High resistance thin film chip resistors(0603 type) were studied,and the specifications are as follows:1 k? with tolerance about ±0.1% after laser trimming and temperature coefficient of resistance(TCR) less than...High resistance thin film chip resistors(0603 type) were studied,and the specifications are as follows:1 k? with tolerance about ±0.1% after laser trimming and temperature coefficient of resistance(TCR) less than ±15×10-6/℃.Cr-Si-Ta-Al films were prepared with Ar flow rate and sputtering power fixed at 20 standard-state cubic centimeter per minute(sccm) and 100 W,respectively.The experiment shows that the electrical properties of Cr-SiTa-Al deposition films can meet the specification requirements of 0603 ty...展开更多
High power pulsed magnetron sputtering（HPPMS）, a novel physical vapor deposition technology, was applied to prepare vanadium films on aluminum alloy substrate in this paper. The influence of target–substrate dista...High power pulsed magnetron sputtering（HPPMS）, a novel physical vapor deposition technology, was applied to prepare vanadium films on aluminum alloy substrate in this paper. The influence of target–substrate distance（Dt–s）（ranging from 8 to 20 cm） on phase structure, surface morphology, deposition rate, and corrosion resistance of vanadium films was investigated. The results show that the vanadium films are textured with a preferential orientation in the（111） direction except for that fabricated at 20 cm. With Dt–sincreasing, the intensity of（111） diffraction peak of the films decreases and there exists a proper distance leading to the minimum surface roughness of 0.65 nm. The deposition rate decreases with Dt–sincreasing. All the V-coated aluminum samples possess better corrosion resistance than the control sample. The sample fabricated at Dt–sof 12 cm demonstrates the best corrosion resistance with the corrosion potential increasing by 0.19 V and the corrosion current decreasing by an order of magnitude compared with that of the substrate. The samples gain further improvement in corrosion resistance after annealing, and if compared with that of annealed aluminum alloy, then the corrosion potential of the sample fabricated at 20 cm increases by 0.415 V and the corrosion current decreases by two orders of magnitude after annealed at 200 °C. If the annealing temperature further rises to 300 °C, then the corrosion resistance of samples increases less obviously than that of the control sample.展开更多
目的:观察高迁移率族蛋白1(high-mobility group box 1,HMGB1)对急性髓系白血病细胞株(human leukemiacell line,K562)自噬及化学治疗(化疗)耐药的影响,并探讨其相关的分子机制。方法:体外培养K562细胞,分为化疗药物处理组、化疗药物处...目的:观察高迁移率族蛋白1(high-mobility group box 1,HMGB1)对急性髓系白血病细胞株(human leukemiacell line,K562)自噬及化学治疗(化疗)耐药的影响,并探讨其相关的分子机制。方法:体外培养K562细胞,分为化疗药物处理组、化疗药物处理对照组、HMGB1纯化蛋白预处理组、HMGB1纯化蛋白预处理对照组、HMGB1si RNA转染组和HMGB1 si RNA转染对照组,其中化疗药物处理组又分为长春新碱(v incristine,VCR)、足叶乙甙(etoposide,VP-16)、阿糖胞苷(cytosine arabinoside,Ara-C)、阿霉素(adriamycin,ADM)和三氧化二砷(arsenic trioxide,As2O3)处理组。细胞计数试剂盒-8检测细胞活性;Western印迹检测HMGB1,微管相关蛋白1轻链3(microtubule-associate protein1light chain3,LC3),腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)和哺乳动物雷帕霉素靶点(mammalian target of rapamycin,m-TOR)磷酸化蛋白表达水平;ELISA法检测细胞上清液HMGB1蛋白含量;单丹磺酰尸胺染色和透射电镜观察细胞自噬状态。结果:与相应对照组比较,各化疗药物处理组(VCR,VP-16,Ara-C,ADM和As2O3)的细胞活性均显著降低,HMGB1蛋白表达均显著上调(均P<0.05)。与相应对照组比较,HMGB1纯化蛋白预处理组的细胞活性显著增加(P<0.05),HMGB1蛋白表达显著上调(P<0.05)。与HMGB si RNA转染对照组比较,HMGB1si RNA转染组的细胞活性显著降低(P<0.05),HMGB1蛋白表达显著下调(P<0.05);与相应对照组比较,HMGB1纯化蛋白处理组中LC3-II表达明显增强(P<0.05),光镜下观察到自噬小体和自噬泡数量明显增加。同时,与相应对照组比较,HMGB1纯化蛋白处理组p-AMPKa的蛋白表达明显增强,而p-m TOR表达明显减弱(均P<0.05)。结论:HMGB1可能通过AMPK/m-TOR信号通路增强K562细胞自噬发挥化疗耐药性。展开更多
