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Recombinant adeno-associated virus 8-mediated inhibition of microRNA let-7a ameliorates sclerosing cholangitis in a clinically relevant mouse model
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作者 Hui Hua Qian-Qian Zhao +9 位作者 Miriam Nkesichi Kalagbor Guo-Zhi Yu Man Liu Zheng-Rui Bian Bei-Bei Zhang Qian Yu Yin-Hai Xu Ren-Xian Tang Kui-Yang Zheng Chao Yan 《World Journal of Gastroenterology》 SCIE CAS 2024年第5期471-484,共14页
BACKGROUND Primary sclerosing cholangitis(PSC)is characterized by chronic inflammation and it predisposes to cholangiocarcinoma due to lack of effective treatment options.Recombinant adeno-associated virus(rAAV)provid... BACKGROUND Primary sclerosing cholangitis(PSC)is characterized by chronic inflammation and it predisposes to cholangiocarcinoma due to lack of effective treatment options.Recombinant adeno-associated virus(rAAV)provides a promising platform for gene therapy on such kinds of diseases.A microRNA(miRNA)let-7a has been reported to be associated with the progress of PSC but the potential therapeutic implication of inhibition of let-7a on PSC has not been evaluated.AIM To investigate the therapeutic effects of inhibition of a miRNA let-7a transferred by recombinant adeno-associated virus 8(rAAV8)on a xenobiotic-induced mouse model of sclerosing cholangitis.METHODS A xenobiotic-induced mouse model of sclerosing cholangitis was induced by 0.1% 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine(DDC)feeding for 2 wk or 6 wk.A single dose of rAAV8-mediated anti-let-7a-5p sponges or scramble control was injected in vivo into mice onset of DDC feeding.Upon sacrifice,the liver and the serum were collected from each mouse.The hepatobiliary injuries,hepatic inflammation and fibrosis were evaluated.The targets of let-7a-5p and downstream molecule NF-κB were detected using Western blot.RESULTS rAAV8-mediated anti-let-7a-5p sponges can depress the expression of let-7a-5p in mice after DDC feeding for 2 wk or 6 wk.The reduced expression of let-7a-5p can alleviate hepato-biliary injuries indicated by serum markers,and prevent the proliferation of cholangiocytes and biliary fibrosis.Furthermore,inhibition of let-7a mediated by rAAV8 can increase the expression of potential target molecules such as suppressor of cytokine signaling 1 and Dectin1,which consequently inhibit of NF-κB-mediated hepatic inflammation.CONCLUSION Our study demonstrates that a rAAV8 vector designed for liver-specific inhibition of let-7a-5p can potently ameliorate symptoms in a xenobiotic-induced mouse model of sclerosing cholangitis,which provides a possible clinical translation of PSC of human. 展开更多
关键词 Primary sclerosing cholangitis Recombinant adeno-associated virus 8 Let-7a-5p Therapeutic effects INFLAMMATION
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Effect of Curcumin on Influenza Virus HIN1 and H3N2 in Vitro 被引量:15
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作者 刘妮 孟以蓉 +2 位作者 张俊丽 朱宇同 黄正昌 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2008年第9期534-535,共2页
Objective:To investigate the inhibitory effect of curcumin on influenza virus HIN1 and H3N2 in vitro, Methods:The directly killing role of cureumin extract in vitro to influenza virus type A subtype H1N1 and H3N2 wa... Objective:To investigate the inhibitory effect of curcumin on influenza virus HIN1 and H3N2 in vitro, Methods:The directly killing role of cureumin extract in vitro to influenza virus type A subtype H1N1 and H3N2 was evaluated by the canine kidney cells (MDCK), Results:The largest non toxic concentration of curcumin extract was 12, 5g/L and the effective inhibitory concentration to H1N1 and H3N2 was 6, 25G/1 AND 1,56g/L respectively, Conclusion: Curcumin extract have directly killing effect on H1N1 and H3N2 infections. 展开更多
关键词 CURCUMIN influenza virus directly killing effect
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Control Effect of Bio-guide Wasp Virus Missile on Ostrinia furnacalis and Its Benefits for Maize Production in Nanchong City
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作者 彭昌家 白体坤 +8 位作者 丁攀 冯礼斌 尹怀中 龙维国 杨宇衡 杨建华 何海燕 苟建华 罗怀海 《Agricultural Science & Technology》 CAS 2016年第3期662-666,共5页
In order to promote "green plant protection", reduce the administration dosage and residue of pesticides and control cost of environment pollution, and improve maize production, quality and agricultural ecological e... In order to promote "green plant protection", reduce the administration dosage and residue of pesticides and control cost of environment pollution, and improve maize production, quality and agricultural ecological environment safety. In this study, the control effect and benefit of bio-guide wasp virus missile(BGWVM) on Ostrinia furnacalis in Nanchong City was investigated by using insect light traps and combined method of field investigation and experimental demonstration. According to the results, applying 30-37 pairs of BGWVM per