Cytogenetic characterization of olive flounder Paralichthys olivaceus: DNA content, karyotype, AgNORs and location of major ribosomal genes

A cytogenetic analysis of Paralichthys olivaceus was carried out using the flow cytometry method for DNA content, silver staining for the nucleolus organizer region （AgNORs） identification and one-color fluorescence in situ hybridization （FISH） for chromosomal mapping of major ribosomal genes. Nuclear DNA content was estimated by flow cytometry method using Gallus domesticus erythrocytes as the internal reference standard. The C-value of this species was （0.737±0.024） pg, and the DNA contents of each chromosome were estimated to be 16.51 Mb to 39.50 Mb after paired according to the average relative length. The FISH probe was made by PCR amplification of a DNA fragment containing internal transcribed spacers ITS1 between 18S and 5.8S ribosomal RNA gene, and labeled by PCR incorporation of bio-16-dUTP. FISH signals and AgNORs were both located on the secondary constrictions of chromosome 1. These results will provide a better understanding of the cytogenetic information of this species and would help for further research of the karyotype evolution in the order Pleuronectiformes.

Analysis of DNA Content of Various Types of Spermatogenic Cells in Rat after Testicular Heating with Flow Cytometry

To measure DNA contents of spermatogenic cells and analyze the efficiency of spermatogenesis after testicular heating in rat Methods Eighty adult male Sprague-Dawley rats were randomly divided into experimental group （43 ℃, 30 min） and control group （22 ℃, 30 min）. According to day 0.5, 1, 3, 6, 10, 25, 35 and 50 after local testicular heating, every group was divided into 8 subgroups： experimental subgroups （n=6） and control subgroups （n=4）. DNA contents of various types of germ cells were observed with flow cytometry （FCM） in all groups. Results Compared with control groups, percentages of 4C cell （primary spermatocyte） in 0.5-35 d groups and percentages of 1C cell （spermatid and sperm） in 6-50 d groups significantly decreased in experimental groups （P〈0.05）, and percentages of 2C cell （spermatogonium and second spermatocyte） in 3 -35 d experimental groups increased significantly after heating （P〈0.05）. 4C：2C in all of 8 experimental groups and 1C：2C in 3-35 d experimental groups were down （P〈0. 05）, and in 1 d experimental group 1C：4C was up after heating （P〈0.05）. Conclusions After being heated, the number of spermatocyte firstly decreased, and then that of spermatid and sperm decreased too. Heat influences several stages in spermatogenesis and results in suppression of spermatogenesis. Flow cytometry is an effective method for researching on the change of spermatogenesis and has significance on mechanism about changing of spermatogenic cells induced by heat.

DNA Content in Pancreatic ExocrinalCells after Vagotom yand Electron Microscopy in Rats

Fifty six SD rats were randomly divided into the normal control group and the operation group. The operating group was subdivided into six groups on the basis of killing time (the 12th h, the first day, 3rd day, first week, 2nd week and 4th week) after vagotomy (VG). The pancreatic tissues were taken for HE and Feulgen staining. The DNA content of pancreatic exocrine cells was determined by a domestically fabricated computer image analyzing system. In the control group, on the first day or in the first week after VG, the pancreatic samples were taken for transmission electron microscopic examination. The DNA content of pancreatic exocrinal cells was decreased from 1 to 3 days after VG. The secretion was found to be in inhibitory state and One week later, it gradually restored. The results indicated that the proliferation and the function of the SD rat's pancreatic exocrinal cells were prohibited at initial stage after VG, which might be concerned, at least in part, with dominance and nutrition of vagus.

