Cymbidium(Orchidaceae:Epidendroideae),with around 60 species,is widely-distributed across Southeast Asia,providing a nice system for studying the processes that underlie patterns of biodiversity in the region.However,...Cymbidium(Orchidaceae:Epidendroideae),with around 60 species,is widely-distributed across Southeast Asia,providing a nice system for studying the processes that underlie patterns of biodiversity in the region.However,phylogenetic relationships of Cymbidium have not been well resolved,hampering investigations of species diversification and the biogeographical history of this genus.In this study,we construct a plastome phylogeny of 56 Cymbidium species,with four well-resolved major clades,which provides a framework for biogeographical and diversification rate analyses.Molecular dating and biogeographical analyses show that Cymbidium likely originated in the region spanning northern IndoBurma to the eastern Himalayas during the early Miocene(~21.10 Ma).It then rapidly diversified into four major clades in East Asia within approximately a million years during the middle Miocene.Cymbidium spp.migration to the adjacent regions(Borneo,Philippines,and Sulawesi)primarily occurred during the Pliocene-Pleistocene period.Our analyses indicate that the net diversification rate of Cymbidium has decreased since its origin,and is positively associated with changes in temperature and monsoon intensity.Favorable hydrothermal conditions brought by monsoon intensification in the early Miocene possibly contributed to the initial rapid diversification,after which the net diversification rate was reduced with the cooling climate after the middle Miocene.The transition from epiphytic to terrestrial habits may have enabled adaptation to cooler environments and colonization of northern niches,yet without a significant effect on diversification rates.This study provides new insights into how monsoon activity and temperature changes affected the diversification dynamics of plants in Southeast Asia.展开更多
Cymbidium goeringii is an economically important ornamental plant,and flower color is one of the main features of C.goeringii that contributes to its high economic value.To clarify the molecular mechanisms underlying ...Cymbidium goeringii is an economically important ornamental plant,and flower color is one of the main features of C.goeringii that contributes to its high economic value.To clarify the molecular mechanisms underlying the role of anthocyanins in mediating differences in color among varieties,liquid chromatography–tandem mass spectrometry was used to perform anthocyanin-targeted metabolomics of seven C.goeringii varieties,including‘Jin Qian Yuan’(JQY),‘Jin Xiu Qian Yuan’(JXQY),‘Miao Jiang Su Die’(MJSD),‘Qian Ming Su’(QMS),‘Shi Chan’(SC),and‘Yang Ming Su’(YMS),as well as the C.goeringii.We detected 64 anthocyanins,including cyanidins,delphinidins,malvidins,pelargonidins,peonidins,petunidins,procyanidins,and flavonoids.We identified six shared differentially accumulated metabolites(DAMs),including cyanidin-3-O-rutinoside,delphinidin-3-Osophoroside,pelargonidin-3-O-rutinoside,peonidin-3-O-(6-O-malonyl-beta-D-glucoside),peonidin-3-Osophoroside,and chalcone.Most DAMs were enriched in the anthocyanin biosynthesis pathway.Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the differentially expressed metabolites were significantly enriched in the anthocyanin biosynthesis pathway.Analysis of the content of differentially expressed metabolites indicated that peonidin-3-O-(6-O-malonyl-beta-D-glucoside)was the key metabolite underlying color differences among C.goeringii varieties.Procyanidin B2,pelargonidin-3-O-galactoside,and naringenin might also affect the color formation of JQY and QMS,SC,and MJSD,respectively.The results of this study shed light on the metabolic mechanism underlying flower color differences in C.goeringii at the molecular level.Our findings will aid future studies of the mechanism of flower color regulation in C.goeringii and have implications for the breeding of new varieties.展开更多
[Objectives]The paper was to explore the impact of different cultivation substrates on the growth of Cymbidium goeringii.[Methods]The impact of 13 distinct cultivation substrates on the growth of C.goeringii was exami...[Objectives]The paper was to explore the impact of different cultivation substrates on the growth of Cymbidium goeringii.[Methods]The impact of 13 distinct cultivation substrates on the growth of C.goeringii was examined using C.goeringii as the test material.[Results]The combination of burning red clay particles(15%),No.4 pine bark(15%),No.3 pine bark(60%),and perlite(10%),as well as the mixture of burning red clay particles(20%),No.4 pine bark(15%),No.3 pine bark(55%),and perlite(10%),yielded superior results.These formulations resulted in an increased number of new roots in C.goeringii,a reduction in the incidence of decayed roots,and enhancements in the number of tillers,new leaves,and flowers.[Conclusions]The selection of substrates may serve as a valuable reference for the cultivation of C.goeringii.展开更多
