[Objectives]To explore the effects of homoharringtonine(HHT)on the proliferation and apoptosis of melanoma cells.[Methods]Mouse melanoma cell line B16F10 was divided into 5 dfferent dose groups,among which Oμg/mL HHT...[Objectives]To explore the effects of homoharringtonine(HHT)on the proliferation and apoptosis of melanoma cells.[Methods]Mouse melanoma cell line B16F10 was divided into 5 dfferent dose groups,among which Oμg/mL HHT was as the negative blank group,and the remaining four groups were added with different concentrations of HHT(20,40,80 and i60μg/mL).After B16F10 cells were treated with HHT for 48 h,the growth inhibition rate of B16F10 cells was observed,and the cell cycle was detected.Cyclin D1,MMP-9 and VEGF mRNA and protein levels were detected by qRT-PCR and Western-blot.[Results]Compared with Oμg/mL group,after different concentrations of HHT treated B16F10 cells,the inhibition rate and apoptosis rate of B16F10 cells increased,and Cyclin D1,MMP-9 and VECF mRNA and protein levels in all groups decreased significantly,with statistical significance(P<0.05).[Conclusions]HHT may be involved in the apoptosis of melanoma cells by regulating Cyclin DI level,affecting cell cycle,and inhibiting VEGF and MMP-9 gene protein levels.展开更多
目的:探讨凋亡抑制蛋白surv iv in、抑癌基因PTEN、细胞周期蛋白Cyclin D 1在子宫颈癌中的表达及其临床意义。方法:应用免疫组化SP法,检测surv iv in、PTEN及Cyclin D 1在52例子宫颈癌(鳞癌46例、腺癌6例)及10例正常子宫颈组织中的表达...目的:探讨凋亡抑制蛋白surv iv in、抑癌基因PTEN、细胞周期蛋白Cyclin D 1在子宫颈癌中的表达及其临床意义。方法:应用免疫组化SP法,检测surv iv in、PTEN及Cyclin D 1在52例子宫颈癌(鳞癌46例、腺癌6例)及10例正常子宫颈组织中的表达。结果:与正常子宫颈组织比较,surv iv in与Cy linD 1表达率明显增高,而PTEN的表达率明显降低,差异有统计学意义(P<0.05)。surv iv in和Cyclin D 1蛋白的表达与子宫颈癌的病理分级呈正相关(r值分别为0.57和0.67,P<0.05)。而PTEN的表达病理分级呈负相关(r=0.61,P<0.05)。结论:surv iv in,Cyclin D 1对子宫颈癌的发生、发展起着促进作用,而PTEN的表达对子宫颈癌的进展起着阻碍作用。展开更多
目的:分析中药香加皮醇提物杠柳苷(periplocin from cortex periplocae,CPP)对食管癌细胞株TE-13的生长抑制作用,探讨其诱导细胞周期阻滞的机制。方法:应用MTT法检测CPP对TE-13细胞的抑制作用;Gimsa染色法分析TE-13细胞的形态学变化;FC...目的:分析中药香加皮醇提物杠柳苷(periplocin from cortex periplocae,CPP)对食管癌细胞株TE-13的生长抑制作用,探讨其诱导细胞周期阻滞的机制。方法:应用MTT法检测CPP对TE-13细胞的抑制作用;Gimsa染色法分析TE-13细胞的形态学变化;FCM法检测细胞周期分布和凋亡率;Western印迹法检测TE-13细胞经药物处理前后细胞周期蛋白依赖性激酶CDK4、CDK2蛋白表达的变化。结果:CPP对TE-13细胞增殖具有明显的抑制作用(P<0.01),并呈时间和浓度依赖性,药物浓度越大,作用时间越长,抑制效应越强,CPP作用48h时,对TE-13细胞的半数抑制浓度(IC50)为0.61μg/mL。经2μg/mL的CPP作用48h后,TE-13细胞发生明显的凋亡形态学变化;G0/G1期细胞明显增多(P<0.01),S期细胞明显减少(P<0.01),G2/M期细胞没有明显变化(P>0.05)。不同浓度CPP作用48h后能降低TE-13细胞中CDK4蛋白的表达(P<0.01),对CDK2蛋白的表达则没有明显影响(P>0.05)。结论:CPP能显著抑制TE-13细胞的增殖,其作用机制可能与CPP诱导细胞周期阻滞和细胞凋亡有关。展开更多
基金Supported by the Science and Technology Planning Project of Health Commission of Jiangxi Province(SKJP220229261).
