[Objective] The research aimed to enhance culture efficiencies of oocyte and embryo of goat in vitro and to explore serum-free culture system in vitro.[Method] At present,the conventional solutions of oocyte maturatio...[Objective] The research aimed to enhance culture efficiencies of oocyte and embryo of goat in vitro and to explore serum-free culture system in vitro.[Method] At present,the conventional solutions of oocyte maturation and embryo development in vitro were always added into 1% ITS(Insulin-transferrin-selenium) or using 1% ITS to replace FBS in 2 kinds culture solutions for conducting in vitro cultures of goat oocyte and parthenogenetic embryo.The influences of ITS on their developments were detected.[Result] ITS in maturation liquid of oocytes could not increase oocytes maturation rate but significantly increased blastocyst rate (58.06% vs. 48.19%)of parthenogenetic embryo.If FBS in maturation liquid of oocytes was replaced by ITS, the maturation rate, cleavage rate and blastocyst rate were basically unchanged.Adding ITS into embryo medium could increase blastocyst rate (68.30% vs. 56.82%)of parthenogenetic embryo of goat.If FBS in embryo medium was replaced by ITS,the cleavage rate didn’t change basically,while the blastocyst rate in ITS was obviously lower than that in FBS group(42.33% vs.56.82%).[Conclusion] ITS could promote maturation of oocyte in vitro and early embryonic development, in addition,ITS could replace serum in maturation medium of oocyte as serum-free culture system for conducting relevant researches.展开更多
目的：探索合适的小凹制作方法以培养小鼠无透明带胚胎，完善WOW（the well of the well）系统，为手工克隆技术提供支持，提高核移植生产效率．方法：对WOW系统进行改进，用金属针、玻璃针及胚胎聚集针分别做小凹，比较这三种方法制作...目的：探索合适的小凹制作方法以培养小鼠无透明带胚胎，完善WOW（the well of the well）系统，为手工克隆技术提供支持，提高核移植生产效率．方法：对WOW系统进行改进，用金属针、玻璃针及胚胎聚集针分别做小凹，比较这三种方法制作的小凹对小鼠无透明带受精胚胎的培养效果．结果：胚胎聚集针所做小凹的囊胚发育率显著高于金属针及玻璃针所做小凹（55．61％vs44．38％，43．16％）．结论：胚胎聚集针制作的小凹培养小鼠无透明带受精胚胎，囊胚发育率最高．展开更多
文摘[Objective] The research aimed to enhance culture efficiencies of oocyte and embryo of goat in vitro and to explore serum-free culture system in vitro.[Method] At present,the conventional solutions of oocyte maturation and embryo development in vitro were always added into 1% ITS(Insulin-transferrin-selenium) or using 1% ITS to replace FBS in 2 kinds culture solutions for conducting in vitro cultures of goat oocyte and parthenogenetic embryo.The influences of ITS on their developments were detected.[Result] ITS in maturation liquid of oocytes could not increase oocytes maturation rate but significantly increased blastocyst rate (58.06% vs. 48.19%)of parthenogenetic embryo.If FBS in maturation liquid of oocytes was replaced by ITS, the maturation rate, cleavage rate and blastocyst rate were basically unchanged.Adding ITS into embryo medium could increase blastocyst rate (68.30% vs. 56.82%)of parthenogenetic embryo of goat.If FBS in embryo medium was replaced by ITS,the cleavage rate didn’t change basically,while the blastocyst rate in ITS was obviously lower than that in FBS group(42.33% vs.56.82%).[Conclusion] ITS could promote maturation of oocyte in vitro and early embryonic development, in addition,ITS could replace serum in maturation medium of oocyte as serum-free culture system for conducting relevant researches.
文摘目的：探索合适的小凹制作方法以培养小鼠无透明带胚胎，完善WOW（the well of the well）系统，为手工克隆技术提供支持，提高核移植生产效率．方法：对WOW系统进行改进，用金属针、玻璃针及胚胎聚集针分别做小凹，比较这三种方法制作的小凹对小鼠无透明带受精胚胎的培养效果．结果：胚胎聚集针所做小凹的囊胚发育率显著高于金属针及玻璃针所做小凹（55．61％vs44．38％，43．16％）．结论：胚胎聚集针制作的小凹培养小鼠无透明带受精胚胎，囊胚发育率最高．
