芒是麦类作物穂部器官的重要组成部分,在提高籽粒产量、促进种子传播和防御虫害等方面具有重要作用。大麦具有丰富的芒型突变体,加之其二倍体的特性,成为麦类作物芒器官形态建成研究的理想作物。本研究报道了一个大麦芒型突变体材料calc...芒是麦类作物穂部器官的重要组成部分,在提高籽粒产量、促进种子传播和防御虫害等方面具有重要作用。大麦具有丰富的芒型突变体,加之其二倍体的特性,成为麦类作物芒器官形态建成研究的理想作物。本研究报道了一个大麦芒型突变体材料calcaroides,表现为外稃顶端或是稃芒基部异形凸起,形成呈钩状不完全花器结构,属基部钩芒类型。突变体芒较短并伴随抽穗期推迟,株高、穗长和穗粒数显著降低等表型。遗传分析表明,突变体的芒型突变性状受单隐性基因cal-d控制。前期利用cal-d导入系BW106×Bowman的F_(2)群体,结合简化基因组测序(GBS,genotyping by sequencing)分析,将cal-d基因初步定位于3H染色体。进一步利用来自F_(2)的杂合单株,包括13000株单株的F_(2:3)群体进行精细定位,最终将cal-d基因定位于3H染色体153~329 Mb区间的近着丝粒区域。通过转录组混池测序分析结合大麦基因组和表达谱资源数据库,初步筛选了9个候选基因。本研究结果为大麦芒型突变基因cal-d的克隆与功能验证奠定了基础,对于解析麦类作物芒的遗传发育机制具有重要的意义。展开更多
目的观察DHS内固定联合药物治疗骨质疏松性股骨粗隆间骨折的临床疗效。方法对46例患者随机采用DHS内固定术手术治疗(对照组)或DHS配合鲑鱼降钙素喷鼻剂加服钙尔奇D片治疗(治疗组),观察比较两组患者在术后4、8、12周末X线表现,6个月后两...目的观察DHS内固定联合药物治疗骨质疏松性股骨粗隆间骨折的临床疗效。方法对46例患者随机采用DHS内固定术手术治疗(对照组)或DHS配合鲑鱼降钙素喷鼻剂加服钙尔奇D片治疗(治疗组),观察比较两组患者在术后4、8、12周末X线表现,6个月后两组患者治疗前后患侧股骨近端(bone mineral density,BMD)值变化和术后疗效。其中治疗组26例,对照组20例,两组在性别、年龄、骨折类型等一般情况方面都具有可比性。结果治疗组患者的骨痂、骨质生成的时间均较对照组明显缩短,生成数量增加,治疗效果明显。治疗6个月后,治疗组治疗后比治疗前患侧股骨近端BMD有所增加(P<0.05),而对照组无明显差异(P>0.05),且治疗组术后疗效的优良率高于对照组(P<0.05),差异有统计学意义。结论DHS内固定联合药物治疗骨质疏松性股骨粗隆间骨折能有效促进骨痂的生长,加速骨折的愈合,减少了患者的卧床时间,促进了早期功能锻炼和患髋的功能恢复。展开更多
Using nystatin-perforated whole-cell recording configuration, the modulatory effect of N-methyl-D-aspartate (NMDA) on γ-aminobutyric acid (GABA)-activated whole-cell currents was investigated in neurons freshly disso...Using nystatin-perforated whole-cell recording configuration, the modulatory effect of N-methyl-D-aspartate (NMDA) on γ-aminobutyric acid (GABA)-activated whole-cell currents was investigated in neurons freshly dissociated from the rat sacral dorsal commissural nucleus (SDCN). The results showed that: (i) NMDA suppressed GABA- and muscimol (Mus)-activated currents (IGABA and IMUS), respectively in the Mg2+-free external solution containing 1 μmol/L glycine at a holding potential (VH) of -40 mV in SDCN neurons. The selective NMDA receptor antagonist, D-2-amino-5-phosphonovaleric acid (APV, 100 μmol/L), inhibited the NMDA-evoked currents and blocked the NMDA-induced suppression of IGABA; (ii) when the neurons were incubated in a Ca2+-free bath or pre-loaded with a membrane-permeable Ca2+ chelator, BAPTA AM (10 nmol/L), the inhibitory effect of NMDA on IGABA disappeared. Cd2+ (10 μmol/L) or La3+(30 μmol/L), the non-selective blockers of voltage-dependent calcium channels, did not affect the suppression of IGABA by NMDA application; (iii) the suppression of IGABA by NMDA was inhibited by KN-62, a cal-cium/calmodulin-dependent protein kinase II (CaMKII) inhibitor. These results indicated that the inhibition of GABA response by NMDA is Ca2+-dependent and CaMKII is involved in the process of the Ca2+-dependent inhibition.展开更多