hectare exhibited an average control efficiency of 78.0%, and the control cost was 139.50-172.50 CNY/hm2, which was reduced by 142.50-168.00 CNY/hm^2 compared with conventional chemical control. Average maize yield in BGWVM demonstration plot was 817.8 kg/hm2 higher and improved by 11.0% compared with blank control plot. The output value was improved by 1 799.16 CNY/hm^2 and the revenue increased by 1 941.66-1 967.16CNY/hm2. During 2003-2014, the cumulative demonstration and popularization area of BGWVM technique in Nanchong City had reached 24 507.33 hm^2, and the cumulative yield increment reached 20.042 1 million kg. BGWVM application improved total output value by 44.092 6 million CNY and saved control cost by 3.492 3-4.117 2 million CNY, thus increasing the revenue by 47.584 9-48.209 8 million CNY. BGWVM application reduced 551.41 t of 5% dimehypo GG or 12.87 t of 90%monosultap WP. In addition, BGWVM technique is simple to use, labor-saving and safe for human, animals, environment and crops, with significant economic, ecological and social benefits and without any side effect, which has become an ideal substitute for controlling O. furnacalis and reducing chemical pesticides in organic agriculture industry, modern agriculture industry, production of green and pollution-free agricultural products and IBM prevention and control demonstration gardens. 展开更多
关键词 Ostrinia furnacalis Bio-guide wasp virus missile Prevention and control effect BENEFIT Evaluation
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Antiviral Effect of Emodin from Rheum palmatum against Coxsakievirus B_5 and Human Respiratory Syncytial Virus In Vitro 被引量:8
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作者 刘钊 马年 +1 位作者 钟研 杨占秋 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2015年第6期916-922,共7页
Summary: Viral infections are the major causes of morbidity and mortality in elderly people and young children throughout the world. The most common pathogens include coxsackie virus (CV) and respira- tory syncytia... Summary: Viral infections are the major causes of morbidity and mortality in elderly people and young children throughout the world. The most common pathogens include coxsackie virus (CV) and respira- tory syncytial virus (RSV). However, no antiviral agents with low toxicity and drug resistance are cur- rently available in clinic therapy. The present study aimed to examine the antiviral activities of emodin (an ingredient of Rheum palmatum) against CVB5 and RSV infections, in an attempt to discover new antiviral agents for virus infection. The monomer emodin was extracted and isolated from Rheum pal- matum. The antiviral activities of emodin on HEp-2 cells were evaluated, including virus replication in- hibition, virucidal and anti-absorption effects, by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tet- razolium bromide (MTT) assay and plaque reduction assay (PRA). The kinetics of virus inhibition by emodin in a period of 14 h was further determined by plaque assay and quantitative real time PCR (qPCR). Cytokine (IFN-γ, TNF-α) mRNA expressions after emodin treatment (7.5, 15, 30 μmol/L) were also assessed by qPCR post-infection. The results showed that emodin had potent inhibitory activities against CVB5 and RSV, with the 50% effective concentration (EC50) ranging from 13.06 to 14.27 μmol/L and selectivity index (SI) being 5.38-6.41 μmol/L. However, emodin couldn't directly inacti- vate the viruses or block their absorption to cells. It acted as a biological synthesis inhibitor against CVB4 and RSV in a concentration- and time-dependent manner, especially during the first 0-4 h post-infection. Moreover, emodin could decrease the mRNA expression of IFN-α but enhance TNF-γ expression significantly compared to the viral controls in vitro. Our results provide a molecular basis for development of emodin as a novel and safe antiviral agent for human enterovirus and respiratory virus infection in the clinical therapy. 展开更多
关键词 EMODIN antiviral effect coxsakievirus B5 respiratory syncytial virus
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Effects of Biological Pesticides on Prevention and Treatment of Virus Diseases in Greenhouse Tomato in Autumn 被引量:1
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作者 彭昌家 白体坤 +2 位作者 丁攀 冯礼斌 杨宇衡 《Agricultural Science & Technology》 CAS 2016年第8期1921-1925,共5页
To alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, forecasting, randomized block design and statistical analysis were adopted to c... To alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, forecasting, randomized block design and statistical analysis were adopted to conduct field efficacy tests for Lentinan + Gibberellin-heteroauxin-brassinolide (GHB) and Ningnanmycin + GHB. The results showed that when the virus diseases in greenhouse tomato were serious, 0.5% Lentinan AS 3 000 ml + GHB WP 600 g and 8% Ningnanmycin AS 900 ml + GHB WP 600 g per hectare were applied 4 times with an interval of 7 days, and the control effects on the 10th, 17th, 26th and 35th day were above 88%, 87%, 78% and 67%, respectively. The difference in control effect of the two biological pesticides was insignificant, while their control effects were all significantly better than that of moroxydine hydrochloride (CK) + GHB. The obtained results indicated that Lentinan + GHB and Ningnanmycin + GHB were the ideal biological pesticides, which could be used not only for controlling tomato virus diseases, but also for realizing modern, pollution-free, green and organic agricultural production. 展开更多