EFFECTS OF PERIOPERATIVE CIMETIDINE ADMINISTRATION ON TUMOR CELL NUCLEAR MORPHOMETRY AND DNA CONTENT IN PATIENTS WITH GASTROINTESTINAL CANCER

Objective: To explore the effects of perioperative cimetidine administration on tumor cell nuclear morphometric parameters and DNA content in patients with gastrointestinal adenocarcinoma. Methods: 49 patients with pathologically confirmed gastrointestinal adenocarcinoma were randomized into test group (n=25) and control group (n=24). The test group started oral cimetidine intake 400 mg, tid, 7–10d before operation, followed by standard curative operation. The control group did not receive cimetidine. Tumor specimens were paraffin embedded for microsection and stained with hematoxylin and eosin (HE) and Feulgen stain. Morphometric studies and DNA content of tumor nuclei were performed on IBAS Image Analyzer. Results: The tumor cell nuclear area (μm2), nuclear perimeter (μm), maximal nuclear diameter (μ) for test group/control group were 23.54 5.08/34.69110.08 (P<0.001), 22.064.43/24.884.05 (P<0.05), 7.8411.64/ 8.6211.24 (P<0.05), 4.4210.61/5.4110.89 (P<0.001), Respectively. The percentages (%) of diploidy, triple-tetraploidy, quintuple ploidy, and >quintuple ploidy tumor cells for test group/control group were 16.6412.58/5.3312.14 (P<0.002), 39.8412.28/35.7013.58 (P>0.50), 12.4215.00/14.4810.74 (P>0.20), 31.1116.86/ 45.9713.82 (P<0.005), respectively. Conclusion: Perioperative administration of cimetidine in gasgtrointestinal cancer patients could decrease the nuclear size and raise the percentage of diploid tumor cells, and convert high aneuploid tumor cells into low-aneuploid tumor cells, which might help reduce the invasiveness of tumor cells.

Flow Cytometry Analysis of Cellular DNA Content of the Parotid Pleomorphic Adenoma

The nuclear DNA content in 42 cases of primary parotid pleomorphic adenoma (PPA) and 15 cases of recurrent PPA were analysed with flowcytometer (FCM) to study the correlation of the DNA index (DI), S% and cellular proliferous index (PI) with the biologic behaivor of the tumor. The results indicated that in a portion of pleomorphic adenoma, the nuclear DNA content had altered and possessed malignant potential before any evidence of malignancy could be found under lightmicroscope. The increase of S% and PI, which followed the course of tumor development, has a close relation with the capsular invasion and the recurrence. There was a significant difference on the DI, S%, PI as well as the incidence of heteroploid between the recurrent and the incipient tumor. A great portion of recurrent tumors, which still diagnosed as benign pathologically, was acually heteroploid.

Conserved balance of hepatocyte nuclear DNA content in mononuclear and binuclear hepatocyte populations during the course of chronic viral hepatitis

AIM： TO analyze the percentages of hepatocytes with increased nuclear DNA content, i.e., tetraploid （4n） and octoploid （Sn） nuclei, and then compared mononuclear and binuclear hepatocyte populations： METHODS： The percentages of mononuclear diploid （2n）, 4n, and 8n hepatocytes and those of binuclear 2 × 2n, 2 × 4n, and 2 × 8n hepatocytes were determined with a method that can simultaneously measure hepatocyte nuclear DNA content and binuclearity in 62 patients with chronic hepatitis B or C. The percentage of 4n and 8n hepatocytes in the mononuclear hepatocyte population was compared with the percentage of 2 × 4n and 2 × 8n hepatocytes in the binuclear hepatocyte population. RESULTS： The percentages of 4n and 8n hepatocytes in mononuclear hepatocytes and 2 ×4n and 2 × 8n hepatocytes in binuclear hepatocytes were similar, regardless of the activity or fibrosis grade of chronic hepatitis and regardless of the infecting virus. CONCLUSION： The distribution of nuclear DNA content within mononuclear and binuclear hepatocyte populations was conserved during the course of chronic viral hepatitis.

