A Biogas Production Model from the Combination of Pig Manure and Cow Dung in N’Zérékoré City, Republic of Guine

This present research work focuses on the valorization of pig droppings for production of biogas in mono digestion and co-digestion with proportions of cow dung from the urban commune of N’Zérékoré. It was carried out in December 2020 in the Physics laboratory of the University of N’Zérékoré. The anaerobic digestion process took 25 days in an almost constant ambient temperature of 25˚C. Five digesters were loaded on 12/06/2020, two of which with 1 kg of pig dung and 1 kg of cow dung both in mono-digestion. The 3 other digesters in co-digestion with different proportions of pig manure and cow dung. The substrate in each digester is diluted in 2 liters of water, with a proportion of (1/2). The main results obtained are: 1) the evolution of the temperature and pH during digestion process, 2) the average biogas productions 0.61 liters for (D1);1.20 liter for (D2);1.65 liter for (D3);1.51 liter for (D4) and 1.31 liter for (D5). The cumulative amounts of biogas are respectively: D1 (7.95 liters), D2 (15.60 liters), D3 (21.50 liters), D4 (19.65 liters) and D5 (17.05 liters). The total cumulative production is 81.75 liters at the end of the process. The originality of this research work is that the proposed model examines the relation between the daily biogas production and the variation of temperature, pH and pressure. The combustibility test showed the biogas produced during the first week was no combustible (contains less than 50% methane). Combustion started from the biogas produced from the 15th day and it is from the 20th day that a significant amount of stable yellow/blue flame was observed. The results of this study show the combination of pig manure and cow dung presents advantages for optimal biogas production.

Study of the Optimal Conditions for Anaerobic Digestion of Cow Dung in Households in Cote d’Ivoire

This paper investigates the optimal conditions for methanisation applied to cow dung. Four experimental 25 liters digesters were used in this work. The best biogas yield is obtained when the digester is installed in a metal box and exposed to sunlight. The temperature in this digester varied between 25˚C and 37˚C. The dry matter content of the collected cow dung was 15.5%. The digester was fed with 9 kg of cow dung mixed with 8.5 litres of water, one litre of cassava effluent and 200 ml of human urine. After a retention period of 22 days, the biogas obtained was 67% methane and 21% carbon dioxide. The use of human urine and cassava effluent improved the quality of the biogas.

Performance analysis of cow dung as an eco-friendly additive material for sustainable moulding and casting

In the present work,an attempt is made to partially replace high cost silica sand with sustainable eco-friendly cow-dung.The practical utility of cow dung as an additive material that facilitates binding in sand moulds has been tested.The Taguchi method is used to plan and conduct nine experiments with three replicates each.Pareto analysis of variance study is performed to understand the practical significance of moulding sand variables,that is,percentage of cow dung,clay,water,and degree of ramming on sand mould properties.The conflicting multiple objective functions(maximize mould hardness,and minimize collapsibility and gas evolution)are optimized by utilizing the Data Envelopment Analysis Ranking(DEAR)method.The optimal parameters i.e.6%of clay,4%of water,5%of cow-dung and 4 numbers of ramming strokes are obtained by applying a hybrid Taguchi-DEAR method.These parameters show the best moulding properties are:mould hardness 55,gas evolution 5.9 ml·g-1,and collapsibility 470 g·cm-2.Thereafter,a Lovejoy coupling made of aluminium is cast in the sand mould prepared with cow dung and without cow dung,with the optimum set of parameters.The mechanical properties,surface finish,and microstructure of the casting cast with cow-dung are better than that without cowdung.

Characterization of Digestates from Anaerobic Co-Digestion of Manioc Effluent, Human Urine and Cow Dung

This study focused on the characterization of digestates resulting from anaerobic digestion of manioc effluents from attiéké factories. Two types of digestate were characterized, one consisting of manioc effluent + urine and another composed of manioc effluent + urine + cow dung. As a result, these residues of bio-digestion rich in nutrients (NPK) can be used as agricultural fertilizer. Moreover, the determination of some microorganisms and heavy metals digestates allowed to better appreciate its fertilizing quality. These parameters remained in accordance with the quality standards of a digestate prescribed. These results show that digestates from anaerobic co-digestion of manioc effluents, urine and cow dung can be used without fear as an agricultural biofertilizer.

