To obtain the recombinant core domain of porcine zone pellucida 3β （cZP3β） for the further research on its functions Methods The nucleotide sequence region from 44 to 306 codons of pZP3β entire eDNA was obtained ...To obtain the recombinant core domain of porcine zone pellucida 3β （cZP3β） for the further research on its functions Methods The nucleotide sequence region from 44 to 306 codons of pZP3β entire eDNA was obtained by PCR and then was cloned into pET-3c vector. After being identified, recon was transformed into E.coli BL21 （DE3） pLysS and then induced by IPTG. Results The recombinant cZP3β was expressed in E. coli up to 15% of total cellular proteins, and was made sure by Western blot analysis. Conclusion The research on expression of core domain of pZP3β could benefit to further investigation of its immunogenicity and the development of antigen preparation.展开更多
Integrase plays a critical role in the recombination of viral DNA into the host genome.Therefore,over the past decade,it has been a hot target of drug design in the fight against type 1 human immunodeficiency virus(HI...Integrase plays a critical role in the recombination of viral DNA into the host genome.Therefore,over the past decade,it has been a hot target of drug design in the fight against type 1 human immunodeficiency virus(HIV-1).Bovine immunodeficiency virus(BIV)integrase has the same function as HIV-1 integrase.We have determined crystal structures of the BIV integrase catalytic core domain(CCD)in two different crystal forms at a resolution of 2.45Åand 2.2Å,respectively.In crystal form I,BIV integrase CCD forms a back-to-back dimer,in which the two active sites are on opposite sides.This has also been seen in many of the CCD structures of HIV-1 integrase that were determined previously.However,in crystal form II,BIV integrase CCD forms a novel face-toface dimer in which the two active sites are close to each other.Strikingly,the distance separating the two active sites is approximately 20Å,a distance that perfectly matches a 5-base pair interval.Based on these data,we propose a model for the interaction of integrase with its target DNA,which is also supported by many published biochemical data.Our results provide important clues for designing new inhibitors against HIV-1.展开更多
区分配电网中发生的单相接地故障类型,能够有针对性地制定故障检修策略,提升故障处置效率。配电自动化设备作为配电网故障快速辨识与处理的重要载体,对故障分类的原理及效果差异性较大,准确率无法满足电力系统工作需求,为此提出一种基...区分配电网中发生的单相接地故障类型,能够有针对性地制定故障检修策略,提升故障处置效率。配电自动化设备作为配电网故障快速辨识与处理的重要载体,对故障分类的原理及效果差异性较大,准确率无法满足电力系统工作需求,为此提出一种基于分类回归树与多核残差网络(classfication and regression tree and multi-core ResNet, CART-MRN)的树状结构故障类型识别方法。首先,建立树状故障分类结构,利用Fourier变换、经验模态分解(empirical mode decompsition, EMD)分解等方法提取故障点电压电流的多域故障特征;其次,结合特征分析与信息增益建立适应不同小电流接地系统的融合算法模型,并引入粒子群算法优化网络超参数;最后,通过现场录波数据验证与对比实验,证明该方法能快速、有效地完成单相接地故障分类识别,且更具有适应性。展开更多
基金This study was supported by the Science & Technology Plan (No. 2001C12001) of Guangdong Province,P.R. China
文摘To obtain the recombinant core domain of porcine zone pellucida 3β （cZP3β） for the further research on its functions Methods The nucleotide sequence region from 44 to 306 codons of pZP3β entire eDNA was obtained by PCR and then was cloned into pET-3c vector. After being identified, recon was transformed into E.coli BL21 （DE3） pLysS and then induced by IPTG. Results The recombinant cZP3β was expressed in E. coli up to 15% of total cellular proteins, and was made sure by Western blot analysis. Conclusion The research on expression of core domain of pZP3β could benefit to further investigation of its immunogenicity and the development of antigen preparation.
基金This study was supported by grants from the Ministry of Science and Technology of China(Grant Nos.2007CB914301,2009CB825504,2006AA02A319,2006AA020502)the Ministry of Health of China(Grant No.2008ZX10001-002)+1 种基金the National Natural Science Foundation of China(Grant Nos.30770428,30940015)the TBR programs(Grant Nos.08QTPTJC28200,08SYSYTC00200,07JCYBJC19200).
文摘Integrase plays a critical role in the recombination of viral DNA into the host genome.Therefore,over the past decade,it has been a hot target of drug design in the fight against type 1 human immunodeficiency virus(HIV-1).Bovine immunodeficiency virus(BIV)integrase has the same function as HIV-1 integrase.We have determined crystal structures of the BIV integrase catalytic core domain(CCD)in two different crystal forms at a resolution of 2.45Åand 2.2Å,respectively.In crystal form I,BIV integrase CCD forms a back-to-back dimer,in which the two active sites are on opposite sides.This has also been seen in many of the CCD structures of HIV-1 integrase that were determined previously.However,in crystal form II,BIV integrase CCD forms a novel face-toface dimer in which the two active sites are close to each other.Strikingly,the distance separating the two active sites is approximately 20Å,a distance that perfectly matches a 5-base pair interval.Based on these data,we propose a model for the interaction of integrase with its target DNA,which is also supported by many published biochemical data.Our results provide important clues for designing new inhibitors against HIV-1.
文摘区分配电网中发生的单相接地故障类型,能够有针对性地制定故障检修策略,提升故障处置效率。配电自动化设备作为配电网故障快速辨识与处理的重要载体,对故障分类的原理及效果差异性较大,准确率无法满足电力系统工作需求,为此提出一种基于分类回归树与多核残差网络(classfication and regression tree and multi-core ResNet, CART-MRN)的树状结构故障类型识别方法。首先,建立树状故障分类结构,利用Fourier变换、经验模态分解(empirical mode decompsition, EMD)分解等方法提取故障点电压电流的多域故障特征;其次,结合特征分析与信息增益建立适应不同小电流接地系统的融合算法模型,并引入粒子群算法优化网络超参数;最后,通过现场录波数据验证与对比实验,证明该方法能快速、有效地完成单相接地故障分类识别,且更具有适应性。
