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Purification of a Choleragenoid by Ion-exchange Chromatography
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作者 林厚怡 宋兰珍 佘雪轩 《The Journal of Biomedical Research》 CAS 1994年第1期22-25,共4页
Abstract: Choleragenoid was obtained in pure form by ultra-filteration and fractionation on cationexchange resin-phospho-cellulose column. The choleragenoid was highly pure as judged by the electrophoresis of isoelect... Abstract: Choleragenoid was obtained in pure form by ultra-filteration and fractionation on cationexchange resin-phospho-cellulose column. The choleragenoid was highly pure as judged by the electrophoresis of isoelectric focusing,immunization and SDS-gel electrophoresis.The results of test are thesame as that of the standard choleragenoid.Keywoeds:choleragenoid; vibrio cholerae; purification;ion-exchange; chromatography 展开更多
关键词 chromatography Choleragenoid ion-exchange PURIFICATION by of
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Modified DIX model for ion-exchange equilibrium of L-phenylalanine on a strong cation-exchange resin 被引量:3
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作者 Jinglan wu Pengfei Jiao +2 位作者 Wei Zhuang Jingwei Zhou Hanjie Ying 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2016年第10期1386-1391,共6页
L-phenylalanine, one of the nine essential amino acids for the human body, is extensively used as an ingredient in food, pharmaceutical and nutrition industries. A suitable equilibrium model is required for purificati... L-phenylalanine, one of the nine essential amino acids for the human body, is extensively used as an ingredient in food, pharmaceutical and nutrition industries. A suitable equilibrium model is required for purification of L-phenylalanine based on ion-exchange chromatography. In this work, the equilibrium uptake of L-phenylalanine on a strong acid-cation exchanger SH11 was investigated experimentally and theoretically. A modified Donnan ion-exchange (DIX) model, which takes the activiW into account, was established to predict the uptake of L-phenylalanine at various solution pH values. The model parameters including selectivity and mean activity coefficient in the resin phase are presented. The modified DIX model is in good agreement with the experimental data. The optimum operating pH value of 2.0, with the highest t-phenylalanine uptake on the resin, is predicted by the model. This basic information combined with the general mass transfer model will lay the foundation for the prediction of dynamic behavior of fixed bed separation process. 展开更多
关键词 ion-exchange equilibrium L-phenylalanine Mathematical modeling chromatography Mean ionic activity coefficient
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Suitability of Steric Mass-action Model for Ion-exchange Equilibrium of Micromolecule
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作者 陈卫东 王艳东 苏志国 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2005年第1期18-23,共6页
The steric mass-action (SMA) model has been widely reported in the literature for ion-exchange and metal-affinity interaction adsorption equilibrium of biomacromolecules. In this paper, the usefulness of SMA model is ... The steric mass-action (SMA) model has been widely reported in the literature for ion-exchange and metal-affinity interaction adsorption equilibrium of biomacromolecules. In this paper, the usefulness of SMA model is analyzed for describing micromolecule ion-exchange equilibrium onto cation exchangers, CM Sephadex C-25 and Streamline SP. Batch adsorption experiments with ephedrine hydrochloride as a model adsorbate are carried out to determine the model parameters, that is, steric factor, characteristic charge and equilibrium constant. The result shows that the SMA model parameters of micromolecule cannot be obtained using the nonlinear least-square fitting method as protein's due to the remarkable difference between the molecular mass and dimension of micromolecule and protein. It is considered that the small size of the adsorbates dealt with in this study justifies the neglect of steric hindrances arising from adsorbate bulkiness. Thus, the three-parameter SMA model is reduced to two-parameter one (i.e., steric factor is equal to zero) for describing micromolecule ion-exchange equilibrium. It is found that the equilibrium constant for CM Sephadex C-25 increases with increasing ionic strength, while the equilibrium constant for Streamline SP shows an opposite trend. This is probably due to the remarkable difference between the physicalpro perties of the two adsorbents. Then, the relationship between the equilibrium constant and ionic strength is described by an expression. The computer simulations show that, the theoretical model with the correlation is promising in the prediction of micromolecule adsorption decrease with increasing ionic strength in a wide range of salt concentration. 展开更多
关键词 micromolecule ion-exchange equilibrium steric mass-action model chromatography
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Obtaining Bacteriocins by Chromatographic Methods 被引量:1
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作者 Valery Borzenkov Vladimir Surovtsev Ivan Dyatlov 《Advances in Bioscience and Biotechnology》 2014年第5期446-451,共6页
