New aspects of a small GTPase RAB35 in brain development and function

In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting the cargo of proteins,lipids,and other molecules to appropriate compartments via transport vesicles or intermediates.These processes are strictly regulated by various small GTPases such as the RAS-like in rat brain(RAB)protein family,which is the largest subfamily of the RAS superfamily.Dysfunction of membrane trafficking affects tissue homeostasis and leads to a wide range of diseases,including neurological disorders and neurodegenerative diseases.Therefore,it is important to understand the physiological and pathological roles of RAB proteins in brain function.RAB35,a member of the RAB family,is an evolutionarily conserved protein in metazoans.A wide range of studies using cultured mammalian cells and model organisms have revealed that RAB35 mediates various processes such as cytokinesis,endocytic recycling,actin bundling,and cell migration.RAB35 is also involved in neurite outgrowth and turnover of synaptic vesicles.We generated brain-specific Rab35 knockout mice to study the physiological roles of RAB35 in brain development and function.These mice exhibited defects in anxiety-related behaviors and spatial memory.Strikingly,RAB35 is required for the precise positioning of pyramidal neurons during hippocampal development,and thereby for normal hippocampal lamination.In contrast,layer formation in the cerebral cortex occurred superficially,even in the absence of RAB35,suggesting a predominant role for RAB35 in hippocampal development rather than in cerebral cortex development.Recent studies have suggested an association between RAB35 and neurodegenerative diseases,including Parkinson's disease and Alzheimer's disease.In this review,we provide an overview of the current understanding of subcellular functions of RAB35.We also provide insights into the physiological role of RAB35 in mammalian brain development and function,and discuss the involvement of RAB35 dysfunction in neurodegenerative diseases.

Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins

Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

Development Status and Countermeasures of Farmers' Professional Cooperatives in Shangqiu City

Farmers' professional cooperatives develop in various forms,and with the continuous improvement of development level,social effects have gradually appear.Based on the specific situation of farmers' professional cooperatives in Shangqiu City,this paper expounded the development status of farmers' professional cooperatives in Shangqiu City,analyzed and discussed various problems encountered in the development process of farmers' professional cooperatives,and put forward some countermeasures and suggestions,such as establishing and perfecting cooperative rules and regulations,financing from multiple channels,introducing technical talents in various ways,breaking the information asymmetry restriction by various means,and giving full play to government functions,aiming to further promote standardized,large-scale,healthy and sustainable development of farmers' professional cooperatives.

Pathway and Countermeasure of Farmers' Livelihood Transformation in the Ecological Development Area of Northern Guangdong Province from the Perspective of Ecological Protection

[Objectives]To explore pathways and countermeasures for transforming farmers livelihoods in the way of reducing their dependence on land while promoting sustainable development and alleviating ecological degradation.[Methods]A combination of field research,literature review,and policy analysis was employed to identify key factors affecting farmers livelihoods and potential strategies for transformation.[Results]The study found that developing ecological agriculture and modern agriculture,promoting agricultural transformation and upgrading,cultivating alternative industries,strengthening ecological engineering construction,and establishing diversified ecological compensation methods and supporting policies are effective strategies for transforming farmers livelihoods.[Conclusions]Implementing these strategies can help alleviate the contradiction between ecological protection and farmers livelihood development,promoting coordinated development of both.This approach not only benefits farmers but also contributes to sustainable environmental management and biodiversity conservation.

Current status and countermeasures of facility fishery development

According to the"National Modern Facility Agriculture Construction Plan(2023-2030)released by the Ministry of Agriculture and Rural Affairs,the"Special Implementation Plan for Modern Facility Fisheries Construction"emphasizes the development of circular aquaculture,active expansion of green aquaculture space in facility fisheries,multi-channel development of food sources,comprehensive improvement of the quality,efficiency,and competitiveness of facility fisheries,ensuring stable and safe supply of aquatic products,and better meeting people's needs for high-quality aquatic products and beautiful aquatic ecological environment.Since the implementation of the 14th Five Year Plan for fisheries,facility fisheries have made significant progress.In order to thoroughly implement General Secretary Xi Jinping's important discourse on new quality productivity,conduct in-depth research on the current situation and existing problems of the fishery industry,and put forward suggestions to meet the requirements of high-quality development of the fishery industry in the new era.Therefore,it is crucial to continue practicing the"revitalization of aquatic seed industry"and"big food concept",take multiple measures to"demand food from rivers,lakes,and seas",and expand aquaculture space.Through research,it has been found that research in the field of facility based fisheries is still in its developmental stage.Further exploration is needed on the current status,breeding models,and experimental demonstrations of land-based facility based fisheries,in order to provide a basis for fisheries regulatory authorities to formulate the"15th Five Year Plan"for facility based fisheries.

