The genes coding for the α-and β-subunit of allophycocyanin (apcA and apcB) fromthe cyanophyte Spirulina maxima were cloned and sequenced. The results revealed 44.4% of nucleotidesequence similarity and 30.4% of sim...The genes coding for the α-and β-subunit of allophycocyanin (apcA and apcB) fromthe cyanophyte Spirulina maxima were cloned and sequenced. The results revealed 44.4% of nucleotidesequence similarity and 30.4% of similarity of deduced amino acid sequence between them. The aminoacid sequence identities between S. maxima and S. platensis are 99 .4% for α subunit and 100% for βsubunit.展开更多
Liver glutathione S-transferase(GST) plays a major role in the detoxification of microcystins(MCs) via conjugation to glutathione(GSH).We evaluated the relationship between seasonal variation in fish gut contents and ...Liver glutathione S-transferase(GST) plays a major role in the detoxification of microcystins(MCs) via conjugation to glutathione(GSH).We evaluated the relationship between seasonal variation in fish gut contents and the expression of GST isoforms in mud carp(Cirrhina molitorella) and Nile tilapia(Oreochromis niloticus).We quantified the abundance and diversity of plankton in the water column and foregut of mud carp and Nile tilapia in the tropical Xiangang Reservoir between October 2007 and July 2008.The mRNA expression of 7 liver GST isoforms was determined by real-time RT-PCR.The gut contents of both species were dependent on the amount and type of phytoplankton and zooplankton in the water.The expression of liver GST genes in Nile tilapia and mud carp was positively and negatively correlated,respectively,with the abundance of toxic cyanobacteria in the fore-gut.The expression of liver GST mRNA was correlated to the abundance of toxic cyanobacteria in the gut contents of both species,suggesting that mRNA expression of GST isoforms could be used as a biomarker in Nile tilapia and mud carp to monitor cyanobacteria blooms in reservoirs.展开更多
文摘The genes coding for the α-and β-subunit of allophycocyanin (apcA and apcB) fromthe cyanophyte Spirulina maxima were cloned and sequenced. The results revealed 44.4% of nucleotidesequence similarity and 30.4% of similarity of deduced amino acid sequence between them. The aminoacid sequence identities between S. maxima and S. platensis are 99 .4% for α subunit and 100% for βsubunit.
基金supported by the National High Technology Research and Development Program of China (2007AA09Z437)the Fundamental Research Funds for the Central Universities (2010PY010,2011PY030)
文摘Liver glutathione S-transferase(GST) plays a major role in the detoxification of microcystins(MCs) via conjugation to glutathione(GSH).We evaluated the relationship between seasonal variation in fish gut contents and the expression of GST isoforms in mud carp(Cirrhina molitorella) and Nile tilapia(Oreochromis niloticus).We quantified the abundance and diversity of plankton in the water column and foregut of mud carp and Nile tilapia in the tropical Xiangang Reservoir between October 2007 and July 2008.The mRNA expression of 7 liver GST isoforms was determined by real-time RT-PCR.The gut contents of both species were dependent on the amount and type of phytoplankton and zooplankton in the water.The expression of liver GST genes in Nile tilapia and mud carp was positively and negatively correlated,respectively,with the abundance of toxic cyanobacteria in the fore-gut.The expression of liver GST mRNA was correlated to the abundance of toxic cyanobacteria in the gut contents of both species,suggesting that mRNA expression of GST isoforms could be used as a biomarker in Nile tilapia and mud carp to monitor cyanobacteria blooms in reservoirs.
