Tumor necrosis factor-alpha(TNF-α) is a type of critical pro-inflammatory cytokines,which participates in numerous cellular signal pathways and is regarded as a critical protein biomarker for inflammatory based disea...Tumor necrosis factor-alpha(TNF-α) is a type of critical pro-inflammatory cytokines,which participates in numerous cellular signal pathways and is regarded as a critical protein biomarker for inflammatory based diseases.In this contribution,we have developed a strategy to fabricate multiple DNA star trigon structures with fluorescence signals from four hairpin probes which are detonated by a single molecule of TNF-α.This process causes significant enhancement of fluorescence and a sensitive and selective biosensor for TNF-α assay is constructed.This method is able to achieve the limit of detection(LOD) at 5 pg/mL(0.285 pM).Moreover,some other advantages such as fast response,high selectivity and convenient operation promise the potential use of this method for TNF-α measurement in point of care testing application.Upon further development,this strategy can also be converted to detect other analytes such as small molecules,nucleic acids and other proteins.展开更多
The possibility of the life origin in the stellar systems, located at a distance of ~200 pc from the solar system, was investigated. The stars, in the spectrums of which C (carbon), O (oxygen), N (nitrogen), and P (ph...The possibility of the life origin in the stellar systems, located at a distance of ~200 pc from the solar system, was investigated. The stars, in the spectrums of which C (carbon), O (oxygen), N (nitrogen), and P (phosphorus) are found, are called DNA-stars. Based on stellar abundances a new method for searching for habitable exoplanets has been developed and a list of 48 DNA-stars in the solar neighborhood, on which life is possible, has been defined. The quota of DNA-stars is equal 1.3% of the total amount of Hypatia Stellar Catalog. Only three DNA-stars out of selected 48 stars belong to the spectral class as our Sun (G2V). The closest to the solar system is the DNA-star with the number HIP 15510, which belongs to the G8V class and is 6 pc away from the solar system. Nine DNA-stars, which have the highest chemical similarity with solar spectrum, were identified. It is identified that one of these nine stars, HIP 24681, has six planets.展开更多
Chemical elements in space can be synthesized by stellar nuclear reactors. Studying the dynamics of processes occurring in the stars introduces a concept of the ensemble-averaged stellar reactor. For future interstell...Chemical elements in space can be synthesized by stellar nuclear reactors. Studying the dynamics of processes occurring in the stars introduces a concept of the ensemble-averaged stellar reactor. For future interstellar missions, the terrestrial and solar abundances were compared with considerable number of stars allocated in the ~200 pc solar neighborhood. According to the value of the effective temperature, four stellar classes are distinguished, for which the correlation coefficients and standard deviation are calculated. The statement about the possibility of transferring heavy elements synthesized by stars over long distances in space has been completely refuted. There is no immutability of the distribution of elements on neighboring stars and in the Solar System. It is shown that chemical elements are mainly synthesized inside each stellar reactor. The theory of the buoyancy of elements is generalized to stars. It has been suggested that stars overheat due to a shift in the parameters of nuclear processes occurring inside stars, which leads to the synthesis of heavy elements.展开更多
对阳江豆豉分离菌株进行多项分类鉴定,并对该菌中性蛋白酶基因克隆和序列分析,预测菌株中性蛋白酶二级结构。采用18SrRNA基因序列鉴定菌株,并以菌株中性蛋白酶的氨基酸序列为基础,应用DNA Star Protean软件对蛋白酶的二级结构进行预测...对阳江豆豉分离菌株进行多项分类鉴定,并对该菌中性蛋白酶基因克隆和序列分析,预测菌株中性蛋白酶二级结构。采用18SrRNA基因序列鉴定菌株,并以菌株中性蛋白酶的氨基酸序列为基础,应用DNA Star Protean软件对蛋白酶的二级结构进行预测。通过形态和18SrRNA基因序列分析,鉴定该菌株为米曲霉Aspergillus oryzae。通过RT-PCR和PCR从菌丝体中克隆了基因序列,与产中性蛋白酶有关的核苷酸序列有2 113bp,推测出634个氨基酸序列。展开更多
Discrete and symmetric three-dimensional（3D） DNA nanocages have been revoked as excellent candidates for various applications,such as guest component encapsulation and organization（e.g.dye molecules,proteins,inorga...Discrete and symmetric three-dimensional（3D） DNA nanocages have been revoked as excellent candidates for various applications,such as guest component encapsulation and organization（e.g.dye molecules,proteins,inorganic nanoparticles,etc.） to construct new materials and devices.To date,a large variety of DNA nanocages has been synthesized through assembling small individual DNA motifs into predesigned structures in a bottom-up fashion.Most of them rely on the assembly using multiple copies of single type of motifs and a few sophisticated nanostructures have been engineered by co-assembling multi-types of DNA tiles simultaneously.However,the availability of complex DNA nanocages is still limited.Herein,we demonstrate that highly symmetric DNA nanocages consisted of binary DNA pointstar motifs can be easily assembled by deliberately engineering the sticky-end interaction between the component building blocks.As such,DNA nanocages with new geometries,including elongated tetrahedron（E-TET）,rhombic dodecahedron（R-DOD）,and rhombic triacontahedron（R-TRI） are successfully synthesized.Moreover,their design principle,assembly process,and structural features are revealed by polyacryalmide gel electrophoresis（PAGE）,atomic force microscope（AFM） imaging,and cryogenic transmission electron microscope imaging（cryo-TEM） associated with single particle reconstruction.展开更多
基金supported by the National Key Instrument Developing Project of China(ZDYZ2013-1)the National Natural Science Foundation of China(31400847)the Natural Science Foundation of Jiangsu Province of China(BK20141204)
文摘Tumor necrosis factor-alpha(TNF-α) is a type of critical pro-inflammatory cytokines,which participates in numerous cellular signal pathways and is regarded as a critical protein biomarker for inflammatory based diseases.In this contribution,we have developed a strategy to fabricate multiple DNA star trigon structures with fluorescence signals from four hairpin probes which are detonated by a single molecule of TNF-α.This process causes significant enhancement of fluorescence and a sensitive and selective biosensor for TNF-α assay is constructed.This method is able to achieve the limit of detection(LOD) at 5 pg/mL(0.285 pM).Moreover,some other advantages such as fast response,high selectivity and convenient operation promise the potential use of this method for TNF-α measurement in point of care testing application.Upon further development,this strategy can also be converted to detect other analytes such as small molecules,nucleic acids and other proteins.
