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Involvement of mitogen-activated protein kinase pathways in N-methyl-D-aspartate-induced excitotoxicity 被引量:1
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作者 Xiaorong Yang Ping Sun +5 位作者 Huaping Qin Rui Wang Ye Wang Ruihong Shi Xin Zhao Ce Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第4期288-294,共7页
Previous studies have shown that mitogen-activated protein kinase (MAPK) signaling pathways are involved in N-methyI-D-aspartate (NMDA)-mediated excitotoxicity. However, a systematic observation or analysis of the... Previous studies have shown that mitogen-activated protein kinase (MAPK) signaling pathways are involved in N-methyI-D-aspartate (NMDA)-mediated excitotoxicity. However, a systematic observation or analysis of the role of these various MAPK pathways in excitotoxicity processes does not exist. The present study further evaluated the role and contribution of three MAPK pathways extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 MAPK in an NMDA-mediated excitotoxicity model using MAPK^specific inhibitor. Results demonstrated that c-Jun N-terminal kinase inhibitor SP600125 and/or p38 MAPK inhibitor SB203580 inhibited NMDA-induced reduction in cell viability, as well as reduced NMDA-induced lactate dehydrogenase leakage and reactive oxygen species production. However, PD98059, an inhibitor of extracellular signal-regulated kinase, did not influence this model. Results demonstrated an involvement of c-Jun N-terminal kinase and p38 MAPK, but not extracellular signal-regulated kinase, in NMDA-mediated excitotoxicity in cortical neurons. 展开更多
关键词 cortical neurons EXCITOTOXICITY mitogen-activated protein kinase N-methyI-d-aspartate
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Effect of cyclovirobuxine D on human growth-associated protein 43 and neurocan expression in ischemic brain tissue of stroke-prone renovascular hypertensive rats 被引量:1
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作者 Feng Tan Wei Gu +6 位作者 Saiying Wan Haike Wu Jinliang Wang Jingbo Sun Jiamao Cheng Xin Zhang Renfeng Huang 《Neural Regeneration Research》 SCIE CAS CSCD 2008年第4期394-397,共4页
BACKGROUND: Experimental data indicate that human growth-associated protein 43 mRNA expression coincides with axonal growth during nerve ganglion development; while neurocan, secreted from astrocytes, can inhibit spr... BACKGROUND: Experimental data indicate that human growth-associated protein 43 mRNA expression coincides with axonal growth during nerve ganglion development; while neurocan, secreted from astrocytes, can inhibit sprouting and elongation of the axonal growth cone. OBJECTIVE: To verify regulatory effects of cyclovirobuxine D (CVB-D) extracted from Chinese box branchlet on human growth-associated protein 43 (GAP-43), and neurocan expression in brain tissue of stroke-prone renovascular hypertensive (RHRSP) rats, at different time points after cerebral ischemia/reperfusion. DESIGN: Randomized grouping design and controlled animal study. SETTING: This study was performed at the Center of Guangdong Hospital of Traditional Chinese Medicine (a national key laboratory) from March 2003 to September 2006. MATERIALS: 100 healthy male Sprague-Dawley rats, aged 2 3 months and weighing 90-120 g, were selected for this study. CVB-D was provided by Nanjing Xiaoying Pharmaceutical Factory (Batch number: 307701). METHODS: The initial tip of renal arteries was clamped bilaterally for 10 weeks to establish the RHRSP model. 