Non-invasive tracing in vivo can be used to observe the migration and distnbution of grafted stem cells, and can provide experimental evidence for treatment. This study utilized adenovirus-carrying enhanced green fluo...Non-invasive tracing in vivo can be used to observe the migration and distnbution of grafted stem cells, and can provide experimental evidence for treatment. This study utilized adenovirus-carrying enhanced green fluorescent protein （AD5/F35-eGFP） and superparamagnetic iron oxide （SPIO）-Iabeled bone marrow mesenchymal stem cells （BMSCs）. BMSCs, double-labeled by AD5/F35-eGFP and SPIO, were transplanted into rats with spinal cord injury via the subarachnoid space. MRI tracing results demonstrated that BMSCs migrated to the injured spinal cord over time （T2 hypointensity signals）. This result was verified by immunofluorescence. These results indicate that MRI can be utilized to trace in vivo the SPIO-labeled BMSCs after grafting.展开更多
This study aimed to identify the optimal neural progenitor cell transplantation time for spinal cord injury in rats via the subarachnoid space. Cultured neural progenitor cells from 14-day embryonic rats, constitutive...This study aimed to identify the optimal neural progenitor cell transplantation time for spinal cord injury in rats via the subarachnoid space. Cultured neural progenitor cells from 14-day embryonic rats, constitutively expressing enhanced green fluorescence protein, or media alone, were injected into the subarachnoid space of adult rats at 1 hour （acute stage）, 7 days （subacute stage） and 28 days （chronic stage） after contusive spinal cord injury. Results showed that grafted neural progenitor cells migrated and aggregated around the blood vessels of the injured region, and infiltrated the spinal cord parenchyma along the tissue spaces in the acute stage transplantation group. However, this was not observed in subacute and chronic stage transplantation groups. 04- and glial fibrillary acidic protein-positive cells, representing oligodendrocytes and astrocytes respectively, were detected in the core of the grafted cluster attached to the cauda equina pia surface in the chronic stage transplantation group 8 weeks after transplantation. Both acute and subacute stage transplantation groups were negative for 04 and glial fibrillary acidic protein cells. Basso, Beattie and Bresnahan scale score comparisons indicated that rat hind limb locomotor activity showed better recovery after acute stage transplantation than after subacute and chronic transplantation. Our experimental findings suggest that the subarachnoid route could be useful for transplantation of neural progenitor cells at the acute stage of spinal cord injury. Although grafted cells survived only for a short time and did not differentiate into astrocytes or neurons, they were able to reach the parenchyma of the injured spinal cord and improve neurological function in rats. Transplantation efficacy was enhanced at the acute stage in comparison with subacute and chronic stages.展开更多
Extra-nasopharyngeal lymphoepithelioma-like carcinomas (LELC) are uncommon epithelial tumors. A few isolated case reports and series are available in literature involving the larynx, pharynx, salivary gland, lung etc....Extra-nasopharyngeal lymphoepithelioma-like carcinomas (LELC) are uncommon epithelial tumors. A few isolated case reports and series are available in literature involving the larynx, pharynx, salivary gland, lung etc., but involvement in the parapharyngeal space has not yet been reported. We aim to highlight one such case that has a clinical and radiological characteristic of a benign lesion whilst the histopathology reveals an infiltrating neoplasm. The typical clinical aggressiveness of a classical LELC of extra-nasopharyngeal lesion as described in literature is not present in our case. Also seen is an uncommon finding of abnormal branching of left external carotid artery. There is no standard treatment protocol for such a tumor;however, wide excision of this tumor in the parapharyngeal space via trans-cervical, trans-mandibular, trans-palatal approach has shown good and satisfactory tumor control of the primary site so far.展开更多
基金the National Natural Science Foundation of China,No.81000530, 30973093the Creative Talent Project of Henan Province Health Department, No.2010-4106
文摘Non-invasive tracing in vivo can be used to observe the migration and distnbution of grafted stem cells, and can provide experimental evidence for treatment. This study utilized adenovirus-carrying enhanced green fluorescent protein （AD5/F35-eGFP） and superparamagnetic iron oxide （SPIO）-Iabeled bone marrow mesenchymal stem cells （BMSCs）. BMSCs, double-labeled by AD5/F35-eGFP and SPIO, were transplanted into rats with spinal cord injury via the subarachnoid space. MRI tracing results demonstrated that BMSCs migrated to the injured spinal cord over time （T2 hypointensity signals）. This result was verified by immunofluorescence. These results indicate that MRI can be utilized to trace in vivo the SPIO-labeled BMSCs after grafting.
基金supported by the People’s Liberation Army Fund for Special Projects of Medical Sciences, No. 06G114
文摘This study aimed to identify the optimal neural progenitor cell transplantation time for spinal cord injury in rats via the subarachnoid space. Cultured neural progenitor cells from 14-day embryonic rats, constitutively expressing enhanced green fluorescence protein, or media alone, were injected into the subarachnoid space of adult rats at 1 hour （acute stage）, 7 days （subacute stage） and 28 days （chronic stage） after contusive spinal cord injury. Results showed that grafted neural progenitor cells migrated and aggregated around the blood vessels of the injured region, and infiltrated the spinal cord parenchyma along the tissue spaces in the acute stage transplantation group. However, this was not observed in subacute and chronic stage transplantation groups. 04- and glial fibrillary acidic protein-positive cells, representing oligodendrocytes and astrocytes respectively, were detected in the core of the grafted cluster attached to the cauda equina pia surface in the chronic stage transplantation group 8 weeks after transplantation. Both acute and subacute stage transplantation groups were negative for 04 and glial fibrillary acidic protein cells. Basso, Beattie and Bresnahan scale score comparisons indicated that rat hind limb locomotor activity showed better recovery after acute stage transplantation than after subacute and chronic transplantation. Our experimental findings suggest that the subarachnoid route could be useful for transplantation of neural progenitor cells at the acute stage of spinal cord injury. Although grafted cells survived only for a short time and did not differentiate into astrocytes or neurons, they were able to reach the parenchyma of the injured spinal cord and improve neurological function in rats. Transplantation efficacy was enhanced at the acute stage in comparison with subacute and chronic stages.
文摘Extra-nasopharyngeal lymphoepithelioma-like carcinomas (LELC) are uncommon epithelial tumors. A few isolated case reports and series are available in literature involving the larynx, pharynx, salivary gland, lung etc., but involvement in the parapharyngeal space has not yet been reported. We aim to highlight one such case that has a clinical and radiological characteristic of a benign lesion whilst the histopathology reveals an infiltrating neoplasm. The typical clinical aggressiveness of a classical LELC of extra-nasopharyngeal lesion as described in literature is not present in our case. Also seen is an uncommon finding of abnormal branching of left external carotid artery. There is no standard treatment protocol for such a tumor;however, wide excision of this tumor in the parapharyngeal space via trans-cervical, trans-mandibular, trans-palatal approach has shown good and satisfactory tumor control of the primary site so far.
