目的研究人类剪切蛋白(drosophila behavior human splicing,DBHS)家族基因,包括p5nrb、PSF、PSPC1基因,对食管鳞状细胞癌(以下简称食管磷癌)细胞系TE-8细胞系迁移、侵袭能力的影响。方法构建p54nrb、PSF、PSPC1基因过表达载体,通过脂...目的研究人类剪切蛋白(drosophila behavior human splicing,DBHS)家族基因,包括p5nrb、PSF、PSPC1基因,对食管鳞状细胞癌(以下简称食管磷癌)细胞系TE-8细胞系迁移、侵袭能力的影响。方法构建p54nrb、PSF、PSPC1基因过表达载体,通过脂质体法转染TE-8细胞,转染48 h后qRT-PCR和Western blotting检测各组细胞p54nrb、PSF、PSPC1在mRNA和蛋白质水平的表达。细胞划痕实验及覆盖有Matrigel的Transwell小室检测食管鳞癌细胞的迁移、侵袭能力。结果过表达p54nrb、PSF、PSPC1后,qRT-PCR和Western blotting检测证实食管鳞癌TE-8细胞中上述基因表达在mRNA水平和蛋白质水平均明显上调,细胞划痕实验及Transwell小室实验证实食管鳞癌TE-8细胞过表达p54nrb、PSF、PSPC1后,其迁移、侵袭能力增强。结论过表达DBHS家族基因可促进食管鳞癌细胞的迁移侵袭。展开更多
目的研究多巴胺β羟化酶(DBH)基因启动子区的19 bp Insert/Delet位点和α-2B肾上腺素能受体(α-2BAR)基因12Glu9位点与原发性高血压的相关性。方法选取原发性高血压患者196例,健康体检者121例,检测DBH基因启动子区的19 bp Insert/Delet...目的研究多巴胺β羟化酶(DBH)基因启动子区的19 bp Insert/Delet位点和α-2B肾上腺素能受体(α-2BAR)基因12Glu9位点与原发性高血压的相关性。方法选取原发性高血压患者196例,健康体检者121例,检测DBH基因启动子区的19 bp Insert/Delet位点和α-2BAR基因12Glu9位点多态性。结果 19 bp Insert/Delet位点和12Glu9位点的基因型和等位基因频率在病例组与对照组差异没有统计学意义(P>0.05);19 bpInsert/Delet位点和12Glu9位点的基因型对收缩压、舒张压、Na+浓度、体质量指数、K+浓度和C1-浓度变化的影响差异无统计学意义(P>0.05)。结论 DBH基因和α-2BAR基因在该人群中可能与原发性高血压和有关离子浓度变化无关。展开更多
The number and composition of species in a community can be quantified withα-diversity indices,including species richness(R),Simpson’s index(D),and the Shannon-Wiener index(H΄).In forest communities,there are large ...The number and composition of species in a community can be quantified withα-diversity indices,including species richness(R),Simpson’s index(D),and the Shannon-Wiener index(H΄).In forest communities,there are large variations in tree size among species and individu-als of the same species,which result in differences in eco-logical processes and ecosystem functions.However,tree size inequality(TSI)has been largely neglected in studies using the available diversity indices.The TSI in the diameter at breast height(DBH)data for each of 99920 m×20 m forest census quadrats was quantified using the Gini index(GI),a measure of the inequality of size distribution.The generalized performance equation was used to describe the rotated and right-shifted Lorenz curve of the cumulative proportion of DBH and the cumulative proportion of number of trees per quadrat.We also examined the relationships ofα-diversity indices with the GI using correlation tests.The generalized performance equation effectively described the rotated and right-shifted Lorenz curve of DBH distributions,with most root-mean-square errors(990 out of 999 quadrats)being<0.0030.There were significant positive correlations between each of threeα-diversity indices(i.e.,R,D,and H’)and the GI.Nevertheless,the total abundance of trees in each quadrat did not significantly influence the GI.This means that the TSI increased with increasing spe-cies diversity.Thus,two new indices are proposed that can balanceα-diversity against the extent of TSI in the com-munity:(1−GI)×D,and(1−GI)×H’.These new indices were significantly correlated with the original D and H΄,and did not increase the extent of variation within each group of indices.This study presents a useful tool for quantifying both species diversity and the variation in tree sizes in forest communities,especially in the face of cumulative species loss under global climate change.展开更多
文摘目的研究多巴胺β羟化酶(DBH)基因启动子区的19 bp Insert/Delet位点和α-2B肾上腺素能受体(α-2BAR)基因12Glu9位点与原发性高血压的相关性。方法选取原发性高血压患者196例,健康体检者121例,检测DBH基因启动子区的19 bp Insert/Delet位点和α-2BAR基因12Glu9位点多态性。结果 19 bp Insert/Delet位点和12Glu9位点的基因型和等位基因频率在病例组与对照组差异没有统计学意义(P>0.05);19 bpInsert/Delet位点和12Glu9位点的基因型对收缩压、舒张压、Na+浓度、体质量指数、K+浓度和C1-浓度变化的影响差异无统计学意义(P>0.05)。结论 DBH基因和α-2BAR基因在该人群中可能与原发性高血压和有关离子浓度变化无关。
基金supported by the National Natural Science Foundation of China(32101260).
文摘The number and composition of species in a community can be quantified withα-diversity indices,including species richness(R),Simpson’s index(D),and the Shannon-Wiener index(H΄).In forest communities,there are large variations in tree size among species and individu-als of the same species,which result in differences in eco-logical processes and ecosystem functions.However,tree size inequality(TSI)has been largely neglected in studies using the available diversity indices.The TSI in the diameter at breast height(DBH)data for each of 99920 m×20 m forest census quadrats was quantified using the Gini index(GI),a measure of the inequality of size distribution.The generalized performance equation was used to describe the rotated and right-shifted Lorenz curve of the cumulative proportion of DBH and the cumulative proportion of number of trees per quadrat.We also examined the relationships ofα-diversity indices with the GI using correlation tests.The generalized performance equation effectively described the rotated and right-shifted Lorenz curve of DBH distributions,with most root-mean-square errors(990 out of 999 quadrats)being<0.0030.There were significant positive correlations between each of threeα-diversity indices(i.e.,R,D,and H’)and the GI.Nevertheless,the total abundance of trees in each quadrat did not significantly influence the GI.This means that the TSI increased with increasing spe-cies diversity.Thus,two new indices are proposed that can balanceα-diversity against the extent of TSI in the com-munity:(1−GI)×D,and(1−GI)×H’.These new indices were significantly correlated with the original D and H΄,and did not increase the extent of variation within each group of indices.This study presents a useful tool for quantifying both species diversity and the variation in tree sizes in forest communities,especially in the face of cumulative species loss under global climate change.