100 RHRSP rats were randomly divided into 4 groups: naive group (n = 10), sham surgery group (n = 10), CVB-D group (n = 40), and lesion group (n = 40). Rats in the naive group did not undergo any treatment, and cervical vessels of rats in the sham surgery group were exposed, but not blocked. The right middle cerebral artery of rats in the CVB-D group and lesion group were occluded to establish cerebral ischemia. Rats in the CVB-D group were intraperitoneally injected with CVB-D (6.48 mg/kg) every day and with saline (1.5 mL/injection) twice a day. Rats in the lesion group were intraperitoneally injected with saline (2 mL/injection). MAIN OUTCOME MEASURES: Immunohistochemistry was applied to detect GAP-43 and neurocan expression in the ischemic penumbra region of CVB-D and lesion brains at 2 hours post-cerebral ischemia and at 1, 7, 14, and 30 days post-perfusion (n = 10 at each time point). Similarly, GAP-43 and neurocan expression was detected in the right hemisphere of naive and sham-operated animals. The results were expressed as positive cells. RESULTS: A total of 100 rats were included in the final analysis. The number of GAP-43 positive cells increased in the CVB-D group 1, 7, 14, and 30 days post-cerebral ischemia/perfusion compared to the lesion group, as indicated by a significant difference between the CVB-D and lesion group (P 〈 0.054).01). The number of neurocan-positive cells decreased in the CVB-D group on the first day compared to the model group; however, there was no significant difference between the two groups (P 〉 0.05). On post-ischemia days 7, 14, and 30, the number of neurocan-positive cells in the CVB-D group was significantly less than in the lesion group (P 〈 0.05). Both, GAP-43 and neurocan expression was not detectable in brains of naive and sham-operated animals. CONCLUSION: CVB-D treatment up-regulated GAP-43 expression and down-regulated neurocan expression in the ischemic region of RHRSP rats. 展开更多
关键词 cerebral ischemia/perfusion human growth-associated protein 43 NEUROCAN cyclovirobuxine d rats
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N-methyl-D-aspartate receptor effects on p38 mitogen activated protein kinase and its role in a rat model of diabetic neuropathic pain
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作者 Changbin Ke Xiaoxia Huang +4 位作者 Xianghong Luo Xiancheng Xu Chengming Qin Xianyu Wang Juying Liu 《Neural Regeneration Research》 SCIE CAS CSCD 2009年第11期868-873,共6页
BACKGROUND: Activated N-methyl-D-aspartate (NMDA) receptor is involved in the formation of chronic neuropathic pain, and its antagonist, ketamine, exhibits effective amelioration of diabetic neuropathic pain (DNP... BACKGROUND: Activated N-methyl-D-aspartate (NMDA) receptor is involved in the formation of chronic neuropathic pain, and its antagonist, ketamine, exhibits effective amelioration of diabetic neuropathic pain (DNP). However, the mechanisms of NMDA receptor participation in the formation and maintenance of DNP remain poorly understood. OBJECTIVE: To evaluate the role NMDA receptor plays in DNP and effects on p38 mitogen activated protein kinase (p38 MAPK) in a rat model of DNP. DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed at the Human Embryonic Stem Cell Research Institute of Yunyang Medical College Affiliated Taihe Hospital between July 2005 and September 2007. MATERIALS: Streptozotocin was provided by Sigma, USA; p38 MAPK inhibitor (SB203580) was provided by Shanghai KangChen Biotech, China; NMDA receptor antagonist (MK-801) was purchased from Shanghai Yope Biotech, China. METHODS: A total of 128 healthy, Wistar rats of clean grade, aged 3 months and weighing 180- 220 g, were randomly assigned to 4 groups: control, DNP model, p38 MAPK, and NMDA receptor. Each group contained 32 rats. DNP was established in all groups except for the control group by intraperitoneal injection of streptozocin (65 mg/kg). Subsequently, 1 mg/kg SB203580 and 1 mg/kg MK-801 were injected once each week via intraperitoneal injection in the p38 MAPK and NMDA receptor groups, respectively. MAIN OUTCOME MEASURES: At the end of 2, 4, 6, and 8 weeks following