The codling moth, Cydia pomonella L. （Lepidoptera： Tortricidae）, is a destructive insect pest in the fruit production. The emergences of this notorious pest frequently cause the huge economic loss of apple orchards...The codling moth, Cydia pomonella L. （Lepidoptera： Tortricidae）, is a destructive insect pest in the fruit production. The emergences of this notorious pest frequently cause the huge economic loss of apple orchards. It can also attacks pears, walnuts and other tree fruits. The codling moth is an important invasive species in China, which first appeared in Kuerle, Xijiang in 1953 and rapidly expanded in the whole area, in 1987 the codling moth spread to Dunhuang, Gansu. Recently, researchers from the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Nanjing Agricultural University and so on initiated a genome-sequencing project of the codling moth. The genome size of the codling moth is 650 Mb, as estimated by flow cytometry and survey sequencing. Since the heterozygousity is a potential obstacle in sequencing the insect genome, the research consortium carefully measured the heterozygousity of the codling moth by SNP analysis and 17-mer estimation, showing that the heterozygousity of the codling moth is around 0.3-0.6%. The whole genome shotgun strategy is adopted and a sequencing plan has been made and officially started. The availability of the codling moth genome should be of great value to uncover the molecular invasion mechanism, high adaptive ability to the stress and to develop efficient control strategies of this pest worldwide and in China.展开更多
Juvenile hormone esterase(JHE) is a key enzyme for insects,playing an important role in the regulation of insect growth,development,diapause and reproduction.We identified a complete putative JHE of Cydia pomonella(Cp...Juvenile hormone esterase(JHE) is a key enzyme for insects,playing an important role in the regulation of insect growth,development,diapause and reproduction.We identified a complete putative JHE of Cydia pomonella(CpJHE-like) which is comprised of a 1 761 bp coding sequence(CDS) encoding 587 amino acid residues from the transcriptome data.The deduced protein sequence of CpJHE-like showed the highest identity of 60.44% with the Adoxophyes honmai JHE(AhJHE) and the minimal identity of 25.81% with Aedes aegypti JHE(AaJHE).CpJHE-like exhibited all the seven typical motifs of the functional JHEs and had the highly consistent tertiary structure with Manduca sexta JHE(MsJHE).Phylogenetic analysis showed that the CpJHE-like was close to two JHEs from the family Tortricidae.The CpJHE-like transcript level take a leap in the 3-day-old fifth instar larva,increased about 300-fold compared to the basal level.Tissue-specific expression profile showed that the CpJHE-like transcript was expressed mainly in the fat body.This study indicates that the CpJHE-like is the functional JHE,which may play vital roles in the development and reproduction of C.pomonella.展开更多
Seasonal changes in the fatty acid composition of the total lipid extracted from the whole body of Cydia pomonella L. larvae were determined by gas chromatography. The six most abundant fatty acids in both non-diapaus...Seasonal changes in the fatty acid composition of the total lipid extracted from the whole body of Cydia pomonella L. larvae were determined by gas chromatography. The six most abundant fatty acids in both non-diapause and diapause larvae of codling moth were oleic （35%-39%）, palmitic （23%-33%）, linoleic （16%-30%）, palmitoleic （5%-10%）, stearic （1.5%-3.0%） and linolenic acids （1.0%-2.5%）. This represents a typical complement of Lepidopteran fatty acids. The fatty acid composition of total lipid of C. pomonella larvae was related to diapause. In similarity to most other reports, the proportion of unsaturated fatty acids increased in diapause initiation state. The total lipid of diapause larvae contained more linoleic acid （25.8% vs. 16.1%） and less palmitic acid （24.7% vs. 33.4%）, than that of non-diapause larvae. The weight percentage of linoleic acid （C 18：2） increased from 16% to 26% from early-August through early-September during transition to diapause, while palmitic acid （C16：0） decreased from 33% to 25% at the same time. These changes resulted in an increase in the ratio of unsaturated to saturated fatty acids （UFA/SFA） from 1.72 in non-diapause larvae to 2.63 in diapause larvae.展开更多
