Establishment of Fingerprint of Cryptolepis buchananii Roem. et Schult. by HPLC

[Objectives]This study aimed to establish the fingerprint of Cryptolepis buchananii Roem.et Schult.by HPLC.[Methods]Taking the peak of chlorogenic acid as the references,the 10 batches of C.buchananii Roem.et Schult.materials were detected by HPLC under the conditions of column of Thermo ODS-2 Hypersil(5μm,4.6 mm×250 mm),detection wavelength of 323 nm,column temperature of 25℃,mobile phase of methanol-0.1%phosphoric acid solution,gradient elution and injection volume of 20μL.The common peak identification and similarity evaluation were carried out according to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Traditional Medicine(Version 2004 A).[Results]Among the HPLC fingerprints of the 10 batches of C.buchananii Roem.et Schult.materials,there are 13 common peaks,and their similarity is all greater than 0.9,indicating good similarity.[Conclusions]This study can provide a scientific basis for the quality evaluation of C.buchananii Roem.et Schult.

Study on Infrared Fingerprint of Cryptolepis buchananii Roem. et Schult.

[Objectives] This study was conducted to establish the infrared fingerprint of Cryptolepis buchananii Roem. et Schult. [Methods] Ten batches of C. buchananii from different habitats were determined by Fourier transform infrared spectroscopy. The experimental data were clustered by Omic software and Ward method. [Results] The infrared fingerprints of C. buchananii were obtained, and the similarity was all greater than 0.9. [Conclusions] The established method has good stability and controllability and can be used for the identification of C. buchananii.

Protective Effect of the Zhuang Medicine Cryptolepis buchananii Roem. et Schult. on D-GalN-induced Liver Injury in Mice

[Objectives] This study was conducted to study the protective effect of the Zhuang medicine Cryptolepis buchananii Roem. et Schult. on D-galactosamine(D-GalN)-induced liver injury in mice. [Methods] A mouse model of chemical liver injury was established by D-GalN, and the mice with D-GalN-induced liver injury were randomly divided into the blank group, model group, positive control group and drug-administered groups. The levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in the serum of the mice in each group and the levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in the liver tissue of the mice were determined, and the liver pathological changes were observed. [Results] The extracts of the Zhuang medicinal herb C. buchananii could reduce the serum ALT and AST levels(P<0.05), increase the SOD content in the liver tissue of mice(P<0.05), and decrease the MDA level(P<0.05). [Conclusions] The Zhuang medicinal herb C. buchananii has a good protective effect on D-GalN-induced liver injury in mice.

古钩藤水提液对小鼠实验性高血糖影响的实验研究

目的探讨古钩藤水提液对小鼠实验性高血糖的影响。方法建立链脲佐菌素和四氧嘧啶诱导小鼠高血糖模型,按高、中、低剂量的古钩藤水提液灌胃给药,空白对照组和模型对照组给予等体积的生理盐水,阳性对照组给予盐酸二甲双胍、格列本脲,观察给药后小鼠血糖的变化。结果给药前模型对照组、格列本脲组、古钩藤高剂量组、中剂量组、低剂量组5组之间血糖值比较,差异无统计学意义(P>0.05);给药后古钩藤高剂量组、中剂量组、低剂量组血糖值均明显低于链脲佐菌素模型对照组(P<0.05)。给药前模型对照组、二甲双胍组,以及古钩藤高剂量组、中剂量组、低剂量组5组之间血糖值比较,差异无统计学意义(P>0.05);给药后古钩藤高剂量组、中剂量组、低剂量组血糖值均明显低于四氧嘧啶模型对照组(P<0.05)。结论古钩藤水提液对链脲佐菌素致高血糖小鼠和四氧嘧啶致高血糖小鼠都有显著降血糖作用。

古钩藤茎叶的化学成分研究

目的研究民族药用植物古钩藤Cryptolepis buchananii茎叶的化学成分。方法采用硅胶、Sephadex LH-20、ODS柱色谱以及制备HPLC等手段分离并纯化化合物,通过NMR、MS等波谱技术并结合文献数据鉴定化合物结构。结果从古钩藤茎叶甲醇提取物的醋酸乙酯部分共分离得到13个化合物,分别鉴定为异莨菪亭(1)、(+)-3-hydroxy-β-ionone(2)、(3R,6R,7E)-3-hydroxy-4,7-megastigmadien-9-one(3)、ficusic acid(4)、(+)-松脂素(5)、(+)-8-羟基松脂素(6)、(+)-丁香脂素(7)、异橙黄胡椒酰胺乙酸脂(8)、loliolide(9)、(–)-蛇菰宁(10)、金圣草素(11)、9-hydroxy-10E,12Z-octadecadienoic acid methyl ester(12)和ficusesquilignan A(13)。结论所有化合物均为首次从该属植物中分离得到。