streptozotocin injection, mechanical withdrawal threshold was measured in 8 animals from each group following von Frey filament stimulation. The rats were anesthetized and nerve conduction velocity of the left sciatic nerve was measured. Subsequently, the right sciatic nerve, the lumbar segment of the spinal cord, and dorsal root ganglia were removed from the L3-6 segment for microscopic examination, p38 MAPK expression was determined using immunohistochemistry and Western blot analysis. Expression of NMDA receptor 1 mRNA in dorsal root ganglion and spinal cord neurons was detected using RT-PCR. RESULTS: Mechanical withdrawal threshold and nerve conduction velocity were significantly reduced, and p38 MAPK and NMDA receptor 1 mRNA expression in the spinal cord and dorsal root ganglia were significantly increased, in the model, p38 MAPK, and NMDA receptor groups compared with the control group at all time points (P 〈 0.05). At 4-8 weeks following successful DNP model establishment, SB203580 and MK-801 increased mechanical withdrawal threshold, accelerated nerve conduction velocity, and attenuated p38 MAPK expression, compared with the model group. The NMDA receptor group exhibited downregulated mRNA expression of NMDA receptor 1 compared with the model and p38 MAPK groups (P 〈 0.05). CONCLUSION: NMDA receptor was highly expressed in the brains of DNP rats and was involved in DNP development via activation of the p38 MAPK signal pathway. 展开更多
关键词 N-methyI-d-aspartate receptors p38 mitogen-activated protein kinases diabetic neuropathy NEURALGIA
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Mechanisms mediating the effects of alcohol and HIV anti-retroviral agents on mTORC1,mTORC2 and protein synthesis in myocytes 被引量:2
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作者 Ly Q Hong-Brown Abid A Kazi Charles H Lang 《World Journal of Biological Chemistry》 CAS 2012年第6期110-120,共11页
Alcoholism and acquired immune deficiency syndrome are associated with severe muscle wasting.This impairment in nitrogen balance arises from increased protein degradation and a decreased rate of protein synthesis.The ... Alcoholism and acquired immune deficiency syndrome are associated with severe muscle wasting.This impairment in nitrogen balance arises from increased protein degradation and a decreased rate of protein synthesis.The regulation of protein synthesis is a complex process involving alterations in the phosphorylation state and protein-protein interaction of various components of the translation machinery and mammalian target of rapamycin(mTOR) complexes.This review describes mechanisms that regulate protein synthesis in cultured C2C12 myocytes following exposure to either alcohol or human immunodeficiency virus antiretroviral drugs.Particular attention is given to the upstream regulators of mTOR complexes and the downstream targets which play an important role in translation.Gaining a better understanding of these molecular mechanisms could have important implications for preventing changes in lean body mass in patients with catabolic conditions or illnesses. 展开更多
关键词 AMP-activated protein kinase/tuberous sclerosis complex 2/Ras homolog enriched in brain Rag GTPASES PHOSPHOLIPASE d MITOGEN-ACTIVATEd protein KINASE Translation initiation Elongation
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Evaluation of the Dietary Reference Intakes for Japanese (2010 Edition): Recommended Protein, Pantothenic acid, Vitamin D, and Iron Intakes for Breast-Fed Infants Aged 6 - 11 Months 被引量:1
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作者 Setsuko Tsutie Nobutaka Kurihara +3 位作者 Aki Sasaki Arisa Takagi Harumiti Seguti Tetsuya Inatome 《Food and Nutrition Sciences》 2011年第4期272-280,共9页