关键词 Tomato virus disease Biological pesticide LENTINAN NINGNANMYCIN Gibberellin-heteroauxin-brassinolide Control effect
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Tea Polyphenols Exerts Anti-hepatitis B Virus Effects in a Stably HBV-transfected Cell Line 被引量:4
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作者 叶翩 张淑玲 +4 位作者 赵雷 董继华 揭盛华 庞然 李淑莉 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2009年第2期169-172,共4页
In this study, the anti-HBV effects of tea polyphenols (TP) were examined. After cells were exposed to TP for 3, 6, 9 days, amounts of HBsAg, HBeAg and HBV-DNA released into the supernatant of the cultured HepG2 2.2... In this study, the anti-HBV effects of tea polyphenols (TP) were examined. After cells were exposed to TP for 3, 6, 9 days, amounts of HBsAg, HBeAg and HBV-DNA released into the supernatant of the cultured HepG2 2.2.15 cells were detected. TP, to some extent, inhibited the secretion of HBsAg and strongly suppressed the secretion of HBeAg in a dose-dependent (P〈0.01) and time-dependent manner, with 50% maximal inhibitory concentration (IC50) value being 7.34μg/mL on the 9th day, but the time-dependence was not significant (P=0.051). Expression of HBV-DNA in the supernatant of the cell culture also was significantly decreased in a dose-dependent fashion (P〈0.01). The ICS0 of TP in inhibiting HBV DNA was 2.54 pg/mL. It concluded that TP possessed potential anti-HBV effects and may be used as a treatment alternative for HBV infection. 展开更多
关键词 liver diseases hepatitis B virus anti-HBV effect tea polyphenols (TP) HepG2 2.2.15
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Anti-tumor Effects of a Recombinant Fowlpox Virus Expressing Apoptin on Human Cervical Carcinoma in Vivo and in Vitro 被引量:3
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作者 ZHU Ji-hong LI Xiao +7 位作者 SUN Li-li ZHANG Mu-chun KAN Shi-fu LIU Lei HUANG Hai-yan YANG Guo-hua PIAO Bing-guo JIN Ning-yi 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2011年第4期646-650,共5页
Apoptin is a chicken anemia virus-derived,p53-independent,bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis of various human tumor cells,but not of normal diploid cells.To explore t... Apoptin is a chicken anemia virus-derived,p53-independent,bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis of various human tumor cells,but not of normal diploid cells.To explore the application of apoptin in tumor gene therapy,we used a recombinant fowlpox virus expressing apoptin protein (vFV-Apoptin) to investigate the anti-tumor effectes of vFV-Apoptin on human cervical carcinoma(HeLa) cells in vivo and in vitro through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide(MTT) assay,acridine orage/ethidium bromide(AO/EB) and annexin V staining test,respectively.The results show that vFV-Apoptin inhibites the proliferation of HeLa cells in vitro through inducing the apoptosis of HeLa cells,and the inhibition effect of vFV-Apoptin has a dose-effect and time-effect relationship.The results of animal models show that vFV-Apoptin significantly inhibits tumor growth,extends the lifespan of animals and improves the mean survival.Experimental results indicate that vFV-Apoptin has a potential application in the tumor gene therapy. 展开更多
关键词 Apoptin gene Chicken anemia virus Human cervical carcinoma Anti-tumor effect
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Antiviral Effects of Compound Traditional Chinese Medicine on Newcastle Disease Virus 被引量:2
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作者 LIU Qun DUAN Liang-liang JIANG Ping-kang 《Animal Husbandry and Feed Science》 CAS 2010年第4期29-31,共3页
[Objective] To investigate the mechanism of compound traditional Chinese medicine (TCM) on Newcastle disease virus (NDV) and to provide a scientific basis for the reasonable usage of antiviral drugs in clinic. [Me... [Objective] To investigate the mechanism of compound traditional Chinese medicine (TCM) on Newcastle disease virus (NDV) and to provide a scientific basis for the reasonable usage of antiviral drugs in clinic. [Method] The compound TCM was composed of Hedyotis diffusa, Lonicera japonica Thunb, Radix astragali and Glycyrrhiza uralensis. Different dilutions of fluid extract were prepared. Its antiviral effects on NDV were observed through three inoculation ways, first, inoculation with the medicine and NDV mixture which had been incubated at 37 ℃; second, incubating chicken embryo fibroblasts (CEF) with the medicine followed by inoculation with NDV; third, inoculation with N DV followed by incubating CEF with the medicine. The A,= was determined by M]-r [ 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) ~ method. Therapeutic indexes were used to evaluate the antiviral effects. [ Result] The minimum effective concentration of the compound TCM which acted through the three ways was 1.0 × 2^-10 1.0 × 2^-8 and 1.0 × 2^-7 g/ml, respectively. The antiviral effects of the compound TCM were the best through inoculation with the incubated medicine and NDV mixture, followed by the second method and the third method. [ Conclusion] The compound TCM can not only kill NDV directly in vitro but also inhibit viral propagation. 展开更多
关键词 Compound traditional Chinese medicine Antiviral effects Chicken embryo fibroblasts Newcastle disease virus
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PLASMID VACCINE ENCODING HN GENE FROM NEWCASTLE DISEASE VIRUS HAS MARKED ANTITUMORAL EFFECT IN VITRO
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作者 薛立娟 金宁一 +2 位作者 龚伟 王宏伟 李萍 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2003年第3期161-166,共6页
Objective: To explore the antitumor effects of hemaagglutinin-neuraminase gene (HN gene) from Newcastle disease virus. Methods: Plasmid vaccine of pIRHN was constructed and transfected into HeLa cells. The expression... Objective: To explore the antitumor effects of hemaagglutinin-neuraminase gene (HN gene) from Newcastle disease virus. Methods: Plasmid vaccine of pIRHN was constructed and transfected into HeLa cells. The expression of HN was analyzed by Western blot analysis, and the mode of cell death was detected by fluorescence microscope, gel electrophoresis and TUNEL assay and the expression of p53 and bcl-2 was also analyzed in transfected Hela cells. The effect of pIRHN on sialic acid contents in the Hela cell was examined. Results: pIRHN nucleic acid vaccines could be expressed in eukaryotic cell. pIRHN could induce apoptosis after HeLa cells were transfected. The effect of antitumor responses of pIRHN was correlated with the contents of sialic acid in tumor cells, and there was no prominent evidence for the relatedness of the antitumor effect with the expression of p53 and bcl-2. Conclusion: pIRHN may become a new antitumor biological agent. 展开更多
关键词 Newcastle disease virus Hemaagglutinin- neuraminase gene Western blot Antitumor effect Nucleic acid vaccines Apoptosis
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The Defense Effect of ABA against Turnip crinkle virus (TCV)
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作者 Hui YANG Ji HUANG +1 位作者 Dongbo MIN Aibo YANG 《Plant Diseases and Pests》 CAS 2012年第2期54-56,共3页
[ Objective ] The paper was to understand whether abscisic acid (ABA) participates the defense effect of hosts against Turnip crinkle virus (TCV). [ Method] By external application of ABA and using the correspondi... [ Objective ] The paper was to understand whether abscisic acid (ABA) participates the defense effect of hosts against Turnip crinkle virus (TCV). [ Method] By external application of ABA and using the corresponding mutants, TCV coat protein levels of various plants were detected by western-blot, and the expression of defense-related genes was analyzed by RT-PCR. [Result] After external application of SA, JA, ET and ABA, only SA and ABA could significantly inhibit TCV accumulation. In arabidopsis-TCV interaction system, SA and ABA had antagonistic effect with each other, the defense effect against TCV induced by ABA was independent on SA pathway. [ Conclusion] The plants could activate distinct signal pathways to adjust the defense of hosts against virus. 展开更多
关键词 Turnip crinkle virus Defense effect Abscisic acid China
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Expression of Porcine Interleukin-2 and Porcine Interleukin-6 and Their Adjuvant Effects on Gene Deleted Vaccine of Pseudorabies Virus(TK^-/gG^-/LacZ^+)
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作者 YAN Lin, HE Qi-gai, CHEN Huan-chun, XIAO Shao-bo, WU Mei-zhou,LU Jian-qiang and HAN Li(Laboratory of Virology, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University , Wuhan 430070 , P. R. China) 《Agricultural Sciences in China》 CAS CSCD 2003年第8期924-929,共6页