The infrageneric classification currently in use for Cymbidium is based on gross morphology, with emphasis on the number of pollinia and state of fusion between lip and column. The sequences of nrDNA regions of 27...The infrageneric classification currently in use for Cymbidium is based on gross morphology, with emphasis on the number of pollinia and state of fusion between lip and column. The sequences of nrDNA regions of 27 species and 3 cultivars of Cymbidium and 3 outgroup species ( Eulophia graminea, Geodorum densiflorum, Amitostigma pinguiculum) were analyzed using PCR amplification and direct DNA sequencing. The phylogenetic trees generated from maximum parsimony analysis, however, show that the existing division among three subgenera (subgen. Cymbidium , subgen. Cyperorchis and subgen. Jensoa ) should be evaluated with more data. Subgenus Cyperorchis was not a monophyletic group, with the unexpected nesting of C. dayanum (subgen. Cymbidium ) within it; subgenus Jensoa also appeared paraphyletic, with C. lancifolium being the sister group to the remainder of the genus; species of subgen. Cymbidium appeared polyphyletic, being split into several clades and intermixed with the main subgen. Cyperorchis and subgen. Jensoa clades, respectively. However, because of the insufficiency of informative characters of ITS sequences, some of the clades identified, especially the major lineages of Cymbidium , received relatively low support; sectional delimitations were also not clear within each subgenus. Further study is needed for achieving a robust phylogeny of Cymbidium .展开更多
Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger...Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger air space and lower water holding capacity in comparison with sphagnum (abbreviated as SP) medium. Leaf growth rate, chlorophyll contents, fresh weight and photosynthetic rate change along with the changes of nitrogen concentrations of both SP and PH/S media. According to the investigation,when the nitrogen concentration was 222 75?mg·L -1 , the photosynthetic rate of SP\|medium reached the highest. The practical nitrogen content was 180 00?mg·L -1 for PH/S medium . The maximum of flower quantity (number per spike) was gotten by treating of N/P 2O 5/K 2O=10/30/20 on PH/S medium, and N/P 2O 5/K 2O=0/23/29 treatment was the lowest on PH/S medium. The differences in small flowers, stem length and flower quantity among the two media and the three fertilization methods reached significant at 0 05 level,respectively.展开更多
[Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid ...[Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro. [Method] With mature seeds of C. dayanum as explants, different media were selected to establish asymbiotic germination technique system. With protocorms as materials, conservation, resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily. [Result] Mature seeds of C. dayanum could germinate after cultured 90 days on MS media as well as ＂Hyponex 1＂ media. The germination rate reached more than 98%. Protocorms inoculated in ＂Hyponex 1＂ media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%. Conserved protocorms could realize resumptive preliferation culture both on 1/2 MS and ＂Hyponex 1＂ media. The seed- ling-strengthening and rooting media were 1/2 MS media. [Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C. dayanum germplasm resource.展开更多
An intermediate between Cymbidium lancifolium Hook. and C.ensifolium (L.) Sw. is described as a new hybrid of them: C.× oblancifolium I. J. Liu et S. C. Chen. It is widespread in southern Sic huan Province in so...An intermediate between Cymbidium lancifolium Hook. and C.ensifolium (L.) Sw. is described as a new hybrid of them: C.× oblancifolium I. J. Liu et S. C. Chen. It is widespread in southern Sic huan Province in southwestern China . After being introduced into cultivation, it remains steady in its character an d indicates remarkable growth. It also exhibits great aesthetic values.展开更多