Objective: With regard to the 2010 edition of Dietary Reference Intakes for Japanese (DRIs-2010), we investigated whether the DRIs for two age groups, breast-fed infants aged 6-8 and 9-11 months, can be fulfilled for ... Objective: With regard to the 2010 edition of Dietary Reference Intakes for Japanese (DRIs-2010), we investigated whether the DRIs for two age groups, breast-fed infants aged 6-8 and 9-11 months, can be fulfilled for every nutrient in actual dietary practice. Design: We evaluated (1) whether the DRIs for all nutrients can be fulfilled in a formula with energy and protein exceeding their DRIs, (2) whether the DRIs for all nutrients can be fulfilled in a formula prepared in accordance with Japanese government-recommended weaning guidelines, and (3) what kinds of formulas can be prepared if the DRIs for all nutrients are fulfilled without referring to the weaning guidelines. Setting: Simulation of diet menu on the basis of published data in our university and survey of diet menu in a university hospital attached to a national medical school. Subjects: The three types of formulas were planned for ten days. Results: It was impossible to simultaneously fulfil the DRIs for 6 - 8-month-old infants concerning pantothenic acid, vitamin D, and iron and those for 9 - 11-month-old infants concerning these nutrients plus protein. Conclusion: According to the DRIs-2010, the DRI for all nutrients could not be fulfilled in an ingestible formula. 展开更多
关键词 dietary Reference INTAKES Breast-Fed INFANTS (6 - 11-Month-Old) Pantothenic Acid protein Vitamin d Iron
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Comparative analysis of bed density, total phenol content and protein expression pattern in <i>Posidonia oceanica</i>(L.) Delile
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作者 Alice Rotini Letizia Anello +3 位作者 Maria Di Bernardo Agata Giallongo Luigi Valiante Luciana Migliore 《Open Journal of Ecology》 2013年第6期438-444,共7页
Posidonia oceanicameadows are experiencing a progressive decline, and monitoring their status is crucial for the maintenance of theseecosystems. We performed a comparativeanalysis of bed density, total phenol content ... Posidonia oceanicameadows are experiencing a progressive decline, and monitoring their status is crucial for the maintenance of theseecosystems. We performed a comparativeanalysis of bed density, total phenol content and protein expression pattern to assess the conservation status ofPosidoniaplants from the S. Marinella (Rome, Italy) meadow. The total phenol content was inversely related to maximum beddensity, confirming the relationship betweenhigh phenol content and stressful conditions. In addition, protein expression pattern profilesshowed that the number of differentially expressed proteins was dramatically reduced in the latest years compared to previous analyses. Our results support the usefulness of integrating solid descriptors, such as phenol content, with novel biochemical/molecular approaches in the monitoring of meadows. 展开更多
关键词 POSIdONIA oceanica Rhizome Seagrass Monitoring Total Phenols 2-d Electrophoresis (2-dE) BEd dENSITY protein Expression Pattern
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Effect of high salinity on cell growth and protein production of Antarctic ice microalgae Chlamydomonas sp. ICE-L
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作者 郑洲 缪锦来 +5 位作者 阚光峰 金青 丁燏 刘芳明 王守强 王以斌 《Chinese Journal of Polar Science》 2010年第1期81-90,共10页
