We describe for the first time the synthesis and the optimal conditions for proteinlabeling with a new fluorescent probe,5-chlorosulfoyl-2-thenoyltrifluoroacetone(CTTA),whicb forms ahighly fluorescent conplex with E...We describe for the first time the synthesis and the optimal conditions for proteinlabeling with a new fluorescent probe,5-chlorosulfoyl-2-thenoyltrifluoroacetone(CTTA),whicb forms ahighly fluorescent conplex with Eu<sup>3+</sup> when conjugated to protein.The labeled proteins werecharacterized by absorbance and fluorescence measurements and the effect of labeling on thebiological activity of sone proteins was also studied.It is shown that the new label is suitablefor applications in time-resolved fluoroimmunoassay.展开更多
基金Supported by National Natural Science Foundation of China.
文摘We describe for the first time the synthesis and the optimal conditions for proteinlabeling with a new fluorescent probe,5-chlorosulfoyl-2-thenoyltrifluoroacetone(CTTA),whicb forms ahighly fluorescent conplex with Eu<sup>3+</sup> when conjugated to protein.The labeled proteins werecharacterized by absorbance and fluorescence measurements and the effect of labeling on thebiological activity of sone proteins was also studied.It is shown that the new label is suitablefor applications in time-resolved fluoroimmunoassay.