation,apoptosis,invasion,migration and the activity of AKT/nuclear factor-κB(NF-κB)signaling pathway in A549 human lung cancer cells were detected by MTT,cell counting kit-8,colony formation,Annexin V/PI double labeling,transwel,scratch test and immunoblotting assay,respectively.A549 cells were inoculated into the right axilla of nude mice,followed by noni juice treatment.The body weight of the nude mice was weighed,and the tumor volume and weight were measured.Cel proliferation and expression of apoptosis-related proteins were measured by immunohistochemistry,and the activity of NF-κB signaling pathway was measured by immunoblotting.Results:The in vitro studies showed that noni juice inhibited the A549 cel s proliferation,migration and invasion.Noni juice also promoted cel s apoptosis in A549 cel s.Immunoblotting assay showed that the phosphorylation level of AKT,p50,and STAT3 proteins was inhibited to different extents after noni juice treatment.The in vivo studies showed that noni juice effectively suppressed tumor formation of A549 cells in nude mice.Noni juice treatment inhibited the expression of Ki67,PCNA,and Bcl-2 protein in the tumor;while promoted the expression of caspase-3 protein.Additionally,we also found that noni juice treatment could restrain the activity of AKT/NF-κB signaling pathway in the tumor tissue.Conclusion:Noni juice inhibited the proliferation of A549 lung cancer cells,induced apoptosis,and inhibited cel invasion and migration via regulating AKT/NF-κB signaling pathway.

HPLC法测定库克Noni果汁中游离态水溶性维生素

采用HPLC法测定Non i果汁中游离态的抗坏血酸(Vc)、硫胺素(VB1)和烟酰胺(VB5),其测定条件为:色谱柱为A lltim a C18柱,流动相为甲醇-0.01%磷酸(3:7,V/V),流速为1.0 mL/m in,进样量为20μL,柱温为室温,采用二极管阵列检测器在251 nm下检测。在上述色谱测定条件下三种维生素能得到良好分离。结果表明,该方法灵敏度高,定量准确,重现性好,回收率高。所测Non i果汁中三种维生素的含量分别为:Vc 0.354 mg/mL,VB10.066 3 mg/mL,VB50.131 mg/mL。说明Non i果汁中三种必需维生素含量丰富,可作为维生素缺乏的营养补充剂;所建立的测定方法可作为一种Non i果汁生产质量控制方法。

库克诺你果汁提取物体外清除自由基及抗氧化活性研究(英文)

本文对诺你果汁多糖、乙醇溶出物和乙酸乙酯萃取物体外对超氧阴离子(O2·)、羟自由基(·OH)、DPPH和脂质过氧化(LPO)的抑制作用进行了研究。超氧阴离子(O2·)由邻苯三酚自氧化产生;羟自由基(·OH)由Fenton反应产生;利用Fe2+诱发卵黄脂蛋白产生丙二醛(MDA),TBA法测定。所有测定均为分光光度法。结果表明,与已知抗氧化剂L抗坏血酸相比,乙醇溶出物和乙酸乙酯萃取物均有明显的捕捉自由基和抗氧化能力,而多糖捕捉自由基和抗氧化能力很低,且对O2·没有抑制作用,反而会增加其生成速度。

诺丽自然发酵汁中醋酸菌的分离鉴定及发酵特性

为分析诺丽自然发酵汁中醋酸菌的多样性,丰富诺丽中醋酸菌种属信息,采用传统分离培养和16S rRNA基因序列分析相结合的方法,对诺丽自然发酵汁中的醋酸菌进行分离鉴定和发酵特性研究。结果表明,从不同发酵阶段诺丽自然发酵汁的67 个分离菌株中鉴定出了24 株醋酸菌,分别为Acetobacter fabarum(9 株)、Acetobacter syzygii(7 株)、Acetobacter pasteurianus(2 株)、Acetobacter tropicalis(1 株)、Acetobacter lambici(1 株)和Gluconobacter japonicus(4 株),其中A. syzygii和A. fabarum为诺丽自然发酵过程中的优势菌种。在发酵性能方面,A. tropicalis N21性能最优,产酸量可达28.92 g/L,在40 ℃高温中仍能良好生长且产酸量为14.85 g/L,乙醇体积分数为7%时,产酸量略有下降,但依然可达23.60 g/L,其耐高温和耐乙醇能力均高于其他菌株。