Antarctic ice microalgae Chlamydomonas sp.ICE-L can survive and thrive in Antarctic sea ice.In this study,Chlamydomonas sp.ICE-L could survive at the salinity of 132‰ NaCl.SDS-PAGE showed that the density of 2 bands... Antarctic ice microalgae Chlamydomonas sp.ICE-L can survive and thrive in Antarctic sea ice.In this study,Chlamydomonas sp.ICE-L could survive at the salinity of 132‰ NaCl.SDS-PAGE showed that the density of 2 bands(26 and 36 kD) decreased obviously at the salinity of 99‰ NaCl compared to at the salinity of 33‰ NaCl.The soluble proteins in Chlamydomonas sp.ICE-L grown under salinity of 33‰ and 99% NaCl were compared by 2-D gel electrophoresis.After shocking with high salinity,8 protein spots were found to disappear,and the density of 28 protein spots decreased.In addition,19 protein spots were enhanced or induced,including one new peptide(51 kD).The changes of proteins might be correlated with the resistance for Chlamydomonas sp.ICE-L to high salinity. 展开更多
关键词 Antarctic ice alga Chlamydomonas sp.ICE-L soluble proteins two-dimensional polyacrylamide gel electrophoresis gel electrophoresis(2-d PAGE) analysis high-salinity press.
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血清D-D、GSP、HbA1c水平对妊娠糖尿病的临床意义 被引量:1
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作者 高萃 崔金全 邵文嘉 《河南医学研究》 CAS 2024年第11期1997-2001,共5页
目的 探究检测血清D-二聚体(D-D)、糖化血清蛋白(GSP)、糖化血红蛋白(HbA1c)表达水平在妊娠糖尿病中的临床意义。方法 选取2020年1月至2022年12月于郑州大学第二附属医院就诊117例妊娠糖尿病患者为研究组,同期选择117例体检健康孕妇作... 目的 探究检测血清D-二聚体(D-D)、糖化血清蛋白(GSP)、糖化血红蛋白(HbA1c)表达水平在妊娠糖尿病中的临床意义。方法 选取2020年1月至2022年12月于郑州大学第二附属医院就诊117例妊娠糖尿病患者为研究组,同期选择117例体检健康孕妇作为对照组进行研究,比较两组血清D-D、GSP、HbA1c水平及稳态模型胰岛素抵抗指数(HOMA-IR),采用Pearson分析入院时血清D-D、GSP、HbA1c水平与HOMA-IR相关性,受试者工作特征(ROC)曲线分析血清D-D、GSP联合HbA1c水平检测对妊娠糖尿病病情程度的评估价值。结果 与对照组相比,研究组血清D-D、GSP、HbA1c水平及HOMA-IR均升高(P<0.05)。不同病情程度孕妇的血清D-D、GSP、HbA1c水平及HOMA-IR指数比较:重度>中度>轻度(P<0.05)。血清D-D、GSP、HbA1c水平与HOMA-IR均呈正相关(r=0.671、0.715、0.696,P<0.05)。产检时血清D-D、GSP、HbA1c水平联合诊断中度、重度妊娠糖尿病的曲线下面积(AUC)分别为0.859、0.873,最佳敏感度分别为95.64%、96.30%,特异度分别为76.12%、78.26%。结论 血清D-D、GSP、HbA1c水平联合检测可为临床评估妊娠糖尿病病情程度提供可靠参考依据。 展开更多
关键词 d-二聚体 糖化血清蛋白 糖化血红蛋白 妊娠糖尿病
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D-二聚体与C反应蛋白对Stanford A型主动脉夹层动脉瘤患者术后远期预后的预测价值 被引量:1
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作者 屈冰 张楠 +1 位作者 化召辉 李震 《实用癌症杂志》 2024年第2期327-330,共4页
目的分析D-二聚体(D-D)与C反应蛋白(CRP)在Stanford A型主动脉夹层动脉瘤(ADA)患者术后远期预后中的预测价值。方法选取76例胸痛患者为研究对象,所有患者均行心脏超声或主动脉CT血管成像检查,将45例Stanford A型ADA患者纳入观察组,均行... 目的分析D-二聚体(D-D)与C反应蛋白(CRP)在Stanford A型主动脉夹层动脉瘤(ADA)患者术后远期预后中的预测价值。方法选取76例胸痛患者为研究对象,所有患者均行心脏超声或主动脉CT血管成像检查,将45例Stanford A型ADA患者纳入观察组,均行手术治疗;非Stanford A型ADA的31例患者纳入对照组。对比2组发生胸痛72 h内的D-D与CRP水平。术后对观察组随访3年,按临床结局分成死亡组(n=10)与存活组(n=35),对比2组D-D与CRP水平;另绘制受试者工作曲线(ROC),分析D-D、CRP单独与联合预测Stanford A型ADA患者的远期预后临床价值。结果观察组的D-D[(1.31±0.35)mg/L]与CRP[(16.59±2.34)mg/L]水平高于对照组[(0.68±0.13)mg/L、(7.53±1.26)mg/L],差异有统计学意义(P<0.05);ADA患者中死亡组的D-D[(1.78±0.49)mg/L]与CRP[(30.46±4.77)mg/L]水平高于存活组[(1.03±0.26)mg/L、(13.59±2.36)mg/L],差异有统计学意义(P<0.05)。ROC结果显示,D-D与CRP联合检测预测Stanford A型ADA患者远期预后的曲线下面积(AUC)[0.909(95%CI:0.823~0.994)]高于D-D与CRP单独预测[0.806(95%CI:0.680~0.931)、0.840(95%CI:0.727~0.953)]。结论Stanford A型ADA患者血清内的D-D与CRP水平呈高表达,且表达水平越高,患者预后越差,两项指标联合可有效预测患者的远期预后,临床应用价值较高。 展开更多
关键词 主动脉夹层动脉瘤 d-二聚体 C反应蛋白 远期预后 预测价值
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肠脂肪酸结合蛋白和D-乳酸在早期诊断嵌顿疝肠坏死中的应用研究
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作者 满艺 石松荔 +2 位作者 李宝山 黄皇 王荫龙 《中国中西医结合外科杂志》 CAS 2024年第2期274-278,共5页
目的:探讨肠脂肪酸结合蛋白(I-FABP)和D-乳酸(D-LAC)早期诊断嵌顿疝肠坏死的价值。方法:选取36只SD大鼠,实验组(n=18)制作嵌顿疝动物模型,对照组(n=18)未制作。在术后30 min、2 h、4 h、6 h、8 h和12 h,采用ELISA检测两组血清D-LAC和I-F... 目的:探讨肠脂肪酸结合蛋白(I-FABP)和D-乳酸(D-LAC)早期诊断嵌顿疝肠坏死的价值。方法:选取36只SD大鼠,实验组(n=18)制作嵌顿疝动物模型,对照组(n=18)未制作。在术后30 min、2 h、4 h、6 h、8 h和12 h,采用ELISA检测两组血清D-LAC和I-FABP的水平;RT-qPCR鉴定嵌顿疝肠管组织中I-FABP的表达。通过嵌顿肠管大体标本、苏木素伊红(HE)染色和Chiu’s评分判定肠坏死情况。结果:与对照组相比,实验组在术后6 h时嵌顿肠管大体标本和HE染色呈典型肠绞窄表现,Chiu’s评分有统计学意义(P=0.001),血D-LAC明显升高(P=0.002);8 h时肠管逐渐向肠坏死过渡,血D-LAC进一步升高(P=0.012),血I-FABP也明显升高(P=0.001),并且肠组织中的I-FABP表达明显升高(P=0.002)。12 h时肠管呈现明显肠坏死特征、Chiu’s评分有统计学意义(P=0.001),血D-LAC和I-FABP均升至最高[(2019.60±16.17)μg/L vs(273.18±14.63)μg/L,P=0.001;(1210.94±5.96)μg/L vs(220.46±9.63)μg/L,P=0.001];肠管组织中的I-FABP表达最高[(8.20±0.60)μg/L vs(1.13±0.16)μg/L,P=0.001]。结论:嵌顿疝大鼠血清I-FABP和D-LAC水平升高,为早期诊断嵌顿疝肠管坏死的临床研究提供了依据。 展开更多
关键词 嵌顿疝 肠坏死 肠脂肪酸结合蛋白 d-乳酸
