To introduce CMV resistance to susceptible variety, interspecific grafting between d45-6 (Capsicum annuum) and LS1205 (C. baccatum) was conducted. The graft-progenies, G1 and G1S1 were obtained. Mechanical inoculation...To introduce CMV resistance to susceptible variety, interspecific grafting between d45-6 (Capsicum annuum) and LS1205 (C. baccatum) was conducted. The graft-progenies, G1 and G1S1 were obtained. Mechanical inoculation with CMV showed that disease index of G1 and G1S1 were significantly decreased, similar to that of the resistant stock. ELISA serological test indicated that resistance of LS1205 and the graft-progenies to CMV was caused by inhibition of virul replication. Further, peroxidase isozymatic analysis revealed that zymotypes of G1 were a summation of those found in the donor plants. This result perfectly fit the theory of gene transformation and reconfirmed the conclusion of mechanism of graft-induced variants.展开更多
Cucumber mosaic virus （CMV）, a positive-sense single-stranded RNA virus, has a very wide host range and a worldwide distribution, and is considered as one of the most virulent plant viruses in the world. The CMV gen...Cucumber mosaic virus （CMV）, a positive-sense single-stranded RNA virus, has a very wide host range and a worldwide distribution, and is considered as one of the most virulent plant viruses in the world. The CMV gene sequences, gene products, and their interaction with hosts have been extensively reported since it was discovered in 1916. With the development of high-throughput sequencing and proteomics, great progress has been made in the molecular mechanism of CMV pathogenesis in recent years. In this review, we introduce CMV-encoded proteins, CMV-related satellite RNAs and the roles of host factors in the pathogenesis of CMV, to provide a theoretical basis for future study of CMV.展开更多
Cucumber mosaic virus（CMV） can infect a wide range of host species. For the lacking of CMV resistant varieties of tomato, RNA interference（RNAi） can be used as a fast and effective method for the generation of tra...Cucumber mosaic virus（CMV） can infect a wide range of host species. For the lacking of CMV resistant varieties of tomato, RNA interference（RNAi） can be used as a fast and effective method for the generation of transgenic resistant varieties. In this current study, five intron-spliced hairpin RNA（ihp RNA） plant expression vectors aimed at five genes of CMV have been constructed. Transgenic tomatoes were obtained by Agrobacterium tumefaciens-mediated transformation with expression vectors. Highly resistant generations of transgenic plants were employed as rootstocks and grafted onto non-transgenic tomatoes that resulted in the successful transfer of resistance to the scions. Using a novel method of plant cuttings for rootstock propagation, we obtained large quantities of disease-resistant material. Further, this method produces scions that can remain undetectable for transgenic resistance marker genes that may provide novel approaches to evade collective concerns about genetically-modified organism（GMO） biosafety.展开更多
文摘To introduce CMV resistance to susceptible variety, interspecific grafting between d45-6 (Capsicum annuum) and LS1205 (C. baccatum) was conducted. The graft-progenies, G1 and G1S1 were obtained. Mechanical inoculation with CMV showed that disease index of G1 and G1S1 were significantly decreased, similar to that of the resistant stock. ELISA serological test indicated that resistance of LS1205 and the graft-progenies to CMV was caused by inhibition of virul replication. Further, peroxidase isozymatic analysis revealed that zymotypes of G1 were a summation of those found in the donor plants. This result perfectly fit the theory of gene transformation and reconfirmed the conclusion of mechanism of graft-induced variants.
基金Supported by the Special Fund for Basic Scientific Research of Chinese Academy of Inspection and Quarantine(2017JK035)
文摘Cucumber mosaic virus （CMV）, a positive-sense single-stranded RNA virus, has a very wide host range and a worldwide distribution, and is considered as one of the most virulent plant viruses in the world. The CMV gene sequences, gene products, and their interaction with hosts have been extensively reported since it was discovered in 1916. With the development of high-throughput sequencing and proteomics, great progress has been made in the molecular mechanism of CMV pathogenesis in recent years. In this review, we introduce CMV-encoded proteins, CMV-related satellite RNAs and the roles of host factors in the pathogenesis of CMV, to provide a theoretical basis for future study of CMV.
基金supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China(20134320120013)the Natural Science Foundation of Hunan Province of China(14JJ3095)
文摘Cucumber mosaic virus（CMV） can infect a wide range of host species. For the lacking of CMV resistant varieties of tomato, RNA interference（RNAi） can be used as a fast and effective method for the generation of transgenic resistant varieties. In this current study, five intron-spliced hairpin RNA（ihp RNA） plant expression vectors aimed at five genes of CMV have been constructed. Transgenic tomatoes were obtained by Agrobacterium tumefaciens-mediated transformation with expression vectors. Highly resistant generations of transgenic plants were employed as rootstocks and grafted onto non-transgenic tomatoes that resulted in the successful transfer of resistance to the scions. Using a novel method of plant cuttings for rootstock propagation, we obtained large quantities of disease-resistant material. Further, this method produces scions that can remain undetectable for transgenic resistance marker genes that may provide novel approaches to evade collective concerns about genetically-modified organism（GMO） biosafety.
