[Objective]The aim was to introduce T-DNA into watermelon for its molecular marker research.[Method]Based on the method of foreign DNA introduced to Arabidopsis thaliana via dipping flowers,the stigma smear was used t...[Objective]The aim was to introduce T-DNA into watermelon for its molecular marker research.[Method]Based on the method of foreign DNA introduced to Arabidopsis thaliana via dipping flowers,the stigma smear was used to transfer T-DNA into watermelon and its molecular marker research was carried out.[Result]The ideal transformed species was ZXG01078 for the highest fruit setting rate and the most deviant seedlings.The best concentration of kanamycin for treating watermelon seeds was 500-700 mg/L with differences among the species.The best position was spire leaf or young leaf and the best concentration of kanamycin for treating the watermelon leaf was 4 000-8 000 mg/L with no significant difference among species.The steadily variation appearing of growing pointless and conjoined twin seedlings indicated that the normal growth had been interfered by foreign DNA in the progeny.[Conclusion]This study had provided basis for the further research on watermelon.展开更多
Acacia species are found at various arid and semiarid regions. Among the tree genera of family, Fabaceae, Acacia contains the highest number of species. We have collected different species of Acacia from various place...Acacia species are found at various arid and semiarid regions. Among the tree genera of family, Fabaceae, Acacia contains the highest number of species. We have collected different species of Acacia from various places of Saudi Arabia and reconstructed phylogeny for evaluation of genetic relationship among them. Internal transcribed spacer sequence of nrDNA (nrDNA-ITS) locus was used for the reconstruction of phylogeny among these species. Based on phylogenetic tree, Acacia etbaica and A. johnwoodii were close to each other. Similarly, A. ehrenbergiana and A. tortilis were close to each other. Thus, this gene locus was helpful in evaluating the genetic relationship among these species.展开更多
基金Supported by Crop Germplasm Resources Protection Project of Ministry of Agriculture(NB08-2130135-44)National Science and Technology Infrastructure Project(2005DKA21001-23)
文摘[Objective]The aim was to introduce T-DNA into watermelon for its molecular marker research.[Method]Based on the method of foreign DNA introduced to Arabidopsis thaliana via dipping flowers,the stigma smear was used to transfer T-DNA into watermelon and its molecular marker research was carried out.[Result]The ideal transformed species was ZXG01078 for the highest fruit setting rate and the most deviant seedlings.The best concentration of kanamycin for treating watermelon seeds was 500-700 mg/L with differences among the species.The best position was spire leaf or young leaf and the best concentration of kanamycin for treating the watermelon leaf was 4 000-8 000 mg/L with no significant difference among species.The steadily variation appearing of growing pointless and conjoined twin seedlings indicated that the normal growth had been interfered by foreign DNA in the progeny.[Conclusion]This study had provided basis for the further research on watermelon.
文摘Acacia species are found at various arid and semiarid regions. Among the tree genera of family, Fabaceae, Acacia contains the highest number of species. We have collected different species of Acacia from various places of Saudi Arabia and reconstructed phylogeny for evaluation of genetic relationship among them. Internal transcribed spacer sequence of nrDNA (nrDNA-ITS) locus was used for the reconstruction of phylogeny among these species. Based on phylogenetic tree, Acacia etbaica and A. johnwoodii were close to each other. Similarly, A. ehrenbergiana and A. tortilis were close to each other. Thus, this gene locus was helpful in evaluating the genetic relationship among these species.