文摘芒是麦类作物穂部器官的重要组成部分,在提高籽粒产量、促进种子传播和防御虫害等方面具有重要作用。大麦具有丰富的芒型突变体,加之其二倍体的特性,成为麦类作物芒器官形态建成研究的理想作物。本研究报道了一个大麦芒型突变体材料calcaroides,表现为外稃顶端或是稃芒基部异形凸起,形成呈钩状不完全花器结构,属基部钩芒类型。突变体芒较短并伴随抽穗期推迟,株高、穗长和穗粒数显著降低等表型。遗传分析表明,突变体的芒型突变性状受单隐性基因cal-d控制。前期利用cal-d导入系BW106×Bowman的F_(2)群体,结合简化基因组测序(GBS,genotyping by sequencing)分析,将cal-d基因初步定位于3H染色体。进一步利用来自F_(2)的杂合单株,包括13000株单株的F_(2:3)群体进行精细定位,最终将cal-d基因定位于3H染色体153~329 Mb区间的近着丝粒区域。通过转录组混池测序分析结合大麦基因组和表达谱资源数据库,初步筛选了9个候选基因。本研究结果为大麦芒型突变基因cal-d的克隆与功能验证奠定了基础,对于解析麦类作物芒的遗传发育机制具有重要的意义。
文摘目的观察DHS内固定联合药物治疗骨质疏松性股骨粗隆间骨折的临床疗效。方法对46例患者随机采用DHS内固定术手术治疗(对照组)或DHS配合鲑鱼降钙素喷鼻剂加服钙尔奇D片治疗(治疗组),观察比较两组患者在术后4、8、12周末X线表现,6个月后两组患者治疗前后患侧股骨近端(bone mineral density,BMD)值变化和术后疗效。其中治疗组26例,对照组20例,两组在性别、年龄、骨折类型等一般情况方面都具有可比性。结果治疗组患者的骨痂、骨质生成的时间均较对照组明显缩短,生成数量增加,治疗效果明显。治疗6个月后,治疗组治疗后比治疗前患侧股骨近端BMD有所增加(P<0.05),而对照组无明显差异(P>0.05),且治疗组术后疗效的优良率高于对照组(P<0.05),差异有统计学意义。结论DHS内固定联合药物治疗骨质疏松性股骨粗隆间骨折能有效促进骨痂的生长,加速骨折的愈合,减少了患者的卧床时间,促进了早期功能锻炼和患髋的功能恢复。
文摘Using nystatin-perforated whole-cell recording configuration, the modulatory effect of N-methyl-D-aspartate (NMDA) on γ-aminobutyric acid (GABA)-activated whole-cell currents was investigated in neurons freshly dissociated from the rat sacral dorsal commissural nucleus (SDCN). The results showed that: (i) NMDA suppressed GABA- and muscimol (Mus)-activated currents (IGABA and IMUS), respectively in the Mg2+-free external solution containing 1 μmol/L glycine at a holding potential (VH) of -40 mV in SDCN neurons. The selective NMDA receptor antagonist, D-2-amino-5-phosphonovaleric acid (APV, 100 μmol/L), inhibited the NMDA-evoked currents and blocked the NMDA-induced suppression of IGABA; (ii) when the neurons were incubated in a Ca2+-free bath or pre-loaded with a membrane-permeable Ca2+ chelator, BAPTA AM (10 nmol/L), the inhibitory effect of NMDA on IGABA disappeared. Cd2+ (10 μmol/L) or La3+(30 μmol/L), the non-selective blockers of voltage-dependent calcium channels, did not affect the suppression of IGABA by NMDA application; (iii) the suppression of IGABA by NMDA was inhibited by KN-62, a cal-cium/calmodulin-dependent protein kinase II (CaMKII) inhibitor. These results indicated that the inhibition of GABA response by NMDA is Ca2+-dependent and CaMKII is involved in the process of the Ca2+-dependent inhibition.