目的:研究具有肿瘤多药耐药逆转活性的四氢异喹啉类化合物HZ08-重组高密度脂蛋白(reconsdtuted high density lipoprotein,rHDL)纳米粒的构建及性质,旨在研制HZ08的新型仿生纳米制剂。方法:通过胆酸钠法制备HZ08-rHDL纳米粒,考察该纳米...目的:研究具有肿瘤多药耐药逆转活性的四氢异喹啉类化合物HZ08-重组高密度脂蛋白(reconsdtuted high density lipoprotein,rHDL)纳米粒的构建及性质,旨在研制HZ08的新型仿生纳米制剂。方法:通过胆酸钠法制备HZ08-rHDL纳米粒,考察该纳米粒的包封率、载药量、渗漏率、形态、粒径等理化性质,透析法研究制剂的体外释放特性,MTT法考察rHDL载体对人乳腺癌细胞(MCF-7)和人正常乳腺细胞(MCF-10A)的毒性。HPLC法、流式细胞术和荧光显微术评价纳米粒的肿瘤细胞靶向性。结果:HZ08-rHDL纳米粒包封率(93.45±0.28)%,载药量(10.65±0.46)%,水分散液于4℃放置一个月渗漏率为(4.50±0.12)%,外观呈圆整球形,平均粒径为(105.53±2.50)nm;体外48 h累积释放量仅为(12.13±1.08)%;rHDL载体细胞毒性低,且MCF-7细胞对rHDL荷栽药物的摄取能力显著强于MCF-10A细胞(P<0.0001),有较强的体外肿瘤靶向性。结论:HZ08-rHDL纳米粒的包封率高、性质稳定、粒径大小适宜、缓释效果明显、载体毒性低且有较好的肿瘤细胞靶向性,具有继续研究开发的价值。展开更多
基金Supported by Science and Technology Committee of Tianjin (No.06YFGPGX08400)Ministry of Science and Technology of China (No.2009GJF20022)Innovation Fund of Tianjin University
文摘High resistance thin film chip resistors(0603 type) were studied,and the specifications are as follows:1 k? with tolerance about ±0.1% after laser trimming and temperature coefficient of resistance(TCR) less than ±15×10-6/℃.Cr-Si-Ta-Al films were prepared with Ar flow rate and sputtering power fixed at 20 standard-state cubic centimeter per minute(sccm) and 100 W,respectively.The experiment shows that the electrical properties of Cr-SiTa-Al deposition films can meet the specification requirements of 0603 ty...
基金financially supported by the National Natural Science Foundation of China (Nos. 51175118 and U1330110)the Open Foundation of Science and Technology on Surface Physics and Chemistry Laboratory (No. SPC201104)
文摘High power pulsed magnetron sputtering（HPPMS）, a novel physical vapor deposition technology, was applied to prepare vanadium films on aluminum alloy substrate in this paper. The influence of target–substrate distance（Dt–s）（ranging from 8 to 20 cm） on phase structure, surface morphology, deposition rate, and corrosion resistance of vanadium films was investigated. The results show that the vanadium films are textured with a preferential orientation in the（111） direction except for that fabricated at 20 cm. With Dt–sincreasing, the intensity of（111） diffraction peak of the films decreases and there exists a proper distance leading to the minimum surface roughness of 0.65 nm. The deposition rate decreases with Dt–sincreasing. All the V-coated aluminum samples possess better corrosion resistance than the control sample. The sample fabricated at Dt–sof 12 cm demonstrates the best corrosion resistance with the corrosion potential increasing by 0.19 V and the corrosion current decreasing by an order of magnitude compared with that of the substrate. The samples gain further improvement in corrosion resistance after annealing, and if compared with that of annealed aluminum alloy, then the corrosion potential of the sample fabricated at 20 cm increases by 0.415 V and the corrosion current decreases by two orders of magnitude after annealed at 200 °C. If the annealing temperature further rises to 300 °C, then the corrosion resistance of samples increases less obviously than that of the control sample.