Bacteriocins are a large group of chromosome or plasmid-encoded and ribosomally synthesized low-molecular-weight (2 to 6 kDa) antimicrobial and amphiphilous peptides produced by Gr+ or Gr- bacteria [1]. Their low toxi... Bacteriocins are a large group of chromosome or plasmid-encoded and ribosomally synthesized low-molecular-weight (2 to 6 kDa) antimicrobial and amphiphilous peptides produced by Gr+ or Gr- bacteria [1]. Their low toxicity as well as the absence of allergenicity and reactogenicity is confirmed by testing selected bacteriocins [2] [3]. Bacteriocins can be widely used as preservatives and antibiotic alternatives in medicine. Nisin, a Streptococcus lactis-derived bacteriocin, has been in practice in food industry for a long time. A relevant product contains about 2.5% of nisin. For medical use (e.g., when injected into the blood stream), highly purified drugs are required. However, the yield of bacteriocins accounts for no more than a few percents from the total activity in the culture liquid. In this paper, we propose methods (by example of two B. subtilis strains), allowing to increase the yield up to ~80%. It is believed that other bacteriocins may be purified by these methods and with the same yield. 展开更多
关键词 PURIFICATION BACTERIOCINS Surface Cell FRAGMENTS HYDROPHOBICITY Membranes ion-exchange HYDROPHOBIC chromatography
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Large_scale Isolation and Purification of R_phycoerythrin from Red Alga Palmaria palmata Using the Expanded Bed Adsorption Method 被引量:2
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作者 王广策 孙海宝 +1 位作者 范晓 曾呈奎 《Acta Botanica Sinica》 CSCD 2002年第5期541-546,共6页
R-phycoerythrin, a light-harvesting protein in some marine algae, and can be widely used in medicine, was isolated and purified from a red alga, Palmaria palmata (Lannaeus) Kuntze, using the streamline column (expande... R-phycoerythrin, a light-harvesting protein in some marine algae, and can be widely used in medicine, was isolated and purified from a red alga, Palmaria palmata (Lannaeus) Kuntze, using the streamline column (expanded bed adsorption) combined with ion-exchange chromatography. Because the crude extract was applied to the column upwardly, the column would not be blocked by polysaccharides usually very abundant in the extract of marine alga, this kind of blockage could hardly lie overcome in ordinary chromatographic column. After applying the crude extract containing 0.5 mol/L (NH4)(2)SO4, (NH4)(2)SO4 solution of different concentrations (0.2 mol/L, 0.1 mol/L and 0.05 mol/L) was used to elute the column downwardly and the eluates were collected and desalted. The desalted eluates were then applied onto all ion-exchange chromatographic column loaded with Q-sepharose for further purification of the R-phycoerythrin. Through these two steps, the purity (OD565/OD280) of the R-phycoerythrin from P. palmata was up to 3.5, more than 3.2, the commonly accepted criterion for purity, and the yield of the purified R-phycoerythrin could reach 0.122 mg/g of frozen P. palmata, much higher than that of phycobiliproteins purified with the previous methods. The result indicated that the cost of R-phycoerythrin will drop down with the method reported in this article. 展开更多
关键词 Palmaria palmata ( Lannaeus) Kuntze R-PHYCOERYTHRIN streamline column ion-exchange chromatography
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Purification and characterization of acetylcholinesterase from brain tissues of Oreochromis aurea and its application in environmental pesticide monitoring 被引量:1
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作者 YunHua Ding XiaoMin Wu JunBin Fang 《Research in Cold and Arid Regions》 2011年第4期339-343,共5页
Acetylcholinesterase (ACHE) plays an important role in enzyme-based detection of pesticides in the environment. In this paper, ACHE from the Triton X-100 extract of brain tissues of Oreochromis aurea was purified by... Acetylcholinesterase (ACHE) plays an important role in enzyme-based detection of pesticides in the environment. In this paper, ACHE from the Triton X-100 extract of brain tissues of Oreochromis aurea was purified by (NH4)2SO4 fractional precipitation, Sephadex G-100 gel filtration, and DEAE-cellulose ion exchange chromatography. Certain biochemical characterizations of the purified enzyme and inhibition of pesticides on the enzyme were also studied. The specific activity of this purified enzyme was 20.628 U/mg protein, fold of purification was 139, and recovery was 22.1%. The optimal temperature of this enzyme was between 35-40 ℃, and optimal pH was between 7.5-8.0. The Michaelis constant (Kin) for acetylthiocholine iodide was 0.183 mmol/L. The enzyme activity was inhibited by excess substrate, and optimal substrate concentration was 6 mmol/L. Four pesticides (dichlorvos, phoxim, triazophos, and methomyl) exhibited strong inhibitions on this enzyme with IC50 less than 5 μg/mL. This study suggests that Oreochromis aurea (tilapia) could be a good enzyme source for pesticide monitoring in water environments. 展开更多
关键词 Oreochromis aurea ACETYLCHOLINESTERASE PURIFICATION gel filtration ion-exchange chromatography pesticide sensitivity
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Isolation and Identification of Ectromelia Virus from Suspected Mice
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作者 Lin Houyi Song Lanzheng She Xuexuan(Department of Biochemisty,Nanjing Medical University, Nanjing 210029, P. R. China)(Derpatment of Microbiology,Nanjing Medical University, Nanjing 210029, P- R.China) 《The Journal of Biomedical Research》 CAS 1995年第1期28-28,共1页
