Contractions of the Lie algebras d = u(2), f = u(1 ,1) to the oscillator Lie algebra l are realized via the adjoint action of SU(2,2) when d, l, f are viewed as subalgebras of su(2,2). Here D, L, F are the correspondi...Contractions of the Lie algebras d = u(2), f = u(1 ,1) to the oscillator Lie algebra l are realized via the adjoint action of SU(2,2) when d, l, f are viewed as subalgebras of su(2,2). Here D, L, F are the corresponding (four-dimensional) real Lie groups endowed with bi-invariant metrics of Lorentzian signature. Similar contractions of (seven-dimensional) isometry Lie algebras iso(D), iso(F) to iso(L) are determined. The group SU(2,2) acts on each of the D, L, F by conformal transformation which is a core feature of the DLF-theory. Also, d and f are contracted to T, S-abelian subalgebras, generating parallel translations, T, and proper conformal transformations, S (from the decomposition of su(2,2) as a graded algebra T + Ω + S, where Ω is the extended Lorentz Lie algebra of dimension 7).展开更多
本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信...本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信号肽、亚细胞定位、亲疏水性、二级结构、三级结构等进行预测。结果表明:成功克隆获得一条长度为1 098 bp的Dlf3h基因,由366个氨基酸组成;该基因在根中表达量最高,其次为叶,在茎中表达量最低;生物信息学分析表明Dlf3h没有信号肽和跨膜结构,是一种可溶性亲水蛋白,定位于细胞核内;二级结构元件主要由α-螺旋和无规则卷曲组成,具有卷曲螺旋;Ramachandran评估表明应用SWISS-MODEL构建的三级结构模型可靠,其配体结合位点为217 His和275 His。本研究结果为进一步研究龙眼类黄酮合成的分子调控机制提供依据。展开更多
文摘Contractions of the Lie algebras d = u(2), f = u(1 ,1) to the oscillator Lie algebra l are realized via the adjoint action of SU(2,2) when d, l, f are viewed as subalgebras of su(2,2). Here D, L, F are the corresponding (four-dimensional) real Lie groups endowed with bi-invariant metrics of Lorentzian signature. Similar contractions of (seven-dimensional) isometry Lie algebras iso(D), iso(F) to iso(L) are determined. The group SU(2,2) acts on each of the D, L, F by conformal transformation which is a core feature of the DLF-theory. Also, d and f are contracted to T, S-abelian subalgebras, generating parallel translations, T, and proper conformal transformations, S (from the decomposition of su(2,2) as a graded algebra T + Ω + S, where Ω is the extended Lorentz Lie algebra of dimension 7).
文摘本研究应用聚合酶链式反应(PCR)技术对龙眼黄烷酮-3-羟化酶基因(Dlf3h)进行克隆;运用实时荧光定量PCR (Quantitative real time PCR)技术对Dlf3h基因在龙眼根、茎、叶组织中的表达进行分析;采用生物信息学方法对Dlf3h蛋白理化性质、信号肽、亚细胞定位、亲疏水性、二级结构、三级结构等进行预测。结果表明:成功克隆获得一条长度为1 098 bp的Dlf3h基因,由366个氨基酸组成;该基因在根中表达量最高,其次为叶,在茎中表达量最低;生物信息学分析表明Dlf3h没有信号肽和跨膜结构,是一种可溶性亲水蛋白,定位于细胞核内;二级结构元件主要由α-螺旋和无规则卷曲组成,具有卷曲螺旋;Ramachandran评估表明应用SWISS-MODEL构建的三级结构模型可靠,其配体结合位点为217 His和275 His。本研究结果为进一步研究龙眼类黄酮合成的分子调控机制提供依据。
