Molecular marker for screening rice cultivars with intermediate amylose content in indica

The waxy(Wx)gene of rice encodes the granule-bound starch synthase(GBSS),which was required in the synthesis of amylose in endosperm.Theamylose content(AC)of rice endosperm played an important role in grain yield,palatability,and processing quality.Our previous researehes showed that en-dosperm AC and GBSS contents were correlated with the ability of excising intron1 from the leader sequence of the Wx transcript.Cultivars with high endosperm

Genotype Screening of Recipient Resources with High Regeneration and Transformation Efficiency in Chrysanthemum

Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum,which relies on an optimal regeneration and transformation system.However,the regeneration system of different chrysanthemum cultivars varies,and the regeneration time of most cultivars is long.To screen cultivars with highly efficient regeneration,leaves and shoot tip thin cell layers(tTCL)from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media(MS)supplemented with 1.0-5.0 mg L^(−1)6-benzylaminopurine(6-BA)and 0.1-1.0 mg L^(−1)α-naphthaleneacetic(NAA).The results showed that the most efficient regeneration media were MS+6-BA 1.0 mg L^(−1)+NAA 0.5 mg L^(−1)for leaf explants and MS+6-BA 5.0 mg L^(−1)+NAA 0.1 mg L^(−1)for tTCL explants.Subsequently,another 13 chrysanthemum cultivars were screened by using the media,and finally,three cultivars with high regeneration efficiency were obtained from 21 cultivars.Among these,C1 had the highest regeneration efficiency:the regeneration rate of leaf explants reached 80.0%after 42 days of culture,and the regeneration rate of tTCL explants reached 100%after 31 days of culture.Furthermore,we also established the transformation system for C1 as follows:preculturing for one day,infecting with Agrobacterium suspension(OD_(600)=0.6)for 10 min,and cultivating in the regeneration medium with 350 mg L^(−1)carbenicillin and 10 mg L^(−1)kanamycin,thus ultimately achieving a transformation rate of 4.0%.In this study,a new chrysanthemum cultivar with an efficient regeneration and transformation system was screened,which is beneficial to enrich the flower color of chrysanthemum transgenic plant recipients and to the functional research of flower color or type-related genes.

Observation and Evaluation of New Lily Cutflower Varieties Introduced in Tropical Area

[Objective] New lily varieties were introduced and selected to provide ref- erences for choosing lily varieties with the best comprehensive performance and most appropriate for tropical area. [Method] In winter, six lily cultivars including White Express, Concad＇or, Aktiva, Donato, Star Fighter and Serano were planted outdoors in Hainan. Phenophase, morphological traits and cut flower quality of the tested cultivars were observed and compared in order to choose varieties suitable for Hainan. [Result] Their growth period ranged from 96 to 113 d with the mean of 105.3 d. Six cultivars had obvious differences in biological characteristics and cut- flower quality. Compared with the other 4 cultivars, Aktiva and Serano were superior for their bright color and beautiful shape, height uniformity, long florescence and strong growing. According to the comprehensive evaluation system of lily variety, the sequence was Serano 〉 Aktiva 〉 Concad＇or 〉 Star Fighter 〉 Donato 〉 White Ex- press. [Conclusion] The two cultivars of Aktiva and Serano with good characters are selected for further research and extension in Sanya of Hainan.