[Objective] This study was conducted to screen the best fertilization mode for potted Cymbidium sinense ＇Qihei＇. [Method] According to the contents of the N, P and K elements in C. sinense ＇Qihei＇ and the main fer...[Objective] This study was conducted to screen the best fertilization mode for potted Cymbidium sinense ＇Qihei＇. [Method] According to the contents of the N, P and K elements in C. sinense ＇Qihei＇ and the main fertilization modes used by producers and related literature information, the effects of 8 kinds of fertilization models on the growth of C. sinense ＇Qihei＇ were studied. Twenty six morphologic and physiological indexes of C. sinense ＇Qihei＇ growth were measured. [Result] Significant differences were shown on 21 indexes. [Conclusion] Based on the 26 in- dexes, the best fertilization mode included the steps of applying 14-14-14 （N-P20^- K20） slow-release fertilizer particles （8 g per bag） once in April, July and October, respectively; drip-applying 800 times of 30-10-10 water-soluble fertilizer once every half a month from April to September; and drip-applying 800 times of 15-5-30 wa- ter-soluble fertilizer （150 ml） once every half a month from October to December.展开更多
[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA...[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA was extracted from C.faberi Rolfe with method of modified CTAB.Different factors which affected ISSR amplification reaction were optimized.[Result] High-quality genomic DNA was obtained from C.faberi Rolfe.And the optimal reaction system was as follows：25 μl amplification reactions system contained 2.5 μl 10 × PCR buffer,2.0 mmol/L MgCl2,60 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L ISSR primer and 15.85 μl ddH2O.The optimal amplification procedures were pre-denaturing for 5 min at 94 ℃,followed by 40 cycles of denaturing for 30 s at 94 ℃,annealing for 30 s at a temperature of 2-3 ℃ lower than melting temperature of each primer pair,extension for 50 s at 72 ℃.Then extension step of 7 min at 72 ℃ was performed.[Conclusion] The optimal system could provide a favorable basis for further study on genetic diversity of C.faberi Rolfe by using ISSR molecular marker technique.展开更多
基金supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31000000)The 14th Five-Year Plan of the Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences (XTBG-1450101)+3 种基金the Science and Technology Basic Resources Investigation Program of China (2021FY100200)the Key Basic Research Program of Yunnan Province,China (202101BC070003)the Yunnan Revitalization Talent Support Program"Young Talent"and"Innovation Team"ProjectsEcological and Environmental Conservation Program from the Department of Ecology and Environment of Yunnan Province。
文摘Cymbidium(Orchidaceae:Epidendroideae),with around 60 species,is widely-distributed across Southeast Asia,providing a nice system for studying the processes that underlie patterns of biodiversity in the region.However,phylogenetic relationships of Cymbidium have not been well resolved,hampering investigations of species diversification and the biogeographical history of this genus.In this study,we construct a plastome phylogeny of 56 Cymbidium species,with four well-resolved major clades,which provides a framework for biogeographical and diversification rate analyses.Molecular dating and biogeographical analyses show that Cymbidium likely originated in the region spanning northern IndoBurma to the eastern Himalayas during the early Miocene(~21.10 Ma).It then rapidly diversified into four major clades in East Asia within approximately a million years during the middle Miocene.Cymbidium spp.migration to the adjacent regions(Borneo,Philippines,and Sulawesi)primarily occurred during the Pliocene-Pleistocene period.Our analyses indicate that the net diversification rate of Cymbidium has decreased since its origin,and is positively associated with changes in temperature and monsoon intensity.Favorable hydrothermal conditions brought by monsoon intensification in the early Miocene possibly contributed to the initial rapid diversification,after which the net diversification rate was reduced with the cooling climate after the middle Miocene.The transition from epiphytic to terrestrial habits may have enabled adaptation to cooler environments and colonization of northern niches,yet without a significant effect on diversification rates.This study provides new insights into how monsoon activity and temperature changes affected the diversification dynamics of plants in Southeast Asia.
基金supported by the Study on Resource Collection and New Variety Breeding of the Guizhou Mountainous Characteristic Flower C.goeringii(QianKeHe[2022]General 107)the Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China,Guizhou Academy of Forestry.