Effect of A-L Tonic Capsule on DNA Content in Rat Experimental Hepatocarcinogenesis

The effects of A L tonic capsule on DNA content in rat experimental hepatocarcinogenesis induced by diethylnitrosamine (DENA) were observed. The experimental rats were divided into 4 groups. With exception of group D in which the rats were only administered with DENA, the rats in the groups A, B, C were previously, simultaneously and subsequently fed with A L tonic capsule respectively while they were administered with DENA. The DNA content of all rat livers was measured using automatic image analysis technique 20 weeks after administration of DENA. The results showed that the highest and lowest DNA contents were respectively seen in the groups D and A. There was significant difference in DNA contents between the groups A or B or C and D, and also between the groups A and B or C (both P <0.01). 4 components (4C) and ≥5C cells were predominant in the group D, while 2C cells were the minority. The number of 2C cells in the groups A, B, C was significantly higher than that in the group D, and the number of ≥5C cells in the groups A, B, C was markedly lower than that in the group D ( P <0.01). Also, there was very significant difference in the number of 2C and ≥5C cells between group A and B or C ( P <0.01). It was concluded that A L tonic capsule could effectively inhibit the increase of DNA content of hepatocytes and improve the distribution of DNA content in rat hepatocarcinogenesis, especially in group A.

QUANTITATIVE STUDIES ON THE DNA CONTENT AND MORPHOMETRIC FEATURES OF LIVER CELL DYSPLASIA

The DNA content and morphometric features of hepatocellular carcinoma (HCC) and liver cell dysplasia (LCD), including nuclear area, nuclear perimeter, nuclear maximum diameter and nuclear circle diameter, were quantitatively determined by means of image analysis technology. The results showed that in comparison with normal hepatocytes, LCD had a markedly increased DNA content and nuclear morphometric parameters, but the values were lower than those for HCC. LCD showed a slight increase in nuclear atypia represented by the nuclear irregular index, which was also less than HCC. The findings indicate that LCD may be a precaneerous lesion of HCC, to the cells in an abnormal proliferative state.

DETERMINATION OF NUCLEAR DNA CONTENT IN LEIOMYOMA AND LEIOMYOSARCOMA OF GASTROINTESTINAL TRACT

Nuclear DNA content was measured by micro-spec trophotometry in 15 biopsy specimens from patients with gastrointestinal (GI) tract smooth muscle tumors (3 leiomyomas and 12 leiomyosarco-mas subdivided into 3 groups with 4 cases to each). The mean DNA value increased steadily as follows: leiomyoma (14.39±0.62 Au); leiomyosarcoma Grade Ⅰ (19.78±2.39 Au); leiomyosarcoma Grade Ⅱ (26.39± 1.60 Au); leiomyosarcoma Grade Ⅲ (30.66±2.39 Au). The difference of DNA value in the 4 groups had statistical significance (p<0.05-0.01). These results suggest that microspectrophotometric measurement of nuclear DNA content may serve as an objective quantitative method for the diagnosis of GI tract smooth muscle tumors and classification of leiomyosarcoma.