Development of a Batch-Type Biogas Digester Using a Combination of Cow Dung, Swine Dung and Poultry Dropping

The overdependence on crude oil in African countries warrants the need for alternative sources of energy. A 56-litre-capacity biogas digester was designed, fabricated and evaluated. Investigations were made into the production of biogas from the mixture of cow dung, swine dung and poultry dropping. Standard equations and models were used in the design of the components of the manually operated digester. The digester temperature, ambient temperature and pH were monitored during the experiment. The proximate analysis showed that volatile solid, total solid and moisture content for the mixed substrates at the initial stage were: 64.7%, 83.5% and 13.5% respectively. Similarly, at the digestion stage, the volatile solid, total solid and moisture content were: 54.1%, 22.6% and 74.4% respectively. Advancement of decomposition leads to gradual increasing pH value from 7.2 to 7.4, which indicates the stability of organic matter. The moisture content on a wet basis was initially 13.5%. This value latter increased to 74.4%. On the average, 15 kg of mixed substrates with 25 litres of impure water produced biogas within 25 days of digestion. The gas produced from mixed substrates became flammable with blue flame after 16 days of production, indicating that the ratio of methane gas generated with the three mixtures of fresh animal waste was higher than other gases produced.

Production of Biogas from Co-Digestion of Cow Dung, Saw Dust and Maize Husk

The co-digestion of cow dung, with maize husk for biogas production at laboratory scale was investigated. The study was carried out at a temperature range of??24°C - 30°C and pH range of 5.5 - 6.5 for a period of 60 days with a total solid concentration of 7.4% in the digester sample (fermentation slurry). Water displacement method was used to collect the biogas produced which was subsequently measured. 444.8 mL was the cumulative biogas yield at the end of 60 days retention time in the digester 1, which comprised of cow dung, maize husk, and water. Digester 2, which is made up of sawdust, cow dung, and water produced negligible biogas at the end of 60 days of the experiment. X-RF analysis revealed high presence of elements like silica, aluminium oxides, and aluminium oxides in cow dung, maize husk, and sawdust respectively. The preponderance of alkanes and methyl group inmaize husk makes it to produce biogas compared to saw dust as shown by the Fourier transform infrared spectroscopy (FTIR) that was carried out to identify the various functional groups. The potential of maize husk to produced biogas was also established. The kinetic modeling shows that there was an increase in biogas yield as the retention time increases as depicted by the linear model.

Effects of Enzyme on Material Transformation of Cow Dung Compost

[Objective] The aim was to study change of the composition of cow dung compost by adding complex enzyme.[Method] 2.0% enzyme treatment group,1.5% enzyme treatment group and control group were set to analyze temperature,moisture,pH value,crude fiber,TOC,TN,GI during composting.[Result] The results showed that adding complex enzymes could accelerate degradation of organic matter in pre-composting period.Crude fiber of 2.0%,.1.5% enzyme treatment group and control group decreased 49.6%,47% and 29.1% respectively,TOC decreased 41.7%,35.3% and 21.1%,TN decreased 32.6%,26.8% and 19.2%.2.0%,1.5% enzyme treatment groups could reach basic maturity degree at 30 d.[Conclusion] Composting cycle be shortened by adding complex enzymes,which was useful for maturity of cow dung compost.

K_5CoW_(12)O_(40)·3H_2O催化合成苯甲醛1,2-丙二醇缩醛

研究了K5CoW12O40.3H2O催化剂对苯甲醛和1,2-丙二醇为原料合成苯甲醛1,2-丙二醇缩醛的反应的催化活性。考察了醛醇物质的量比、催化剂质量、带水剂体积、反应时间、催化剂重复使用性等因素对产品收率的影响。结果表明,K5CoW12O40.3H2O是缩醛反应的良好催化剂。在最佳实验条件下:苯甲醛与1,2-丙二醇物质的量比为1:1.2、苯甲醛物质的量为0.1mol、催化剂质量为0.3g、环己烷体积为10mL、反应时间45min,苯甲醛1,2-丙二醇缩醛的收率最高可达92.8%。

