目的:研究NDRG2基因与去泛素化酶Zc3h12d在NF-κB转导通路中的调控机制。方法:采用LPS刺激人支气管气道上皮16HBE细胞株来构建炎性刺激时气道黏液高分泌模型,用瞬时转染过表达NDRG2基因和Zc3h12d si RNA干扰下调16HBE细胞,将细胞分成LPS...目的:研究NDRG2基因与去泛素化酶Zc3h12d在NF-κB转导通路中的调控机制。方法:采用LPS刺激人支气管气道上皮16HBE细胞株来构建炎性刺激时气道黏液高分泌模型,用瞬时转染过表达NDRG2基因和Zc3h12d si RNA干扰下调16HBE细胞,将细胞分成LPS+瞬时转染过表达NDRG2组(A组)、LPS+瞬时转染过表达NDRG2+Zc3h12d si RNA组(B组)、LPS+瞬时转染Zc3h12d si RNA组(C组)、LPS+转染空白载体组(D组),以未做任何处理的16HBE细胞作为对照组(E组)。反转录PCR检测气道黏蛋白(mucin,MUC)5AC m RNA的表达水平,ELISA法检测IL-1β、IL-6等炎症因子及MUC5AC的分泌水平,Western印迹检测Zc3h12d和NF-κB p65的分泌水平,激光共聚焦法检测16HBE细胞内MUC5AC表达。结果:与B组比较,A组炎症因子及MUC5AC表达明显减少,差异有统计学意义(P<0.05);而B组与C组比较,差异无统计学意义(P>0.05)。与D组比较,A组炎症因子及MUC5AC的表达明显降低,差异均有统计学意义(均P<0.05)。D组与E组比较,LPS刺激后炎症因子及MUC5AC分泌增多,差异均有统计学意义(均P<0.05)。结论:NDRG2基因可以通过Zc3h12d而实现对NF-κB转导通路的调控。展开更多
The molecule with Th symmetry is rare.A dodecahedrane-like molecule C12H12B8 with uncommon Th symmetry has been reported here.Density functional calculations and minimization techniques have been employed to character...The molecule with Th symmetry is rare.A dodecahedrane-like molecule C12H12B8 with uncommon Th symmetry has been reported here.Density functional calculations and minimization techniques have been employed to characterize its structural and electronic properties.Its geometry,electronic properties,vibrational frequencies and heat of formation have been calculated at the B3LYP/6-311＋G（d,p） level of theory.The absence of imaginary vibrational frequency confirms that it corresponds to true minimum on the potential energy hypersurface.Its vibrational bands in the IR intensity have been discussed and compared with future experimental identification.At the B3LYP/6-311＋G（d,p） level,the heat of formation has been calculated to be 720.9 kJ mol^-1 using the isodesmic reaction.According to this value,it is a potential high energy density molecule.展开更多
A coplanar dicage pentaprismane molecule C16H12 has been studied by using DFT at B3LYP/D95* level and ab initio method at MP2/D95* level, and the vibrational frequencies of the molecule have been calculated at B3LYP/D...A coplanar dicage pentaprismane molecule C16H12 has been studied by using DFT at B3LYP/D95* level and ab initio method at MP2/D95* level, and the vibrational frequencies of the molecule have been calculated at B3LYP/D95* level too. The results show that the molecule is thermodynamically stable. Some physico-chemical properties, such as geometry, simulated IR spectrum and heat of formation, have been reported.展开更多
文摘目的:研究NDRG2基因与去泛素化酶Zc3h12d在NF-κB转导通路中的调控机制。方法:采用LPS刺激人支气管气道上皮16HBE细胞株来构建炎性刺激时气道黏液高分泌模型,用瞬时转染过表达NDRG2基因和Zc3h12d si RNA干扰下调16HBE细胞,将细胞分成LPS+瞬时转染过表达NDRG2组(A组)、LPS+瞬时转染过表达NDRG2+Zc3h12d si RNA组(B组)、LPS+瞬时转染Zc3h12d si RNA组(C组)、LPS+转染空白载体组(D组),以未做任何处理的16HBE细胞作为对照组(E组)。反转录PCR检测气道黏蛋白(mucin,MUC)5AC m RNA的表达水平,ELISA法检测IL-1β、IL-6等炎症因子及MUC5AC的分泌水平,Western印迹检测Zc3h12d和NF-κB p65的分泌水平,激光共聚焦法检测16HBE细胞内MUC5AC表达。结果:与B组比较,A组炎症因子及MUC5AC表达明显减少,差异有统计学意义(P<0.05);而B组与C组比较,差异无统计学意义(P>0.05)。与D组比较,A组炎症因子及MUC5AC的表达明显降低,差异均有统计学意义(均P<0.05)。D组与E组比较,LPS刺激后炎症因子及MUC5AC分泌增多,差异均有统计学意义(均P<0.05)。结论:NDRG2基因可以通过Zc3h12d而实现对NF-κB转导通路的调控。
基金supported by the Natural Science Foundation of Shandong Province (No. ZR2011BM022)
文摘The molecule with Th symmetry is rare.A dodecahedrane-like molecule C12H12B8 with uncommon Th symmetry has been reported here.Density functional calculations and minimization techniques have been employed to characterize its structural and electronic properties.Its geometry,electronic properties,vibrational frequencies and heat of formation have been calculated at the B3LYP/6-311＋G（d,p） level of theory.The absence of imaginary vibrational frequency confirms that it corresponds to true minimum on the potential energy hypersurface.Its vibrational bands in the IR intensity have been discussed and compared with future experimental identification.At the B3LYP/6-311＋G（d,p） level,the heat of formation has been calculated to be 720.9 kJ mol^-1 using the isodesmic reaction.According to this value,it is a potential high energy density molecule.
基金by NNSFC (60071006) and the Natural Science Foundation of Shandong Province (Y2002G11)
文摘A coplanar dicage pentaprismane molecule C16H12 has been studied by using DFT at B3LYP/D95* level and ab initio method at MP2/D95* level, and the vibrational frequencies of the molecule have been calculated at B3LYP/D95* level too. The results show that the molecule is thermodynamically stable. Some physico-chemical properties, such as geometry, simulated IR spectrum and heat of formation, have been reported.