The Market Development of Children’s Go Training and the Difficulty and Countermeasure Analysis of Go Training

The children’s Go training market is experiencing vigorous growth but also faces several challenges.As the importance of Go in cultural education increases,the market expands,with growing demand and involvement from training and educational institutions.However,the market encounters difficulties such as a shortage of high-quality Go coaches,uniformity in training content and methods,and high training costs.To address these issues,measures can be taken to strengthen the team of Go coaches,diversify training content and methods,and optimize the training cost structure,thereby promoting the healthy development of the children’s Go training market.

SWOT Analysis and Development Countermeasures of Taohua Mountain Ecotourism Agricultural Park in Ya’an City

By using SWOT Analysis, the internal strengths and weakness, and the external opportunities and challenges of Taohua Mountain Ecotourism Agricultural Park were discussed. The result showed that Taohua Mountain was unique in scenery, rich in rural tourism resources and broad in market prospect. However, the tourism products were monotonous, lack of characteristics and depth; the ecological environment was weak; the tourism staff was with low quality; some parts of the area were in poor sanitary condition, restrained by season and periodicity. Meanwhile, there existed some opportunities and challenges in this park. The opportunities mainly contained policy opportunity and transference of tourists’ preference. The challenges primarily included challenges from urban tourism spots of Ya’an City and surrounding cities, and threats of sustainable use of tourism resources resulting from blind exploitation of fruit production area. And the corresponding development countermeasures were put forward, that is, to develop diversified tourism programs, to allocate mountainous resources according to local conditions, to train the local tourism staff, to dredge the area with poor sanitation, to diversify business with other industries supporting the development of tourism agriculture.

Conflicts and Countermeasures between Haba Snow Mountain Nature Reserve and Development of Peripheral Communities

Using research approaches such as questionnaire investigation, interview, and Participatory Rural Appraisal (PAR), the relationship between Haba Snow Mountain Nature Reserve and peripheral communities was studied. The results showed that there are lots of conflicts between Haba Snow Mountain Nature Reserve and peripheral communities. On the one hand, conservation of natural environment brings inconvenience to the development of peripheral communities, such as limiting the use of resources, constraining space of development, resulting in economic losses, reducing tourist income, and low compensation. On the other hand, peripheral communities' development threats conservation of the natural environment, for instance, illegal deforestation, illegal mining, over-harvesting, over-grazing and non-ecotourism. Therefore, effective suggestions were given as "scientific planning of functional areas, enhancing economic compensation, devoting more in ecotourism development, and realizing the cooperation of communities".

Influence of Urban Development and Construction on Ecological Water Conservancy Landscapes and Countermeasure Analysis——A Case Study of Ecological Water Conservancy Landscapes in Nanchang City,Jiangxi Province of China

Through analyzing influence of urban development and construction on ecological water conservancy landscapes,common ecological problems of existing water conservancy landscapes were explored.Causes responsible for these problems were summarized as poor knowledge about ecology,unclear objective of construction,water conservancy projects violating the principle of conserving and restoring ecological conditions,and degraded biodiversity of water environment.It was stressed that ecological construction of water conservancy landscapes should break traditional water conservancy concepts,adopt ecological treatment and build ecological landscapes;geomorphic,hydrological and hydraulic of original river systems should be maintained as much as possible;sewage not discharged to natural water directly;water circulation enhanced to promote self-purification capacity of water;necessary purification measures adopted for rivers and lakes.On the basis of above strategies,construction of ecological water conservancy landscapes in Nanchang City was taken for example to analyze problems in the previous construction and present achievements,then principles to be followed in the future construction and protection of ecological water conservancy landscapes in Nanchang City were proposed.

Archetype Analysis of Limits-to-growth of Rural Tourism Development in China and Countermeasures

Based on archetype analysis of system dynamics,the limits-to-growth archetype of Peter Senge(one of his eight archetypes) was borrowed,and various influence factors of rural tourism development combined to construct the limits-to-growth archetype which consists of limits-to-growth archetype of scientific planning balance,and limits-to-growth archetype of infrastructure input balance,and limits-to-growth archetype of high-quality staff balance.Through the join of limits-to-growth sub-archetypes,limits-to-growth archetype of rural tourism development in overseas countries were obtained,on the basis of which further researches were carried out to analyze management countermeasures to eliminate limits to growth via rural tourism development.It was stressed that government should play the guiding role,unified planning,scientific management and policy support further enhanced;more efforts should be devoted to the infrastructure construction,and more financial support given by the government;more professional talents should be introduced in multiple ways,and training for present rural tourism staff enhanced.