赤子爱胜蚓(Eisenia fetida)肠道细菌群落的肠段分异特征及对饥饿-牛粪培养的响应

通过在初始-饥饿-牛粪连续培养条件下采集赤子爱胜蚓(Eisenia fetida)的前肠、中肠和后肠3个肠段内容物进行细菌16S rRNA基因测序分析,研究蚯蚓肠道细菌群落的肠段分异特征及对饥饿和食物培养的响应。结果显示:细菌群落多样性和组成在相邻肠段间相似,中肠和后肠的相似程度高于前肠和中肠,在相隔肠段间差异较大。厚壁菌门(14%~55%)、放线菌门(16%~39%)和变形菌门(15%~37%)是所有蚯蚓肠段的主要优势菌群。在不同培养状态下的肠道传递(前肠-中肠-后肠)过程中,优势菌门丰度变化趋势不同。前肠、中肠和后肠的核心扩增序列变体(ASVs)主要属于厚壁菌门(35%~48%)、放线菌门(20%~26%)和γ变形菌纲(14%~16%),前肠和后肠具有独有的核心ASVs,分别属于δ变形菌纲(7%)和纤维杆菌门(6%)。从初始状态到饥饿状态,肠道菌群Shannon多样性显著降低(P<0.05),Sobs丰富度降低,但未达显著水平(P>0.05);肠道中α/δ变形菌纲和放线菌门等类群相对丰度显著降低(P<0.05),γ变形菌纲、厚壁菌门和拟杆菌门相对丰度显著提高(P<0.05)。从饥饿状态到牛粪培养状态,肠道菌群Shannon多样性和Sobs丰富度进一步显著降低(P<0.05);肠道中δ变形菌纲和厚壁菌门等类群相对丰度显著提高(P<0.05),γ/α变形菌纲和放线菌门等类群相对丰度显著降低(P<0.05),拟杆菌门相对丰度无显著变化(P>0.05)。聚类分析结果表明,蚯蚓肠道菌群具有一定的稳定性。结合菌群结构和差异ASVs分析结果表明,饥饿-牛粪处理显著改变了蚯蚓肠道细菌群落,其中,饥饿处理主要改变菌群丰度,而牛粪处理同时改变菌群丰度和组成。综上所述,蚯蚓肠道微生物群落存在明显的肠段分异,且在一定程度上具有抵抗干扰以保持稳态的能力。蚯蚓肠道微生物群落对饥饿和食物均有明显响应,其中对食物的响应程度更强。

Microbial safety control of compost material with cow dung by heat treatment

Various kinds of pathogenic bacteria derived from the intestinal tract of animals exist in compost material like cow dung. In order to sterilize the pathogenic bacteria completely in compost material, the cow dung was put into a heat treatment machine in pilot plan, and harmless condition in short time was examined. The results indicated, pathogenic indicator bacteria such as coliform bacteria, fecal coliform, Escherichia coli and salmonella were all 106 cfu/g dw at the beginning, died rapidly when cow dung temperature rose to above 50~C, and not detected at 54-68~C for 6-24 h heat treatment. Coliform bacteria and salmonella in heated cow dung were not detected by re-growth culture and enrichment culture examination. Moreover, it was hardly influenced on the fermentation ability of composting microbe, organic decomposition bacteria. During heat treatment, the mesophile decreased rapidly and the thermophile stabilized or increased, and the most of composting microbe were bacillus in cow dung by fluorescence microscope, this indicated that bacillus was dominator and composting microbe in composting process.

Detection of tyrosine,trace metals and nutrients in cow dung:the environmental significance in soil and water environments

This study examined the dissolved organic matter(DOM) components of cow dung using a combination of fluorescence(excitation–emission matrix,EEM)spectroscopy and parallel factor(PARAFAC) modelling along with eleven trace metals using ICP-MS and nutrients(NH_4^+ and NO_3^-) using an AA3 auto analyser. EEM–PARAFAC analysis demonstrated that cow dung predominantly contained only one fluorescent DOM component with two fluorescence peaks(Ex/Em=275/311 nm and Ex/Em=220/311 nm),which could be denoted as tyrosine by comparison with its standard. Occurrence of tyrosine can be further confirmed by the FTIR spectra. Trace metals analysis revealed that Na,K and Mg were significantly higher than Ca,Fe,Mn,Zn Sr,Cu,Ni and Co. The NH_4^+ concentrations were substantially higher than NO_3^-.These results thus indicate that the dissolved components of the cow dung could be useful for better understanding its future uses in various important purposes.

