Background: Renal osteodystrophy (ROD) is a bone disorder resulting from chronic kidney disease (CKD) and related metabolic diseases. Dickkopf-related protein-1 (DKK-1) is critical in regulating bone biology. This stu...Background: Renal osteodystrophy (ROD) is a bone disorder resulting from chronic kidney disease (CKD) and related metabolic diseases. Dickkopf-related protein-1 (DKK-1) is critical in regulating bone biology. This study aimed to evaluate the serum DKK-1 level as a bone marker in children with CKD who undergo regular hemodialysis (HD). Subjects and Methods: This case-control study involved 40 children with CKD on HD and 40 healthy children as controls. The study measured serum DKK-1 levels and performed a dual-energy X-ray absorptiometry scan (DEXA) in line with routine laboratory investigations. Results: There was a significant increase in the serum level of DKK-1 in the patient group compared to the control group. The DKK-1 levels were 2540.65 (2215.4 - 2909.2) pg/ml and 1110.45 (885.45 - 1527.65) pg/ml, respectively, with a p-value of less than 0.001. In the hemodialysis group, 25 patients (62.5%) had low bone mineral density (BMD) with a Z-score of under -2.0. Eighteen of these patients had low BMD in both the neck of the femur and lumbar spines. Additionally, there was a significant increase in serum DKK-1 level in patients with low BMD (2567.35 (2303.8 - 3108.1) pg/ml) compared to patients with normal BMD (2454 (1859 - 2820) pg/ml) (p = 0.041). There was also a significant positive correlation between DKK1 level and phosphorus, alkaline phosphatase, and Parathormone serum levels. In conclusion, the study indicates a clear correlation between DKK-1 and BMD in children undergoing maintenance hemodialysis. DKK1 is a promising biomarker for CKD-MBD.展开更多
目的探讨Wnt-1、Dickkopf相关蛋白1(DKK-1)、红细胞分布宽度(red cell distribution width,RDW)与冠心病的相关性,并分析Wnt-1、DKK-1和RDW之间的内在联系及其对冠心病的联合诊断价值。方法选取行冠状动脉(简称冠脉)造影的住院患者188例...目的探讨Wnt-1、Dickkopf相关蛋白1(DKK-1)、红细胞分布宽度(red cell distribution width,RDW)与冠心病的相关性,并分析Wnt-1、DKK-1和RDW之间的内在联系及其对冠心病的联合诊断价值。方法选取行冠状动脉(简称冠脉)造影的住院患者188例,根据冠脉造影结果将其分为冠心病组(n=128)和非冠心病组(n=60)并收集临床资料,比较两组间的Wnt-1、DKK-1、RDW差异。结果冠心病组患者血清Wnt-1水平降低,而DKK-1、RDW升高,与非冠心病组相比差异均有统计学意义(P<0.05);冠心病组中的急性心肌梗死组(30例)、不稳定型心绞痛组(54例)和稳定型心绞痛组(44例)三个亚组间Wnt-1、DKK-1及RDW差异均无统计学意义(P>0.05)。经Logistic回归分析,Wnt-1、DKK-1、RDW的OR值分别为0.947、1.023、64.995。三者联合诊断冠心病的受试者工作特征曲线下面积为0.977(95%CI 0.958~0.997),敏感性为92.2%,特异性为96.7%。结论DKK-1、RDW可能为发生冠心病的独立危险因素,Wnt-1可能为发生冠心病的独立保护因素。Wnt-1、DKK-1、RDW三者联合高于上述任一指标单独对冠心病的诊断价值。展开更多
目的通过观察香连片对DKK-1 m RNA、β-catenin和PCNA蛋白表达的影响,初步明确香连片预防小鼠溃疡性结肠炎癌变的作用机制。方法 48只BALB/C小鼠随机分为空白组、模型组、香连片用药组和柳氮磺胺嘧啶组,采用DMH/DSS复合法诱导小鼠溃疡...目的通过观察香连片对DKK-1 m RNA、β-catenin和PCNA蛋白表达的影响,初步明确香连片预防小鼠溃疡性结肠炎癌变的作用机制。方法 48只BALB/C小鼠随机分为空白组、模型组、香连片用药组和柳氮磺胺嘧啶组,采用DMH/DSS复合法诱导小鼠溃疡性结肠炎癌变模型,造模时间为18周,用药时间为11周。Real-time PCR检测DKK-1基因的表达,SABC免疫组织化学法检测β-catenin和PCNA蛋白的表达。结果 Real-time PCR检测结果表明香连片可以上调DKK-1 m RNA的表达,与模型组比较,差异有统计学意义(P<0.05);免疫组织化学结果表明香连片可以下调β-catenin和PCNA蛋白的表达,与模型组比较,差异具有统计学意义(P<0.05)。结论香连片预防小鼠溃疡性结肠炎癌变的作用机制与上调DKK-1的表达,阻抑Wnt/β-catenin信号通路有一定的关系。展开更多