FLOW CYTOM ETRIC ANALYSIS OF CELLULAR DNA CONTENT IN EPITHELIAL OVARIAN TUMOR

The DNA content of tumor all was analyzed by flow cytometry on parafflnembedded specimens in 73 patients with epithelial ovarian tumor, and its clinical significance was evaluated. One of the 5 benign (20%), 2 of the 11 borderline (18.18%), and 30 of the 57 malignant (52. 63%) tumors were aneuplold. The occurrence rate of aneuploidy In malignant tumors was higher than In benign and borderline tumors ( P < 0. 05 ). Furthermore, aneuploidy was more frequently In the advanced stages (Ⅲ -Ⅳ ) (77. 7%) than in the early stages (Ⅰ - Ⅱ ) (9. 5%) (P<0. 005). The occurrence rate of DNA aneuploidy was higher in patients associated with ascites and the residual tumor≥.2 cm. Patients with aneuploid tumors had more of ten ascites (P<0. 005) and residual tumor size≥2cm (P< 0.005). There was no apparent correlation between the DNA ptoidy and the histologic grade, histologic type of the tumors. G0/G1 cell proportion of DNA diplold tumors in advanced carcinoma (64. 6%) was less than those of early stage carcinoma (75. 9% ) (P<0. 05). The survival rate of diplold tumor patients was higher than that of aneuploid tumor patients in the different time after operation, and the median survival time was 30. 2 months and 10. 3 months, respectively. Multivariate analysis revealed that cellular DNA ploidy was the most Important predictive factor (P = 0. 007) of prognosis, followed by residual tumor size (P= 0. 05). Different tumor specimen of the same patient can exhibit variation sometime (38. 9%).The results revealed that the DNA ploidy may reflect tumor biological characteristics, I. e. , Its proliferative ability. Analysis of cellular DNA content of epithelial ovarian tumors would help us to predict the prognosis of the patients better.

Magnetic fields-induced modification of DNA content in date palm （Phoenix dactylifera L.）

Plant stress caused by exposure to magnetic fields （MF） induces modifications at molecular level, particularly in DNA synthesis, structure, and function. The objective of this study was to determine the effect of various doses of non-ionizing radiation of magnetic field on date palm （Phoenix dactylifera L.） based on DNA content. Date palm seedlings （cv. Khalas） established for 2 weeks on filter paper were subjected to static magnetic field and alternating magnetic field. Static magnetic fields （SMF） were applied at 10, 50 and 100 mT for 30, 60, 120, 180, 240 and 360 min; while alternating magnetic field applied by magnetic resonance imaging （MRI） at 1500 mT for 1, 5, 10 and 15 min. The seedlings were grown in potting soil following exposure for 4 weeks after which DNA was extracted from leaves and its content was determined. Generally, the exposure to magnetic field caused reduction in the content of DNA. The lowest exposure time tested, 30 rain, was sufficient to induce reduction in DNA content. This was true even at the lowest intensity, 10 mT. This dosage caused the DNA content to decrease from 49 ~tg/g to 45 pg/g. Further, increase of the exposure duration to 60 min caused significant reduction in the DNA content, 36 μg/g. At intensities higher than 10 mT, DNA content decreased significantly even at the shortest exposure of 30 min. At 50 and 100 mT, significant decrease in DNA content was also noticed in response to 30 min exposure; whereas the level of DNA increased after 1 min of MRI exposure to 52 ~g/g, then decreased after 5 min to 46 ~g/g. However, longer durations caused no further decrease in the DNA content. These observations indicate that magnetic fields interact with DNA processes, probably by inhibiting synthesis or stimulating degradation of DNA. This response merits further exploration as a mutational agent for date palm genetic manipulation.

The Value of p21^(WAF1), p53 Protein and DNA Content in Large Intestine Cancer

目的 探讨 p2 1WAF1 蛋白和 p5 3蛋白在大肠癌发生发展中的作用及其临床意义。方法 对 4 1例大肠腺癌标本及 10例正常大肠粘膜标本 ,应用免疫组织化学S -P法检测细胞周期调控因子 p2 1WAF1 蛋白和突变型 p5 3蛋白 ,应用流式细胞术检测新鲜标本DNA含量。结果 p2 1WAF1 与 p5 3表达呈负相关 ;在正常大肠粘膜、高分化腺癌、中分化腺癌、低分化腺癌中 p5 3表达率逐渐增高 ,p2 1WAF1 表达率逐渐降低 ,SPF值逐渐升高 ;Dukes分期越晚 ,p2 1WAF1 表达率越低 ,p5 3表达率越高 ,SPF越高。结论 本实验证实了 p2 1WAF1 和 p5 3表达在肿瘤发生发展中起着调控点的作用 ;p2 1WAF1 、p5