Porcine interleukin-2 and porcine interleukin-6 cDNA sequences were cloned into the expressing vectors pET-28a and pGEX-KG respectively. They were expressed in E. coli BL21(DE3)with high-level production. The gene del... Porcine interleukin-2 and porcine interleukin-6 cDNA sequences were cloned into the expressing vectors pET-28a and pGEX-KG respectively. They were expressed in E. coli BL21(DE3)with high-level production. The gene deleted vaccine of pseudorabies virus Ea strain(TK-/gG-/LacZ+)was mixed with the two different purified recombinant proteins each, or both, with the doses of 2, 5 or 10 μg ml-1. Ten groups of pseudorabies negative antibody swines were immuned twice with tested vaccines with different doses, or control vaccine, respectively. The antibody liters of the test groups were detected by neutralization test, and the daily weight gains of swines were calculated and analyzed statistically. In the study, all the neutralizing antibody ti-ters in test groups were higher than the control group, and the recombinant proteins appeared a dose dependent adjuvant effect. The tested vaccines with 2 μg ml-1 pIL-2 and with 10 μg ml-1 pIL-2/pIL-6 got significant and extremely significant differences, compared with the vaccines without pILs. The difference of the daily weight gain indicated the potential positive influence of pIL-2 and pIL-6 on immune protection. 展开更多
关键词 Porcine interleukin-2 Porcine interleukin-6 Expression Gene deleted vaccine of pseudora-bies virus(TK-/gG-/LacZ+) Adjuvant effect
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Control Effects of Different Agents on Tobacco Mosaic Virus Disease
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作者 Weiwei OUYANG Zhengyang ZHANG +9 位作者 Qiuzan ZHONG Changyou SHEN Runsheng LIU Xianyi XIAO Qinggen YANG Wenping RAO Yi LIU Chenggen FAN Hai LIAN Lifang XIE 《Agricultural Biotechnology》 CAS 2019年第6期27-29,63,共4页
[Objectives] This study was conducted to screen out suitable agents for controlling tobacco mosaic virus disease and the best control period in Zhangzhou tobacco area, providing a theoretical basis for the control of ... [Objectives] This study was conducted to screen out suitable agents for controlling tobacco mosaic virus disease and the best control period in Zhangzhou tobacco area, providing a theoretical basis for the control of virus diseases, thereby improving the quality of flue-cured tobacco and the income of tobacco farmers. [Methods] The effects on tobacco mosaic virus disease under the interaction between different agents and different application periods were investigated. The incidence of tobacco mosaic virus disease was investigated, and its control effect was analyzed. [Results] Different agents and different application periods had different control effects on tobacco mosaic virus disease. The incidence of tobacco mosaic virus disease: At 30 and 45 d after transplanting, the incidences of A2B1 treatment were the lowest, at 0.85%, 1.71%, respectively;and at 60 d after transplanting, the incidence of A3B1 treatment was the lowest, only 10.68%. The control effect: At 30 and 45 d after transplanting, A2B1 treatment had better control effects, reaching 79.39% and 73.06%, respectively. [Conclusions] 3% hypersensitive protein sprayed at 1 d before transplanting and 7 and 15 d after transplanting achieved the best effect, followed by 10% ningnanmycin sprayed at 1 d before transplanting and 7 and 15 d after transplanting. In tobacco production, it is recommended to apply 1 000 times dilution of 3% supersensitive protein microgranules for three times(at 1 d before transplanting and 7 and 15 d after transplanting), which can effectively prevent tobacco mosaic virus disease. 展开更多
关键词 Tobacco mosaic virus disease Control effect Hypersensitive protein Oligosaccharins-plant activation protein
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Four FDA-approved drugs exhibited inhibition effect on severe fever with thrombocytopenia syndrome virus in vitro
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作者 WANG Tian-tian YIN Zhi-yun +11 位作者 DENG Ya-li ZHU Qiong ZHOU Min HU Si-jing Wu Qiao-li JIN Jia-yin ZHANG Dan-na LIU Xi-jia JIANG Bo-yong SHEN Shu DENG Fei SHI Jun-ming 《Journal of Hainan Medical University》 2022年第23期11-17,共7页
Objective:To screen the anti-SFTSV drugs from 1430 FDA-approved drugs via mini-genome system,and to investigate which stage of the infection process could be suppressed by the identified drugs.Methods:The SFTSV mini-g... Objective:To screen the anti-SFTSV drugs from 1430 FDA-approved drugs via mini-genome system,and to investigate which stage of the infection process could be suppressed by the identified drugs.Methods:The SFTSV mini-genome system was used to screen drugs with inhibitory effect on SFTSV replication and transcription,and the 50%inhibitory concentration(IC_(50))of each drug was calculated by drug concentration gradient inhibition experiment.Drugs were used to pre-incubate with virus and then incubate with cells,to incubate with virus and cells simultaneously,to incubate with cells after virus invading into cells,or to incubate during the whole infection process,and then qRT-PCR was used to measure the viral RNA copies in the culture supernatant.These experiments were performed to quantitatively determine the inhibition effects of drugs on SFTSV indifferent stages of the whole process including virion stability,entry and post-entry stages,so as to clarify the inhibition mechanism of these drugs.Results:Four drugs including Mycophenolate mofetil,Mycophenolic acid,Nitazoxanide,and Vidofludimus were identified having efficient inhibitory effects on SFTSV RNA replication via minigenome system,with the IC_(50) of 0.014μmol/L,0.627μmol/L,1.283μmol/L,and 0.059μmol/L,respectively.All four drugs showed effective inhibition when adding during the whole SFTSV infection process as well as the post-entry stage.Conclusion:Mycophenolate mofetil,Mycophenolic acid,Nitazoxanide and Vidofludimus show efficient anti-viral effects on SFTSV infection. 展开更多