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血清维生素D结合蛋白、FGF23、Klotho与乳腺癌骨转移的相关性分析
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作者 王一 廖宏伟 《循证医学》 2024年第1期44-50,共7页
目的骨转移是乳腺癌常见的并发症之一,严重影响患者的生存和预后。本研究旨在探究血清维生素D结合蛋白(vitamin D⁃binding protein,VDBP)、成纤维细胞生长因子23(fibroblast growth factor 23,FGF23)和Klotho蛋白在乳腺癌骨转移中的表... 目的骨转移是乳腺癌常见的并发症之一,严重影响患者的生存和预后。本研究旨在探究血清维生素D结合蛋白(vitamin D⁃binding protein,VDBP)、成纤维细胞生长因子23(fibroblast growth factor 23,FGF23)和Klotho蛋白在乳腺癌骨转移中的表达及临床意义。方法收集95例来自本院2019⁃08⁃01至2021⁃08⁃01的女性乳腺癌患者作为研究对象,经影像学和组织病理学方式诊断是否发生骨转移,将患者分为骨转移组36例,非骨转移组59例。分析两组患者的临床病理特征;采集患者外周血样本,通过ELISA对血清中VDBP、FGF23和Klotho进行定量分析;使用Spearman相关分析进行指标间的关联性分析;Logistic回归分析乳腺癌发生骨转移的影响因素;ROC曲线分析血清VDBP、FGF23和Klotho水平预测乳腺癌发生骨转移的价值。结果骨转移和非骨转移乳腺癌病理分级比较有统计学意义(P<0.05)。骨转移和非骨转移乳腺癌患者血清中VDBP、FGF23及Klotho的水平依次为:(80.35±29.34)和(115.18±48.69)ng/mL、(658.35±201.19)和(405.36±154.42)pg/mL以及(155.82±40.29)和(229.35±72.46)pg/mL,两组比较差异有统计学意义(P<0.05)。Spearman相关性分析显示,骨转移乳腺癌患者血清中VDBP水平与乳腺癌病理分级相关(P<0.05);FGF23和Klotho水平与病理分级、是否骨痛以及转移部位有关(P<0.05)。VDBP、FGF23和Klotho水平均为乳腺癌骨转移发生的独立影响因素(P<0.05)。ROC曲线结果显示,VDBP、FGF23及Klotho预测乳腺癌患者发生骨转移的曲线下面积依次为:0.733、0.806、0.761,最佳截断值为:81.56 ng/mL、573.501 pg/mL和201.193 pg/mL;3个指标联合诊断的曲线下面积为0.820,高于单一指标诊断的曲线下面积。结论血清VDBP、FGF23及Klotho水平可作为乳腺癌骨转移的参考指标,在乳腺癌骨转移的临床诊断上具有一定应用前景。 展开更多
关键词 乳腺癌 骨转移 维生素d结合蛋白 成纤维细胞生长因子23 KLOTHO
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感染性肺炎新生儿的血清25-羟基维生素D与炎症因子的相关性分析
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作者 曹沐琳 杜志云 +3 位作者 邱锐琴 乔木 韩雁雁 姚文秀 《海军医学杂志》 2024年第2期186-189,共4页
目的分析感染性肺炎新生儿的血清25-羟基维生素D[25-hydroxyvitamin D,25(OH)D]与炎症因子干扰素-γ(infectious pneumonia-γ,IFN-γ)、C-反应蛋白(C-reactive protein,CRP)、白细胞介素-2(interleukin-2,IL-2)水平的相关性。方法选取... 目的分析感染性肺炎新生儿的血清25-羟基维生素D[25-hydroxyvitamin D,25(OH)D]与炎症因子干扰素-γ(infectious pneumonia-γ,IFN-γ)、C-反应蛋白(C-reactive protein,CRP)、白细胞介素-2(interleukin-2,IL-2)水平的相关性。方法选取河北省秦皇岛市第一医院2018年12月至2020年12月收治的100例感染性肺炎新生儿,根据血清25(OH)D水平分为缺乏组(≤15.0μg/L,18例)、不足组(15.1~20.0μg/L,42例)、充足组(>20.0μg/L,40例)。统计3组性别、胎龄、血清25(OH)D水平、出生体重、分娩方式等一般资料和临床资料,比较3组IFN-γ、CRP及IL-2水平,分析新生儿血清25(OH)D水平与IFN-γ、CRP、IL-2水平的相关性。结果3组胎龄、性别、出生体质量、分娩方式比较差异无统计学意义(P>0.05);缺乏组、不足组、充足组新生儿的血清25(OH)D水平逐渐升高,差异有统计学意义(P<0.05)。缺乏组IFN-γ、CRP及IL-2水平均高于不足组、充足组(P<0.05),不足组IFN-γ、CRP及IL-2水平高于充足组(P<0.05)。经Pearson相关分析,感染性肺炎新生儿血清25(OH)D水平与IFN-γ、CRP、IL-2水平呈负相关(P<0.05)。结论新生儿血清25(OH)D越低,维生素D越缺乏,IFN-γ、CRP及IL-2水平越高,感染性肺炎的风险越高。 展开更多
关键词 新生儿 维生素d 干扰素-Γ C-反应蛋白 白细胞介素-2
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Standford B型主动脉夹层腔内治疗围手术期超敏C反应蛋白等相关指标应用意义的临床研究
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作者 吴跃武 胡斌 +1 位作者 付琴 过小冬 《中国当代医药》 CAS 2024年第10期62-65,70,共5页
目的探讨超敏C反应蛋白(hs-CRP)、D-二聚体(D-D)、降钙素原(PCT)对Standford B型主动脉夹层(TBAD)患者胸主动脉腔内修复术预后的影响。方法选取2017年4月至2022年3月抚州市第一人民医院行胸主动脉腔内修复术(TEVAR)的80例TBAD患者作为... 目的探讨超敏C反应蛋白(hs-CRP)、D-二聚体(D-D)、降钙素原(PCT)对Standford B型主动脉夹层(TBAD)患者胸主动脉腔内修复术预后的影响。方法选取2017年4月至2022年3月抚州市第一人民医院行胸主动脉腔内修复术(TEVAR)的80例TBAD患者作为研究对象,于术前1 d和术后7 d检测hs-CRP、D-D、PCT水平。根据患者预后分为预后良好组和预后不良组,比较两组患者术前1 d和术后7 d的血清hs-CRP、D-D、PCT水平,分析血清hs-CRP、D-D、PCT水平与患者预后的关联性。结果80例患者中预后良好(预后良好组)63例(78.75%),预后不良(预后不良组)17例(21.25%)。两组患者术后7 d血清hs-CRP、D-D、PCT水平高于本组术前1 d,差异有统计学意义(P<0.05);预后良好组患者术前1 d和术后7 d的血清hs-CRP、D-D、PCT水平低于预后不良组,差异有统计学意义(P<0.05);logistic回归模型分析结果显示,术前1 d血清hs-CRP(β=0.617,OR=1.854,95%CI=1.217~2.696)、D-D(β=0.639,OR=1.895,95%CI=1.841~2.635)、PCT(β=0.554,OR=1.741,95%CI=1.547~3.052)和术后7 d hs-CRP(β=0.892,OR=2.440,95%CI=1.251~4.761)、D-D(β=0.797,OR=2.219,95%CI=1.669~3.141)、PCT(β=0.604,OR=1.829,95%CI=1.058~2.969)水平是接受TEVAR治疗的TBAD患者预后的影响因素,差异有统计学意义(P<0.05)。结论监测围手术期血清hs-CRP、D-D、PCT水平对接受TEVAR治疗的TBAD患者预后判断具有一定的参考价值。 展开更多
关键词 Standford B型 主动脉夹层 腔内治疗 围手术期 超敏C反应蛋白 d-二聚体 降钙素原
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NT-proBNP、D-D、CRP检测在中老年急性脑梗死患者中的临床诊断价值分析 被引量:1
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作者 张伟 《中国实用医药》 2024年第10期81-84,共4页
