A method for Improving the Efficiency of Pear Tree Crossbreeding

In view of the short blooming period of pear tree crossbreeding and the complexity of pollination process, a method that can improve the efficiency of crossbreeding of pear trees was provided. Meanwhile, this method can also be applied to the study of pollen xenia effect, pollination tree selection and pure pollen collection in pear tree cultivation.

Research advances in genetics and breeding of Populus davidiana Dode in China

In this paper a general introduction is given to research advances in genetics improvement and breeding of Chinese aspen (Populus davidiana Dode) in China. This introduction includes natural distribution and collection, conservation, gene diversity, provenance trial, crossing breeding, vegetative propagation and disease resistant etc. Based on the current situation of forest tree breeding in China, some strategic suggestions concerning the future development of Chinese aspen genetics improvement in China are presented, taking into consideration the existing domestic demands of forestry production and international trends in forest tree breeding.

Progress and Strategies in Cross Breeding of Poplars in China

The advance in intrasection and intersection cross breeding of poplars in China over the past 50 years is reviewed. Great progress has been made in Sections Leuce and Aigeiros, and satisfactory results of intersection hybridization have been achieved in the crossing between Sections Tacamahaca and Aigeiros. The modes of hybridization include single cross, double cross, triple cross, backcross, etc. It is known that using hybrids as parents to cross with other species or hybrids is an effective and easy way to obtain heterosis. Fast growth, cold and drought tolerance, pest and disease resistance, narrow crowns and rootage, etc. are breeding goals. The conventional artificial crossing is still a major breeding method, and a combination of the conventional artificial crossing with physical radiation and chemical induction can create new triploid individuals that possess higher yield potential. The super clones cultivated have already displayed enormous socioeconomic and ecological benefits in practice. Finally, the problems that investigators have to face at present are discussed as well as some strategies in poplar cross breeding in China.

Cross Breeding of Populus and Its Hybrids for Cold Resistance

Populus tomentosa was crossed with P.tremuloidis, P.grandidentata, P.alba×P.grandidentata and P.alba×Ulmuspumila in order to maintain its rapid growth and high wood quality and improve its resistance to cold. Two methods were used to increase the germination rate from 1.5% to 41.1% and the remaining rate from 1.7% to 44.2%. Forty crossing combinations were conducted and 2744 hybrid seedlings were obtained. MX4×P.grandidentata （G-1-58）, MX3×P.tremuloidis （T-44-60）, MX2×P.tremuloidis （1-13-87-37） and MX2×（P.alba×P.grandidentata） were regarded as superior combinations after analysis and selection. Thirty seedlings of these combinations and 11 triploid seedlings identified by counting their chromosomes were selected as super plants.

Breeding of High Yield,High Quality and Disease-Resistant Hybrid Cotton Varieties Hanza 160 and Hanza 1692

The hybrid cotton varieties of Hanza 160 and Hanza 1692 were bred by reciprocal crosses between Han 5158 and Han 333 in Hainan and Hebei for several years. Both varieties had the characters of high yield, high quality and disease resistance. Hanza 160 and Hanza 1692 were approved by Hebei Crop Variety Approval Committee in 2013 and 2016, respectively.

Cross-breeding of Different Geological Populations of Scallop, Chlamys farreri

Wild populations of Chlamys farreri derived from Japan and China are used as parents in a cross breeding experiment conducted in Zhangzi Island of Liaoning Province in the early May of 2000. Four different first filial generations of the crossbreeding, namely JC, CJ, CC and JJ, which represent different cross combinations of the parents, are differentiated from each other in some observed quantitative traits including survival rate, hatchery rate and attachment rate, etc. By ANOVA analysis, significant differences in growth rate are found among the four populations of the offspring after one year of culturing in Shandong Province. JJ population shows the fastest growth rate and has shell height of 46.1±0.71(SD)mm in average, whereas the other three populations, CC, CJ and JC, has the shell height of 43.1±0.42mm, 41.5±0.48mm and 37.2 ±0.31mm, respectively. No heterosis is detected in the experiment.

Current Status of Research on Pollen Vitality and Stigma Receptivity of Wuren Walnut(Juglans sigillata)in Guizhou Province

Wuren walnut is a unique germplasm resource of Juglans sigillata in Guizhou Province,named after its purple and black seed coat.It is a typical medicinal and edible plant with high nutritional value and good taste compared with ordinary walnut.The color of its inner seed coat is different from that of ordinary walnut,which is mostly light yellow.Wuren walnut has great market potential for development.To lay a theoretical foundation for the future breeding of improved varieties,pollination tree configuration,and hybrid breeding of Wuren walnut in Guizhou Province,this paper summarized the current research status of pollen vitality and stigma acceptability of Guizhou Wuren walnut in recent years,and looked forward to future research and application.

Analysis of Protein Expression Patterns of Silkworm Jinqiu and Its Cross Parents

The differences of protein expression between the improved cross breeding race Jinqiu and its parents were analyzed to discuss the gene construction, and to form a base for illuminating the molecular mechanisms of successful cross breeding in silkworm. Protein samples from silk gland, hemolymph, and midgut were separated by 2-dimensional gel electrophoresis （2-DE）. In the three tissues the matched protein spots between Jinqiu and its cross parents were approximately 70% with approximately 30% specific protein spots. In the matched protein spots, 9-24% was differentially expressed representing up- and down-regulated expression. These specific protein spots might be either the newly appeared, which were produced from the genic interaction of cross parents＇ genes in cross breeding, or posttranscriptionally modified, which were produced from the different modifications on the same original proteins. These results indicate that it is important for a new successful breed, by cross breeding, relying on the actions of some newly produced functional proteins from genic interaction, in addition to marshaling excellent genes of cross parents.

Stability of PAC(Psy-2A-Crt I) gene and agronomic traits in the F_(2:3) of IR36/PAC transgenic plants

This study was carried out to evaluate the expression of yellow endosperm color in the progeny of Psy-2A-Crt I（β-carotene gene, PAC） transgenic plants crossed with IR36. The selected 25 lines of F2 progeny exhibited stable expression of yellow endosperm color and high agronomic characteristics. IR36 and PAC transgenic plant of the grain length, width and ratio showed the same tendency of normal distribution. For progeny selection, colorimeters were as employed to distinguish differing visible colors. Correlation analysis was conducted to investigate the relationship between b＊ value and the expression of specific genes. b＊ value was associated with the relative expression of the PAC-Rev and MAR product by real-time PCR and t-test revealed significant difference. Based on this study, correlation between colorimetric values and real-time PCR was effective to detect gene expression. Yellow endosperm progenies represent a new genetic pool that might be useful to increase the genetic diversity of yellow endosperm rice.