Analgesic and anti-inflammatory activity of Cotinus coggygria Scop.extracts in vivo

Objective:To assess the analgesic and anti-inflammatory effects of standardized extract of Cotinus coggygria(C.coggygria)in different animal models.Methods:C.coggygria extracts(25,50,and 100 mg/kg)were administered to rats and mice(n=6)during hot plate,tail-flick,acetic acid-induced writhing,and formalin tests to determine its analgesic efficacy.The anti-inflammatory activity of C.coggygria extracts was evaluated by histamine and carrageenan-induced paw edema,cotton pellet-induced granuloma,and acetic acid-induced peritoneal capillary dye leakage tests.Results:C.coggygria extracts(50 and 100 mg/kg)significantly alleviated thermal and chemical-induced pain in rodents(P<0.05).It also demonstrated notable anti-inflammatory properties by mitigating histamine and carrageenan-induced paw edema,granuloma deposits,and vascular permeability(P<0.05).Moreover,C.coggygria extracts remarkably reduced TNF-α,IL-1β,IL-6,COX-2,and oxidative stress in rat paws(P<0.05).Carrageenan-induced histological aberrations in hind paw tissues were effectively(P<0.05)mitigated by treatment with C.coggygria extracts.Conclusions:C.coggygria Scop.extracts show analgesic and anti-inflammatory effects via inhibition of COX-2 and inflammatory and oxidative mediators.

Control of verticillium wilt in Cotinus coggygria

We analyzed the inhibition rates of eight kinds of fungicides to the pathogen of verticillium wilt of smoke tree （Cotinus coggygria） by measuring mycelium growth. Results show that four fungicides （Weijunjing, thiophanate-methyl 70% WP （wettable powder）, carbendazim 50% WP and Junxianwei） have the best antifungal effects. Three fungicides, Weijunjing, carbendazim 50% WP and Junxianwei, were selected to determine the control of verticillium wilt in potted smoke tree seedlings. We found that the relative efficacy of Weijunjing was up to 74%. Based on the results, Weijunjing and carbendazim 50% WP were prepared for field trials. The results indicate that the relative efficacy of Weijunjing （300 times diluted） and carbendazim 50% WP （400 times diluted） were 66.2% and 48.9% in plot 1 and 26.4% and 31.8% in plot 2 after soil disinfection, and were 224% and 61.8% in plot 3 without soil disinfection in 2007. The relative efficacy of Weijunjing and carbendazim 50% WP were 49.6% and 45.4% in plot 2 in 2008.