Choleragenoid was obtained in pure form by ultra-filteration and fractionation on cationexchange resin-phospho-cellulose column. The choleragenoid was highly pure as judged by the electrophoresis of isoelectric focusi... Choleragenoid was obtained in pure form by ultra-filteration and fractionation on cationexchange resin-phospho-cellulose column. The choleragenoid was highly pure as judged by the electrophoresis of isoelectric focusing,immunization and SDS-gel electrophoresis.The results of test are thesame as that of the standard choleragenoid. 展开更多
关键词 choleragenoid vibrio cholerae PURIFICATION ion-exchange chromatography
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异丙肾上腺素在硅酸铋离子交换薄层上的选择性分离与测定(英文)
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作者 Vanik GHOULIPOUR Zahra HASSANKHANI-MAJD 《色谱》 CAS CSCD 北大核心 2015年第6期667-671,共5页
A simple and sensitive method for the separation and determination of isoproterenol from other doping drugs has been developed on thin layers of bismuth silicate,a synthetic inorganic ion exchanger as adsorbent in thi... A simple and sensitive method for the separation and determination of isoproterenol from other doping drugs has been developed on thin layers of bismuth silicate,a synthetic inorganic ion exchanger as adsorbent in thin layer chromatography(TLC).A mixture of methanol and 0.1 mol/L formic acid(3∶7,v/v)was employed as the mobile phase.The development time was 32 min.The quantitative measurement were performed with a Camag TLC Scanner-3 at wavelength(λ)of 410 nm.The isoproterenol recovery in this procedure was 98.9%.The linear correlation coefficient was greater than 0.987 1 and the relative standard deviation(RSD)was less than 0.94.The limit of detection(LOD)and limit of quantification(LOQ)were 7.7×10-7mol/L and 3.85×10-6mol/L,respectively.This method has been applied in the determination of isoproterenol in dosage forms and in biological fluids. 展开更多
关键词 high performance thin layer chromatography(HPTLC) inorganic ion-exchanger DENSITOMETRY
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Separation characteristics of boron isotopes in continuous annular chromatography
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作者 Lihua Zhou Kejing Wu +3 位作者 Wei Qin Guanchun Wang Daogui Fu Weiyang Fei 《Science China Chemistry》 SCIE EI CAS CSCD 2015年第7期1187-1192,共6页
A well designed continuous annular chromatograph (CAC) column was set to stndy the enrichment of Boron isotopes. The column is 820 mm high and consists of a rotational annulus with an outer diameter of 246 mm and a ... A well designed continuous annular chromatograph (CAC) column was set to stndy the enrichment of Boron isotopes. The column is 820 mm high and consists of a rotational annulus with an outer diameter of 246 mm and a width of 15 mm. The weak basic resin (Diaion WA21J), the boric acid and water were used as the absorbent, the feed and the eluent respectively. The effects of CAC rotating speed, concentration of boric acid and flow ratio of eluent to feed on the enrichment of 10B were investigated. The concentration of boron isotope was determined by a Thermal Elemental X7 ICP-MS (Thermo Electron Co., USA). It is shown from the experimental results that 10B and 11B can be separated effectively by this CAC column. By fitting the experimental elution profiles based on reliable mathematic models and software the mass transfer coefficient k and adsorp- tion equilibrium constant K values of 10B and 11B in CAC column were estimated. It is clear from this study that the CAC has practical continuous operational capabilities in comparison with fixed batch chromatography and is an effective technology for seoarating boron isotones. 展开更多
关键词 continuous annular chromatography boron isotopes separation ion-exchange mathematic model
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Human cyclophilin 33 (hCyP33) in T-cell binds specifically to poly(A)^+RNA (mRNA) 被引量:1
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作者 张万起 袁直 +3 位作者 宓怀风 元云峰 何炳林 王亦农 《Science China Chemistry》 SCIE EI CAS 2002年第3期275-281,共7页
Human cyclophilin 33 (hCyP33), found in 1996, consists of an RNA-binding domain in N-terminus, a cyclophilin domain in C-terminus and a connected part between the two domains. RNA-binding proteins concern functions, s... Human cyclophilin 33 (hCyP33), found in 1996, consists of an RNA-binding domain in N-terminus, a cyclophilin domain in C-terminus and a connected part between the two domains. RNA-binding proteins concern functions, such as splicing, modification and transport, after transcription in eukaryotic cells. Cyclophilins (CyPs) possess enzymatic activity, namely peptidyl-proryl cis-trans isomerase (PPIase). They are involved in folding, transport and interaction of proteins. Cyclosporin A (CsA), an immunosuppressant used by organ transplantation, binds to CyPs and suppresses their enzymatic activity. However, up to now it is unknown that which cellular and physiological roles hCyP33, which possesses the above-mentioned both functions, plays. In this paper the binding specificity of hCyP33 to different cellular RNA is investigated by means of ion-exchange chromatography and affinity adsorption. The results show that it binds specifically to poly(A) tailed mRNA, namely poly(A)+RNA. 展开更多
关键词 HUMAN CYCLOPHILIN 33 (hCyP33) ion-exchange chromatography RNA-BINDING specificity.
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