Propagation of Abies beshanzuensis by Water Cultured Medium

[Objective] The experiment aimed to explore the influences of phytohormones (ABT and IAA) and nutrient solution on rooting of Abies beshanzuensis M.H.Wu by water cultured medium. [Method] The Abies beshanzuensis M.H.Wu were treated by water (CK), 10 mg/L ABT+ water, 10 mg/L IAA+ water, 10 mg/L ABT+ hoagland solution, 10 mg/L IAA+ hoagland solution, then the rooting process was observed and the formation rate of callus, rooting rate, number of rooting, and root length were investigated and analyzed. [Result] ABT and IAA had obvious influences on callus induction, rooting rate and the number of root of Abies beshanzuensis M.H.Wu by water culture, so they were suitable to be used in water propagation of Abies beshanzuensis M.H.Wu. The treatments of phytohormones had no regular influences on the longest root length and average root length. The nutrient solutions would not generate obvious influence on propagation of Abies beshanzuensis M.H.Wu at firstly stage, but they generated influence on root growth after rooting. [Conclusion] The research provided new ideas for propagation of Abies beshanzuensis M.H.Wu, which could make it out of endangerment situation quickly.

Tissue Culture Rapid Propagation and Transplantation Techniques of Anoectochilus roxburghii (Wall) Lind.

[Objectives]To screen the tissue culture rapid propagation formula suitable for each tissue culture stage of Anoectochilus roxburghii( Wall) Lind. [Methods] The stem segments of Fujian A. roxburghii were used as explant to study the tissue culture rapid propagation and transplantation techniques. The comparative experiment was carried out to study the effects of different hormone concentrations on the induction of stem segments,proliferation of cluster buds,rooting and seedling hardening of A. roxburghii,and study the effects of transplantation matrix on the transplantation of A. roxburghii. [Results]MS + 0. 5 mg/L NAA + 2 mg/L BA + 20 g/L sucrose + 6 g/L agar was suitable for induction of stem segments of A. roxburghii; MS + 0. 5 mg/L NAA + 2 mg/L BA + 1 mg/L KT + 25 g/L sucrose + 6 g/L agar was most suitable for proliferation of cluster buds of A. roxburghii; MS + 1. 0 mg/L IBA + 1. 0 mg/L NAA + 1 g/L activated carbon + 50 g/L mashed banana + 25 g/L sucrose + 6 g/L agar was most suitable for rooting and seedling hardening of A. roxburghii; using peat soil: fine sand( 3∶ 1) as transplantation matrix,the survival rate was the highest. [Conclusions] The experiment results are expected to provide references for factory production of A. roxburghii.

The Enlightenment of the Concept of Cultural Confidence on International Chinese Education and Cultural Propagation

Cultural self-confidence is the full affirmation and active practice of a people,a country and a political party of its own cultural value,and the firm confidence in its cultural vitality.As an important hub and window for cultural exchanges between China and foreign countries,international Chinese education shoulders the important task of language and cultural propagation.In the mutual learning and exchanges between Chinese and foreign cultures,international Chinese education must insist on cultural self-confidence in teachers,teaching,teaching materials,etc.,"tell Chinese stories and spread Chinese voice",and show the world a rich,stereo,and vivid image of China.

Rapid Progation Technique of New Variety of Asparagus--Linlu No.1

This study explored the medium for new variety of Asparagus——Linlu No.1 to induce germination, bud proliferation,and induce rootings and their formula. The result revealed that MS+6-BA 1.0 mg/L+NAA 0.05 mg/L is the suitable medium for germination; MS+6-BA 0.5 mg/L+NAA 0.5 mg/L is the medium fit for bud proliferation and 1/2MS+IBA 0.5 mg/L+KT 0.05 mg/L+sucrose 30 g/L is the perfect medium for rootings.