文摘Background: Renal osteodystrophy (ROD) is a bone disorder resulting from chronic kidney disease (CKD) and related metabolic diseases. Dickkopf-related protein-1 (DKK-1) is critical in regulating bone biology. This study aimed to evaluate the serum DKK-1 level as a bone marker in children with CKD who undergo regular hemodialysis (HD). Subjects and Methods: This case-control study involved 40 children with CKD on HD and 40 healthy children as controls. The study measured serum DKK-1 levels and performed a dual-energy X-ray absorptiometry scan (DEXA) in line with routine laboratory investigations. Results: There was a significant increase in the serum level of DKK-1 in the patient group compared to the control group. The DKK-1 levels were 2540.65 (2215.4 - 2909.2) pg/ml and 1110.45 (885.45 - 1527.65) pg/ml, respectively, with a p-value of less than 0.001. In the hemodialysis group, 25 patients (62.5%) had low bone mineral density (BMD) with a Z-score of under -2.0. Eighteen of these patients had low BMD in both the neck of the femur and lumbar spines. Additionally, there was a significant increase in serum DKK-1 level in patients with low BMD (2567.35 (2303.8 - 3108.1) pg/ml) compared to patients with normal BMD (2454 (1859 - 2820) pg/ml) (p = 0.041). There was also a significant positive correlation between DKK1 level and phosphorus, alkaline phosphatase, and Parathormone serum levels. In conclusion, the study indicates a clear correlation between DKK-1 and BMD in children undergoing maintenance hemodialysis. DKK1 is a promising biomarker for CKD-MBD.
文摘目的探讨Wnt-1、Dickkopf相关蛋白1(DKK-1)、红细胞分布宽度(red cell distribution width,RDW)与冠心病的相关性,并分析Wnt-1、DKK-1和RDW之间的内在联系及其对冠心病的联合诊断价值。方法选取行冠状动脉(简称冠脉)造影的住院患者188例,根据冠脉造影结果将其分为冠心病组(n=128)和非冠心病组(n=60)并收集临床资料,比较两组间的Wnt-1、DKK-1、RDW差异。结果冠心病组患者血清Wnt-1水平降低,而DKK-1、RDW升高,与非冠心病组相比差异均有统计学意义(P<0.05);冠心病组中的急性心肌梗死组(30例)、不稳定型心绞痛组(54例)和稳定型心绞痛组(44例)三个亚组间Wnt-1、DKK-1及RDW差异均无统计学意义(P>0.05)。经Logistic回归分析,Wnt-1、DKK-1、RDW的OR值分别为0.947、1.023、64.995。三者联合诊断冠心病的受试者工作特征曲线下面积为0.977(95%CI 0.958~0.997),敏感性为92.2%,特异性为96.7%。结论DKK-1、RDW可能为发生冠心病的独立危险因素,Wnt-1可能为发生冠心病的独立保护因素。Wnt-1、DKK-1、RDW三者联合高于上述任一指标单独对冠心病的诊断价值。
文摘目的通过观察香连片对DKK-1 m RNA、β-catenin和PCNA蛋白表达的影响,初步明确香连片预防小鼠溃疡性结肠炎癌变的作用机制。方法 48只BALB/C小鼠随机分为空白组、模型组、香连片用药组和柳氮磺胺嘧啶组,采用DMH/DSS复合法诱导小鼠溃疡性结肠炎癌变模型,造模时间为18周,用药时间为11周。Real-time PCR检测DKK-1基因的表达,SABC免疫组织化学法检测β-catenin和PCNA蛋白的表达。结果 Real-time PCR检测结果表明香连片可以上调DKK-1 m RNA的表达,与模型组比较,差异有统计学意义(P<0.05);免疫组织化学结果表明香连片可以下调β-catenin和PCNA蛋白的表达,与模型组比较,差异具有统计学意义(P<0.05)。结论香连片预防小鼠溃疡性结肠炎癌变的作用机制与上调DKK-1的表达,阻抑Wnt/β-catenin信号通路有一定的关系。