目的 分析氨基末端脑钠肽前体(NT-proBNP)、D-二聚体(D-D)、C反应蛋白(CRP)检测对中老年急性脑梗死(ACI)患者的诊断价值。方法 选取中老年急性脑梗死患者52例为观察组[根据病灶直径不同分为大面积梗死组(>5 cm)10例、中面积梗死组(≤... 目的 分析氨基末端脑钠肽前体(NT-proBNP)、D-二聚体(D-D)、C反应蛋白(CRP)检测对中老年急性脑梗死(ACI)患者的诊断价值。方法 选取中老年急性脑梗死患者52例为观察组[根据病灶直径不同分为大面积梗死组(>5 cm)10例、中面积梗死组(≤5 cm,>3 cm)16例、小面积梗死组(≤3 cm,>1.5 cm)26例],另选取健康体检者50例为对照组。观察组及对照组均进行D-D、CRP、NT-proBNP水平检测。比较观察组及对照组的CRP、D-D、NT-proBNP水平,观察组不同脑梗死面积患者的CRP、D-D、NT-proBNP水平。结果观察组CRP、D-D、NT-proBNP水平均高于对照组,差异具有统计学意义(P<0.05)。观察组中,大面积梗死组的NT-proBNP、D-D、CRP水平分别为(3351.70±940.97)pg/ml、(898.43±323.19)ng/ml、(41.79±11.08)mg/L,中面积梗死组的NT-proBNP、D-D、CRP水平分别为(1338.06±345.23)pg/ml、(573.88±312.67)ng/ml、(32.05±11.77)mg/L,小面积梗死组的NT-proBNP、D-D、CRP水平分别为(447.00±195.72)pg/ml、(334.15±229.81)ng/ml、(18.03±10.14)mg/L。大面积梗死组患者的NT-proBNP、D-D、CRP水平均高于中面积梗死组及小面积梗死组,中面积梗死组患者的NT-proBNP、D-D、CRP水平均高于小面积梗死组,差异具有统计学意义(P<0.05)。结论 D-D、CRP、NT-proBNP在诊断中老年急性脑梗死患者中存在较高的应用价值。 展开更多
关键词 d-二聚体 C反应蛋白 氨基末端脑钠肽前体 急性脑梗死 诊断价值
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2型糖尿病患者维生素D水平与血清CRP、自身免疫抗体及甲状腺功能的相关性
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作者 姚莉敏 付士玲 郎少磊 《河南医学研究》 CAS 2024年第16期2988-2992,共5页
目的研究2型糖尿病患者维生素D水平与血清C反应蛋白(CRP)、自身免疫抗体及甲状腺功能的相关性。方法选取2018年1月至2019年1月于信阳职业技术学院附属医院就诊的150例2型糖尿病患者为研究组,同期选取50例健康体检者作为对照组。检测血清... 目的研究2型糖尿病患者维生素D水平与血清C反应蛋白(CRP)、自身免疫抗体及甲状腺功能的相关性。方法选取2018年1月至2019年1月于信阳职业技术学院附属医院就诊的150例2型糖尿病患者为研究组,同期选取50例健康体检者作为对照组。检测血清CRP、血清25-羟维生素D[25(OH)D]、胰岛自身免疫抗体和甲状腺功能指标水平并比较两组差异;按血清25(OH)D水平将研究组分为正常组(34例)、不足组(37例)、缺乏组(39例)和严重缺乏组(40例)4个亚组,比较组间血清CRP、胰岛自身免疫抗体阳性率和甲状腺功能指标水平差异;对血清25(OH)D与CRP、胰岛自身免疫抗体阳性率及甲状腺功能指标水平的相关性进行Pearson和Spearman相关性分析;随访3 a,统计甲状腺功能障碍发生率,并绘制受试者工作特征(ROC)曲线分析2型糖尿病患者血清25(OH)D对甲状腺功能障碍的预测效能。结果与对照组比较,研究组血清25(OH)D、TSH水平较低,CRP水平和胰岛自身免疫抗体阳性率较高(P<0.05)。正常组、不足组、缺乏组和严重缺乏组的血清CRP、胰岛自身免疫抗体阳性率和TSH水平比较差异有统计学意义,其中血清CRP、TSH水平逐渐上升(P<0.05)。2型糖尿病患者血清25(OH)D水平与CRP(r=-0.714)、胰岛自身免疫抗体阳性率(r=-0.397)和TSH(r=-0.410)水平均呈负相关(P<0.001)。2型糖尿病患者甲状腺功能障碍发生率为25.33%(38/150),绘制ROC曲线分析血清25(OH)D预测甲状腺功能障碍发生的AUC为0.916。结论2型糖尿病患者血清维生素D与CRP、自身免疫抗体阳性率以及甲状腺功能指标水平存在负相关关系,且对甲状腺功能障碍的发生有一定预测价值。 展开更多
关键词 2型糖尿病 C反应蛋白 维生素d 自身免疫抗体 甲状腺功能
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血清CXCL9、sCD163、SP-D含量在老年慢性阻塞性肺疾病急性加重期近期预后中的预测价值
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作者 邓宝娟 李生香 +1 位作者 刘娜娜 高秀 《临床和实验医学杂志》 2024年第19期2026-2029,共4页
目的分析血清CXC趋化因子配体9(CXCL9)、可溶性血红蛋白清道夫受体163(sCD163)、肺表面活性蛋白D(SP-D)含量在老年慢性阻塞性肺疾病急性加重期(AECOPD)近期预后中的预测价值。方法回顾性分析2021年4月至2023年4月在榆林市第一医院进行... 目的分析血清CXC趋化因子配体9(CXCL9)、可溶性血红蛋白清道夫受体163(sCD163)、肺表面活性蛋白D(SP-D)含量在老年慢性阻塞性肺疾病急性加重期(AECOPD)近期预后中的预测价值。方法回顾性分析2021年4月至2023年4月在榆林市第一医院进行治疗的110例老年AECOPD患者的临床资料。依据随访3个月的预后情况,分为预后良好组(n=75)、预后不良组(n=35)。比较两组基线资料,通过多因素Logistic回归分析老年AECOPD近期预后不良的危险因素,采取非条件Logistic逐步回归分析受试者操作特征(ROC)曲线预测急性生理与慢性健康评分Ⅱ(APACHEⅡ)、血清CXCL9、sCD163、SP-D老年AECOPD近期预后的价值。结果预后不良组APACHEⅡ评分、血清CXCL9、sCD163、SP-D水平分别为(25.47±6.52)分、(12.16±3.21)pmol/L、(145.56±30.12)pg/mL、(137.21±35.47)μg/L,均高于预后良好组[(17.42±5.84)分、(9.45±3.12)pmol/L、(104.54±25.37)pg/mL、(113.45±30.67)μg/L],差异均有统计学意义(P<0.05)。多因素Logistic回归分析证实,APACHEⅡ评分、血清CXCL9、sCD163、SP-D是老年AECOPD近期预后不良的危险因素,均有P<0.05;经ROC曲线分析证实APACHEⅡ评分、血清CXCL9、sCD163、SP-D均可用于老年AECOPD近期预后的预测,曲线下面积分别为0.723、0.821、0.815、0.840,均有较高预测价值(P<0.05)。结论血清CXCL9、sCD163、SP-D水平升高将增加老年AECOPD患者近期预后不良风险,可将以上指标用于预测老年AECOPD患者近期预后,可获得较高敏感度与特异度。 展开更多
关键词 老年人 CXC趋化因子配体9 可溶性血红蛋白清道夫受体163 肺表面活性蛋白d 慢性阻塞性肺疾病急性加重期 预后
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首次复治菌阳肺结核合并糖尿病患者血清IAP、25(OH)D水平及对疗效的预测价值
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作者 王玉红 刘丽景 +3 位作者 杨帆 王智慧 董雅坤 李晓倩 《标记免疫分析与临床》 CAS 2024年第4期642-646,726,共6页
