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Extraction and Antioxidant Activity Analysis of Crude Polysaccharides from Wild Lactarius volemus Fr. in Yunnan Province 被引量:1
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作者 Meihua XIE Zhongze LUO +2 位作者 Yongtao HU Xiufeng LI Haiyan YANG 《Agricultural Biotechnology》 CAS 2015年第6期53-56,共4页
[ Objective ] This study aimed to investigate the optimal extraction process of crude polysaccharides from wild Lactarius volemus Fr. in Yunnan Province and preliminarily analyzed its antioxidant activity in vitro. [ ... [ Objective ] This study aimed to investigate the optimal extraction process of crude polysaccharides from wild Lactarius volemus Fr. in Yunnan Province and preliminarily analyzed its antioxidant activity in vitro. [ Method ] With water extraction and alcohol precipitation method, the optimal conditions for extracting crude polysaccharides from wild L. volemus Ft. were screened by single-factor and orthogonal experiments. The antioxidant activity of the extracted crude polysac- charides was determined with DPPH assay. [ Result ] The optimal conditions for pigment removal with activated carbon were: activated carbon amount of 20 g/L, water bath time of 40 min, water bath temperature of40 ℃ ; the optimal conditions for extracting crude polysaccharides from wild L. vo/emus Fr. with hot water ex- traction method were: hot water extraction time of 3 h, solid-liquid ratio of 1: 45, extraction frequency of twice. Under the optimized extraction conditions, the yield of crude polysaccharides was 21.33 mg/g. In addition, the antioxidant activity of 0. 665 mg/ml crude polysaccharides was 52.46% ; the amount of crude polysac- eharides was proportional to the antioxidant activity. [ Conclusion] Hot water extraction method can be used as a high-efficiency extraction technology of crude pol- ysaecharides from wild L. volemus Fr. with simple operation and low costs. Crude polysaccharides extracted from L. volemus Ft. exhibited certain antioxidant activi- ty in vitro. 展开更多
关键词 Lactarius volemus Fr. crude polysaccharide Antioxidant activity Extraction method
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Effects of White-Rot Fungi Composting on Wood Powder-Chicken Manure Mixture
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作者 HU Chang-qing HE Xiao-yi SHAO Wei SHAO Sai-nan ZHANG Li-lin 《Animal Husbandry and Feed Science》 CAS 2009年第6期42-46,共5页
[ Objective] To explore the possibility of treating livestock manure with white-rot fungi. [Method] The wood powder-chicken manure mixture was respectively composted by three kinds of white-rot fungi, the weight of li... [ Objective] To explore the possibility of treating livestock manure with white-rot fungi. [Method] The wood powder-chicken manure mixture was respectively composted by three kinds of white-rot fungi, the weight of lignin, cellulose, protein, fat, ash and crude polysaccharide as well as the mycelial growth was determined during this process. [ Result] The mixture was better composted with white-rot fungi than the control. The best effect was achieved in P. ostreatus composting group. In this group, the rate of weight reduction, lignin degradation and cellulose degradation were respectively 15.68% (6.79 times as great as that of the control group), 39.92% (6.54 times as great as that of the control group) and 32.26% (2.77 times as great as that of the control group). The weight of protein and fat were increased by 31.68% and 146.58%, respectively. The content of crude polysaccharide was 2.43%. No crude polysaccharide was detected, and the weight of protein and fat decreased by 21.96% and 70.99%, respectively. [ Conclusion] It is feasible to compost livestock and poultry manure with white-rot fungi. 展开更多
关键词 White-rot fungi Wood powder-chicken manure mixture LIGNIN CELLULOSE crude polysaccharide
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