关键词 Severe fever with thrombocytopenia syndrome virus Drug screening Antiviral effect Inhibition phase
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Effects of Early Infection of Tobacco Mosaic Virus on Photosynthetic Proteins and Its Control
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作者 Xiaojing XU Kunfeng SONG +3 位作者 Fengsheng HAO Jiajia ZHAO Hongxiang GUO Weiqun LIU 《Agricultural Biotechnology》 CAS 2019年第2期5-9,共5页
[Objectives] This study was conducted to explore the optimal period of TMV control and the physiological sites that interfere with TMV infection. [Methods] Proteome analysis was performed on the host tissues(tobacco p... [Objectives] This study was conducted to explore the optimal period of TMV control and the physiological sites that interfere with TMV infection. [Methods] Proteome analysis was performed on the host tissues(tobacco plants) at different time periods of viral infection, to verify the changes in the expression of differential protein genes and N and PR1-a in the photosynthetic pathway and porphyrin metabolism and chlorophyll metabolism pathways in proteome; and the tobacco plants were treated with different preparations. [Results] The expression levels of N and PR1-a in the tobacco leaves treated with preparation B reached the highest level, and the effects on the expression levels of the differential protein genes were also the most significant. The control effects of the preparations were tested by the half-leaf method, and the results showed that preparation B had a significant control effect against the early infection of the virus.[Conclusions] This study lays a foundation for the prevention and control of tobacco mosaic virus on crops. 展开更多
关键词 TOBACCO MOSAIC virus PROTEOME PHOTOSYNTHETIC system CONTROL effect
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Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo 被引量:49
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作者 Sanpha Kallon Xiaorong Li +7 位作者 Jun Ji Cuiying Chen Qianyun Xi Shuang Chang Chunyi Xue Jingyun Ma Qingmei Xie Youngliang Zhang 《Journal of Animal Science and Biotechnology》 SCIE CAS 2013年第4期325-335,共11页
This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated... This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated by an Mqq- [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MFIC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to H9N2 AIV wet enhanced in the first week after APS treatment. The results indicated that APS treatment reduces H9N2 AIV replication and promotes early humoral immune responses in young chickens.This study investigated the humoral immunization of Astragalus polysaccharide (APS) against HgN2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on HgN2 infection was evaluated by an M]q- [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MHC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to PIgN2 AIV were enhanced in the first week after APS treatment. The results indicated that APS treatment reduces HgN2 AIV replication and promotes early humoral immune responses in young chickens. 展开更多
关键词 Astrogalus polysaccharide HgN2 avian influenza virus Immune effect
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Role of hepatitis B virus DNA integration in human hepatocarcinogenesis 被引量:25
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作者 Hoang Hai Akihiro Tamori Norifumi Kawada 《World Journal of Gastroenterology》 SCIE CAS 2014年第20期6236-6243,共8页
Liver cancer ranks sixth in cancer incidence, and is the third leading cause of cancer-related deaths worldwide. Hepatocellular carcinoma (HCC) is the most common type of liver cancer, which arises from hepatocytes an... Liver cancer ranks sixth in cancer incidence, and is the third leading cause of cancer-related deaths worldwide. Hepatocellular carcinoma (HCC) is the most common type of liver cancer, which arises from hepatocytes and accounts for approximately 70%-85% of cases. Hepatitis B virus (HBV) frequently causes liver inflammation, hepatic damage and subsequent cirrhosis. Integrated viral DNA is found in 85%-90% of HBV-related HCCs. Its presence in tumors from non-cirrhotic livers of children or young adults further supports the role of viral DNA integration in hepatocarcinogenesis. Integration of subgenomic HBV DNA fragments into different locations within the host DNA is a significant feature of chronic HBV infection. Integration has two potential consequences: (1) the host genome becomes altered (&#x0201c;cis&#x0201d; effect); and (2) the HBV genome becomes altered (&#x0201c;trans&#x0201d; effect). The cis effect includes insertional mutagenesis, which can potentially disrupt host gene function or alter host gene regulation. Tumor progression is frequently associated with rearrangement and partial gain or loss of both viral and host sequences. However, the role of integrated HBV DNA in hepatocarcinogenesis remains controversial. Modern technology has provided a new paradigm to further our understanding of disease mechanisms. This review summarizes the role of HBV DNA integration in human carcinogenesis. 展开更多
