Objective To compare the quality and quantity of total RNA from different source-original neurons applied in LMPC technique. Methods （ 1 ） Aglient 2100 bioanalyzer and RT-PCR were used to check the concentration and...Objective To compare the quality and quantity of total RNA from different source-original neurons applied in LMPC technique. Methods （ 1 ） Aglient 2100 bioanalyzer and RT-PCR were used to check the concentration and fragmentation of total RNA from unfixed, temporal fixed and fixed 12 h hypothalamus sections; （2）Different neurons of PVN and SON were collected by LMPC, CRH, TRH, AVP, OT mRNA level were measured by RT-PCR; （3）Labeled neurons by injecting CTB into stomach and non-labeled neurons in DMV collected by LMPC were checked for house keeping genes by RT-PCR. Results （ 1 ） Unfixed section had higher concentration and better quality of total RNA compared with fixed sections applied in LMPC ; relative short amplicons such as GAPDH, NSE, MCH and MCAR were successfully obtained from fixed and unfixed and long amplicon of GR can only be obtained from unfixed material; （2） In magnocellular PVN and SON the expressions of AVP and OT were more special than those in the parvocellular PVN. Oppositely, the expressions of CRH, TRH in the parvocellular were more special than the other two ; （3） The expressions of house keeping genes had no significant difference between labeled and non-labeled DMV neurons. Conclusion The quality and quantity of total RNA from unfixed brain tissues were better than fixed tissues applied in LMPC and the CTB tracer which may differentiate neurons had no significant effect on physiology of the neurons applied in LMPC. The results showed that the LMPC technique is suitable for the qualitative and quantitative study on individual neurons at mRNA level.展开更多
该文以单电感双输出(SIDO)Boost变换器为研究对象,详细分析电感电流工作于连续导电模式(CCM)的共模-差模电压型(CMV-DMV)控制SIDO Boost变换器的工作原理。采用时间平均等效电路建模方法,推导主电路的控制-输出、输出阻抗、交叉影响阻...该文以单电感双输出(SIDO)Boost变换器为研究对象,详细分析电感电流工作于连续导电模式(CCM)的共模-差模电压型(CMV-DMV)控制SIDO Boost变换器的工作原理。采用时间平均等效电路建模方法,推导主电路的控制-输出、输出阻抗、交叉影响阻抗等传递函数。在此基础上,建立CMV-DMV控制CCM SIDO Boost变换器的闭环小信号模型,并利用Bode图从频域的角度分析变换器两条输出支路在不同输出电压等级下的交叉影响特性。研究结果表明,在两路输出电压不等时,CMV-DMV控制CCM SIDO Boost变换器的高压输出支路对低压输出支路的交叉影响较小;在两路输出电压相等时,先导通输出支路对后导通输出支路的交叉影响较大。实验结果验证了理论分析的正确性。展开更多
文摘Objective To compare the quality and quantity of total RNA from different source-original neurons applied in LMPC technique. Methods （ 1 ） Aglient 2100 bioanalyzer and RT-PCR were used to check the concentration and fragmentation of total RNA from unfixed, temporal fixed and fixed 12 h hypothalamus sections; （2）Different neurons of PVN and SON were collected by LMPC, CRH, TRH, AVP, OT mRNA level were measured by RT-PCR; （3）Labeled neurons by injecting CTB into stomach and non-labeled neurons in DMV collected by LMPC were checked for house keeping genes by RT-PCR. Results （ 1 ） Unfixed section had higher concentration and better quality of total RNA compared with fixed sections applied in LMPC ; relative short amplicons such as GAPDH, NSE, MCH and MCAR were successfully obtained from fixed and unfixed and long amplicon of GR can only be obtained from unfixed material; （2） In magnocellular PVN and SON the expressions of AVP and OT were more special than those in the parvocellular PVN. Oppositely, the expressions of CRH, TRH in the parvocellular were more special than the other two ; （3） The expressions of house keeping genes had no significant difference between labeled and non-labeled DMV neurons. Conclusion The quality and quantity of total RNA from unfixed brain tissues were better than fixed tissues applied in LMPC and the CTB tracer which may differentiate neurons had no significant effect on physiology of the neurons applied in LMPC. The results showed that the LMPC technique is suitable for the qualitative and quantitative study on individual neurons at mRNA level.
文摘该文以单电感双输出(SIDO)Boost变换器为研究对象,详细分析电感电流工作于连续导电模式(CCM)的共模-差模电压型(CMV-DMV)控制SIDO Boost变换器的工作原理。采用时间平均等效电路建模方法,推导主电路的控制-输出、输出阻抗、交叉影响阻抗等传递函数。在此基础上,建立CMV-DMV控制CCM SIDO Boost变换器的闭环小信号模型,并利用Bode图从频域的角度分析变换器两条输出支路在不同输出电压等级下的交叉影响特性。研究结果表明,在两路输出电压不等时,CMV-DMV控制CCM SIDO Boost变换器的高压输出支路对低压输出支路的交叉影响较小;在两路输出电压相等时,先导通输出支路对后导通输出支路的交叉影响较大。实验结果验证了理论分析的正确性。
基金Project supported by the National Natural Science Foundation of China （Nos. 51209052, 51279038, and 51479041）, the Natural Sci- ence Foundation of Heilongjiang Province （No. QC2011C013）, and the Opening Funds of State Key Laboratory of Ocean Engineering of Shanghai Jiao Tong University （No. 1307）, China