文摘[Objectives]To explore the effects of homoharringtonine(HHT)on the proliferation and apoptosis of melanoma cells.[Methods]Mouse melanoma cell line B16F10 was divided into 5 dfferent dose groups,among which Oμg/mL HHT was as the negative blank group,and the remaining four groups were added with different concentrations of HHT(20,40,80 and i60μg/mL).After B16F10 cells were treated with HHT for 48 h,the growth inhibition rate of B16F10 cells was observed,and the cell cycle was detected.Cyclin D1,MMP-9 and VEGF mRNA and protein levels were detected by qRT-PCR and Western-blot.[Results]Compared with Oμg/mL group,after different concentrations of HHT treated B16F10 cells,the inhibition rate and apoptosis rate of B16F10 cells increased,and Cyclin D1,MMP-9 and VECF mRNA and protein levels in all groups decreased significantly,with statistical significance(P<0.05).[Conclusions]HHT may be involved in the apoptosis of melanoma cells by regulating Cyclin DI level,affecting cell cycle,and inhibiting VEGF and MMP-9 gene protein levels.
文摘目的:探讨凋亡抑制蛋白surv iv in、抑癌基因PTEN、细胞周期蛋白Cyclin D 1在子宫颈癌中的表达及其临床意义。方法:应用免疫组化SP法,检测surv iv in、PTEN及Cyclin D 1在52例子宫颈癌(鳞癌46例、腺癌6例)及10例正常子宫颈组织中的表达。结果:与正常子宫颈组织比较,surv iv in与Cy linD 1表达率明显增高,而PTEN的表达率明显降低,差异有统计学意义(P<0.05)。surv iv in和Cyclin D 1蛋白的表达与子宫颈癌的病理分级呈正相关(r值分别为0.57和0.67,P<0.05)。而PTEN的表达病理分级呈负相关(r=0.61,P<0.05)。结论:surv iv in,Cyclin D 1对子宫颈癌的发生、发展起着促进作用,而PTEN的表达对子宫颈癌的进展起着阻碍作用。
文摘目的:分析中药香加皮醇提物杠柳苷(periplocin from cortex periplocae,CPP)对食管癌细胞株TE-13的生长抑制作用,探讨其诱导细胞周期阻滞的机制。方法:应用MTT法检测CPP对TE-13细胞的抑制作用;Gimsa染色法分析TE-13细胞的形态学变化;FCM法检测细胞周期分布和凋亡率;Western印迹法检测TE-13细胞经药物处理前后细胞周期蛋白依赖性激酶CDK4、CDK2蛋白表达的变化。结果:CPP对TE-13细胞增殖具有明显的抑制作用(P<0.01),并呈时间和浓度依赖性,药物浓度越大,作用时间越长,抑制效应越强,CPP作用48h时,对TE-13细胞的半数抑制浓度(IC50)为0.61μg/mL。经2μg/mL的CPP作用48h后,TE-13细胞发生明显的凋亡形态学变化;G0/G1期细胞明显增多(P<0.01),S期细胞明显减少(P<0.01),G2/M期细胞没有明显变化(P>0.05)。不同浓度CPP作用48h后能降低TE-13细胞中CDK4蛋白的表达(P<0.01),对CDK2蛋白的表达则没有明显影响(P>0.05)。结论:CPP能显著抑制TE-13细胞的增殖,其作用机制可能与CPP诱导细胞周期阻滞和细胞凋亡有关。