关键词 Hepatitis B virus INTEGRATION HEPATOCARCINOGENESIS Cis effect Trans effect Whole genome sequencing
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Preparation and immunoprotective effects of a virus-like particle candidate vaccine of the dominant epidemic D3 genotype coxsackievirus A6 in China
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作者 Xiaoliang Li Xizhu Xu +9 位作者 Jichen Li Huanhuan Lu Congcong Wang Rui Wang Jinbo Xiao Ying Liu Yang Song Jingdong Song Qiang Sun Yong Zhang 《Biosafety and Health》 CAS CSCD 2024年第1期28-34,共7页
Coxsackievirus A6 of the D3a genotype(CVA6 D3a)is a primary pathogen causingmainland of China's hand,foot,and mouth disease(HFMD).Viral‐like particle(VLP)vaccines represent a potential candidate vaccine to preven... Coxsackievirus A6 of the D3a genotype(CVA6 D3a)is a primary pathogen causingmainland of China's hand,foot,and mouth disease(HFMD).Viral‐like particle(VLP)vaccines represent a potential candidate vaccine to prevent HFMD.This study collected Anti‐CVA6 D3a VLPs serum from BALB/c female mice immunized using CVA6 D3a VLPs.The neutralizing antibody levels were compared against the representative 14‐JX2018(D3a)and N4‐YN2015(D3b)strains between the antisera of different immune pathways.The immunoprotective effect of anti‐CVA6 D3a VLPs against these strains was monitored using pathological sections and immuno-histochemical results of lung and skeletal muscle tissues in seven‐day‐old Institute of Cancer Research(ICR)mice.Immunological protection against different branches of viruses was evaluated in 7‐day‐old(serum passive immune protection)and 14‐day‐old(VLPs active immune protection)neonatal ICR mice models.Serum‐neutralizing antibody levels were positively correlated with the number of immunizations and higher against 14‐JX2018 than against N4‐YN2015.Furthermore,these levels were significantly higher with abdominal injection than intramuscular injection.The immunized serum of 7‐day‐old ICR mice inoculated three times was 100%protected against CVA6 D3a 14‐JX2018(lethal titer:106.25 TCID 50)and CVA6 D3b N4‐YN2015(lethal titer:105.25TCID 50)fatal attacks,respectively.For ICR mice that have completed two active immunizations for 14 days,both CVA6 D3a 14‐JX2015(challenge titer:108.25 TCID 50)and CVA6 D3b N4‐YN2015(challenge titer:107.25 TCID 50)were used for the challenge,and the mice were able to survive.Overall,the CVA6 D3a VLPs prepared in this study are a potential vaccine candidate for CVA6,as it has the optimal protective effect against both CVA6 D3a and D3b evolutionary branches viruses. 展开更多
关键词 Coxsackievirus A6 D3 genotype virus‐like particle Immunoprotective effect
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Cryptoporic acid E from Cryptoporusvolvatus inhibits influenza virus replication in vitro
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作者 GAO Li HAN Jia-yuan +2 位作者 WANG Jun-chi SI Jian-yong CAO Li 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2016年第10期1091-1092,共2页
Influenza virus infection is a global public health issue.The effectiveness of antiviral agents for influenza has been limited by the emergence of drugresistant virus strains.Therefore,there is an urgent need to ident... Influenza virus infection is a global public health issue.The effectiveness of antiviral agents for influenza has been limited by the emergence of drugresistant virus strains.Therefore,there is an urgent need to identify novel antiviral therapies.Our previous studies have found that Cryptoporus volvatus extract could potently inhibit influenza virus replication in vitro and in vivo.However,the effective component of Cryptoporus volvatus which mediated the antiviral activity hasn′t been identified.Here,we identified a novel anti-influenza molecule,cryptoporic acid E(CAE),from Cryptoporus volvatus.Our results showed that CAE had broad-spectrum anti-influenza activity against 2009 pandemic strain A/Beijing/07/2009(H1N1/09),seasonal strain A/Jiangxi/262/05(H3N2),mouse adapted strains A/WSN/33(H1N1)and A/PR8/34(H1N1).We further investigated the mode of CAE action,and found that CAE directlyattenuated influenza virus infectivity.Time-course-analysis indicated that CAE exerted its inhibition mainly at middle stage of the replication cycle of influenza virus.Subsequently,we confirmed that CAE blocked virus RNA replication and transcription in MDCK cells and CAE repressed influenza virus RNA polymerase activity.In addition,we found that CAE impaired influenza virus infectivity by directly targeting virus particles.Our data suggest that CAE is a major effective component of Cryptoporus volvatus and might be a potential candidate for the development of a new anti-influenza virus therapy. 展开更多
