In order to study the molecular recognition ability of DNA and different behavior of dyes incorporated into the base pairs, DNA molecule was assembled layer by layer via a Zr(Ⅳ) ion. The UV absorption spectra showed ...In order to study the molecular recognition ability of DNA and different behavior of dyes incorporated into the base pairs, DNA molecule was assembled layer by layer via a Zr(Ⅳ) ion. The UV absorption spectra showed the uniform layer assembly of the DNA film. The fabricated DNA film was water-insoluble and maintained the native B-form structure. UV and CD measurements showed that the DNA film could intercalate ethidium bromide (EtBr) .展开更多
We present our results from the measurements of third-order optical nonlinearity in DNA doped Rhodamine 6G/PVA films achieved through Z-scan measurements using ~2 picosecond (ps) pulses at a wavelength of 800 nm. The ...We present our results from the measurements of third-order optical nonlinearity in DNA doped Rhodamine 6G/PVA films achieved through Z-scan measurements using ~2 picosecond (ps) pulses at a wavelength of 800 nm. The films demonstrated negative nonlinear refractive index (n2) with magnitudes of (0.065 - 2.89) × 10–14 cm2/W with varying concentration of DNA. Open aperture data demonstrated strong two-photon absorption with a magnitude of ~1.6 cm/GW for films doped with 2 wt% of DNA. The recovery time of excited state population, retrieved from the degenerate pump-probe experimental data, was <4 ps. These data suggests that DNA is promising material for applications such as optical switching.展开更多
Titania thin films with hierarchically macro-mesoporous were prepared by the addition of bulky organic molecule(1,3,5-trimethylbezenze) in aqueous solution of F127(EO106PO70EO106). After calcined at 673 K,ordered thin...Titania thin films with hierarchically macro-mesoporous were prepared by the addition of bulky organic molecule(1,3,5-trimethylbezenze) in aqueous solution of F127(EO106PO70EO106). After calcined at 673 K,ordered thin films with cubic meso-structure and macropores were formed. DNA(Cytochrome c) was used as probe to study the adsorption ability of macromolecules on this film. The hierarchically macro-mesoporous films show high adsorption ability of DNA molecules compared with conventional titania films and mesoporous titania films,which is attributed to the presence of macropores.展开更多
A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethylaminopropyl）-N’-et...A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethylaminopropyl）-N’-ethylcarbodiimide hydrochloride（EDC）and N-hydroxysuccinimide（NHS）.By using methylene blue as the electrochemical indicator,the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8 1.0×10-4 mol/L with the linear regression equation as ΔI pc=1.10lgc+8.25（n=8,γ=0.998 5）and the detection limit as 3.60×10-8 mol/L（3σ）.展开更多
文摘In order to study the molecular recognition ability of DNA and different behavior of dyes incorporated into the base pairs, DNA molecule was assembled layer by layer via a Zr(Ⅳ) ion. The UV absorption spectra showed the uniform layer assembly of the DNA film. The fabricated DNA film was water-insoluble and maintained the native B-form structure. UV and CD measurements showed that the DNA film could intercalate ethidium bromide (EtBr) .
文摘We present our results from the measurements of third-order optical nonlinearity in DNA doped Rhodamine 6G/PVA films achieved through Z-scan measurements using ~2 picosecond (ps) pulses at a wavelength of 800 nm. The films demonstrated negative nonlinear refractive index (n2) with magnitudes of (0.065 - 2.89) × 10–14 cm2/W with varying concentration of DNA. Open aperture data demonstrated strong two-photon absorption with a magnitude of ~1.6 cm/GW for films doped with 2 wt% of DNA. The recovery time of excited state population, retrieved from the degenerate pump-probe experimental data, was <4 ps. These data suggests that DNA is promising material for applications such as optical switching.
文摘Titania thin films with hierarchically macro-mesoporous were prepared by the addition of bulky organic molecule(1,3,5-trimethylbezenze) in aqueous solution of F127(EO106PO70EO106). After calcined at 673 K,ordered thin films with cubic meso-structure and macropores were formed. DNA(Cytochrome c) was used as probe to study the adsorption ability of macromolecules on this film. The hierarchically macro-mesoporous films show high adsorption ability of DNA molecules compared with conventional titania films and mesoporous titania films,which is attributed to the presence of macropores.
文摘A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethylaminopropyl）-N’-ethylcarbodiimide hydrochloride（EDC）and N-hydroxysuccinimide（NHS）.By using methylene blue as the electrochemical indicator,the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8 1.0×10-4 mol/L with the linear regression equation as ΔI pc=1.10lgc+8.25（n=8,γ=0.998 5）and the detection limit as 3.60×10-8 mol/L（3σ）.