Research on Climate Change and Countermeasure of Agriculture Sustainable Development

Based on the Fourth Intergovernmental Panel on Climate Change(IPCC) Assessment Report and meteorologists' research results of climate change in China,the influences of climate change on agricultural production were analyzed from the aspects of temperature,precipitation,sea-level rise and so on.Concrete measures were put forward to reply to climate change.According to the climate change trends,defensive measures were established to enhance the capacity of agriculture coping with climate change,making full use of advantages while avoiding disadvantages,in order to achieve sustainable development.

Obstacles and Countermeasures for Policyoriented Agricultural Insurance Development in the Context of Agricultural Modernization

Agricultural insurance is a key impetus for agricultural modernization. How- ever, there are some problems on the aspect, including deficiency of law supports and top system design, less recognition on insurance of farmers and low effective demands of agricultural insurance market, huge agricultural risks and insufficient supply from agricultural insurance market, and shortage of reinsurance support and disaster risk desertification, hardly to deal with heavy disasters. Therefore, some countermeasures were proposed, including to formulate agricultural insurance laws and establish specific agricultural insurance management institutions, to reinforce promotion and improve premium subsidy system in order to increase market de- mands, to increase tax preference and operating costs, improve insurance services and enhance effective supply in market and to construct a risk diversification system of agricultural heavy disaster.

Current Situation and Development Countermeasures of Alpaca Breeding

This paper briefly introduces the biological characteristics and value of alpaca breeding,reviews the development of alpaca breeding,describes the global distribution of alpaca and the current situation of alpaca breeding in the introduction of well-bred,indicates the technology of feeding,managing and deep processing.And finally some countermeasures for future development of alpaca breeding in China are put forward.

Study on Development Countermeasures of Hot-spring Leisure Tourism in Xingzi County of Jiangxi Province

Taking Xingzi hot spring in Jiangxi for an example and tourist behaviors of hot-spring tourism as the research object,tourist behaviors and psychological feelings were analyzed in this study to discuss the sustainable Development Countermeasures of hot-spring tourism.

The influencing factors and Development Countermeasures of Large Farming Household in Jiangxi Province

As an important part of the new agricultural business entities, large-scale grain-production households play a significantly positive role in improving the land resource utilization, improving agricultural productivity, increasing agricultural output and farmers＂ income, and making a certain contribution to stabilize grain production. This paper analyzed the current situation of large-scale grain-production household in Jiangxi Province, as well as the problems in land transfer, farmland infrastructure, production and management, capital and other risks. At last, the paper proposed targeted countermeasures.

Economic Development Status and Regulation Countermeasures of Tourist County in Northeast China Region

Through deep analysis on causes of poor economy in tourist county,the study pointed out that to seek for future development approaches would be the key for economic development of tourist county in Northeast Region.On the basis of identification of connotation,scope and types of tourist county in Northeast Region,economic development status of tourist county in Northeast Region was analyzed by using a large number of statistics,the main existing problems were pointed out and corresponding regulating countermeasures were proposed pertinently.

Problems and Countermeasures of the Bio-energy Development in China

The current development and utilization status of bio-energy at home and abroad was summarized, and a multi-angle in-depth analysis and reflection was given to the problems encountered in the development of bio-energy. Furthermore, some suggestions were put forward to accelerate the further development of bio-en-ergy industry in China.

Research on the Current Status, Paths and Countermeasures of Family Farm Development in China——Investigation and Analysis of Hubei Province

The Chinese family farm is the most important new micro subject in the construction and development of modern agriculture in China. The investigation and analysis of the actual situation of Hubei Province showed that since the implementation of the household contract management system in rural areas, the family farm has followed the track of gradual development, breakthrough development and normal standardization development. In order to promote the family farm to develop rapidly, orderly and healthily, work must be well done to land circulation, scale management, financial support, financial subsidy, cultivation of leading talents and professional farmers, and providing all-round services.

Restraints and Countermeasures for the Sustainable Development of China's Modern Flower Arrangement Art

Factors that influence the development of China's modern flower arrangement art are analyzed in this study,countermeasures and suggestions are proposed for the future development.