关键词 cryptoporic acid E anti-influenza virus effective component in vitro
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Isolation and Identification of Porcine Epidemic Diarrhea Virus(PEDV) HLJ Strain with IPEC-J2 Cells and Phylogenetic Analysis of Its S Gene
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作者 Feng Rui Liu Hai-xin +4 位作者 Zhong Ming Li Xun-liang Huang Xiao-dan Ren Yu-dong Li Guang-xing 《Journal of Northeast Agricultural University(English Edition)》 CAS 2019年第4期63-72,共10页
Porcine epidemic diarrhea(PED)is caused by porcine epidemic diarrhea virus(PEDV),and is characterized by vomiting,diarrhea and dehydration of suckling pigs from 80% to 100% morbidity and 50% to 90% mortality,and resul... Porcine epidemic diarrhea(PED)is caused by porcine epidemic diarrhea virus(PEDV),and is characterized by vomiting,diarrhea and dehydration of suckling pigs from 80% to 100% morbidity and 50% to 90% mortality,and resulted in tremendous economic losses to swine industry.The PEDV mainly infects small intestine of pigs,resulting in vacuolar degeneration and necrosis of mucosal epithelium.The IPEC-J2 is a pig intestine epithelial cell line,which is similar to the intestinal environment of piglets,can be used to isolate and identify the PEDV field isolates.In this study,it appeared the PEDV typical postmortem changes and histopathological lesion of degeneration and destruction of small intestine in infected piglets,and IHC identified that the PEDV distributed in the mucosa and submucosa of small intestine mostly.Furthermore,the PEDV HLJ strain was successfully isolated and characterized in the IPEC-J2 cells,and indicated that the IPEC-J2 cell line was sensitive to isolate and adapt the PEDV field strain,and could be utilized to multiply the PEDV rapidly.The S gene analysis indicated that the PEDV HLJ strain was the prevailed virus,belonged to Group 1 with attenuated virulent DR13,SC1402 and J-S2/2015 strains isolated in South Korea and China from 2014 to 2015.This study had important theoretical and practical significances on analyzing genetic variation of the PEDV,understanding the pathogenic characteristics of the virus and developing new vaccines for the PED. 展开更多
关键词 porcine epidemic diarrhea virus cytopathic effect IPE-J2 cell isolation and identification phylogenetic analysis
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Viricidal activity of several disinfectants against African swine fever virus
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作者 JIANG Cheng-gang SUN Ying +7 位作者 ZHANG Fan AI Xin FENG Xiao-ning HU Wei ZHANG Xian-feng ZHAO Dong-ming BU Zhi-gao HE Xi-jun 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第11期3084-3088,共5页
Prevention of African swine fever,a disease caused by African swine fever virus(ASFV),requires maintenance of high biosecurity standards,which principally relies on disinfection.Finding the perfect disinfectant agains... Prevention of African swine fever,a disease caused by African swine fever virus(ASFV),requires maintenance of high biosecurity standards,which principally relies on disinfection.Finding the perfect disinfectant against ASFV is difficult because of the lack of relevant data.Therefore,we aimed to find the most effective disinfectant and to optimise its concentration as well as contact time to confirm the viricidal effect against ASFV in vitro.We evaluated the viricidal activity of three concentrations each of six common disinfectants against ASFV using immersion disinfection assay(IDA)and spray disinfection assay(SDA);the concentrations of these disinfectants at which complete viral inactivation occurred were almost same as the manufacturer-recommended concentrations,but the exposure times for viral inactivation are different.The following disinfectants(assay:concentration,exposure time)showed complete inactivation:iodine and acid mixed solution(IDA/SDA:0.5%,10 min);compound potassium peroxymonosulfate(IDA:0.25%,30 min;SDA:0.25%,60 min);citric acid(IDA:0.25%,60 min;SDA:0.5%,60 min);sodium dichloroisocyanurate(IDA:0.125%,60 min;SDA:0.25%,60 min);and glutaral ang deciquam(IDA/SDA:0.2%,60 min);and deciquam(IDA/SDA:0.5%,60 min).However,in the presence of organic material contamination,disinfectants did not show a marked inactivation effect.Therefore,disinfection procedures should be performed in two steps:thorough mechanical cleaning followed by application of disinfectant.In conclusion,all the tested disinfectants can inactivate ASFV;these can be used as alternative disinfectants to enhance biosecurity. 展开更多
关键词 DISINFECTION African swine fever virus(ASFV) virucidal effects BIOSECURITY
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