文摘The codling moth, Cydia pomonella L. （Lepidoptera： Tortricidae）, is a destructive insect pest in the fruit production. The emergences of this notorious pest frequently cause the huge economic loss of apple orchards. It can also attacks pears, walnuts and other tree fruits. The codling moth is an important invasive species in China, which first appeared in Kuerle, Xijiang in 1953 and rapidly expanded in the whole area, in 1987 the codling moth spread to Dunhuang, Gansu. Recently, researchers from the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Nanjing Agricultural University and so on initiated a genome-sequencing project of the codling moth. The genome size of the codling moth is 650 Mb, as estimated by flow cytometry and survey sequencing. Since the heterozygousity is a potential obstacle in sequencing the insect genome, the research consortium carefully measured the heterozygousity of the codling moth by SNP analysis and 17-mer estimation, showing that the heterozygousity of the codling moth is around 0.3-0.6%. The whole genome shotgun strategy is adopted and a sequencing plan has been made and officially started. The availability of the codling moth genome should be of great value to uncover the molecular invasion mechanism, high adaptive ability to the stress and to develop efficient control strategies of this pest worldwide and in China.
基金supported by the National Key Research and Development Program of China(2016YFC1201200,2017YFC1200600 and 2016YFC1200602)the Basic Research on Science and Technology Project of Shenzhen,China(JCYJ20160530191934833)the Dapeng New District Industrial Development Special Fund of Shenzhen,China(KY20180215)
文摘Juvenile hormone esterase(JHE) is a key enzyme for insects,playing an important role in the regulation of insect growth,development,diapause and reproduction.We identified a complete putative JHE of Cydia pomonella(CpJHE-like) which is comprised of a 1 761 bp coding sequence(CDS) encoding 587 amino acid residues from the transcriptome data.The deduced protein sequence of CpJHE-like showed the highest identity of 60.44% with the Adoxophyes honmai JHE(AhJHE) and the minimal identity of 25.81% with Aedes aegypti JHE(AaJHE).CpJHE-like exhibited all the seven typical motifs of the functional JHEs and had the highly consistent tertiary structure with Manduca sexta JHE(MsJHE).Phylogenetic analysis showed that the CpJHE-like was close to two JHEs from the family Tortricidae.The CpJHE-like transcript level take a leap in the 3-day-old fifth instar larva,increased about 300-fold compared to the basal level.Tissue-specific expression profile showed that the CpJHE-like transcript was expressed mainly in the fat body.This study indicates that the CpJHE-like is the functional JHE,which may play vital roles in the development and reproduction of C.pomonella.
文摘Seasonal changes in the fatty acid composition of the total lipid extracted from the whole body of Cydia pomonella L. larvae were determined by gas chromatography. The six most abundant fatty acids in both non-diapause and diapause larvae of codling moth were oleic （35%-39%）, palmitic （23%-33%）, linoleic （16%-30%）, palmitoleic （5%-10%）, stearic （1.5%-3.0%） and linolenic acids （1.0%-2.5%）. This represents a typical complement of Lepidopteran fatty acids. The fatty acid composition of total lipid of C. pomonella larvae was related to diapause. In similarity to most other reports, the proportion of unsaturated fatty acids increased in diapause initiation state. The total lipid of diapause larvae contained more linoleic acid （25.8% vs. 16.1%） and less palmitic acid （24.7% vs. 33.4%）, than that of non-diapause larvae. The weight percentage of linoleic acid （C 18：2） increased from 16% to 26% from early-August through early-September during transition to diapause, while palmitic acid （C16：0） decreased from 33% to 25% at the same time. These changes resulted in an increase in the ratio of unsaturated to saturated fatty acids （UFA/SFA） from 1.72 in non-diapause larvae to 2.63 in diapause larvae.