文摘目的:观察高迁移率族蛋白1(high-mobility group box 1,HMGB1)对急性髓系白血病细胞株(human leukemiacell line,K562)自噬及化学治疗(化疗)耐药的影响,并探讨其相关的分子机制。方法:体外培养K562细胞,分为化疗药物处理组、化疗药物处理对照组、HMGB1纯化蛋白预处理组、HMGB1纯化蛋白预处理对照组、HMGB1si RNA转染组和HMGB1 si RNA转染对照组,其中化疗药物处理组又分为长春新碱(v incristine,VCR)、足叶乙甙(etoposide,VP-16)、阿糖胞苷(cytosine arabinoside,Ara-C)、阿霉素(adriamycin,ADM)和三氧化二砷(arsenic trioxide,As2O3)处理组。细胞计数试剂盒-8检测细胞活性;Western印迹检测HMGB1,微管相关蛋白1轻链3(microtubule-associate protein1light chain3,LC3),腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)和哺乳动物雷帕霉素靶点(mammalian target of rapamycin,m-TOR)磷酸化蛋白表达水平;ELISA法检测细胞上清液HMGB1蛋白含量;单丹磺酰尸胺染色和透射电镜观察细胞自噬状态。结果:与相应对照组比较,各化疗药物处理组(VCR,VP-16,Ara-C,ADM和As2O3)的细胞活性均显著降低,HMGB1蛋白表达均显著上调(均P<0.05)。与相应对照组比较,HMGB1纯化蛋白预处理组的细胞活性显著增加(P<0.05),HMGB1蛋白表达显著上调(P<0.05)。与HMGB si RNA转染对照组比较,HMGB1si RNA转染组的细胞活性显著降低(P<0.05),HMGB1蛋白表达显著下调(P<0.05);与相应对照组比较,HMGB1纯化蛋白处理组中LC3-II表达明显增强(P<0.05),光镜下观察到自噬小体和自噬泡数量明显增加。同时,与相应对照组比较,HMGB1纯化蛋白处理组p-AMPKa的蛋白表达明显增强,而p-m TOR表达明显减弱(均P<0.05)。结论:HMGB1可能通过AMPK/m-TOR信号通路增强K562细胞自噬发挥化疗耐药性。
文摘目的:研究具有肿瘤多药耐药逆转活性的四氢异喹啉类化合物HZ08-重组高密度脂蛋白(reconsdtuted high density lipoprotein,rHDL)纳米粒的构建及性质,旨在研制HZ08的新型仿生纳米制剂。方法:通过胆酸钠法制备HZ08-rHDL纳米粒,考察该纳米粒的包封率、载药量、渗漏率、形态、粒径等理化性质,透析法研究制剂的体外释放特性,MTT法考察rHDL载体对人乳腺癌细胞(MCF-7)和人正常乳腺细胞(MCF-10A)的毒性。HPLC法、流式细胞术和荧光显微术评价纳米粒的肿瘤细胞靶向性。结果:HZ08-rHDL纳米粒包封率(93.45±0.28)%,载药量(10.65±0.46)%,水分散液于4℃放置一个月渗漏率为(4.50±0.12)%,外观呈圆整球形,平均粒径为(105.53±2.50)nm;体外48 h累积释放量仅为(12.13±1.08)%;rHDL载体细胞毒性低,且MCF-7细胞对rHDL荷栽药物的摄取能力显著强于MCF-10A细胞(P<0.0001),有较强的体外肿瘤靶向性。结论:HZ08-rHDL纳米粒的包封率高、性质稳定、粒径大小适宜、缓释效果明显、载体毒性低且有较好的肿瘤细胞靶向性,具有继续研究开发的价值。
