The cultivation of genetically modified （GM） plants requires the reduction of an unwanted spread of genes （biological confinement）. Cytoplasmic male sterility （CMS） inhibits the development of functional pollen,...The cultivation of genetically modified （GM） plants requires the reduction of an unwanted spread of genes （biological confinement）. Cytoplasmic male sterility （CMS） inhibits the development of functional pollen, but nuclear restorer （RJ） genes and environmental impacts can restore the fertility. The aim of this study was to verify whether CMS in maize hybrids is a reliable confinement method for the prospective cultivation of GM maize in Germany. Two-year field experiments in three different environments were conducted with three CMS maize hybrids which vary in the CMS stability, one conventional maize variety （all yellow kernels） and white maize as pollen recipient. Tassel characteristics, pollen vitality and cross-pollination rates were investigated. The CMS stability was dependent on the genotype and the specific weather conditions per year and location. In all maize hybrids CMS was unstable. One CMS maize hybrid showed a high level of CMS stability and very low cross-pollination rates in any case （〈 1%）. The two other CMS maize hybrids developed more fluctuant and fertile tassels with few or many pollen, respectively. Compared with a conventional and fully fertile maize variety, cross-pollination of all CMS maize hybrids was strongly reduced （84%-99%）. In conclusion, the CMS trait can be proposed as a useful biological confinement method to reduce pollen-mediated gene flow from GM maize.展开更多
A field experiment was conducted in Taiwan to measure the cross-pollination (CP) rate of maize pollen recipients from pollen sources using phenotypic marker and to determine the isolation dis- tance between the 2 maiz...A field experiment was conducted in Taiwan to measure the cross-pollination (CP) rate of maize pollen recipients from pollen sources using phenotypic marker and to determine the isolation dis- tance between the 2 maize varieties. A waxy variety (Black Pearl) with purple kernels simulated the genetically modified (GM) pollen donor, and another waxy variety (White Pearl) with white kernels simulated the non-GM recipient. For the first crop, the total area was approximately1.5 hawith a pollen source and recipient acreage ratio of approximately 1:32. For the second crop, the total area was approximately1.83 hawith a ratio of approximately 1:17.3. The source fields were surrounded by the recipient fields for 2 crop seasons. The results showed that the rate of CP was <0.05% beyond15 mupwind and84.8 mdownwind in all crop seasons. The CP rate was below 5% at a distance of10min the downwind direction. A sample with 0.24% CP was recorded at107.3 mdownwind;however, the CP rate was 0% at68 mupwind. Three empirical models were used, that is, exponential, log/log and log/log, and a simplified Gaussian Plume model, to examine the relationship between the CP rates and the source-field distances. All of the models were appropriate for predicting CP rates, and the Gaussian Plume model performed better compared to the empirical models. The results show that it is possible to control CP from foreign pollen by using an appropriate isolation distance.展开更多
In megacities, the phenomenon of the highrise buildings which add fashion elements into the body and facades seems as big sculptures. Architect composed their work by applying the cross-pollination of design method. T...In megacities, the phenomenon of the highrise buildings which add fashion elements into the body and facades seems as big sculptures. Architect composed their work by applying the cross-pollination of design method. They adopted the various elements to enrich their building＇s facade in order to look different. On the other hand, the traditional architecture expressed the tribe civilization by placing their proud artifacts into the building＇s facade. The purpose of this study is to find a new theory of architecture during their process of becoming of the architecture＇s form. The study applied a phenomenological investigation method of several highrise building＇s facade at Jakarta and several districts of Singapore, Brisbane and Melbourne. The study result is a new theory named ＂the fashion-architecture＂ is a building composed by adopting the cross-pollination of design method. To enrich the building＇s facade, architect adopted anything including the fashion＇s design elements： accessories, millineries, and cosmetics also reflected by the traditional architecture. Their fashion-architecture works are reflecting the tribe civilization into a contemporary architecture design.展开更多
文摘The cultivation of genetically modified （GM） plants requires the reduction of an unwanted spread of genes （biological confinement）. Cytoplasmic male sterility （CMS） inhibits the development of functional pollen, but nuclear restorer （RJ） genes and environmental impacts can restore the fertility. The aim of this study was to verify whether CMS in maize hybrids is a reliable confinement method for the prospective cultivation of GM maize in Germany. Two-year field experiments in three different environments were conducted with three CMS maize hybrids which vary in the CMS stability, one conventional maize variety （all yellow kernels） and white maize as pollen recipient. Tassel characteristics, pollen vitality and cross-pollination rates were investigated. The CMS stability was dependent on the genotype and the specific weather conditions per year and location. In all maize hybrids CMS was unstable. One CMS maize hybrid showed a high level of CMS stability and very low cross-pollination rates in any case （〈 1%）. The two other CMS maize hybrids developed more fluctuant and fertile tassels with few or many pollen, respectively. Compared with a conventional and fully fertile maize variety, cross-pollination of all CMS maize hybrids was strongly reduced （84%-99%）. In conclusion, the CMS trait can be proposed as a useful biological confinement method to reduce pollen-mediated gene flow from GM maize.
文摘A field experiment was conducted in Taiwan to measure the cross-pollination (CP) rate of maize pollen recipients from pollen sources using phenotypic marker and to determine the isolation dis- tance between the 2 maize varieties. A waxy variety (Black Pearl) with purple kernels simulated the genetically modified (GM) pollen donor, and another waxy variety (White Pearl) with white kernels simulated the non-GM recipient. For the first crop, the total area was approximately1.5 hawith a pollen source and recipient acreage ratio of approximately 1:32. For the second crop, the total area was approximately1.83 hawith a ratio of approximately 1:17.3. The source fields were surrounded by the recipient fields for 2 crop seasons. The results showed that the rate of CP was <0.05% beyond15 mupwind and84.8 mdownwind in all crop seasons. The CP rate was below 5% at a distance of10min the downwind direction. A sample with 0.24% CP was recorded at107.3 mdownwind;however, the CP rate was 0% at68 mupwind. Three empirical models were used, that is, exponential, log/log and log/log, and a simplified Gaussian Plume model, to examine the relationship between the CP rates and the source-field distances. All of the models were appropriate for predicting CP rates, and the Gaussian Plume model performed better compared to the empirical models. The results show that it is possible to control CP from foreign pollen by using an appropriate isolation distance.
文摘In megacities, the phenomenon of the highrise buildings which add fashion elements into the body and facades seems as big sculptures. Architect composed their work by applying the cross-pollination of design method. They adopted the various elements to enrich their building＇s facade in order to look different. On the other hand, the traditional architecture expressed the tribe civilization by placing their proud artifacts into the building＇s facade. The purpose of this study is to find a new theory of architecture during their process of becoming of the architecture＇s form. The study applied a phenomenological investigation method of several highrise building＇s facade at Jakarta and several districts of Singapore, Brisbane and Melbourne. The study result is a new theory named ＂the fashion-architecture＂ is a building composed by adopting the cross-pollination of design method. To enrich the building＇s facade, architect adopted anything including the fashion＇s design elements： accessories, millineries, and cosmetics also reflected by the traditional architecture. Their fashion-architecture works are reflecting the tribe civilization into a contemporary architecture design.