目的探讨首次复治菌阳肺结核(TB)合并糖尿病(DM)患者血清免疫抑制酸性蛋白(IAP)、25-羟基维生素D[25(OH)D]表达变化及2者对治疗疗效的预测价值。方法选取本院2019年1月至2022年4月首次复治菌阳TB合并DM患者48例为实验组,另选同期单纯TB... 目的探讨首次复治菌阳肺结核(TB)合并糖尿病(DM)患者血清免疫抑制酸性蛋白(IAP)、25-羟基维生素D[25(OH)D]表达变化及2者对治疗疗效的预测价值。方法选取本院2019年1月至2022年4月首次复治菌阳TB合并DM患者48例为实验组,另选同期单纯TB患者48例为对照组。测定并比较两组患者治疗前后血清IAP、25(OH)D水平;多因素Logistic回归分析影响菌阳TB合并DM患者痰菌阴转的可能因素;受试者工作特征(ROC)曲线评估IAP、25(OH)D对患者痰菌未转阴的预测价值。结果实验组痰菌阴转患者31例(64.58%)显著低于对照组痰菌阴转患者40例(83.33%)。治疗2个月后,实验组、对照组血清IAP均显著降低,25(OH)D水平均显著升高(P<0.05);且治疗2个月后实验组血清IAP水平显著低于对照组,25(OH)D水平显著高于对照组(P<0.05)。多因素Logistic回归分析显示,IAP高水平是首次复治菌阳TB合并DM患者痰菌未阴转的危险因素(P<0.05),25(OH)D高水平是首次复治菌阳TB合并DM患者痰菌未阴转的保护因素(P<0.05)。ROC曲线显示,IAP预测痰菌未阴转的AUC为0.719,25(OH)D预测痰菌未阴转的AUC为0.791,2者联合预测痰菌未阴转的AUC为0.871,明显高于2者单独诊断(Z_(联合vs IAP)=2.605、P=0.009;Z_(联合vs 25(OH)D)=2.408、P=0.016),且灵敏度、特异性分别为78.46%、82.86%。结论首次复治菌阳TB合并DM患者经治疗后血清IAP水平降低、25(OH)D水平升高,2者联合对菌阳TB合并DM患者痰菌未阴转具有一定预测价值。 展开更多
关键词 复治菌阳肺结核 糖尿病 免疫抑制酸性蛋白 25-羟基维生素d 疗效 预测价值
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骨质疏松症患者PINP、25-(OH)VitD_(3)、BMP-2与中医辨证分型的相关性研究
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作者 刘莎莎 郭洪科 《四川中医》 2024年第6期87-91,共5页
目的:分析骨质疏松症患者血清总I型胶原氨基末端前肽(PINP)、25-羟维生素D3[25-(OH)VitD_(3)]及骨形态发生蛋白2(BMP-2)与其中医辨证分型的相关性。方法:收集我院2020年8月~2023年8月医院收治的157例骨质疏松症患者的一般资料及中医四... 目的:分析骨质疏松症患者血清总I型胶原氨基末端前肽(PINP)、25-羟维生素D3[25-(OH)VitD_(3)]及骨形态发生蛋白2(BMP-2)与其中医辨证分型的相关性。方法:收集我院2020年8月~2023年8月医院收治的157例骨质疏松症患者的一般资料及中医四诊资料进行回顾性分析,并统计骨质疏松症患者中医辨证分型结果,对不同症型患者基本资料、PINP、25-(OH)VitD_(3)、BMP-2水平进行比较。结果:157例骨质疏松症患者中医辨证分型属肾阳虚证34例,脾肾阳虚证43例,肝肾阴虚证49例,血瘀气滞证31例。不同中医辨证分型的骨质疏松患者血清PINP、25-(OH)VitD_(3)及BMP-2水平整体相比,差异有统计学意义(P<0.05);其中,血瘀气滞组PINP水平显著高于肾阳虚组、脾肾阳虚组及肝肾阴虚组患者(P<0.05),而其余3组两两间相比,差异无统计学意义(P>0.05);脾肾阳虚证患者25-(OH)VitD_(3)水平明显低于肾阳虚组、肝肾阴虚组及血瘀气滞组(P<0.05),且肾阳虚组低于肝肾阴虚组及血瘀气滞组(P<0.05),而其余两组相比差异无统计学意义(P>0.05);血瘀气滞组BMP-2水平显著低于肾阳虚组、脾肾阳虚组及肝肾阴虚组患者(P<0.05),而其余3组两两间相比,差异无统计学意义(P>0.05);二分类logistics回归分析结果显示,血瘀气滞与PINP呈正相关(P<0.05),与BMP-2呈负相关(P<0.05);脾肾阳虚与25-(OH)VitD_(3)呈负相关(P<0.05)。结论:骨质疏松症患者的血清PINP、25-(OH)VitD_(3)、BMP-2水平与其中医辨证分型具有一定相关性,可作为评估患者中医辨证分型的参考指标。 展开更多
关键词 骨质疏松症 总I型胶原氨基末端前肽 25-羟维生素d3 骨形态发生蛋白2 中医辨证分型
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先天性巨结肠患儿术后肠道组织中CAD、SOX2的表达水平及其临床意义
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作者 张宁 岳铭 袁宇航 《海南医学》 CAS 2024年第7期939-943,共5页
目的探讨先天性巨结肠(HD)患儿术后肠道组织蛋白酶D(CAD)、性别决定区Y框蛋白2(SOX2)的表达水平及其临床意义。方法选取2015年5月至2019年3月郑州大学第一附属医院收治的56例HD患儿结肠组织(HD组)和23例因肠道梗阻或穿孔实施造瘘手术获... 目的探讨先天性巨结肠(HD)患儿术后肠道组织蛋白酶D(CAD)、性别决定区Y框蛋白2(SOX2)的表达水平及其临床意义。方法选取2015年5月至2019年3月郑州大学第一附属医院收治的56例HD患儿结肠组织(HD组)和23例因肠道梗阻或穿孔实施造瘘手术获取的结肠组织标本(对照组)。采用免疫组化、Western-blot技术检测结肠组织中CAD、SOX2的表达水平,并采用Pearson线性相关分析法分析CAD、SOX2表达与病变肠段肌间神经丛直径、神经节细胞数的关系。结果HD患儿的狭窄段组织中CAD蛋白、SOX2蛋白阳性表达率分别为7.14%、12.50%,移行段肠组织中CAD蛋白、SOX2蛋白阳性表达率分别为32.14%、35.71%,明显低于对照组的78.26%、82.61%,而狭窄段组织中CAD蛋白、SOX2蛋白阳性表达率明显低于移行段,差异均有统计学意义(P<0.05);HD患儿的狭窄段肠组织中肌间神经丛直径、神经节细胞数目分别为(30.66±5.14)μm、(0.83±0.24)个/视野,移行段肠组织中肌间神经丛直径、神经节细胞数目分别为(31.20±5.83)μm、(1.94±0.56)个/视野,明显短(少)于对照组的(54.29±8.50)μm、(5.40±1.84)个/视野,狭窄段肠组织中肌间神经丛直径、神经节细胞数目明显短(少)于移行段,差异均有统计学意义(P<0.05);Pearson相关分析结果显示,HD患儿狭窄段、移行段肠组织中肌间神经丛直径、神经节细胞数目与CAD蛋白、SOX2蛋白表达强度均呈显著正相关(P<0.05)。结论HD患儿病变结肠组织中CAD蛋白、SOX2蛋白的表达强度下调,可能与肌间神经丛直径、神经节细胞数目的减少有关。 展开更多
关键词 先天性巨结肠 组织蛋白酶d 性别决定区Y框蛋白2 相关性
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PCT、hs-CRP、D-D在评估血流感染严重程度及预后不良中的应用价值 被引量:1
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作者 邹小红 凌利芬 +3 位作者 刘小晴 叶开接 陈盈 吴超 《检验医学与临床》 CAS 2024年第8期1149-1152,1156,共5页
目的探讨炎症指标降钙素原(PCT)、超敏C反应蛋白(hs-CRP)、D-二聚体(D-D)在血流感染严重程度及预后不良中的应用价值。方法选取2019年2月至2021年9月该院收治的254例血流感染患者作为研究对象。根据临床资料将其分为菌血症组(81例)、脓... 目的探讨炎症指标降钙素原(PCT)、超敏C反应蛋白(hs-CRP)、D-二聚体(D-D)在血流感染严重程度及预后不良中的应用价值。方法选取2019年2月至2021年9月该院收治的254例血流感染患者作为研究对象。根据临床资料将其分为菌血症组(81例)、脓毒血症组(109例)、脓毒性休克组(64例)。再根据预后情况将其分为存活组(207例)和死亡组(47例)。检测并比较菌血症组、脓毒血症组和脓毒性休克组,以及存活组和死亡组PCT、hs-CRP及D-D水平。绘制受试者工作特征(ROC)曲线分析PCT、hs-CRP及D-D对血流感染患者预后不良的预测价值。结果脓毒性休克组的PCT、hs-CRP及D-D水平均高于脓毒血症组和菌血症组,且脓毒血症组均高于菌血症组,差异均有统计学意义(P<0.05)。与存活组比较,死亡组中PCT、hs-CRP及D-D水平均明显升高,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,PCT、hs-CRP及D-D单独预测血流感染预后不良的曲线下面积(AUC)分别为0.739、0.769、0.858。PCT联合hs-CRP、hs-CRP联合D-D、PCT联合D-D预测血流感染预后不良的AUC分别为0.787、0.870、0.856。3项指标联合预测血流感染预后不良的AUC为0.870。结论PCT、hs-CRP、D-D可作为预测血流感染患者预后不良的生物标志物,3项指标联合预测血流感染患者预后不良的价值更高。 展开更多
关键词 血流感染 降钙素原 超敏C反应蛋白 d-二聚体 疾病预后
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