Assessment on Potential of Cow Dung Manure in <i>Zea mays</i>Production at Kiwere Village in Iringa Rural District, Tanzania

An assessment on potentiality of Cow dung manure in Zea mays production at Kiwere village in Iringa Rural District (7°37'17.3"S, 35°37'48.1"E) was conducted from December 2019 to June 2020. The idea of this assessment was influenced by soil fertility depletion and the fall of Maize production in Iringa region from 1990’s. A total of 10 farms, 5 farms for Cow dung manured and 5 for non-manured in which Maize growth was monitored for 6 months. Various parameters as height, width and weight of both Maize plants and Maize fruits were recorded. Soil qualities both chemical and physical characteristics were analysed and recorded but also weather (Temperature, humidity) together with soil pH were recorded throughout the assessment period. The p-value of <0.0001 (1.03702E-07) and <0.0001 (2.45684E-05) for height and width and the highest mineral chemical values in the Cow manured 10 plots indicated that Cow manure not only increase Maize production significantly but also supports the production of soil microbial and hence renewals the soil minerals and soil fertility as well. This is why the soil physical values in the Cow dung manured plots Organic matter, porosity, soil aeration and others were found to be higher and the soil comprised of Sandy Clay Loam. Farmers are strongly advised to use this multipurpose and potential manure in their unfertile soil. Cow manures are easily available in their environment and cheaper. -

Amelioration of Saline Soil by the Application of Gypsum, Calcium Chloride, Rice Husk and Cow Dung

A pot experiment was conducted to investigate the effect of cow dung, rice husks, calcium chloride and gypsum on soil reclamation and compare the effect of organic and inorganic amendments on soil reclamation during the period of 5th March to 20th April, 2017. The experiment was laid to fit a completely randomized design (CRD) with seven treatments [Reference soil (T0), Cow dung (T1), Rice husk (T2), Gypsum (T3), Calcium chloride (T4), Cow dung + Rice husk (T5) and Gypsum + Calcium chloride (T6)] each having three replications for this experiment. After incubation (45 days), the laboratory investigation was carried out in the Soil, Water and Environment Discipline, Khulna University, Khulna, Bangladesh. Results indicate that the individual or combined effect of gypsum (T3) was more effective in changing EC and SAR. Gypsum application in combination with calcium chloride (T6) improved the soil chemical properties by reducing the EC. Among the treatment, calcium chloride (T4) had a remarkable effect in reducing sodium adsorption ratio and gypsum had a remarkable effect in reducing pH. Cow dung (T1), rice husk (T2), combination of cow dung and rice husk (T5) were less effective to reduce EC, pH and SAR. It’s measured for soils of different soil amendments varied significantly

Organic Amendments with Poultry Manure and Cow Dung Influence the Yield and Status of Nutrient Uptake in Wheat (Triticum aestivum)

The field experiment cultivating wheat during the winter season from November 2014 to March 2015 was conducted in the “North Eastern Barind Tract Soils” at Kushadaha, Nawabganj, Dinajpur in Bangladesh. The wheat variety BARI Gom 26 was selected in focusing to evaluate the effects of organic amendments using poultry manure (PM) and cow dung (CD) on the growth, yield and nutrient uptake by the plant. The texture of the field soil was clay with acidic nature (pH 5.61). The trials comprise three treatments following a randomized complete block design (RCBD) replicated thrice. Results showed that significantly greater plant height, number of tillers per hill and straw yield were 98.10 cm, 3.66 and 5425 kg/ha respectively in receiving the treatment T2 than T3 (95.66 cm, 3.38 and 4483 kg/ha) and T1 (control). Spike length, 1000 grain weight and grain yield were 9.23 cm, 39.81 kg/ha and 3100 kg/ha respectively also higher in T2 treatment than T3 (8.76 cm, 38.51 kg/ha, 3091 kg/ha) with no statistical differences among them. Therefore, the treatment T2 (PM) comprised of poultry manure and NPK exhibited as the best treatment for producing the highest in all growth and yield parameters of wheat. In wheat grain, the content of N, P, K, S, Mg, Zn and B ranged from 1.080% to 1.380% N, 0.390% to 0.398% P, 0.780% to 0.840% K, 0.079% to 0.111% S, 0.181% to 0.187% Mg, 25.56 to 29.77 ppm Zn and 10.12 to 12.54 ppm B. Similarly in straw, nutrients content ranged from 0.220 to 0.300% N, 0.045% to 0.074% P, 0.970% to 1.250% K, 0.152% to 0.191% S, 0.097% to 0.101% Mg, 10.78 to 13.23 ppm Zn and 27.98 to 2989 ppm B. Therefore, organic amendment with 5-ton cow dung (CD) ha-1 with a recommended dose of NPK significantly increased the content of N, S, Mg, Zn and B in the grain and straw of wheat. Alternatively, the poultry manure treatment with NPK significantly increased the content of P and K in the grain and straw of wheat. However, results revealed that the treatments T2 and T3 comprising a recommended dose of NPK with poultry manure and cow dung respectively could increase the content of N, P, K, S, Mg, Zn and B in wheat. The overall results expressed that the poultry manure treatment with NPK (T2) exposed as superior for producing the top growth and yield attributes of wheat in the studied area.