硒对云南白灵芝生长、品质及DNA甲基化特征的影响

采用田间覆土栽培试验,设置6个Se浓度(0、50、100、200、300、400μg/g)处理,分别记为CK、Se50、Se100、Se200、Se300、Se400,研究外源硒对云南白灵芝生长发育、重金属积累、生物活性成分含量及其DNA甲基化特征的影响。结果表明,与CK相比,外源Se对子实体柄长和菌盖大小(横径、纵径)无显著影响,但能显著降低菌盖厚度;随着Se施用水平提高,菌丝生长速率和子实体硒含量均呈先增后降的趋势,生物量呈先降后增再降的趋势。子实体重金属含量(As、Cd、Pb、Hg)随Se浓度增加呈持续降低趋势,线性分析也表明,重金属含量与Se施用浓度均呈显著负相关。施Se增加了子实体多糖、粗蛋白、黄酮及总氨基酸含量,其中,多糖、黄酮含量在Se400处理下最高,总氨基酸、粗蛋白含量分别在Se200、Se300处理下最高。HPLC测定分析表明,与CK相比,Se300提高了灵芝酸A、灵芝酸DM、灵芝酸F及灵芝酮三醇为主的三萜酸组分,其总三萜酸含量较其他处理提高2.93%~25.54%。敏感扩增多态性分析(MSAP)结果显示,Se300较CK、Se400相比具有较高的未甲基化位点比例和较低的甲基化位点比例。综上,施用外源硒可促进早期菌丝生长,提高子实体生物量累积,改善子实体重金属安全性能和生物活性成分品质,改变DNA甲基化比例,且以300μg/g施用浓度的整体效果最佳,其子实体产量较对照显著提高11.36%。

脱落细胞DNA定量分析技术动态监测口腔扁平苔藓伴上皮异常增生病情及光动力治疗1例

口腔扁平苔藓是一种临床上常见的慢性炎性口腔黏膜病,具有癌变风险,临床上应积极干预并随访监测。光动力治疗具有微创、有效、选择性好以及可重复性高等优势,在治疗口腔扁平苔藓中已显现出良好疗效,但其疗效评估方法仍然存在局限性。脱落细胞DNA定量分析技术是一种无创辅助检查手段,可用于口腔潜在恶性疾患癌变及口腔癌的早筛早诊。本文报道1例采用脱落细胞DNA定量分析技术动态监测口腔扁平苔藓癌变风险及光动力治疗疗效的诊疗过程,以期为建立口腔扁平苔藓伴上皮异常增生的精准微创诊疗体系提供参考。

基于水体及胃含物DNA宏条形码技术的斑鳠幼鱼食性分析

为摸清斑鳠(Mystus guttatus)幼鱼的食性和生物学特征,为其资源保护和种群恢复提供科学依据,采用DNA宏条形码技术分析了贵州省罗甸县斑鳠保种场河道中的斑鳠幼鱼胃含物及水体的物种特征。结果显示:1)在斑鳠幼鱼胃含物中共鉴定出109种生物,分属于15个门,12大类。从门水平看,节肢动物门相对丰度最高,其次是轮虫动物门、绿藻门、脊索动物门。从属水平看,中镖水蚤属(Sinodiaptomus)相对丰度最高,其次是臂尾轮虫属(Brachionus)、鲃属(Barbus)和Paralamyctes。2)在斑鳠幼鱼生存的河道中,共获得193个物种,分属于18个门。从门水平来看,节肢动物门相对丰度最高,其次是绿藻门。从属水平来看,中镖水蚤属的相对丰度最高,其次是衣藻属(Chlamydomonas),占比较高的还有臂尾轮虫属、筒壳虫属(Tintinnidium)和单壳缝藻属(Monoraphidium)。节肢动物门、轮虫动物门和绿藻门在斑鳠幼鱼胃含物中和水体中都是相对丰度较高的类群。在水体中,脊索动物门相对丰度较低(第15位),而在胃含物中列第4位。研究揭示了斑鳠幼鱼食性选择受水域环境食物的易得性及其喜好性两方面的影响。从能量收支效益的角度来看,斑鳠幼鱼在摄食过程中遵循以最小的能量投入获取最大收益的原则,利于幼鱼存活与生长。

