由致病疫霉Phytophthora infestans （Mont） de Bary引起的晚疫病是马铃薯生产上可导致绝收的世界性病害,在我国各地均有发生和流行[1]。传统的致病型鉴别只局限于表型上的分析,无法对复杂多变的晚疫病菌致病型变化进行快速准确的预测...由致病疫霉Phytophthora infestans （Mont） de Bary引起的晚疫病是马铃薯生产上可导致绝收的世界性病害,在我国各地均有发生和流行[1]。传统的致病型鉴别只局限于表型上的分析,无法对复杂多变的晚疫病菌致病型变化进行快速准确的预测,因此有必要探索新的方法来弥补常规手段的不足,以便更加准确有效地监测其变化趋势。晚疫病菌与寄主马铃薯这一病理体系中的单一抗性基因和相应的无毒基因符合典型＂基因对基因＂学说,展开更多
In the present study, formalin-fixed feces, oli-gonucleotide fingerprinting and SRY-gene based sexing were used to construct a family net for giant pandas in the Tang-jiahe Natural Reserve and to assess contemporary g...In the present study, formalin-fixed feces, oli-gonucleotide fingerprinting and SRY-gene based sexing were used to construct a family net for giant pandas in the Tang-jiahe Natural Reserve and to assess contemporary gene flow (migration) in this population. A total of 124 fecal samples were attributed to 37 individuals (22 females and 15 males) that were then analyzed for family relationships. Based on DNA fingerprints, the deduced family net revealed the fol-lowing facts: (i) First-order relatives possessed similarities from 50% to 90%, and similarities between unrelated indi-viduals or distant relatives were as high as 77%, indicating that the Tangjiahe pandas are characterized by high genetic similarity; (ii) 15 matings were identified and 5 ones oc-curred between close relatives, implying that there is poten-tial for inbreeding to impact the pandas; (iii) four mating pairs and 5 offspring presented long distance migrations, demonstrating the intra-reserve habitat is continuous; (iv) four pairs of full sibs (also female-male dyads) dispersed short distance and all of them gave birth to highly inbred offspring, reflecting long distance migration is vital for in-breeding avoidance; (v) 17 adult individuals dispersed short or moderate distance and formed three clusters on the land-scape, indicating that it is necessary to find out whether there is a negative factor impacting the pandas.展开更多
文摘由致病疫霉Phytophthora infestans （Mont） de Bary引起的晚疫病是马铃薯生产上可导致绝收的世界性病害,在我国各地均有发生和流行[1]。传统的致病型鉴别只局限于表型上的分析,无法对复杂多变的晚疫病菌致病型变化进行快速准确的预测,因此有必要探索新的方法来弥补常规手段的不足,以便更加准确有效地监测其变化趋势。晚疫病菌与寄主马铃薯这一病理体系中的单一抗性基因和相应的无毒基因符合典型＂基因对基因＂学说,
基金This work was supported by the National Science Fund for Distinguished Young Scholars(Grant No.30325009)the State Key Basic Research and Development Plan of the P.R.China(Grant No.G2000046906)+1 种基金a special grant for the giant panda from the Sichuan Forestry DepartmentState Forestry Administration of China.
文摘In the present study, formalin-fixed feces, oli-gonucleotide fingerprinting and SRY-gene based sexing were used to construct a family net for giant pandas in the Tang-jiahe Natural Reserve and to assess contemporary gene flow (migration) in this population. A total of 124 fecal samples were attributed to 37 individuals (22 females and 15 males) that were then analyzed for family relationships. Based on DNA fingerprints, the deduced family net revealed the fol-lowing facts: (i) First-order relatives possessed similarities from 50% to 90%, and similarities between unrelated indi-viduals or distant relatives were as high as 77%, indicating that the Tangjiahe pandas are characterized by high genetic similarity; (ii) 15 matings were identified and 5 ones oc-curred between close relatives, implying that there is poten-tial for inbreeding to impact the pandas; (iii) four mating pairs and 5 offspring presented long distance migrations, demonstrating the intra-reserve habitat is continuous; (iv) four pairs of full sibs (also female-male dyads) dispersed short distance and all of them gave birth to highly inbred offspring, reflecting long distance migration is vital for in-breeding avoidance; (v) 17 adult individuals dispersed short or moderate distance and formed three clusters on the land-scape, indicating that it is necessary to find out whether there is a negative factor impacting the pandas.