Experimental Study of Biogas Production from Cow Dung as an Alternative for Fossil Fuels

To treat the problem of fossil fuel usage and greenhouse gas emissions, biogas is considered a potential source of clean renewable energy. The aim of the work is to analyze the amount of biogas and ph from cow dung when an anaerobic digester operates in the mesophilic mode. In this study is presented the experimental investigation of biogas production from cow dung as an alternative energy resource. This is work using an 18 Liters capacity plastic as prototype biogas plant, plant to inspect the anaerobic digestion in producing biogas. The digester was batch operated and daily gas produced from the plant was observed for 30 days. The digester was fed within the ratio of 1:1 of dung to water respectively. The operating temperatures of the digester were maintained within mesophilic conditions. The Biogas production from cow dung fluctuates from the first day to the thirtieth day between 0 and 340 ml. The pH of cow dung is gradual reduction within the retention period.

Influence of Climate Temperature on the Valorization of Dung-Wastewater Slaughterhouse Biogas in Two Regions: In Chad and Senegal

In this work, we have produced biogas by co-digestion of cow dung and slaughterhouse wastewater under different climate temperatures in two countries: N’Djamena in Chad, a country of Central Africa and Dakar in Senegal, a country of West Africa. In a first approach, we put the cow dung in cans of 1.5 L, hermetically closed. The goal was to know how long you could produce biogas. Then we built a bio-digester to produce biogas for cooking. Each bio-digester was exposed to receive solar heat that varied between 27°C to 41°C in Chad and between 24°C to 30°C in Senegal. Influenced by the high temperature and a minimum residence time, the experiments showed that the N’Djamena test produced biogas more quickly than the Dakar test which, on the contrary, had a low temperature and a long residence time. The production of biogas began at the end of seven days with flammability on the twenty-first day for the bio-digester in Chad and after twenty-seven days with a flammability on the thirty-sixth day for the bio-digester of Senegal. The different digestates were valorised in fertilizers, bricks and green coal. Our research aims to meet the living conditions of the rural world specifically for women by reducing their work and thus allowing them to have more time to self-educate and educate their children.

Comparative Effect of Foliar Application of Cow Dung, Wood Ash and Benlate on the Disease Initiation and Development of Roselle (Hibiscus sabdariffa L.) Leaf Spot Disease Caused by Coniella musaiensis Var. Hibisci. in Makurdi, Central Nigeria

A study on the comparative effect of cow dung, wood ash and benlate for the control of leaf spot disease of Roselle (Hibiscus sabdariffa L.) was carried out following the preparation and foliar application of the materials on selected Roselle plants of the green and red accessions (Acc1 & Acc3) in the field. The cow dung was fermented for 14 days while sour milk was added to wood ash, dissolved and all filtered and labeled. The spraying commenced soon after the plants were thinned to one and was done weekly until after 50% of the plants had flowered. Results showed significant (p = 0.05) difference between benlate, the synthetic fungicide and the bio-fertilizers (cow dung and wood ash) for the two years of the study. Disease severity was significantly lower in the benlate than in the bio-fertilizers. There was no significant difference in yield between the treatments although higher yield was recorded in benlate. Though the bio-fertilizers could not suppress infection, they aided the growth of the plants by boosting luxuriant growth. The result showed that these bio-fertilizers cannot be used as substitute to the synthetic fungicide for the control of this leaf spot disease caused by Coniella musaiensis var. hibisci. However, since they give equally good yield the bio-fertilizers can still be used to minimize the reliance upon use of chemical fungicides.

Inhibitory Activities of Sole Cow Urine and Combined Cow Dung/Cow Urine against the Blight Disease of Ribwort (<i>Plantago lanceolata</i>) at the Cistercian Monastery in Mbengwi, Cameroon