基于流式细胞术的蜘蛛抱蛋属植物倍性检测和核DNA含量测定体系的建立

为建立适用于蜘蛛抱蛋属植物的倍性检测及其核DNA含量的流式细胞术方法,以蜘蛛抱蛋属植物成熟叶片为材料,比较了WPB解离液、LB01解离液、Galbraith’s解离液以及改良Galbraith’s解离液制备的细胞核悬液的效果。结果表明:改良Galbraith’s解离液能够更广泛地适用于蜘蛛抱蛋属植物成熟叶片的细胞核悬液的制备且效果较好。利用改良Galbraith’s解离液对12种13个居群的蜘蛛抱蛋属植物进行倍性检测,并通过染色体制片计数法进行验证。结果表明:12种蜘蛛抱蛋属植物的DNA含量的荧光强度峰值范围为1753078.81~5817826.99。其中,11种二倍体植物的峰值范围为1753078.81~2937690.80,四倍体辐花蜘蛛抱蛋的峰值为3892503.69,六倍体泰国蜘蛛抱蛋的峰值为5817826.99。此外,以葱为内标,对以上蜘蛛抱蛋属植物的核DNA含量进行测定和估算,首次估测了11种蜘蛛抱蛋植物的核DNA含量,蜘蛛抱蛋的DNA含量与已有报道的结果相近。其中,二倍体植物的核DNA含量为14.16~18.73 Gb;四倍体辐花蜘蛛抱蛋的核DNA含量为28.33 Gb;六倍体泰国蜘蛛抱蛋的核DNA含量为43.03 Gb。结果可为蜘蛛抱蛋属植物的遗传育种研究、全基因组研究以及喀斯特植物的起源与演化研究提供重要科学依据。

利用基于Python/RGB模块的DNA电泳图像分析方法检测绵羊血浆中羊源性成分

该文探究了运用Python处理食品中DNA分子量与含量测定的新方法,建立了DNA凝胶图像分析方法。将不同分子量的DNA Marker与DNA标准样品进行琼脂糖凝胶电泳并拍照,利用自行开发Python程序分析凝胶图,对图像进行灰度图转换、高斯模糊、图像阈值化、轮廓检测的图像优化步骤,探究了轮廓平均值法、轮廓中线法、全局数据平均法、全局数据积分法反映出DNA浓度与RGB数值间的线性关系,选取最优处理方法,通过读取像素迁移距离、RGB-灰度、RGB-向量、RGB-亮度进行DNA分子量与含量的测定实验,建立一种基于Python/RGB色彩体系的DNA凝胶电泳中分子量与含量的分析方法。检测结果误差较小,证明了Python/RGB的DNA分子量与含量分析方法的可行性,同时将该文所构建的凝胶图像分析方法应用于绵羊血浆中羊源性成分检测,结果显示所得目的蛋白为296 bp,而用DNA检测法得出样品中片段大小为294 bp,误差为0.99%,有望构建一种肉类源性成分检测的新方法。