文摘The possibility of the life origin in the stellar systems, located at a distance of ~200 pc from the solar system, was investigated. The stars, in the spectrums of which C (carbon), O (oxygen), N (nitrogen), and P (phosphorus) are found, are called DNA-stars. Based on stellar abundances a new method for searching for habitable exoplanets has been developed and a list of 48 DNA-stars in the solar neighborhood, on which life is possible, has been defined. The quota of DNA-stars is equal 1.3% of the total amount of Hypatia Stellar Catalog. Only three DNA-stars out of selected 48 stars belong to the spectral class as our Sun (G2V). The closest to the solar system is the DNA-star with the number HIP 15510, which belongs to the G8V class and is 6 pc away from the solar system. Nine DNA-stars, which have the highest chemical similarity with solar spectrum, were identified. It is identified that one of these nine stars, HIP 24681, has six planets.
文摘Chemical elements in space can be synthesized by stellar nuclear reactors. Studying the dynamics of processes occurring in the stars introduces a concept of the ensemble-averaged stellar reactor. For future interstellar missions, the terrestrial and solar abundances were compared with considerable number of stars allocated in the ~200 pc solar neighborhood. According to the value of the effective temperature, four stellar classes are distinguished, for which the correlation coefficients and standard deviation are calculated. The statement about the possibility of transferring heavy elements synthesized by stars over long distances in space has been completely refuted. There is no immutability of the distribution of elements on neighboring stars and in the Solar System. It is shown that chemical elements are mainly synthesized inside each stellar reactor. The theory of the buoyancy of elements is generalized to stars. It has been suggested that stars overheat due to a shift in the parameters of nuclear processes occurring inside stars, which leads to the synthesis of heavy elements.
文摘对阳江豆豉分离菌株进行多项分类鉴定,并对该菌中性蛋白酶基因克隆和序列分析,预测菌株中性蛋白酶二级结构。采用18SrRNA基因序列鉴定菌株,并以菌株中性蛋白酶的氨基酸序列为基础,应用DNA Star Protean软件对蛋白酶的二级结构进行预测。通过形态和18SrRNA基因序列分析,鉴定该菌株为米曲霉Aspergillus oryzae。通过RT-PCR和PCR从菌丝体中克隆了基因序列,与产中性蛋白酶有关的核苷酸序列有2 113bp,推测出634个氨基酸序列。
基金financially supported by the National Natural Science Foundation of China(Nos.21504053,21673139,and91527304)the Program of Shanghai Medical Professionals Across Subject Funds(No.YG2016MS74)the Recruitment Program of Global Experts(No.15Z127060012)
文摘Discrete and symmetric three-dimensional（3D） DNA nanocages have been revoked as excellent candidates for various applications,such as guest component encapsulation and organization（e.g.dye molecules,proteins,inorganic nanoparticles,etc.） to construct new materials and devices.To date,a large variety of DNA nanocages has been synthesized through assembling small individual DNA motifs into predesigned structures in a bottom-up fashion.Most of them rely on the assembly using multiple copies of single type of motifs and a few sophisticated nanostructures have been engineered by co-assembling multi-types of DNA tiles simultaneously.However,the availability of complex DNA nanocages is still limited.Herein,we demonstrate that highly symmetric DNA nanocages consisted of binary DNA pointstar motifs can be easily assembled by deliberately engineering the sticky-end interaction between the component building blocks.As such,DNA nanocages with new geometries,including elongated tetrahedron（E-TET）,rhombic dodecahedron（R-DOD）,and rhombic triacontahedron（R-TRI） are successfully synthesized.Moreover,their design principle,assembly process,and structural features are revealed by polyacryalmide gel electrophoresis（PAGE）,atomic force microscope（AFM） imaging,and cryogenic transmission electron microscope imaging（cryo-TEM） associated with single particle reconstruction.