Ribwort (Plantago lanceolata) is a small glabrous to pubescent perennial plant that is native to Europe, America, North Africa and Asia. Nowadays, it is cultivated in many countries across the globe, including Cameroon due to its extensive use in livestock and medicine. Unfortunately in Cameroon, however, the plant has been highly infested by blight, reducing its yield and medicinal value. To reduce blight infestation of ribwort and improve plant yields, we aimed to compare the efficacy of sole cow urine and combine cow dung/cow urine to inhibit blight disease caused by Phyllosticta ophiopogonis on ribwort. At the Cistercian Monastery in Mbengwi, Momo Division, Cameroon, we used a Randomized Complete Block Design (RCBD) with 3 Blocks consisting of two treatments (cow dung mixed with cow urine (combine cow dung/urine) and sole cow urine) and one control. After spraying the different blocks of ribworts plants with combined cow dung/urine and sole cow urine at a dosage of 3% concentration, we found an incidence of blight disease of 32.8% and 35.0% on ribworts sprayed with combined cow dung/urine and sole cow urine, respectively, compared to 67.8% in the control. This implies that a mixture of cow dung/cow urine reduces the incidence of blight disease significantly. Furthermore, our pathogenicity test showed that Phyllosticta ophiopogonis (fungus) was responsible for the blight disease. Therefore, to increase ribwort growth, improve adaption and reduce Phyllosticta ophiopogonis fungal infestation in Cameroon, we recommend that the plant should be sprayed with a mixture of cow dung and cow urine at 3% concentration.

Development of an Anaerobic Digestion Unit for Biogas Production from Cow Dung Substrate

An anaerobic digestion unit for producing biogas from cow dung in the rural communities was designed, fabricated and tested for performance, durability and throughput. The major components of the digester included the substrate holding tank, tank cover, agitator, debris collector, inlet and outlet pipes, gas reception tank, hose and heat source. The digester is a vertical cylindrical tank with an inlet pipe for the introduction of substrate and an outlet pipe to collect the digested substrate. An agitator is incorporated inside the digester to break scum on the substrate and create uniform temperature profile in the digester while a pressure gauge was fitted to the gas outlet valve to measure the gas pressure in the tank. The agitator shaft is extended outside to be driven by an electric motor through belt and pulley system. The criteria considered in the design of the digester included air tightness of the system, mesophilic and thermophilic temperature, nature and type of substrate used, substrate retention period, number of crank turns per minute and volumetric capacity of the digestion tank. Other considerations included the desire to make the digestion tank and gas reception tank of galvanized steel to ensure good quality of the product and the need for a strong structural support to ensure structural stability of the system. After construction and assembly, the biogas digestion unit was tested with 40 kg of cow dung diluted with 80 kg of water and subjected to a retention period to make a substrate （slurry） of 10 % total solid （TS）. Daily gas yield was determined; gas pressure in the tank was measured by the pressure gauge, while the ambient temperature was taken at five hours interval. Results showed that a cumulative gas yield of 0.415 litres after 22 d retention period at average substrate temperature and pH of 29 ℃ and 6.2, respectively. The digester has a substrate holding capacity of 330.8 litres and a production cost of $375 with all the construction materials being available locally.

Design Biogas Production from Mixed Napier Pak Chong I/Food Waste at Thermophilic Temperature by Anaerobic Digestion in Cow Dung and Chicken Dung

AD （anaerobic digestion） is a beneficial and efficient technique for the treatment of agricultural wastes, food wastes and wastes water to produce renewable energy. Solid agricultural are potential renewable energy resoures. Biogas production by co-digestion of mixed Napier Pak Chong I and food waste at thermophilic temperature using anaerobic digestion in cow dung and chicken dung as the seed inoculums were investigated. The total reactor volume of the co-digester reactor was 7.94 m^3, which was equipped with pump, and it was operated continuously for the 20 days as a pilot scale at 50 ℃. The Napier Pak Chong I was cut into 2 mm sections, and the initial VS （volatile solids） was 30%. The initial VS of food waste were 70%. Two pilot-scale digesters filled with Napier Pak Chong I and food waste, which both digesters contained 476 kg of Napier Pak Chong I mixed 305 L of food waste, and 1305 L of water. There were carried out to investigate the optimum C/N （carbon to nitrogen） ratio for effective biogas production. The slurry raw materials provided sufficient buffering capacity to maintain appropriate pH values （between 7.0 and 8.0）. Digester I was designed for 1.98 m^3 of cow dung as the seed inoculum while digester II was designed to establish 1.98 m^3 of chicken dung as the seed inoculum. Gas detector performs analysis gas production. The m^3/day in digester I and 1.86 m^3/day from digester II, resulting in added, respectively. Biogas production in digester I was directly experimental results indicate that total biogas production was 2.19 specific methane yields of 1.26 m^3 CH4/kgVS and 1.07 m^3 CH4/kgVS correlated with temperature.