基于DNA宏条形码技术的杭州萧山国际机场家燕春夏食性分析及防治策略

2022—2023年期间,杭州萧山国际机场累计鉴定鸟击残留物样本154例,涉及54种鸟类,其中家燕(Hirundo rustica)为出现频率最高的鸟种,共检测到9次,鸟击风险较高。为降低家燕对飞行安全的威胁,利用DNA宏条形码技术(DNA metabarcoding technology)检测肠道内容物,对机场春夏两季的家燕进行食性分析。同时结合机场调查数据,有效地掌握了家燕的食源分布。结合机场实地调研数据,研究结果发现麻蝇科、果蝇科、蚊科、丽蝇科、蝇科和灰蝶科都属于动物性食物,其中麻蝇科为主要食物,而滋养食源的相关植物有蔷薇科、禾本科、伞形科、千屈菜科、桑科、榆科、百合科和柏科,蔷薇科则是主要滋养物。通过α多样性指数比较发现,动物性食物多样性在夏季显著高于春季(Shannon指数,P<0.05),群落物种分布更均匀,而与家燕食源相关的植物丰富度在春季则显著高于夏季(Chao1和Observed_species指数,P<0.05),造成这种差异动态的因素可能是机场区域温度变化。通过β多样性指数比较发现,动物性食物和食物链相关植物组成差异在春、夏季不明显。针对家燕的食性组成以及食物资源的分布模式,我们建议机场应提高收割植物籽实频率,扩大收割面积,排查家燕营巢区域,规范水稻、大豆等作物种植,妥善处理机场垃圾,定期喷洒杀虫剂,设立昆虫诱捕装置,清理植草区虫卵虫蛹,以降低家燕鸟击事件的发生概率。与传统食性分析相比,通过DNA宏条形码技术比较了不同季节食性,补充了目前发现的家燕食物组成,并且对机场的鸟击防范政策实施具有指导意义。

高效液相色谱-示差折光法测定NMM抗肿瘤DNA疫苗中甘露醇含量

目的 建立测定NMM抗肿瘤DNA疫苗中甘露醇含量的高效液相色谱-示差折光法。方法 检测器为示差折光检测器,色谱柱为SUGAR SC1011阳离子钙型交换柱(300 mm×8 mm,6μm),流动相为纯化水,流速为1.0 mL/min,柱温为80℃,检测池温度为37℃,进样量为10μL。结果 甘露醇的质量浓度在0.02~5.00 mg/mL(R2=1.000 0,n=6)范围内与峰面积线性关系良好;定量限为13.37μg/mL;精密度、重复性、稳定性试验结果的RSD均小于2.0%;加样回收率为97.76%,RSD为0.97%(n=9)。3批小试样品和中试样品中甘露醇的含量分别为20.02,19.95 mg/mL。结论 该方法操作简便、重复性好、结果准确,可用于NMM抗肿瘤DNA疫苗中甘露醇的含量测定。

乙肝大小三阳状态、不同HBVDNA载量孕妇肝功能指标水平分析

目的研究探讨乙肝孕妇大三阳和小三阳状态中乙型肝炎病毒(Hepatitis B virus,HBV)DNA载量、肝功能指标、肝纤维化标志物含量及不同HBVDNA载量孕妇的肝功能水平。方法选取临清市人民医院于2020年1月—2023年1月收治的120例乙肝孕妇作为研究对象,根据乙肝两对半检测结果将其分为两组,即大三阳组(n=60)和小三阳组(n=60)。比较两组HBVDNA载量、肝功能指标、肝纤维化标志物含量及不同HBVDNA载量孕妇的肝功能指标、肝纤维化标志物含量。结果大三阳组丙氨酸转移酶水平(29.00±7.62)U/L、HBVDNA水平(5.18±1.16)×10^(4)U/mL均高于小三阳组的(26.00±2.85)U/L、(2.08±1.11)×10^(4)U/mL,差异有统计学意义(t=2.856、14.956,P均<0.05)。大三阳组的重组人几丁质酶3样蛋白1水平低于小三阳组,差异有统计学意义(P<0.05)。伴随着HBVDNA载量上升,两组患者甲胎蛋白水平随之增加,但是在同等HBADNA载量下,两组甲胎蛋白水平比较,差异无统计学意义(P>0.05)。结论开展孕前抗乙肝病毒诊疗、孕期风险系数评估,采取科学有效的干预措施,均能够有效抑制乙肝在母婴中的传播。