To investigate a contribution of O-methylguanineto mutagenesis in mouse cells,we constructed ashuttle vector plasmid,pYZ289,from a part ofpZ189 plasmid and polyoma virus DNA.The plasmidcontains a supF gene as a marker...To investigate a contribution of O-methylguanineto mutagenesis in mouse cells,we constructed ashuttle vector plasmid,pYZ289,from a part ofpZ189 plasmid and polyoma virus DNA.The plasmidcontains a supF gene as a marker of mutation andcan replicate in both E.coli and mouse cells.ThepYZ289 treated with N-methyl-N-nitrosourea (MNU)展开更多
The molecular mechanism of DNA damage induced by hydroquinone （HQ） remains unclear. Poly（ADP-ribose） polymerase-1 （PARP-1） usually works as a DNA damage sensor, and hence, it is possible that PARP-1 is involved ...The molecular mechanism of DNA damage induced by hydroquinone （HQ） remains unclear. Poly（ADP-ribose） polymerase-1 （PARP-1） usually works as a DNA damage sensor, and hence, it is possible that PARP-1 is involved in the DNA damage response induced by HQ. In TK6 cells treated with HQ, PARP activity as well as the expression of apoptosis antagonizing transcription factor （AATF）, PARP-1, and phosphorylated H2AX （v-H2AX） were maximum at 0.5 h, 6 h, 3 h, and 3 h, respectively. To explore the detailed mechanisms underlying the prompt DNA repair reaction, the above indicators were investigated in PARP-l-silenced cells. PARP activity and expression of AATF and PARP-1 decreased to 36%, 32%, and 33%, respectively, in the cells; however, y-H2AX expression increased to 265%. Co-immunoprecipitation （co-IP） assays were employed to determine whether PARP-1 and AATF formed protein complexes. The interaction between these proteins together with the results from IP assays and confocal microscopy indicated that poly（ADP-ribosyl）ation {PARylation） regulated AATF expression, in conclusion, PARP-1 was involved in the DNA damage repair induced by HQ via increasing the accumulation of AATF through PARylation.展开更多
With the Unscheduied DNA Synthesls (UDS),We studiedorganotropism of DNA damage in different organ cells ofrat induced by Alternariol (AOH) and Alternariol mono-methyl ether (AME) produced by Alternaria alternata thatw...With the Unscheduied DNA Synthesls (UDS),We studiedorganotropism of DNA damage in different organ cells ofrat induced by Alternariol (AOH) and Alternariol mono-methyl ether (AME) produced by Alternaria alternata thatwas preponderate strain isolated from the grain in thehigh incidence area of esophageal cancer.The展开更多
24 0rganophosphates(0PPs) were tested by Salmonella test(SAL),B.subtilis DNA repair test(SUB),mouse micronucleustest(MNT-M),CHL cell micronucleus test(MNT-C) and SOSinduction test(S0S).The results of 33 0PPs in SCE te...24 0rganophosphates(0PPs) were tested by Salmonella test(SAL),B.subtilis DNA repair test(SUB),mouse micronucleustest(MNT-M),CHL cell micronucleus test(MNT-C) and SOSinduction test(S0S).The results of 33 0PPs in SCE test invitro from literatures were also analysed.The results werecontradicted to those reported by Fahrig and Klopman,thatis not only the methyl-0PPs shown genetic activity or highprobability of positive reaction,but the ethyl-0PPs in pre-sent studies,also demostrated high positive reaction rate.展开更多
The methods of in vitro and in vivo-in vitro combinationassay for autoradiographic detection of unscheduled DNAsynthesis(UDS)induced by chemical carcinogens wasestablished.To estimate the reliability of these assays,U...The methods of in vitro and in vivo-in vitro combinationassay for autoradiographic detection of unscheduled DNAsynthesis(UDS)induced by chemical carcinogens wasestablished.To estimate the reliability of these assays,UDS in tissues slices was measured with the establishedmethods after exposure to MNNG and dimethylnitrosamine(DMN)in vitro or in vivo respectively.To study thesusceptibility of various tissues to chemicals,UDS展开更多
文摘To investigate a contribution of O-methylguanineto mutagenesis in mouse cells,we constructed ashuttle vector plasmid,pYZ289,from a part ofpZ189 plasmid and polyoma virus DNA.The plasmidcontains a supF gene as a marker of mutation andcan replicate in both E.coli and mouse cells.ThepYZ289 treated with N-methyl-N-nitrosourea (MNU)
基金supported by grants from the National Natural Science Foundation of China(8120223181273116+2 种基金81430079)the Science and Technology Program of Guangdong Bureau of Science and TechnologyChina(2013B021800069)
文摘The molecular mechanism of DNA damage induced by hydroquinone （HQ） remains unclear. Poly（ADP-ribose） polymerase-1 （PARP-1） usually works as a DNA damage sensor, and hence, it is possible that PARP-1 is involved in the DNA damage response induced by HQ. In TK6 cells treated with HQ, PARP activity as well as the expression of apoptosis antagonizing transcription factor （AATF）, PARP-1, and phosphorylated H2AX （v-H2AX） were maximum at 0.5 h, 6 h, 3 h, and 3 h, respectively. To explore the detailed mechanisms underlying the prompt DNA repair reaction, the above indicators were investigated in PARP-l-silenced cells. PARP activity and expression of AATF and PARP-1 decreased to 36%, 32%, and 33%, respectively, in the cells; however, y-H2AX expression increased to 265%. Co-immunoprecipitation （co-IP） assays were employed to determine whether PARP-1 and AATF formed protein complexes. The interaction between these proteins together with the results from IP assays and confocal microscopy indicated that poly（ADP-ribosyl）ation {PARylation） regulated AATF expression, in conclusion, PARP-1 was involved in the DNA damage repair induced by HQ via increasing the accumulation of AATF through PARylation.
文摘With the Unscheduied DNA Synthesls (UDS),We studiedorganotropism of DNA damage in different organ cells ofrat induced by Alternariol (AOH) and Alternariol mono-methyl ether (AME) produced by Alternaria alternata thatwas preponderate strain isolated from the grain in thehigh incidence area of esophageal cancer.The
文摘24 0rganophosphates(0PPs) were tested by Salmonella test(SAL),B.subtilis DNA repair test(SUB),mouse micronucleustest(MNT-M),CHL cell micronucleus test(MNT-C) and SOSinduction test(S0S).The results of 33 0PPs in SCE test invitro from literatures were also analysed.The results werecontradicted to those reported by Fahrig and Klopman,thatis not only the methyl-0PPs shown genetic activity or highprobability of positive reaction,but the ethyl-0PPs in pre-sent studies,also demostrated high positive reaction rate.
文摘The methods of in vitro and in vivo-in vitro combinationassay for autoradiographic detection of unscheduled DNAsynthesis(UDS)induced by chemical carcinogens wasestablished.To estimate the reliability of these assays,UDS in tissues slices was measured with the establishedmethods after exposure to MNNG and dimethylnitrosamine(DMN)in vitro or in vivo respectively.To study thesusceptibility of various tissues to chemicals,UDS