文摘Cymbidium goeringii is an economically important ornamental plant,and flower color is one of the main features of C.goeringii that contributes to its high economic value.To clarify the molecular mechanisms underlying the role of anthocyanins in mediating differences in color among varieties,liquid chromatography–tandem mass spectrometry was used to perform anthocyanin-targeted metabolomics of seven C.goeringii varieties,including‘Jin Qian Yuan’(JQY),‘Jin Xiu Qian Yuan’(JXQY),‘Miao Jiang Su Die’(MJSD),‘Qian Ming Su’(QMS),‘Shi Chan’(SC),and‘Yang Ming Su’(YMS),as well as the C.goeringii.We detected 64 anthocyanins,including cyanidins,delphinidins,malvidins,pelargonidins,peonidins,petunidins,procyanidins,and flavonoids.We identified six shared differentially accumulated metabolites(DAMs),including cyanidin-3-O-rutinoside,delphinidin-3-Osophoroside,pelargonidin-3-O-rutinoside,peonidin-3-O-(6-O-malonyl-beta-D-glucoside),peonidin-3-Osophoroside,and chalcone.Most DAMs were enriched in the anthocyanin biosynthesis pathway.Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the differentially expressed metabolites were significantly enriched in the anthocyanin biosynthesis pathway.Analysis of the content of differentially expressed metabolites indicated that peonidin-3-O-(6-O-malonyl-beta-D-glucoside)was the key metabolite underlying color differences among C.goeringii varieties.Procyanidin B2,pelargonidin-3-O-galactoside,and naringenin might also affect the color formation of JQY and QMS,SC,and MJSD,respectively.The results of this study shed light on the metabolic mechanism underlying flower color differences in C.goeringii at the molecular level.Our findings will aid future studies of the mechanism of flower color regulation in C.goeringii and have implications for the breeding of new varieties.
基金Supported by Spark Program of Fujian Province(2021S0055).
文摘[Objectives]The paper was to explore the impact of different cultivation substrates on the growth of Cymbidium goeringii.[Methods]The impact of 13 distinct cultivation substrates on the growth of C.goeringii was examined using C.goeringii as the test material.[Results]The combination of burning red clay particles(15%),No.4 pine bark(15%),No.3 pine bark(60%),and perlite(10%),as well as the mixture of burning red clay particles(20%),No.4 pine bark(15%),No.3 pine bark(55%),and perlite(10%),yielded superior results.These formulations resulted in an increased number of new roots in C.goeringii,a reduction in the incidence of decayed roots,and enhancements in the number of tillers,new leaves,and flowers.[Conclusions]The selection of substrates may serve as a valuable reference for the cultivation of C.goeringii.
文摘The infrageneric classification currently in use for Cymbidium is based on gross morphology, with emphasis on the number of pollinia and state of fusion between lip and column. The sequences of nrDNA regions of 27 species and 3 cultivars of Cymbidium and 3 outgroup species ( Eulophia graminea, Geodorum densiflorum, Amitostigma pinguiculum) were analyzed using PCR amplification and direct DNA sequencing. The phylogenetic trees generated from maximum parsimony analysis, however, show that the existing division among three subgenera (subgen. Cymbidium , subgen. Cyperorchis and subgen. Jensoa ) should be evaluated with more data. Subgenus Cyperorchis was not a monophyletic group, with the unexpected nesting of C. dayanum (subgen. Cymbidium ) within it; subgenus Jensoa also appeared paraphyletic, with C. lancifolium being the sister group to the remainder of the genus; species of subgen. Cymbidium appeared polyphyletic, being split into several clades and intermixed with the main subgen. Cyperorchis and subgen. Jensoa clades, respectively. However, because of the insufficiency of informative characters of ITS sequences, some of the clades identified, especially the major lineages of Cymbidium , received relatively low support; sectional delimitations were also not clear within each subgenus. Further study is needed for achieving a robust phylogeny of Cymbidium .
文摘Effects of two media and fertilizer levels on the growth of Cymbidium hybridium were studied. Results demonstrated that peanut\|hull mixed sand (abbreviated as PH/S=1/1) medium had higher total porosity and larger air space and lower water holding capacity in comparison with sphagnum (abbreviated as SP) medium. Leaf growth rate, chlorophyll contents, fresh weight and photosynthetic rate change along with the changes of nitrogen concentrations of both SP and PH/S media. According to the investigation,when the nitrogen concentration was 222 75?mg·L -1 , the photosynthetic rate of SP\|medium reached the highest. The practical nitrogen content was 180 00?mg·L -1 for PH/S medium . The maximum of flower quantity (number per spike) was gotten by treating of N/P 2O 5/K 2O=10/30/20 on PH/S medium, and N/P 2O 5/K 2O=0/23/29 treatment was the lowest on PH/S medium. The differences in small flowers, stem length and flower quantity among the two media and the three fertilization methods reached significant at 0 05 level,respectively.
基金Research supported by national science and technology basic conditions platform program(2005DKA21000-5-63).~~
文摘[Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro. [Method] With mature seeds of C. dayanum as explants, different media were selected to establish asymbiotic germination technique system. With protocorms as materials, conservation, resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily. [Result] Mature seeds of C. dayanum could germinate after cultured 90 days on MS media as well as ＂Hyponex 1＂ media. The germination rate reached more than 98%. Protocorms inoculated in ＂Hyponex 1＂ media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%. Conserved protocorms could realize resumptive preliferation culture both on 1/2 MS and ＂Hyponex 1＂ media. The seed- ling-strengthening and rooting media were 1/2 MS media. [Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C. dayanum germplasm resource.
文摘An intermediate between Cymbidium lancifolium Hook. and C.ensifolium (L.) Sw. is described as a new hybrid of them: C.× oblancifolium I. J. Liu et S. C. Chen. It is widespread in southern Sic huan Province in southwestern China . After being introduced into cultivation, it remains steady in its character an d indicates remarkable growth. It also exhibits great aesthetic values.
基金Supported by Science and Technology Planning Project of Guangdong Province(2012A020602036)
文摘[Objective] This study was conducted to screen the best fertilization mode for potted Cymbidium sinense ＇Qihei＇. [Method] According to the contents of the N, P and K elements in C. sinense ＇Qihei＇ and the main fertilization modes used by producers and related literature information, the effects of 8 kinds of fertilization models on the growth of C. sinense ＇Qihei＇ were studied. Twenty six morphologic and physiological indexes of C. sinense ＇Qihei＇ growth were measured. [Result] Significant differences were shown on 21 indexes. [Conclusion] Based on the 26 in- dexes, the best fertilization mode included the steps of applying 14-14-14 （N-P20^- K20） slow-release fertilizer particles （8 g per bag） once in April, July and October, respectively; drip-applying 800 times of 30-10-10 water-soluble fertilizer once every half a month from April to September; and drip-applying 800 times of 15-5-30 wa- ter-soluble fertilizer （150 ml） once every half a month from October to December.
基金Supported by a Program from the State Environmental Protection Administration(20061A0013)~~
文摘[Objective] By using the genomic DNA of Cymbidium faberi Rolfe as template,the factors that affect the result of ISSR-PCR reaction system were researched and the optimal system was established.[Method] The genomic DNA was extracted from C.faberi Rolfe with method of modified CTAB.Different factors which affected ISSR amplification reaction were optimized.[Result] High-quality genomic DNA was obtained from C.faberi Rolfe.And the optimal reaction system was as follows：25 μl amplification reactions system contained 2.5 μl 10 × PCR buffer,2.0 mmol/L MgCl2,60 ng template DNA,160 μmol/L dNTPs,1.25 U Taq DNA polymerase,0.4 μmol/L ISSR primer and 15.85 μl ddH2O.The optimal amplification procedures were pre-denaturing for 5 min at 94 ℃,followed by 40 cycles of denaturing for 30 s at 94 ℃,annealing for 30 s at a temperature of 2-3 ℃ lower than melting temperature of each primer pair,extension for 50 s at 72 ℃.Then extension step of 7 min at 72 ℃ was performed.[Conclusion] The optimal system could provide a favorable basis for further study on genetic diversity of C.faberi Rolfe by using ISSR molecular marker technique.
